Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_J19
(800 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O95922|TTLL1_HUMAN Tubulin tyrosine ligase-like protein 1 153 6e-37
sp|Q91V51|TTLL1_MOUSE Tubulin tyrosine ligase-like protein 1 152 8e-37
sp|Q9BWV7|TTLL2_HUMAN Tubulin tyrosine ligase-like protein ... 108 1e-23
sp|Q14679|TTLL4_HUMAN Tubulin tyrosine ligase-like protein 4 97 4e-20
sp|Q80UG8|TTLL4_MOUSE Tubulin tyrosine ligase-like protein 4 97 7e-20
sp|Q9Y4R7|TTLL3_HUMAN Tubulin tyrosine ligase-like protein ... 79 1e-14
sp|Q6ZT98|TTLL7_HUMAN Tubulin tyrosine ligase-like protein ... 78 2e-14
sp|P38584|TTL_BOVIN Tubulin--tyrosine ligase (TTL) 68 3e-11
sp|P38160|TTL_PIG Tubulin--tyrosine ligase (TTL) 66 1e-10
sp|Q8NG68|TTL_HUMAN Tubulin--tyrosine ligase (TTL) 66 1e-10
>sp|O95922|TTLL1_HUMAN Tubulin tyrosine ligase-like protein 1
Length = 423
Score = 153 bits (386), Expect = 6e-37
Identities = 81/189 (42%), Positives = 111/189 (58%), Gaps = 2/189 (1%)
Frame = +3
Query: 117 YNPLKVWLYRDGFARFSNTRFT--LNSIDDIFIHLTNVAIQKTAPDYDPEKGCKWSIIKL 290
Y PL+ ++Y+ GF RF ++T + +D++F+HLTNVAIQK DY+ G KW++ L
Sbjct: 206 YRPLRCYMYKLGFCRFCTVKYTPSTSELDNMFVHLTNVAIQKHGEDYNHIHGGKWTVSNL 265
Query: 291 RRYLISLHGIEKIENLFRSIDNIIITSLTSVRKVIINDKHCFELYGYDILLDADLRPWLL 470
R YL S G E LF I II+ SL +V V+ NDKHCFE YGYDI++D L+PWL+
Sbjct: 266 RLYLESTRGKEVTSKLFDEIHWIIVQSLKAVAPVMNNDKHCFECYGYDIIIDDKLKPWLI 325
Query: 471 EINAAPSLTASSKEDYRLKCELLDDVLNVIDMEGRLTGKEKRIGGFDLLWNDGPVNCMEV 650
E+NA+PSLT+S+ D LK L++D LN+ G + D WN P
Sbjct: 326 EVNASPSLTSSTANDRILKYNLINDTLNIAVPNGEIP---------DCKWNKSP------ 370
Query: 651 FPNMISNNY 677
P + NY
Sbjct: 371 -PKEVLGNY 378
Score = 30.8 bits (68), Expect = 4.5
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = +2
Query: 2 PYLINGRKFDLRIYALV 52
P LI GRKFDLR+Y LV
Sbjct: 187 PLLIGGRKFDLRLYVLV 203
>sp|Q91V51|TTLL1_MOUSE Tubulin tyrosine ligase-like protein 1
Length = 423
Score = 152 bits (385), Expect = 8e-37
Identities = 81/189 (42%), Positives = 111/189 (58%), Gaps = 2/189 (1%)
Frame = +3
Query: 117 YNPLKVWLYRDGFARFSNTRFT--LNSIDDIFIHLTNVAIQKTAPDYDPEKGCKWSIIKL 290
Y PL+ ++Y+ GF RF ++T + +D++F+HLTNVAIQK DY+ G KW++ L
Sbjct: 206 YRPLRCYMYKLGFCRFCTVKYTPSTSELDNMFVHLTNVAIQKHGEDYNHIHGGKWTVNNL 265
Query: 291 RRYLISLHGIEKIENLFRSIDNIIITSLTSVRKVIINDKHCFELYGYDILLDADLRPWLL 470
R YL S G E LF I II+ SL +V V+ NDKHCFE YGYDI++D L+PWL+
Sbjct: 266 RLYLESTRGREVTSKLFDEIHWIIVQSLKAVAPVMNNDKHCFECYGYDIIIDDKLKPWLI 325
