Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_F10
(776 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P00940|TPIS_CHICK Triosephosphate isomerase (TIM) (Trios... 373 e-103
sp|P54714|TPIS_CANFA Triosephosphate isomerase (TIM) (Trios... 361 1e-99
sp|Q60HC9|TPIS_MACFA Triosephosphate isomerase (TIM) (Trios... 361 1e-99
sp|P60174|TPIS_HUMAN Triosephosphate isomerase (TIM) (Trios... 360 3e-99
sp|Q589R5|TPIS_ORYLA Triosephosphate isomerase (TIM) (Trios... 360 3e-99
sp|P00939|TPIS_RABIT Triosephosphate isomerase (TIM) (Trios... 358 9e-99
sp|P48500|TPIS_RAT Triosephosphate isomerase (TIM) (Triose-... 357 2e-98
sp|P17751|TPIS_MOUSE Triosephosphate isomerase (TIM) (Trios... 353 4e-97
sp|P48501|TPIS_SCHMA Triosephosphate isomerase (TIM) (Trios... 339 4e-93
sp|P00941|TPIS_LATCH Triosephosphate isomerase (TIM) (Trios... 337 2e-92
>sp|P00940|TPIS_CHICK Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 248
Score = 373 bits (958), Expect = e-103
Identities = 182/244 (74%), Positives = 206/244 (84%)
Frame = +2
Query: 14 RKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVSA 193
RKFFVGGNWKMNGDK L ++ ++L AKL TEVV PSIYLDF RQKL AKIGV+A
Sbjct: 4 RKFFVGGNWKMNGDKKSLGELIHTLNGAKLSADTEVVCGAPSIYLDFARQKLDAKIGVAA 63
Query: 194 QNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALESGLSI 373
QNCYK+PKGAFTGEISPAMIKD WVILGHSERRHVFGESD LIG+KV HAL GL +
Sbjct: 64 QNCYKVPKGAFTGEISPAMIKDIGAAWVILGHSERRHVFGESDELIGQKVAHALAEGLGV 123
Query: 374 IPCIGEKLDERESNKTNEVCFRQLKEIAANIKDWTRVVIAYEPVWAIGTGKTASPAQAQE 553
I CIGEKLDERE+ T +V F Q K IA N+KDW++VV+AYEPVWAIGTGKTA+P QAQE
Sbjct: 124 IACIGEKLDEREAGITEKVVFEQTKAIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQE 183
Query: 554 VHKCVRDWLKTNVSEEVAKTTRILYGGSVTSGNCKELASQPDVDGFLVGGASLKPEFIDI 733
VH+ +R WLK++VS+ VA++TRI+YGGSVT GNCKELASQ DVDGFLVGGASLKPEF+DI
Sbjct: 184 VHEKLRGWLKSHVSDAVAQSTRIIYGGSVTGGNCKELASQHDVDGFLVGGASLKPEFVDI 243
Query: 734 INAK 745
INAK
Sbjct: 244 INAK 247
>sp|P54714|TPIS_CANFA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 361 bits (927), Expect = 1e-99
Identities = 175/248 (70%), Positives = 202/248 (81%)
Frame = +2
Query: 2 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 181
M+ RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL AKI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKKNLGELITTLNAAKVPADTEVVCAPPTAYIDFARQKLDAKI 60
Query: 182 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 361
V+AQNCYK+ GAFTGEISP MIKD WV+LGHSERRHVFGESD LIG+KV HAL
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAE 120
Query: 362 GLSIIPCIGEKLDERESNKTNEVCFRQLKEIAANIKDWTRVVIAYEPVWAIGTGKTASPA 541
GL +I CIGEKLDERE+ T +V F Q K IA N+KDW++VV+AYEPVWAIGTGKTA+P
Sbjct: 121 GLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQ 180
Query: 542 QAQEVHKCVRDWLKTNVSEEVAKTTRILYGGSVTSGNCKELASQPDVDGFLVGGASLKPE 721
QAQEVH+ +R WLK+NVS+ VA++TRI+YGGSVT CKELASQPDVDGFLVGGASLKPE
Sbjct: 181 QAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPE 240
Query: 722 FIDIINAK 745
F+DIINAK
Sbjct: 241 FVDIINAK 248
