Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_G19
(655 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P32369|BAIG_EUBSP Bile acid transporter 51 2e-06
sp|P37643|YHJE_ECOLI Inner membrane metabolite transport pr... 48 2e-05
sp|P46333|CSBC_BACSU Probable metabolite transport protein ... 47 6e-05
sp|P47234|LACY_CITFR Lactose permease (Lactose-proton symport) 42 0.002
sp|Q92EE1|LMRB_LISIN Lincomycin resistance protein lmrB 42 0.002
sp|Q8Y9K8|LMRB_LISMO Lincomycin resistance protein lmrB 42 0.002
sp|Q58955|YF60_METJA Putative transporter MJ1560 41 0.002
sp|Q08299|ENB1_YEAST Siderophore iron transporter ENB1 (Sid... 39 0.012
sp|P46907|NARK_BACSU Nitrite extrusion protein (Nitrite fac... 38 0.020
sp|Q8Z4Z9|YFCJ_SALTI Hypothetical UPF0226 protein yfcJ 38 0.026
>sp|P32369|BAIG_EUBSP Bile acid transporter
Length = 477
Score = 51.2 bits (121), Expect = 2e-06
Identities = 33/144 (22%), Positives = 67/144 (46%), Gaps = 1/144 (0%)
Frame = +1
Query: 1 GLNGADAALITSLLYIVSAACSPVMGFLIDLTGRNLFWVLTGTLATLGS-HALYAFTFAN 177
G++ + I+ L + SAAC+P++G L D+ GR +L + G+ A +
Sbjct: 42 GIDYNNTTWISLALAMSSAACAPILGKLGDVLGRRTTLLLGIVIFAAGNVLTAVATSLIF 101
Query: 178 PLGVMIFLGVSYSVLASSLWPMVALVIPAHQRGTAFGIMQSVQNLGLAVVAIIAGLIQDK 357
L +G+ + ++ + + P + G AFG+ + + + V GLI +
Sbjct: 102 MLAARFIVGIGTAAISPIVMAYIVTEYPQEETGKAFGLYMLISSGAVVVGPTCGGLIMNA 161
Query: 358 YGFLILEVFFSVWLCITLVLIVLL 429
G+ ++ +W+C+ L ++V L
Sbjct: 162 AGWRVM-----MWVCVALCVVVFL 180
>sp|P37643|YHJE_ECOLI Inner membrane metabolite transport protein yhjE
Length = 440
Score = 48.1 bits (113), Expect = 2e-05
Identities = 40/130 (30%), Positives = 61/130 (46%), Gaps = 8/130 (6%)
Frame = +1
Query: 67 PVMGFLIDLTGRNLFWVLTGTLATLGSHALYAFT----FANPLGVMIFLGVSYSVLASSL 234
PV G L D GR V+ TL L AL+AF NP+ V FL + S++ +
Sbjct: 306 PVAGLLADAFGRRKSMVIITTLIIL--FALFAFNPLLGSGNPILVFAFLLLGLSLMGLTF 363
Query: 235 WPMVALV---IPAHQRGTAFGIMQSVQN-LGLAVVAIIAGLIQDKYGFLILEVFFSVWLC 402
PM AL+ P R T +V + LG +V IA +Q YG + ++ +
Sbjct: 364 GPMGALLPELFPTEVRYTGASFSYNVASILGASVAPYIAAWLQTNYGLGAVGLYLAAMAG 423
Query: 403 ITLVLIVLLY 432
+TL+ ++L +
Sbjct: 424 LTLIALLLTH 433
>sp|P46333|CSBC_BACSU Probable metabolite transport protein csbC
Length = 461
Score = 46.6 bits (109), Expect = 6e-05
Identities = 52/195 (26%), Positives = 83/195 (42%), Gaps = 17/195 (8%)
Frame = +1
Query: 1 GLNGADAALITSLLYIVSAACSPVMGFLIDLTGRN--LFW----------VLTGTLATLG 144
GL + +AL T + I++ LID GR L W L+G L TLG
Sbjct: 273 GLGTSASALGTMGIGILNVIMCITAMILIDRVGRKKLLIWGSVGITLSLAALSGVLLTLG 332
Query: 145 SHALYAFTFANPLGVMIFLGVSYSVLASSLWPMVALVI-----PAHQRGTAFGIMQSVQN 309
A A+ ++FLGV Y V + W V V+ P+ RG A G V +
Sbjct: 333 LSASTAWM------TVVFLGV-YIVFYQATWGPVVWVLMPELFPSKARGAATGFTTLVLS 385
