Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_K12
(723 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P34455|ACON_CAEEL Probable aconitate hydratase, mitochon... 354 1e-97
sp|O13966|ACON_SCHPO Aconitate hydratase, mitochondrial pre... 340 2e-93
sp|P16276|ACON_PIG Aconitate hydratase, mitochondrial precu... 337 1e-92
sp|Q9ER34|ACON_RAT Aconitate hydratase, mitochondrial precu... 336 4e-92
sp|Q99798|ACON_HUMAN Aconitate hydratase, mitochondrial pre... 335 1e-91
sp|Q99KI0|ACON_MOUSE Aconitate hydratase, mitochondrial pre... 334 1e-91
sp|P20004|ACON_BOVIN Aconitate hydratase, mitochondrial pre... 334 2e-91
sp|P49609|ACON_GRAVE Aconitate hydratase, mitochondrial pre... 325 1e-88
sp|P19414|ACON_YEAST Aconitate hydratase, mitochondrial pre... 318 1e-86
sp|P39533|ACON2_YEAST Putative aconitase in PRP21-UBP12 int... 290 4e-78
>sp|P34455|ACON_CAEEL Probable aconitate hydratase, mitochondrial precursor (Citrate
hydro-lyase) (Aconitase)
Length = 777
Score = 354 bits (909), Expect = 1e-97
Identities = 174/238 (73%), Positives = 198/238 (83%)
Frame = +2
Query: 2 FPGVLLIGTDSHTPNXXXXXXXXXXXXXADAVDVMADIPWELKCPRVIGVRLTGRLSGWT 181
FPG+LLIGTDSHTPN ADAVDVMADIPWELKCP+VIG++LTG+L+GWT
Sbjct: 180 FPGLLLIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGIKLTGKLNGWT 239
Query: 182 SSKDVILKVADILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPFNER 361
S+KDVILKVADILTVKGGTGAIVEYFG GVDSIS TGMGTICNMGAEIGATTSVFP+NE
Sbjct: 240 SAKDVILKVADILTVKGGTGAIVEYFGPGVDSISATGMGTICNMGAEIGATTSVFPYNES 299
Query: 362 MMEYLVATNRADIGAEAARYKEALLTADRNCKYDQVIEIDLSTLQPHINGPFTPDLAHPI 541
M +YL AT R +I EA +YK+ LLTAD YDQ+IEI+L TL PH+NGPFTPDLA I
Sbjct: 300 MYKYLEATGRKEIAEEARKYKD-LLTADDGANYDQIIEINLDTLTPHVNGPFTPDLASSI 358
Query: 542 AEMGKRAKQNGWPERISVGLIGSCTNSSYEDMTRAASIAKQAVDRGLRSKCAFTITPG 715
++G+ AK+NGWP + V LIGSCTNSSYEDMTRAASIAKQA+D+GL++K FTITPG
Sbjct: 359 DKLGENAKKNGWPLDVKVSLIGSCTNSSYEDMTRAASIAKQALDKGLKAKTIFTITPG 416
>sp|O13966|ACON_SCHPO Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 778
Score = 340 bits (872), Expect = 2e-93
Identities = 165/238 (69%), Positives = 194/238 (81%)
Frame = +2
Query: 2 FPGVLLIGTDSHTPNXXXXXXXXXXXXXADAVDVMADIPWELKCPRVIGVRLTGRLSGWT 181
FPG LLIGTDSHTPN ADAVDVMA++PWELKCP+VIGV+LTG+L GWT
Sbjct: 179 FPGGLLIGTDSHTPNAGGLGMVAIGVGGADAVDVMANLPWELKCPKVIGVKLTGQLKGWT 238
Query: 182 SSKDVILKVADILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPFNER 361
S KDVILKVA ILTVKGGTGAIVEYFG GV+S+SCTGMGTICNMGAEIGATTS+FPFN R
Sbjct: 239 SPKDVILKVAGILTVKGGTGAIVEYFGPGVESLSCTGMGTICNMGAEIGATTSIFPFNPR 298
Query: 362 MMEYLVATNRADIGAEAARYKEALLTADRNCKYDQVIEIDLSTLQPHINGPFTPDLAHPI 541
M EYL ATNR+ I A+ A ++ AD N YDQ+IEIDL+TL+PH+NGPFTPDLA PI
Sbjct: 299 MSEYLRATNRSAI-ADYAEEFAPIIAADENAHYDQIIEIDLNTLEPHLNGPFTPDLATPI 357
Query: 542 AEMGKRAKQNGWPERISVGLIGSCTNSSYEDMTRAASIAKQAVDRGLRSKCAFTITPG 715
++ + K+N WP+ + VGLIGSCTNSSYEDM+RAASI +QA+D+G+++K FTITPG