Query: 471 EINAAPSLTASSKEDYRLKCELLDDVLNVIDMEGRLTGKEKRIGGFDLLWNDGPVNCMEV 650
E+NA+PSLT+S+ D LK L++D LN+ G + D WN P
Sbjct: 326 EVNASPSLTSSTANDRILKYNLINDTLNIAVPNGEIP---------DCKWNKSP------ 370
Query: 651 FPNMISNNY 677
P + NY
Sbjct: 371 -PKEVLGNY 378
Score = 30.8 bits (68), Expect = 4.5
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = +2
Query: 2 PYLINGRKFDLRIYALV 52
P LI GRKFDLR+Y LV
Sbjct: 187 PLLIGGRKFDLRLYVLV 203
>sp|Q9BWV7|TTLL2_HUMAN Tubulin tyrosine ligase-like protein 2 (Testis-specific protein
NYD-TSPG)
Length = 592
Score = 108 bits (271), Expect = 1e-23
Identities = 51/157 (32%), Positives = 98/157 (62%), Gaps = 5/157 (3%)
Frame = +3
Query: 117 YNPLKVWLYRDGFARFSNTRFTLNSIDDIFIHLTNVAIQKTAPDYDPEK-----GCKWSI 281
+ PL +++Y++G RF+ +F L+++ + + HLTN +I K+ Y+ K GCKW++
Sbjct: 265 FKPLTIYVYQEGLVRFATEKFDLSNLQNNYAHLTNSSINKSGASYEKIKEVIGHGCKWTL 324
Query: 282 IKLRRYLISLHGIEKIENLFRSIDNIIITSLTSVRKVIINDKHCFELYGYDILLDADLRP 461
+ YL S + + L++ I ++I ++ ++ + +CFEL+G+DIL+D +L+P
Sbjct: 325 SRFFSYLRSWDVDDLL--LWKKIHRMVILTILAIAPSVPFAANCFELFGFDILIDDNLKP 382
Query: 462 WLLEINAAPSLTASSKEDYRLKCELLDDVLNVIDMEG 572
WLLE+N +P+LT D +K +L+ D++++I + G
Sbjct: 383 WLLEVNYSPALTLDCSTDVLVKRKLVHDIIDLIYLNG 419
>sp|Q14679|TTLL4_HUMAN Tubulin tyrosine ligase-like protein 4
Length = 1199
Score = 97.4 bits (241), Expect = 4e-20
Identities = 52/163 (31%), Positives = 101/163 (61%), Gaps = 16/163 (9%)
Frame = +3
Query: 117 YNPLKVWLYRDGFARFSNTRFT--LNSIDDIFIHLTNVAIQKTAPDYDPE------KGCK 272
Y+PL+++L+ DG RF++ +++ + S+ + F+HLTN ++ K +Y +G K
Sbjct: 774 YDPLRIYLFSDGLVRFASCKYSPSMKSLGNKFMHLTNYSVNKKNAEYQANADEMACQGHK 833
Query: 273 WSIIKLRRYLISLHGIEKIENLFRSIDNIIITSL-------TSVRKVIINDKH-CFELYG 428
W++ L YL S G+ + ++ I ++++ ++ TS+ K+ + + C EL+G
Sbjct: 834 WALKALWNYL-SQKGVNS-DAIWEKIKDVVVKTIISSEPYVTSLLKMYVRRPYSCHELFG 891
Query: 429 YDILLDADLRPWLLEINAAPSLTASSKEDYRLKCELLDDVLNV 557
+DI+LD +L+PW+LE+N +PSL +SS D +K +++ D+LN+
Sbjct: 892 FDIMLDENLKPWVLEVNISPSLHSSSPLDISIKGQMIRDLLNL 934
Score = 32.3 bits (72), Expect = 1.5
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +2
Query: 2 PYLINGRKFDLRIYALVVS 58
PYLI+G KFDLRIY V S
Sbjct: 755 PYLISGSKFDLRIYVYVTS 773
>sp|Q80UG8|TTLL4_MOUSE Tubulin tyrosine ligase-like protein 4
Length = 934
Score = 96.7 bits (239), Expect = 7e-20
Identities = 51/163 (31%), Positives = 97/163 (59%), Gaps = 16/163 (9%)
Frame = +3
Query: 117 YNPLKVWLYRDGFARFSNTRFT--LNSIDDIFIHLTNVAIQKTAPDYDPE------KGCK 272
Y+PL+++L+ DG RF++ +++ + S+ + F+HLTN ++ K +Y +G K
