Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_K07
(528 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P21223|OMP7_STAAU 65 kDa outer membrane protein precurso... 33 0.42
sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amyla... 32 0.72
sp|P24764|VA39_VACCV Protein A39 32 0.95
sp|Q5HEI2|OMP7_STAAC 77 kDa outer membrane protein precursor 32 1.2
sp|Q24134|NELFD_DROME Negative elongation factor D 31 1.6
sp|P21062|VA39_VACCC Protein A39 31 1.6
sp|Q02001|TRPE_LACLA Anthranilate synthase component I 31 1.6
sp|Q11068|MGAT1_CAEEL Putative alpha-1,3-mannosyl-glycoprot... 31 1.6
sp|P70478|APC_RAT Adenomatous polyposis coli protein (APC p... 30 2.8
sp|Q9PA89|RS7_XYLFA 30S ribosomal protein S7 30 2.8
>sp|P21223|OMP7_STAAU 65 kDa outer membrane protein precursor (map-ND2C)
Length = 584
Score = 33.1 bits (74), Expect = 0.42
Identities = 33/137 (24%), Positives = 55/137 (40%), Gaps = 1/137 (0%)
Frame = +1
Query: 25 VRVNNDTKETFSSFDVNFNKKTNIKSLKFAVMERLSPSEDIKALSYNGRTYEGFEDLPID 204
+ VN ++ SS N N+ + K L+ V L I + +
Sbjct: 55 ITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRLSKQAKYTV---- 110
Query: 205 YMKNGDTLSLLIDQKLKVDVYTNGDNKTKPYRVECSYMDTSEELSDRVKDRLPYGT-NDI 381
Y KNG +ID LKV +YT T + +DT +++ D+ KD+ Y I
Sbjct: 111 YFKNGT--KKVID--LKVGIYTADLINTSEIKAININVDTKKQVEDKKKDKANYQVPYTI 166
Query: 382 VLTSDGSNLLNGVSLAK 432
+ N+L+ ++ K
Sbjct: 167 TVNGTSQNILSNLTFNK 183
>sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pullulanase) (Pullulanase
type II) [Includes: Alpha-amylase (1,4-alpha-D-glucan
glucanohydrolase); Pullulanase (1,4-alpha-D-glucan
glucanohydrolase) (Alpha-dextrin
endo-1,6-alpha-glucosidase)]
Length = 1861
Score = 32.3 bits (72), Expect = 0.72
Identities = 34/136 (25%), Positives = 59/136 (43%), Gaps = 4/136 (2%)
Frame = +1
Query: 34 NNDTKETFSSFDVNFNKKTNIKSLKFAVMERLSPSEDIKALSYNGRTYEGFEDLPIDYMK 213
NN+T + S+ + T S+ + + S I ++ NG L +D K
Sbjct: 1458 NNNTSTSGSNSSSTGSGSTGSTSITSNISNTSNTSNTIGVITKNGNVIT----LTLDAGK 1513
Query: 214 NGDTLSLLIDQKLKVDVYTNGDNKTKPYRVECSYMDTSEELSDRVKDRLPYGTN-DIVLT 390
D + D+K+ D+ T G+ + K ++ +DTS + KD + N I LT
Sbjct: 1514 AKDLIVNSKDKKVVFDITTIGEGQQKVVQISKDILDTS---AANGKDIVIKSDNASIALT 1570
Query: 391 SDGSN---LLNGVSLA 429
D N + NGV+++
Sbjct: 1571 KDALNQNQIQNGVNVS 1586
>sp|P24764|VA39_VACCV Protein A39
Length = 441
Score = 32.0 bits (71), Expect = 0.95
Identities = 26/107 (24%), Positives = 49/107 (45%), Gaps = 5/107 (4%)
Frame = +1
Query: 139 EDIKALSYNGRTYEGFEDLPIDYMKNGDTLSLLIDQKLKV-----DVYTNGDNKTKPYRV 303
+D+ NG Y F + ++ K G T + I +KV D G N P
Sbjct: 71 DDVLYTGVNGAVYT-FSNNKLN--KTGLTNNNYITTSIKVEDADKDTLVCGTNNGNP--- 124
Query: 304 ECSYMDTSEELSDRVKDRLPYGTNDIVLTSDGSNLLNGVSLAKQGMK 444
+C +D S++ R + PY + + + S +L+ ++++K+G+K
Sbjct: 125 KCWKIDGSDDPKHRGRGYAPYQNSKVTIISHNGCVLSDINISKEGIK 171
>sp|Q5HEI2|OMP7_STAAC 77 kDa outer membrane protein precursor
Length = 684
Score = 31.6 bits (70), Expect = 1.2
Identities = 32/137 (23%), Positives = 54/137 (39%), Gaps = 1/137 (0%)
Frame = +1
Query: 25 VRVNNDTKETFSSFDVNFNKKTNIKSLKFAVMERLSPSEDIKALSYNGRTYEGFEDLPID 204
+ VN ++ SS N N+ + K L+ V L I + +
Sbjct: 55 ITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRLSKQAKYTV---- 110
Query: 205 YMKNGDTLSLLIDQKLKVDVYTNGDNKTKPYRVECSYMDTSEELSDRVKDRLPYGT-NDI 381
Y KNG +ID LK +YT T + +DT +++ D+ KD+ Y I
Sbjct: 111 YFKNGT--KKVID--LKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANYQVPYTI 166
Query: 382 VLTSDGSNLLNGVSLAK 432
+ N+L+ ++ K
Sbjct: 167 TVNGTSQNILSNLTFNK 183
>sp|Q24134|NELFD_DROME Negative elongation factor D