Sbjct: 358 SKFKEAVKKNDWPQELKVGLIGSCTNSSYEDMSRAASICQQAIDKGIKTKSLFTITPG 415
>sp|P16276|ACON_PIG Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 781
Score = 337 bits (865), Expect = 1e-92
Identities = 170/238 (71%), Positives = 189/238 (79%)
Frame = +2
Query: 2 FPGVLLIGTDSHTPNXXXXXXXXXXXXXADAVDVMADIPWELKCPRVIGVRLTGRLSGWT 181
+PGVLLIGTDSHTPN ADAVDVMA IPWELKCP+VIGV+LTG LSGWT
Sbjct: 183 YPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWT 242
Query: 182 SSKDVILKVADILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPFNER 361
S KDVILKVA ILTVKGGTGAIVEY G GVDSISCTGM TICNMGAEIGATTSVFP+N R
Sbjct: 243 SPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHR 302
Query: 362 MMEYLVATNRADIGAEAARYKEALLTADRNCKYDQVIEIDLSTLQPHINGPFTPDLAHPI 541
M +YL T RADI A +K+ L+ D C YDQVIEI+LS L+PHINGPFTPDLAHP+
Sbjct: 303 MKKYLSKTGRADIANLADEFKDHLV-PDPGCHYDQVIEINLSELKPHINGPFTPDLAHPV 361
Query: 542 AEMGKRAKQNGWPERISVGLIGSCTNSSYEDMTRAASIAKQAVDRGLRSKCAFTITPG 715
AE+G A++ GWP I VGLIGSCTNSSYEDM R+A++AKQA+ GL+ K FTITPG
Sbjct: 362 AEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPG 419
>sp|Q9ER34|ACON_RAT Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 336 bits (861), Expect = 4e-92
Identities = 168/238 (70%), Positives = 190/238 (79%)
Frame = +2
Query: 2 FPGVLLIGTDSHTPNXXXXXXXXXXXXXADAVDVMADIPWELKCPRVIGVRLTGRLSGWT 181
+PGVLLIGTDSHTPN ADAVDVMA IPWELKCP+VIGV+LTG LSGWT
Sbjct: 183 YPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGTLSGWT 242
Query: 182 SSKDVILKVADILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPFNER 361
S KDVILKVA ILTVKGGTGAIVEY G GVDSISCTGM TICNMGAEIGATTSVFP+N R
Sbjct: 243 SPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHR 302
Query: 362 MMEYLVATNRADIGAEAARYKEALLTADRNCKYDQVIEIDLSTLQPHINGPFTPDLAHPI 541
M +YL T RADI A +K+ L+ D C+YDQVIEI+L+ L+PHINGPFTPDLAHP+
Sbjct: 303 MKKYLSKTGRADIANLAEEFKDHLV-PDPGCQYDQVIEINLNELKPHINGPFTPDLAHPV 361
Query: 542 AEMGKRAKQNGWPERISVGLIGSCTNSSYEDMTRAASIAKQAVDRGLRSKCAFTITPG 715
A++G A++ GWP I VGLIGSCTNSSYEDM R+A++AKQA+ GL+ K FTITPG
Sbjct: 362 ADVGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPG 419
>sp|Q99798|ACON_HUMAN Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 335 bits (858), Expect = 1e-91
Identities = 168/238 (70%), Positives = 189/238 (79%)
Frame = +2
Query: 2 FPGVLLIGTDSHTPNXXXXXXXXXXXXXADAVDVMADIPWELKCPRVIGVRLTGRLSGWT 181
+PGVLLIGTDSHTPN ADAVDVMA IPWELKCP+VIGV+LTG LSGW+
Sbjct: 183 YPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWS 242
Query: 182 SSKDVILKVADILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPFNER 361
S KDVILKVA ILTVKGGTGAIVEY G GVDSISCTGM TICNMGAEIGATTSVFP+N R
Sbjct: 243 SPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHR 302
Query: 362 MMEYLVATNRADIGAEAARYKEALLTADRNCKYDQVIEIDLSTLQPHINGPFTPDLAHPI 541
M +YL T R DI A +K+ L+ D C YDQ+IEI+LS L+PHINGPFTPDLAHP+
Sbjct: 303 MKKYLSKTGREDIANLADEFKDHLV-PDPGCHYDQLIEINLSELKPHINGPFTPDLAHPV 361