Sbjct: 510 YDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNTEYQANADETACQGHK 569
Query: 273 WSIIKLRRYLISLHGIEKIENLFRSIDNIIITSLTSVRKVIIN--------DKHCFELYG 428
W++ L YL S GI + ++ I ++++ ++ S + N C EL+G
Sbjct: 570 WALKALWNYL-SQKGINS-DAIWEKIKDVVVKTIISSEPYVTNLLKLYVRRPYSCHELFG 627
Query: 429 YDILLDADLRPWLLEINAAPSLTASSKEDYRLKCELLDDVLNV 557
+DI+LD +L+PW+LE+N +PSL ++S D +K +++ D+LN+
Sbjct: 628 FDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNL 670
Score = 32.3 bits (72), Expect = 1.5
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +2
Query: 2 PYLINGRKFDLRIYALVVS 58
PYLI+G KFDLRIY V S
Sbjct: 491 PYLISGSKFDLRIYVYVTS 509
>sp|Q9Y4R7|TTLL3_HUMAN Tubulin tyrosine ligase-like protein 3 (HOTTL)
Length = 352
Score = 79.3 bits (194), Expect = 1e-14
Identities = 53/176 (30%), Positives = 88/176 (50%), Gaps = 8/176 (4%)
Frame = +3
Query: 117 YNPLKVWLYRDGFARFSNTRFTLNSIDDIFIHLTNVAIQKTA-------PDYDPEKGCKW 275
+NPL VW YRD + RFS F+L ++D+ +HL N +IQK P P+ W
Sbjct: 134 WNPLTVWFYRDSYIRFSTQPFSLKNLDNS-VHLCNNSIQKHLENSCHRHPLLPPDN--MW 190
Query: 276 SIIKLRRYLISLHGIEKIENLF-RSIDNIIITSLTSVRKVIINDKHCFELYGYDILLDAD 452
S + + +L + + + + +I +L + + + K FELYG D + D
Sbjct: 191 SSQRFQAHLQEMGAPNAWSTIIVPGMKDAVIHALQTSQDTVQCRKASFELYGADFVFGED 250
Query: 453 LRPWLLEINAAPSLTASSKEDYRLKCELLDDVLNVIDMEGRLTGKEKRIGGFDLLW 620
+PWL+EINA+P++ S+ RL + D L V+ R+ + G F+L++
Sbjct: 251 FQPWLIEINASPTMAPSTAVTARLCAGVQADTLRVV--IDRMLDRNCDTGAFELIY 304
>sp|Q6ZT98|TTLL7_HUMAN Tubulin tyrosine ligase-like protein 7 (Protein NYD-SP30)
Length = 887
Score = 78.2 bits (191), Expect = 2e-14
Identities = 49/170 (28%), Positives = 87/170 (51%), Gaps = 21/170 (12%)
Frame = +3
Query: 120 NPLKVWLYRDGFARFSNTRF---TLNSIDDIFIHLTNVAIQKTAPDYDPE----KGCKWS 278
+PLK++LY DG R ++ +++ +++HLTN ++ K ++ + KG K S
Sbjct: 214 DPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNEHFERDETENKGSKRS 273
Query: 279 IIKLRRYL-ISLHGIEKIENLFRSIDNIIITSLTSVRKVIIN-------------DKHCF 416
I +L + H + K + I +++ +L +++ + CF
Sbjct: 274 IKWFTEFLQANQHDVAKF---WSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCF 330
Query: 417 ELYGYDILLDADLRPWLLEINAAPSLTASSKEDYRLKCELLDDVLNVIDM 566
E+ G+DILLD L+PWLLEIN APS K DY +K +L + L ++++
Sbjct: 331 EVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNI 380
Score = 30.4 bits (67), Expect = 5.9
Identities = 13/19 (68%), Positives = 15/19 (78%)
Frame = +2
Query: 2 PYLINGRKFDLRIYALVVS 58
P+L+ G KFDLRIY LV S
Sbjct: 194 PFLMEGYKFDLRIYILVTS 212
>sp|P38584|TTL_BOVIN Tubulin--tyrosine ligase (TTL)
Length = 377