>sp|Q60HC9|TPIS_MACFA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
sp|P15426|TPIS_MACMU Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 361 bits (926), Expect = 1e-99
Identities = 175/248 (70%), Positives = 201/248 (81%)
Frame = +2
Query: 2 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 181
M+ RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKQNLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKI 60
Query: 182 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 361
V+AQNCYK+ GAFTGEISP MIKD WV+LGHSERRHVFGESD LIG+KV HAL
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAE 120
Query: 362 GLSIIPCIGEKLDERESNKTNEVCFRQLKEIAANIKDWTRVVIAYEPVWAIGTGKTASPA 541
GL +I CIGEKLDERE+ T +V F Q K IA N+KDW++VV+AYEPVWAIGTGKTA+P
Sbjct: 121 GLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQ 180
Query: 542 QAQEVHKCVRDWLKTNVSEEVAKTTRILYGGSVTSGNCKELASQPDVDGFLVGGASLKPE 721
QAQEVH+ +R WLK+NVSE VA++TRI+YGGSVT CKELASQPDVDGFLVGGASLKPE
Sbjct: 181 QAQEVHEKLRGWLKSNVSEAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPE 240
Query: 722 FIDIINAK 745
F+DIINAK
Sbjct: 241 FVDIINAK 248
>sp|P60174|TPIS_HUMAN Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
sp|P60175|TPIS_PANTR Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 360 bits (923), Expect = 3e-99
Identities = 174/248 (70%), Positives = 201/248 (81%)
Frame = +2
Query: 2 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 181
M+ RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKI 60
Query: 182 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 361
V+AQNCYK+ GAFTGEISP MIKD WV+LGHSERRHVFGESD LIG+KV HAL
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAE 120
Query: 362 GLSIIPCIGEKLDERESNKTNEVCFRQLKEIAANIKDWTRVVIAYEPVWAIGTGKTASPA 541
GL +I CIGEKLDERE+ T +V F Q K IA N+KDW++VV+AYEPVWAIGTGKTA+P
Sbjct: 121 GLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQ 180
Query: 542 QAQEVHKCVRDWLKTNVSEEVAKTTRILYGGSVTSGNCKELASQPDVDGFLVGGASLKPE 721
QAQEVH+ +R WLK+NVS+ VA++TRI+YGGSVT CKELASQPDVDGFLVGGASLKPE
Sbjct: 181 QAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPE 240
Query: 722 FIDIINAK 745
F+DIINAK
Sbjct: 241 FVDIINAK 248
>sp|Q589R5|TPIS_ORYLA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 248
Score = 360 bits (923), Expect = 3e-99
Identities = 172/245 (70%), Positives = 204/245 (83%)
Frame = +2
Query: 11 QRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVS 190
QR+FFVGGNWKMNG+K+ L ++ ++L A LHE TEVV A PSIYLDF R L ++I V+
Sbjct: 3 QRRFFVGGNWKMNGNKESLEELMSTLNTASLHEDTEVVCAAPSIYLDFARSGLDSRISVA 62
Query: 191 AQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALESGLS 370
AQNCYK+ KGAFTGEISPAMIKD DWVILGHSERRHVFGE+D LIG+KV HALES LS
Sbjct: 63 AQNCYKVAKGAFTGEISPAMIKDCGADWVILGHSERRHVFGENDELIGQKVAHALESDLS 122
Query: 371 IIPCIGEKLDERESNKTNEVCFRQLKEIAANIKDWTRVVIAYEPVWAIGTGKTASPAQAQ 550
+I CIGEKL+ERE+ T +V F Q + IA N+ DW +VV+AYEPVWAIGTGKTA+P QAQ
Sbjct: 123 VIACIGEKLEEREAGTTEDVIFAQTQVIAENVMDWEKVVLAYEPVWAIGTGKTATPEQAQ 182
Query: 551 EVHKCVRDWLKTNVSEEVAKTTRILYGGSVTSGNCKELASQPDVDGFLVGGASLKPEFID 730