Query: 542 AEMGKRAKQNGWPERISVGLIGSCTNSSYEDMTRAASIAKQAVDRGLRSKCAFTITPG 715
AE+GK A++ GWP I VGLIGSCTNSSYEDM R+A++AKQA+ GL+ K FTITPG
Sbjct: 362 AEVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPG 419
>sp|Q99KI0|ACON_MOUSE Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 334 bits (857), Expect = 1e-91
Identities = 167/238 (70%), Positives = 189/238 (79%)
Frame = +2
Query: 2 FPGVLLIGTDSHTPNXXXXXXXXXXXXXADAVDVMADIPWELKCPRVIGVRLTGRLSGWT 181
+PGVLLIGTDSHTPN ADAVDVMA IPWELKCP+VIGV+LTG LSGWT
Sbjct: 183 YPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWT 242
Query: 182 SSKDVILKVADILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPFNER 361
S KDVILKVA ILTVKGGTGAIVEY G GVDSISCTGM TICNMGAEIGATTSVFP+N R
Sbjct: 243 SPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHR 302
Query: 362 MMEYLVATNRADIGAEAARYKEALLTADRNCKYDQVIEIDLSTLQPHINGPFTPDLAHPI 541
M +YL T R DI A +K+ L+ D C+YDQVIEI+L+ L+PHINGPFTPDLAHP+
Sbjct: 303 MKKYLSKTGRTDIANLAEEFKDHLV-PDPGCQYDQVIEINLNELKPHINGPFTPDLAHPV 361
Query: 542 AEMGKRAKQNGWPERISVGLIGSCTNSSYEDMTRAASIAKQAVDRGLRSKCAFTITPG 715
A++G A++ GWP I VGLIGSCTNSSYEDM R+A++AKQA+ GL+ K FTITPG
Sbjct: 362 ADVGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPG 419
>sp|P20004|ACON_BOVIN Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 334 bits (856), Expect = 2e-91
Identities = 168/238 (70%), Positives = 188/238 (78%)
Frame = +2
Query: 2 FPGVLLIGTDSHTPNXXXXXXXXXXXXXADAVDVMADIPWELKCPRVIGVRLTGRLSGWT 181
+PGVLLIGTDSHTPN ADAVDV A IPWELKCP+VIGV+LTG LSGWT
Sbjct: 183 YPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVTAGIPWELKCPKVIGVKLTGSLSGWT 242
Query: 182 SSKDVILKVADILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPFNER 361
S KDVILKVA ILTVKGGTGAIVEY G GVDSISCTGM TICNMGAEIGATTSVFP+N R
Sbjct: 243 SPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHR 302
Query: 362 MMEYLVATNRADIGAEAARYKEALLTADRNCKYDQVIEIDLSTLQPHINGPFTPDLAHPI 541
M +YL T RADI A +K+ L+ D C YDQ+IEI+LS L+PHINGPFTPDLAHP+
Sbjct: 303 MKKYLSKTGRADIANLADEFKDHLV-PDSGCHYDQLIEINLSELKPHINGPFTPDLAHPV 361
Query: 542 AEMGKRAKQNGWPERISVGLIGSCTNSSYEDMTRAASIAKQAVDRGLRSKCAFTITPG 715
AE+G A++ GWP I VGLIGSCTNSSYEDM R+A++AKQA+ GL+ K FTITPG
Sbjct: 362 AEVGSVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLQCKSQFTITPG 419
>sp|P49609|ACON_GRAVE Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 779
Score = 325 bits (832), Expect = 1e-88
Identities = 158/238 (66%), Positives = 186/238 (78%)
Frame = +2
Query: 2 FPGVLLIGTDSHTPNXXXXXXXXXXXXXADAVDVMADIPWELKCPRVIGVRLTGRLSGWT 181
FPG+L+IGTDSHTPN ADAVDVM +PWELK P+VIGV+LTG+L W
Sbjct: 181 FPGLLMIGTDSHTPNAGGLGACAVGVGGADAVDVMVGLPWELKAPKVIGVKLTGKLQEWA 240
Query: 182 SSKDVILKVADILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPFNER 361
S KDVILKVA ILTVKGGTGAIVEYFGEGVDS+SCTGMGTICNMGAEIGATTS+FP+N R
Sbjct: 241 SPKDVILKVAGILTVKGGTGAIVEYFGEGVDSLSCTGMGTICNMGAEIGATTSMFPYNSR 300
