Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_I20
(841 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9VQG2|APH1_DROME Gamma-secretase subunit Aph-1 (Preseni... 64 4e-10
sp|Q8JHE9|APH1B_BRARE Gamma-secretase subunit Aph-1b (Anter... 44 6e-04
sp|Q96BI3|APH1A_HUMAN Gamma-secretase subunit APH-1A (APH-1... 40 0.008
sp|Q8C7N7|APH1B_MOUSE Gamma-secretase subunit APH-1B (APH-1... 39 0.014
sp|Q9DCZ9|APH1C_MOUSE Putative gamma-secretase subunit APH-1C 39 0.014
sp|Q8WW43|APH1B_HUMAN Gamma-secretase subunit APH-1B (APH-1... 39 0.014
sp|Q8BVF7|APH1A_MOUSE Gamma-secretase subunit APH-1A (APH-1... 39 0.014
sp|Q5RDM3|APH1B_PONPY Gamma-secretase subunit APH-1B (APH-1... 39 0.014
sp|P32832|NPL6_YEAST Protein NPL6 32 1.7
sp|P07270|PHO4_YEAST Phosphate system positive regulatory p... 32 2.9
>sp|Q9VQG2|APH1_DROME Gamma-secretase subunit Aph-1 (Presenilin-stabilization factor)
Length = 238
Score = 64.3 bits (155), Expect = 4e-10
Identities = 53/198 (26%), Positives = 75/198 (37%)
Frame = +3
Query: 42 MACDELIGCLLTSFGIPIVLVLFTIITHPLRVITXXXXXXXXXXXXXXXXXXXYAVVPLK 221
M E GC +FG P L +FTI P+R+I YA++PLK
Sbjct: 1 MTLPEFFGCTFIAFGPPFALFVFTIANDPVRIIILIAAAFFWLLSLLISSLW-YALIPLK 59
Query: 222 SKLAFGLVFSVIFQEAIRFLFIYCLHKIDRGIKLIMFDLKSSEPSTDRSLTSNIDIATTS 401
LAFG+VFSV FQEA R++ L ++G+ + D + ++ + S + S
Sbjct: 60 EFLAFGVVFSVCFQEAFRYIIYRILRSTEQGLHAVAEDTRVTDNKHILAYVSGLGFGIIS 119
Query: 402 NNINYHRDGEEGNKPLIASDKSTEDIFNVTMFAYVXXXXXXXXXXXXXXXNMLVALRGPG 581
MFA V N+L + GPG
Sbjct: 120 G-----------------------------MFALV---------------NVLADMSGPG 135
Query: 582 TLGYFGQSADFIDFYAAQ 635
T+G G + F AAQ
Sbjct: 136 TMGLKGGTELFFVTSAAQ 153
>sp|Q8JHE9|APH1B_BRARE Gamma-secretase subunit Aph-1b (Anterior-pharynx-defective protein
1b)
Length = 258
Score = 43.9 bits (102), Expect = 6e-04
Identities = 29/94 (30%), Positives = 40/94 (42%), Gaps = 9/94 (9%)
Frame = +3
Query: 63 GCLLTSFGIPIVLVLFTIITHPLRVITXXXXXXXXXXXXXXXXXXXYAVVPLKSK----- 227
GC +FG I L +FTI PLRVI + V + +K
Sbjct: 8 GCTFIAFGPAIALFMFTIARDPLRVIFLIAGAFFWLVSLLLSSLVWFITVQISNKNSATQ 67
Query: 228 ----LAFGLVFSVIFQEAIRFLFIYCLHKIDRGI 317
L FG+V SV+ QEA R+ + L K + G+
Sbjct: 68 QRGLLIFGVVLSVLLQEAFRYGYYRLLKKANEGL 101
>sp|Q96BI3|APH1A_HUMAN Gamma-secretase subunit APH-1A (APH-1a) (Aph-1alpha)
(Presenilin-stabilization factor)
Length = 265
Score = 40.0 bits (92), Expect = 0.008
Identities = 29/110 (26%), Positives = 40/110 (36%), Gaps = 9/110 (8%)
Frame = +3
Query: 42 MACDELIGCLLTSFGIPIVLVLFTIITHPLRVITXXXXXXXXXXXXXXXXXXXYAVVPLK 221
M GC +FG L L T+ PLRVI + +V +
Sbjct: 1 MGAAVFFGCTFVAFGPAFALFLITVAGDPLRVIILVAGAFFWLVSLLLASVVWFILVHVT 60
Query: 222 SK---------LAFGLVFSVIFQEAIRFLFIYCLHKIDRGIKLIMFDLKS 344
+ L FG SV+ QE RF + L K D G+ + D +S
Sbjct: 61 DRSDARLQYGLLIFGAAVSVLLQEVFRFAYYKLLKKADEGLASLSEDGRS 110
>sp|Q8C7N7|APH1B_MOUSE Gamma-secretase subunit APH-1B (APH-1b) (Aph-1beta)
Length = 257
Score = 39.3 bits (90), Expect = 0.014
Identities = 29/104 (27%), Positives = 38/104 (36%), Gaps = 9/104 (8%)
Frame = +3
Query: 42 MACDELIGCLLTSFGIPIVLVLFTIITHPLRVITXXXXXXXXXXXXXXXXXXXYAVVPLK 221
M GC +FG + L +FTI T PLRVI + V +
Sbjct: 1 MTAAVFFGCAFIAFGPALALYVFTIATDPLRVIFLIAGAFFWLVSLLLSSVFWFLVRVIT 60
Query: 222 SK---------LAFGLVFSVIFQEAIRFLFIYCLHKIDRGIKLI 326
L FG++ SV QE R + L K G+K I
Sbjct: 61 DNRDGPVQNYLLIFGVLLSVCIQELFRLAYYKLLKKASEGLKSI 104
>sp|Q9DCZ9|APH1C_MOUSE Putative gamma-secretase subunit APH-1C