EVH+ +R W + N+SEEV+ + RI+YGGSVT+ NC+ELASQ DVDGFLVGGASLKPEFID
Sbjct: 183 EVHEKLRAWFRANISEEVSDSLRIIYGGSVTAANCRELASQTDVDGFLVGGASLKPEFID 242
Query: 731 IINAK 745
IINA+
Sbjct: 243 IINAR 247
>sp|P00939|TPIS_RABIT Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 248
Score = 358 bits (919), Expect = 9e-99
Identities = 173/244 (70%), Positives = 199/244 (81%)
Frame = +2
Query: 14 RKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVSA 193
RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL KI V+A
Sbjct: 4 RKFFVGGNWKMNGRKKNLGELITTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAA 63
Query: 194 QNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALESGLSI 373
QNCYK+ GAFTGEISP MIKD WV+LGHSERRHVFGESD LIG+KV HAL GL +
Sbjct: 64 QNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALSEGLGV 123
Query: 374 IPCIGEKLDERESNKTNEVCFRQLKEIAANIKDWTRVVIAYEPVWAIGTGKTASPAQAQE 553
I CIGEKLDERE+ T +V F Q K IA N+KDW++VV+AYEPVWAIGTGKTA+P QAQE
Sbjct: 124 IACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQE 183
Query: 554 VHKCVRDWLKTNVSEEVAKTTRILYGGSVTSGNCKELASQPDVDGFLVGGASLKPEFIDI 733
VH+ +R WLK+NVS+ VA++TRI+YGGSVT CKELASQPDVDGFLVGGASLKPEF+DI
Sbjct: 184 VHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDI 243
Query: 734 INAK 745
INAK
Sbjct: 244 INAK 247
>sp|P48500|TPIS_RAT Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 357 bits (916), Expect = 2e-98
Identities = 175/248 (70%), Positives = 198/248 (79%)
Frame = +2
Query: 2 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 181
M+ RKFFVGGNWKMNG K L ++ +L AKL TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKKCLGELICTLNAAKLPADTEVVCAPPTAYIDFARQKLDPKI 60
Query: 182 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 361
V+AQNCYK+ GAFTGEISP MIKD WV+LGHSERRH+FGESD LIG+KV HAL
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDLGATWVVLGHSERRHIFGESDELIGQKVNHALSE 120
Query: 362 GLSIIPCIGEKLDERESNKTNEVCFRQLKEIAANIKDWTRVVIAYEPVWAIGTGKTASPA 541
GL +I CIGEKLDERE+ T +V F Q K IA N+KDW +VV+AYEPVWAIGTGKTA+P
Sbjct: 121 GLEVIACIGEKLDEREAGITEKVVFEQTKAIADNVKDWCKVVLAYEPVWAIGTGKTATPQ 180
Query: 542 QAQEVHKCVRDWLKTNVSEEVAKTTRILYGGSVTSGNCKELASQPDVDGFLVGGASLKPE 721
QAQEVH+ +R WLK NVSE VA+ TRI+YGGSVT CKELASQPDVDGFLVGGASLKPE
Sbjct: 181 QAQEVHEKLRGWLKCNVSEGVAQCTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPE 240
Query: 722 FIDIINAK 745
F+DIINAK
Sbjct: 241 FVDIINAK 248
>sp|P17751|TPIS_MOUSE Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 353 bits (905), Expect = 4e-97
Identities = 172/248 (69%), Positives = 200/248 (80%)
Frame = +2
Query: 2 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 181
M+ RKFFVGGNWKMNG K L ++ +L A + TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPTRKFFVGGNWKMNGRKKCLGELICTLNAANVPAGTEVVCAPPTAYIDFARQKLDPKI 60
Query: 182 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 361
V+AQNCYK+ GAFTGEISP MIKD WV+LGHSERRHVFGESD LIG+KV HAL
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDLGATWVVLGHSERRHVFGESDELIGQKVSHALAE 120