Score = 67.8 bits (164), Expect = 3e-11
Identities = 46/145 (31%), Positives = 79/145 (54%), Gaps = 6/145 (4%)
Frame = +3
Query: 132 VWLYRDGFARFSNTRFTLNSIDDIFIHLTNVAIQKT-APDYDP-EKGCKWSIIKLRRYLI 305
++LYR+G R ++ + +++ D HLTN IQK + +Y E+G + RYL
Sbjct: 213 IYLYREGVLRTASEPYHMDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFEAFNRYLT 272
Query: 306 SLHGIEKIENLFRSIDNIIITSLTSVRKVIINDKHC----FELYGYDILLDADLRPWLLE 473
S I ++ I +II + L SV I + KH F+L+G+D ++D +L+ WL+E
Sbjct: 273 SALNITLESSILLQIKHIIRSCLMSVEPAI-STKHLPYQSFQLFGFDFMVDEELKVWLIE 331
Query: 474 INAAPSLTASSKEDYRLKCELLDDV 548
+N AP A +++ Y C+ + D+
Sbjct: 332 VNGAP---ACAQKLYAELCQGIVDI 353
>sp|P38160|TTL_PIG Tubulin--tyrosine ligase (TTL)
Length = 379
Score = 66.2 bits (160), Expect = 1e-10
Identities = 45/145 (31%), Positives = 79/145 (54%), Gaps = 6/145 (4%)
Frame = +3
Query: 132 VWLYRDGFARFSNTRFTLNSIDDIFIHLTNVAIQKT-APDYDP-EKGCKWSIIKLRRYLI 305
++LYR+G R ++ + ++ D HLTN IQK + +Y E+G + + +YL
Sbjct: 213 IYLYREGVLRTASEPYHTDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFEEFNQYLT 272
Query: 306 SLHGIEKIENLFRSIDNIIITSLTSVRKVIINDKHC----FELYGYDILLDADLRPWLLE 473
S I ++ I +II + L SV I + +H F+L+G+D ++D DL+ WL+E
Sbjct: 273 SALNITLESSILLQIKHIIRSCLLSVEPAI-STRHLPYQSFQLFGFDFMVDEDLKVWLIE 331
Query: 474 INAAPSLTASSKEDYRLKCELLDDV 548
+N AP A +++ Y C+ + D+
Sbjct: 332 VNGAP---ACAQKLYAELCQGIVDI 353
>sp|Q8NG68|TTL_HUMAN Tubulin--tyrosine ligase (TTL)
Length = 377
Score = 66.2 bits (160), Expect = 1e-10
Identities = 45/145 (31%), Positives = 79/145 (54%), Gaps = 6/145 (4%)
Frame = +3
Query: 132 VWLYRDGFARFSNTRFTLNSIDDIFIHLTNVAIQKT-APDYDP-EKGCKWSIIKLRRYLI 305
++LYR+G R ++ + +++ D HLTN IQK + +Y E+G + + +YL
Sbjct: 213 IYLYREGVLRTASEPYHVDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFKEFNQYLT 272
Query: 306 SLHGIEKIENLFRSIDNIIITSLTSVRKVIINDKHC----FELYGYDILLDADLRPWLLE 473
S I ++ I +II L SV I + KH F+L+G+D ++D +L+ WL+E
Sbjct: 273 SALNITLESSILLQIKHIIRNCLLSVEPAI-STKHLPYQSFQLFGFDFMVDEELKVWLIE 331
Query: 474 INAAPSLTASSKEDYRLKCELLDDV 548
+N AP A +++ Y C+ + D+
Sbjct: 332 VNGAP---ACAQKLYAELCQGIVDI 353
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,718,075
Number of Sequences: 369166
Number of extensions: 1762086
Number of successful extensions: 4414
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4265
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4398
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7570361805
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)