Length = 578
Score = 31.2 bits (69), Expect = 1.6
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +1
Query: 169 RTYEGFEDLPIDYMKNGDTLSLLIDQKLKVDVYTNGDNKTK 291
R Y G + PID ++N L LL+D + V N ++K K
Sbjct: 302 RNYSGSDPPPIDLIRNPQFLELLVDALFRSGVKINPEHKPK 342
>sp|P21062|VA39_VACCC Protein A39
Length = 403
Score = 31.2 bits (69), Expect = 1.6
Identities = 26/107 (24%), Positives = 49/107 (45%), Gaps = 5/107 (4%)
Frame = +1
Query: 139 EDIKALSYNGRTYEGFEDLPIDYMKNGDTLSLLIDQKLKV-----DVYTNGDNKTKPYRV 303
+D+ NG Y F + ++ K G T + I +KV D G N P
Sbjct: 33 DDVLYTGVNGAVYT-FSNNKLN--KTGLTNNNYITTSIKVEDADKDTLVCGTNNGNP--- 86
Query: 304 ECSYMDTSEELSDRVKDRLPYGTNDIVLTSDGSNLLNGVSLAKQGMK 444
+C +D S++ R + PY + + + S +L+ ++++K+G+K
Sbjct: 87 KCWKIDGSDDPKHRGRGYAPYQNSKVTIISYNECVLSDINISKEGIK 133
>sp|Q02001|TRPE_LACLA Anthranilate synthase component I
Length = 456
Score = 31.2 bits (69), Expect = 1.6
Identities = 26/108 (24%), Positives = 48/108 (44%), Gaps = 5/108 (4%)
Frame = +1
Query: 10 RHEEIVRVNNDTKETFSSFDVNFNKKTNIKSLKFAVMERLSPSEDIKALSYNGRTYEGFE 189
+ E+++ + ++T S ++ NI+SL +E K + + E FE
Sbjct: 139 KREKLIFIEDNTYSQRSEKELQNALSANIESLSLLTEAENELTELSKLNFVSNMSQELFE 198
Query: 190 DLPI---DYMKNGDTLSLLIDQKLKVDVYTNGDNKTKPYRVE--CSYM 318
+ + ++NGD +++ Q+L D N N + RVE SYM
Sbjct: 199 EKVAKAKELIRNGDMFQVVLSQRLTADFTDNPFNYYRKLRVENPSSYM 246
>sp|Q11068|MGAT1_CAEEL Putative alpha-1,3-mannosyl-glycoprotein
2-beta-N-acetylglucosaminyltransferase
(N-glycosyl-oligosaccharide-glycoprotein
N-acetylglucosaminyltransferase I) (GNT-I) (GlcNAc-T I)
Length = 449
Score = 31.2 bits (69), Expect = 1.6
Identities = 25/81 (30%), Positives = 36/81 (44%), Gaps = 7/81 (8%)
Frame = +1
Query: 37 NDTKETFSSFDV------NFNKKTNIKSLKFAVMERLSPSEDIKALSYN-GRTYEGFEDL 195
ND F D+ NF KKTN++ +K AV + S N G++ D
Sbjct: 327 NDKYINFGKIDLDYLLPANFAKKTNLEVMKEAVELSIDNVASFVLSSENKGKSVRVMYDG 386
Query: 196 PIDYMKNGDTLSLLIDQKLKV 258
IDY++ D L ++ D K V
Sbjct: 387 NIDYIRKADKLHIMHDFKAGV 407
>sp|P70478|APC_RAT Adenomatous polyposis coli protein (APC protein)
Length = 2842
Score = 30.4 bits (67), Expect = 2.8
Identities = 30/123 (24%), Positives = 54/123 (43%), Gaps = 9/123 (7%)
Frame = +1
Query: 4 RIRHEEIVRVNNDTKETFSSFDVNFNKKTNIKSLKFAVMERLSPSEDIKALSY---NGRT 174
R+R +VN +T+ETFS D +KK ++K+ + ++L +ED + +
Sbjct: 1786 RVRKNTDSKVNVNTEETFS--DNKDSKKQSLKNNPKDLNDKLPDNEDRVRGGFTFDSPHH 1843
Query: 175 YEGFEDLPIDYMKNGDTLSLLID------QKLKVDVYTNGDNKTKPYRVECSYMDTSEEL 336
Y E P + +N SL D + K ++ ++K +V C +S +
Sbjct: 1844 YAPIEGTPYCFSRNDSLSSLDFDDDDVDLSREKAELRKGKESKDSEAKVTCHTEPSSSQQ 1903
Query: 337 SDR 345
S R
Sbjct: 1904 SAR 1906
>sp|Q9PA89|RS7_XYLFA 30S ribosomal protein S7
Length = 155
Score = 30.4 bits (67), Expect = 2.8
Identities = 24/107 (22%), Positives = 48/107 (44%), Gaps = 3/107 (2%)
Frame = +1
Query: 46 KETFSSFDVNFNKKTNIKSLKFAVMERLSPSEDIKALSYNGRTYEGFEDLPIDY---MKN 216
K + + +V K +N L ++ +SP+ ++K+ G TY+ +P++ +
Sbjct: 41 KIVYGAMNVIGEKNSNAIELLQKALDNVSPAVEVKSRRVGGATYQ----VPVEVRASRRM 96
Query: 217 GDTLSLLIDQKLKVDVYTNGDNKTKPYRVECSYMDTSEELSDRVKDR 357
+ LID K G+N + P+++ +D SE +K R
Sbjct: 97 ALAMRWLIDSSRK-----RGEN-SMPHKLAAELLDASESRGGAIKKR 137
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,767,431
Number of Sequences: 369166
Number of extensions: 1073294
Number of successful extensions: 2837
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2793
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2833
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3551563800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)