Query: 310 LGLAVVAIIAGLIQDKYGFLILEVFFSVWLCITLVLIVLLYLWDVQNGGKLNESGPSRRK 489
+V+++ L+ G + + FSV +C+ L Y+ G L E S +K
Sbjct: 386 AANLIVSLVFPLMLSAMGIAWVFMVFSV-ICL-LSFFFAFYMVPETKGKSLEEIEASLKK 443
Query: 490 KLKKSEYNVSETMDK 534
+ KK + ++ +++
Sbjct: 444 RFKKKKSTQNQVLNE 458
>sp|P47234|LACY_CITFR Lactose permease (Lactose-proton symport)
Length = 416
Score = 41.6 bits (96), Expect = 0.002
Identities = 41/170 (24%), Positives = 74/170 (43%), Gaps = 14/170 (8%)
Frame = +1
Query: 4 LNGADAALITSLLYIVSAACSPVMGFLIDLTG--RNLFWVLTGTLATLGSHALYAFTFAN 177
++ D +I + + + S P+ G L D G ++L WV+TG L +Y F
Sbjct: 40 ISKGDTGIIFACISLFSLLFQPIFGLLSDKLGLRKHLLWVITGMLVMFAPFFIYVFGPLL 99
Query: 178 PLGVM-------IFLGVSYSVLASSLWPMVALVIPAHQRGT-AFGIMQSVQNLGLAVVAI 333
+ ++ I+LG Y+ A ++ A + A +R FG + +G A+ A
Sbjct: 100 QVNILLGSIVGGIYLGFIYNAGAPAI---EAYIEKASRRSNFEFGRARMFGCVGWALCAS 156
Query: 334 IAGLIQDKYGFLILEVFFSVWL----CITLVLIVLLYLWDVQNGGKLNES 471
IAG++ + F WL + L L++L DV + K+ ++
Sbjct: 157 IAGIM------FTINNQFVFWLGSGCAVILALLLLFSKTDVPSSAKVADA 200
>sp|Q92EE1|LMRB_LISIN Lincomycin resistance protein lmrB
Length = 471
Score = 41.6 bits (96), Expect = 0.002
Identities = 34/128 (26%), Positives = 61/128 (47%), Gaps = 8/128 (6%)
Frame = +1
Query: 73 MGFLIDLTGRNLFWVLT-GTLATLGSHALYAFTFA--NPLGVMIFLG-----VSYSVLAS 228
+G L+ ++G L W T G T S ++ A +P M+ +G V ++L
Sbjct: 63 LGILVPISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLP 122
Query: 229 SLWPMVALVIPAHQRGTAFGIMQSVQNLGLAVVAIIAGLIQDKYGFLILEVFFSVWLCIT 408
++ + L+ P H+RG+A G++ V AV I+GLI + L + W+ +
Sbjct: 123 LMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILEN-----LTWNWIFWISLP 177
Query: 409 LVLIVLLY 432
++I LL+
Sbjct: 178 FLIIALLF 185
>sp|Q8Y9K8|LMRB_LISMO Lincomycin resistance protein lmrB
Length = 471
Score = 41.6 bits (96), Expect = 0.002
Identities = 34/128 (26%), Positives = 61/128 (47%), Gaps = 8/128 (6%)
Frame = +1
Query: 73 MGFLIDLTGRNLFWVLT-GTLATLGSHALYAFTFA--NPLGVMIFLG-----VSYSVLAS 228
+G L+ ++G L W T G T S ++ A +P M+ +G V ++L
Sbjct: 63 LGILVPISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLP 122
Query: 229 SLWPMVALVIPAHQRGTAFGIMQSVQNLGLAVVAIIAGLIQDKYGFLILEVFFSVWLCIT 408
++ + L+ P H+RG+A G++ V AV I+GLI + L + W+ +
Sbjct: 123 LMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILEN-----LTWNWIFWISLP 177
Query: 409 LVLIVLLY 432
++I LL+
Sbjct: 178 FLIIALLF 185
>sp|Q58955|YF60_METJA Putative transporter MJ1560
Length = 386
Score = 41.2 bits (95), Expect = 0.002
Identities = 31/142 (21%), Positives = 67/142 (47%), Gaps = 1/142 (0%)
Frame = +1
Query: 13 ADAALITSLLYIVSAACSPVMGFLIDLTGRNLFWVLTGTLATLGSHALY-AFTFANPLGV 189
+ + +L I+ A G L D G N+ ++ + + G + L + TF L