Query: 362 MMEYLVATNRADIGAEAARYKEALLTADRNCKYDQVIEIDLSTLQPHINGPFTPDLAHPI 541
M +YL AT R I + A + E L AD N YDQ+IEI+LS L+PHINGPFTPDLAHP+
Sbjct: 301 MGDYLKATGRDGIASLADSFSEQ-LRADENAVYDQLIEINLSELEPHINGPFTPDLAHPL 359
Query: 542 AEMGKRAKQNGWPERISVGLIGSCTNSSYEDMTRAASIAKQAVDRGLRSKCAFTITPG 715
++ + ++NGWP ++VGLIGSCTNSSYEDM R+AS+ KQA+ G++SK F ITPG
Sbjct: 360 SKFKEEVEKNGWPAELTVGLIGSCTNSSYEDMARSASVVKQALSHGVKSKSIFNITPG 417
>sp|P19414|ACON_YEAST Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 778
Score = 318 bits (814), Expect = 1e-86
Identities = 153/238 (64%), Positives = 186/238 (78%)
Frame = +2
Query: 2 FPGVLLIGTDSHTPNXXXXXXXXXXXXXADAVDVMADIPWELKCPRVIGVRLTGRLSGWT 181
FPG L+IGTDSHTPN ADAVDVMA PWELK P+++GV+LTG+++GWT
Sbjct: 179 FPGALIIGTDSHTPNAGGLGQLAIGVGGADAVDVMAGRPWELKAPKILGVKLTGKMNGWT 238
Query: 182 SSKDVILKVADILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPFNER 361
S KD+ILK+A I TVKGGTG IVEYFG+GVD+ S TGMGTICNMGAEIGATTSVFPFN+
Sbjct: 239 SPKDIILKLAGITTVKGGTGKIVEYFGDGVDTFSATGMGTICNMGAEIGATTSVFPFNKS 298
Query: 362 MMEYLVATNRADIGAEAARYKEALLTADRNCKYDQVIEIDLSTLQPHINGPFTPDLAHPI 541
M+EYL AT R I A Y + LL+AD++ +YD+V+EIDL+TL+P+INGPFTPDLA P+
Sbjct: 299 MIEYLEATGRGKIADFAKLYHKDLLSADKDAEYDEVVEIDLNTLEPYINGPFTPDLATPV 358
Query: 542 AEMGKRAKQNGWPERISVGLIGSCTNSSYEDMTRAASIAKQAVDRGLRSKCAFTITPG 715
++M + A N WP + VGLIGSCTNSSYEDM+R+ASI K A GL+SK FT+TPG
Sbjct: 359 SKMKEVAVANNWPLDVRVGLIGSCTNSSYEDMSRSASIVKDAAAHGLKSKTIFTVTPG 416
>sp|P39533|ACON2_YEAST Putative aconitase in PRP21-UBP12 intergenic region
Length = 789
Score = 290 bits (741), Expect = 4e-78
Identities = 133/239 (55%), Positives = 178/239 (74%), Gaps = 2/239 (0%)
Frame = +2
Query: 5 PGVLLIGTDSHTPNXXXXXXXXXXXXXADAVDVMADIPWELKCPRVIGVRLTGRLSGWTS 184
PG++++GTDSHTPN ADAVD + PWELK P+++GV+LTG+L+GW++
Sbjct: 181 PGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALTGTPWELKAPKILGVKLTGKLNGWST 240
Query: 185 SKDVILKVADILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPFNERM 364
KDVI K+A +LTV+GGTG IVEYFGEGV ++SCTGM TICNMGAEIGATTS FP+ E
Sbjct: 241 PKDVITKLAGLLTVRGGTGYIVEYFGEGVSTLSCTGMATICNMGAEIGATTSTFPYQEAH 300
Query: 365 MEYLVATNRADI--GAEAARYKEALLTADRNCKYDQVIEIDLSTLQPHINGPFTPDLAHP 538
YL ATNRA++ A+ A K L AD++ +YD+VIEIDLS ++PH+NGPFTPDL+ P
Sbjct: 301 KRYLQATNRAEVAEAADVALNKFNFLRADKDAQYDKVIEIDLSAIEPHVNGPFTPDLSTP 360
Query: 539 IAEMGKRAKQNGWPERISVGLIGSCTNSSYEDMTRAASIAKQAVDRGLRSKCAFTITPG 715
I++ +++ + WP+++S GLIGSCTNSSY+DM+R + KQA GL+ + F +TPG
Sbjct: 361 ISQYAEKSLKENWPQKVSAGLIGSCTNSSYQDMSRVVDLVKQASKAGLKPRIPFFVTPG 419
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,337,075
Number of Sequences: 369166
Number of extensions: 1182524
Number of successful extensions: 5349
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4824
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5103
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6462248555
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)