Query: 362 GLSIIPCIGEKLDERESNKTNEVCFRQLKEIAANIKDWTRVVIAYEPVWAIGTGKTASPA 541
GL +I CIGEKLDERE+ T +V F Q K IA N+KDW++VV+AYEPVWAIGTGKTA+P
Sbjct: 121 GLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQ 180
Query: 542 QAQEVHKCVRDWLKTNVSEEVAKTTRILYGGSVTSGNCKELASQPDVDGFLVGGASLKPE 721
QAQEVH+ +R WLK+NV++ VA++TRI+YGGSVT CKELASQPDVDGFLVGGASLKPE
Sbjct: 181 QAQEVHEKLRGWLKSNVNDGVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPE 240
Query: 722 FIDIINAK 745
F+DIINAK
Sbjct: 241 FVDIINAK 248
>sp|P48501|TPIS_SCHMA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 253
Score = 339 bits (870), Expect = 4e-93
Identities = 166/251 (66%), Positives = 193/251 (76%), Gaps = 3/251 (1%)
Frame = +2
Query: 2 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 181
MS RKFFVGGNWKMNG +D+ K+ L A + TEV++APPS++L +R+ L +I
Sbjct: 1 MSGSRKFFVGGNWKMNGSRDDNDKLLKLLSEAHFDDNTEVLIAPPSVFLHEIRKSLKKEI 60
Query: 182 GVSAQNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALES 361
V+AQNCYK+ KGAFTGEISPAMI+D CDWVILGHSERR++FGESD LI EKV+HAL
Sbjct: 61 HVAAQNCYKVSKGAFTGEISPAMIRDIGCDWVILGHSERRNIFGESDELIAEKVQHALAE 120
Query: 362 GLSIIPCIGEKLDERESNKTNEVCFRQLKEIAANIK---DWTRVVIAYEPVWAIGTGKTA 532
GLS+I CIGE L ERESNKT EVC RQLK IA IK +W RVV+AYEPVWAIGTGK A
Sbjct: 121 GLSVIACIGETLSERESNKTEEVCVRQLKAIANKIKSADEWKRVVVAYEPVWAIGTGKVA 180
Query: 533 SPAQAQEVHKCVRDWLKTNVSEEVAKTTRILYGGSVTSGNCKELASQPDVDGFLVGGASL 712
+P QAQEVH +R W KTN V + RI+YGGSVT+ NCKELA Q DVDGFLVGGASL
Sbjct: 181 TPQQAQEVHNFLRKWFKTNAPNGVDEKIRIIYGGSVTAANCKELAQQHDVDGFLVGGASL 240
Query: 713 KPEFIDIINAK 745
KPEF +I A+
Sbjct: 241 KPEFTEICKAR 251
>sp|P00941|TPIS_LATCH Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 247
Score = 337 bits (864), Expect = 2e-92
Identities = 163/239 (68%), Positives = 189/239 (79%)
Frame = +2
Query: 14 RKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVSA 193
RKFFVGGNWKMNGDK L ++ +L AK+ T E+V APP YLDF R K+ K GV+A
Sbjct: 3 RKFFVGGNWKMNGDKKSLGELIQTLNAAKVPFTGEIVCAPPEAYLDFARLKVDPKFGVAA 62
Query: 194 QNCYKIPKGAFTGEISPAMIKDNMCDWVILGHSERRHVFGESDHLIGEKVKHALESGLSI 373
QNCYK+ KGAFTGEISPAMIKD WVILGHSERRHVFGESD LIG+KV HAL GL +
Sbjct: 63 QNCYKVSKGAFTGEISPAMIKDCGVTWVILGHSERRHVFGESDELIGQKVSHALSEGLGV 122
Query: 374 IPCIGEKLDERESNKTNEVCFRQLKEIAANIKDWTRVVIAYEPVWAIGTGKTASPAQAQE 553
+ CIGEKLDERE+ T V F + IA ++KDW++VV+AYEPVWAIGTGKTASP Q+QE
Sbjct: 123 VACIGEKLDEREAGITEGVVFEVTEVIADDVKDWSKVVLAYEPVWAIGTGKTASPQQSQE 182
Query: 554 VHKCVRDWLKTNVSEEVAKTTRILYGGSVTSGNCKELASQPDVDGFLVGGASLKPEFID 730
+H +R WLK NVSE VA + RI+YGGSVT CKELAS+PDVDGFLVGGASLKPEF++
Sbjct: 183 LHGKLRKWLKENVSETVADSVRIIYGGSVTGATCKELASEPDVDGFLVGGASLKPEFVE 241
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,681,429
Number of Sequences: 369166
Number of extensions: 1442750
Number of successful extensions: 4398
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3928
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4031
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7260540000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)