Sbjct: 245 SQVGFMIALTNILMALLQRSFGKLYDKLG-NIMIIIGIFIISFGMYLLSTSTTFLTILAS 303
Query: 190 MIFLGVSYSVLASSLWPMVALVIPAHQRGTAFGIMQSVQNLGLAVVAIIAGLIQDKYGFL 369
+ + V S+ +++ + IP H++G A G+ + N+G+ + A+ G + D G
Sbjct: 304 LTIIAVGSSISSTATTSLAVKDIPTHRKGEAMGLFTTSINIGMFIGAVSFGFLADILGIA 363
Query: 370 ILEVFFSVWLCITLVLIVLLYL 435
+ F +++ ++V+ ++ YL
Sbjct: 364 NMYKFSAIF---SIVVGIISYL 382
>sp|Q08299|ENB1_YEAST Siderophore iron transporter ENB1 (Siderophore iron transporter
ARN4) (Enterobactin permease)
Length = 606
Score = 38.9 bits (89), Expect = 0.012
Identities = 29/122 (23%), Positives = 55/122 (45%), Gaps = 6/122 (4%)
Frame = +1
Query: 1 GLNGADAALITSLLYIVSAACSPVMGFLIDLTGRNLFWVLTGTL-ATLGSHALYAFT--- 168
G + A I L + + + V+G ++ +V+ G+L +G LY +
Sbjct: 379 GESTLSAQRIAQLYNFLQSCTNIVVGIMLHFYPHPKVFVVAGSLLGVIGMGLLYKYRVVY 438
Query: 169 --FANPLGVMIFLGVSYSVLASSLWPMVALVIPAHQRGTAFGIMQSVQNLGLAVVAIIAG 342
+ +G I +G++ ++ +W +V ++ T G++ SV +G AV A IAG
Sbjct: 439 DGISGLIGAEIVVGIAGGMIRFPMWTLVHASTTHNEMATVTGLLMSVYQIGDAVGASIAG 498
Query: 343 LI 348
I
Sbjct: 499 AI 500
>sp|P46907|NARK_BACSU Nitrite extrusion protein (Nitrite facilitator)
Length = 395
Score = 38.1 bits (87), Expect = 0.020
Identities = 45/154 (29%), Positives = 69/154 (44%), Gaps = 10/154 (6%)
Frame = +1
Query: 1 GLNGADAALITSLLYIVSAACSPVMGFLIDLTG--RNLFWVLTGTLATLGSHALYAFTFA 174
GLN ADA L T+ VS P GFL D R L +V TG TL L +F+
Sbjct: 234 GLNPADAGLRTAGFIAVSTLLRPAGGFLADKMSPLRILMFVFTG--LTLSGIIL---SFS 288
Query: 175 NPLGVMIFLGVSYSVLASSLWPMVALVIP---AHQRGTAFGIMQSVQNLG-----LAVVA 330
+G+ F ++ +V + V ++P + Q G A GI+ ++ LG L + +
Sbjct: 289 PTIGLYTFGSLTVAVCSGIGNGTVFKLVPFYFSKQAGIANGIVSAMGGLGGFFPPLILAS 348
Query: 331 IIAGLIQDKYGFLILEVFFSVWLCITLVLIVLLY 432
+ Q GF+ L S + VL++ +Y
Sbjct: 349 VFQATGQYAIGFMAL----SEVALASFVLVIWMY 378
>sp|Q8Z4Z9|YFCJ_SALTI Hypothetical UPF0226 protein yfcJ
Length = 392
Score = 37.7 bits (86), Expect = 0.026
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 1/100 (1%)
Frame = +1
Query: 70 VMGFLIDLTGRNLFWVL-TGTLATLGSHALYAFTFANPLGVMIFLGVSYSVLASSLWPMV 246
V+ L++ G L W+ T +A +G+ G S++ S+L V
Sbjct: 283 VVSLLVETAGLLLLWLAPTAWIALVGA---------------ALTGAGCSLIFSALGVEV 327
Query: 247 ALVIPAHQRGTAFGIMQSVQNLGLAVVAIIAGLIQDKYGF 366
+PA RGTA G + Q++ V +AG++ YG+
Sbjct: 328 VKRVPAQVRGTALGGYAAFQDISYGVTGPLAGMLATSYGY 367
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,051,035
Number of Sequences: 369166
Number of extensions: 1484725
Number of successful extensions: 3988
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3845
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3974
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5413810770
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)