Length = 258
Score = 39.3 bits (90), Expect = 0.014
Identities = 30/104 (28%), Positives = 37/104 (35%), Gaps = 9/104 (8%)
Frame = +3
Query: 42 MACDELIGCLLTSFGIPIVLVLFTIITHPLRVITXXXXXXXXXXXXXXXXXXXYAVVPLK 221
M GC +FG L LFTI T PLRVI + V +
Sbjct: 1 MTLPVFFGCAFIAFGPAFALYLFTIATDPLRVIFLIAGAFFWLVSLLLSSMFWFLVRVIT 60
Query: 222 SK---------LAFGLVFSVIFQEAIRFLFIYCLHKIDRGIKLI 326
+ L FG + SV QE R + L K G+K I
Sbjct: 61 NNRDESVQNYLLIFGALLSVCIQELFRLAYYKLLKKASEGLKSI 104
>sp|Q8WW43|APH1B_HUMAN Gamma-secretase subunit APH-1B (APH-1b) (Aph-1beta)
(Presenilin-stabilization factor-like)
Length = 257
Score = 39.3 bits (90), Expect = 0.014
Identities = 31/104 (29%), Positives = 40/104 (38%), Gaps = 9/104 (8%)
Frame = +3
Query: 42 MACDELIGCLLTSFGIPIVLVLFTIITHPLRVITXXXXXXXXXXXXXXXXXXXY-AVVPL 218
M GC +FG + L +FTI T PLR+I + A V +
Sbjct: 1 MTAAVFFGCAFIAFGPALALYVFTIATEPLRIIFLIAGAFFWLVSLLISSLVWFMARVII 60
Query: 219 KSK--------LAFGLVFSVIFQEAIRFLFIYCLHKIDRGIKLI 326
+K L FG SV QE RF + L K G+K I
Sbjct: 61 DNKDGPTQKYLLIFGAFVSVYIQEMFRFAYYKLLKKASEGLKSI 104
>sp|Q8BVF7|APH1A_MOUSE Gamma-secretase subunit APH-1A (APH-1a) (Aph-1alpha)
Length = 265
Score = 39.3 bits (90), Expect = 0.014
Identities = 29/110 (26%), Positives = 40/110 (36%), Gaps = 9/110 (8%)
Frame = +3
Query: 42 MACDELIGCLLTSFGIPIVLVLFTIITHPLRVITXXXXXXXXXXXXXXXXXXXYAVVPLK 221
M GC +FG L L T+ PLRVI + +V +
Sbjct: 1 MGAAVFFGCTFVAFGPAFSLFLITVAGDPLRVIILVAGAFFWLVSLLLASVVWFILVHVT 60
Query: 222 SK---------LAFGLVFSVIFQEAIRFLFIYCLHKIDRGIKLIMFDLKS 344
+ L FG SV+ QE RF + L K D G+ + D +S
Sbjct: 61 DRSDARLQYGLLIFGAAVSVLLQEVFRFAYYKLLKKADEGLASLSEDGRS 110
>sp|Q5RDM3|APH1B_PONPY Gamma-secretase subunit APH-1B (APH-1b) (Aph-1beta)
Length = 257
Score = 39.3 bits (90), Expect = 0.014
Identities = 31/104 (29%), Positives = 40/104 (38%), Gaps = 9/104 (8%)
Frame = +3
Query: 42 MACDELIGCLLTSFGIPIVLVLFTIITHPLRVITXXXXXXXXXXXXXXXXXXXY-AVVPL 218
M GC +FG + L +FTI T PLR+I + A V +
Sbjct: 1 MTAAVFFGCAFIAFGPALALYVFTIATEPLRIIFLIAGAFFWLVSLLISSLVWFMARVII 60
Query: 219 KSK--------LAFGLVFSVIFQEAIRFLFIYCLHKIDRGIKLI 326
+K L FG SV QE RF + L K G+K I
Sbjct: 61 DNKDGPTQKYLLIFGTFVSVYIQEMFRFAYYRLLKKASEGLKSI 104
>sp|P32832|NPL6_YEAST Protein NPL6
Length = 435
Score = 32.3 bits (72), Expect = 1.7
Identities = 23/80 (28%), Positives = 37/80 (46%)
Frame = -3
Query: 779 EGELLASVKHE*SM*TSRMAPNFTRDLAWYALGHEHVERDVEQHDEQRLGGVKIDEISRL 600
EG L + V+HE ++ PN+ D + D + +E+ GV +EIS
Sbjct: 5 EGGLASEVEHEKRSRSTSNRPNYAIDTEDLDIDENDENEDDDYREEEANEGVNEEEISDE 64
Query: 599 AEISERSGASQRDQHVQRGE 540
E +SG ++R +HV E
Sbjct: 65 EEQINKSGRNKR-RHVDEEE 83
>sp|P07270|PHO4_YEAST Phosphate system positive regulatory protein PHO4
Length = 312
Score = 31.6 bits (70), Expect = 2.9
Identities = 18/47 (38%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
Frame = -1
Query: 622 KSMKSADWPKYPSVPGPRSATSMFR--EANKPDIKPNPRPPTYANIV 488
KS S K PGP SATS+F ++ P KP P+P Y ++
Sbjct: 148 KSNSSPYLNKRRGKPGPDSATSLFELPDSVIPTPKPKPKPKQYPKVI 194
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,581,375
Number of Sequences: 369166
Number of extensions: 1693948
Number of successful extensions: 5262
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5013
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5252
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8197207050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)