Planarian EST Database


Dr_sW_016_F09-2

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_016_F09-2
         (514 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O08989|RASM_MOUSE  Ras-related protein M-Ras (Ras-related...   111   9e-25
sp|P97538|RASM_RAT  Ras-related protein M-Ras (Ras-related p...   111   9e-25
sp|O14807|RASM_HUMAN  Ras-related protein M-Ras (Ras-related...   110   1e-24
sp|P62070|RRAS2_HUMAN  Ras-related protein R-Ras2 (Ras-like ...   100   2e-21
sp|P22126|RAS1_NEUCR  Ras-1 protein                                99   8e-21
sp|P38976|RAS2_HYDMA  Ras-like protein RAS2                        98   1e-20
sp|P08647|RAS_SCHPO  Ras-like protein 1                            96   4e-20
sp|P01119|RAS1_YEAST  Ras-like protein 1                           96   4e-20
sp|P87018|RAS_BOTCI  Ras-like protein                              96   5e-20
sp|Q9UQX7|RAS1_CANAL  Ras-like protein 1 (Ras homolog type B)      96   5e-20
>sp|O08989|RASM_MOUSE Ras-related protein M-Ras (Ras-related protein R-Ras3) (Muscle and
           microspikes Ras) (X-Ras)
          Length = 208

 Score =  111 bits (278), Expect = 9e-25
 Identities = 52/101 (51%), Positives = 73/101 (72%)
 Frame = +1

Query: 211 ISVLDTAGQDSFSAMRQQYMRKGDAFLIMFSITDRNSFQMVRHLYKDILRTKDKESFPVL 390
           + VLDTAGQ+ FSAMR+QYMR GD FLI++S+TD+ SF+ V   ++ ILR KD+ESFP++
Sbjct: 63  LDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMI 122

Query: 391 IIGNKVDLXXXXXXXXXXXXXLASELNVHYMETSAKPPPIN 513
           ++ NKVDL             +A++ N+ Y+ETSAK PP+N
Sbjct: 123 LVANKVDLMHLRKVTRDQGKEMATKYNIPYIETSAKDPPLN 163

 Score = 72.8 bits (177), Expect = 5e-13
 Identities = 30/50 (60%), Positives = 40/50 (80%)
 Frame = +3

Query: 18  YVIVIIGDGAVGKTALTIQYFQRTFVEDYDPTIEDTYIQSKEFDGEWCDL 167
           Y +V++GDG VGK+ALTIQ+FQ+ FV DYDPTIED+Y++  E D +W  L
Sbjct: 14  YKLVVVGDGGVGKSALTIQFFQKIFVPDYDPTIEDSYLKHTEIDNQWAIL 63
>sp|P97538|RASM_RAT Ras-related protein M-Ras (Ras-related protein R-Ras3)
          Length = 208

 Score =  111 bits (278), Expect = 9e-25
 Identities = 52/101 (51%), Positives = 73/101 (72%)
 Frame = +1

Query: 211 ISVLDTAGQDSFSAMRQQYMRKGDAFLIMFSITDRNSFQMVRHLYKDILRTKDKESFPVL 390
           + VLDTAGQ+ FSAMR+QYMR GD FLI++S+TD+ SF+ V   ++ ILR KD+ESFP++
Sbjct: 63  LDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMI 122

Query: 391 IIGNKVDLXXXXXXXXXXXXXLASELNVHYMETSAKPPPIN 513
           ++ NKVDL             +A++ N+ Y+ETSAK PP+N
Sbjct: 123 LVANKVDLMHLRKVTRDQGKEMATKYNIPYIETSAKDPPLN 163

 Score = 72.8 bits (177), Expect = 5e-13
 Identities = 30/50 (60%), Positives = 40/50 (80%)
 Frame = +3

Query: 18  YVIVIIGDGAVGKTALTIQYFQRTFVEDYDPTIEDTYIQSKEFDGEWCDL 167
           Y +V++GDG VGK+ALTIQ+FQ+ FV DYDPTIED+Y++  E D +W  L
Sbjct: 14  YKLVVVGDGGVGKSALTIQFFQKIFVPDYDPTIEDSYLKHTEIDNQWAIL 63
>sp|O14807|RASM_HUMAN Ras-related protein M-Ras (Ras-related protein R-Ras3)
          Length = 208

 Score =  110 bits (276), Expect = 1e-24
 Identities = 52/101 (51%), Positives = 73/101 (72%)
 Frame = +1

Query: 211 ISVLDTAGQDSFSAMRQQYMRKGDAFLIMFSITDRNSFQMVRHLYKDILRTKDKESFPVL 390
           + VLDTAGQ+ FSAMR+QYMR GD FLI++S+TD+ SF+ V   ++ ILR KD+ESFP++
Sbjct: 63  LDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEHVDRFHQLILRVKDRESFPMI 122

Query: 391 IIGNKVDLXXXXXXXXXXXXXLASELNVHYMETSAKPPPIN 513
           ++ NKVDL             +A++ N+ Y+ETSAK PP+N
Sbjct: 123 LVANKVDLMHLRKITREQGKEMATKHNIPYIETSAKDPPLN 163

 Score = 72.8 bits (177), Expect = 5e-13
 Identities = 30/50 (60%), Positives = 40/50 (80%)
 Frame = +3

Query: 18  YVIVIIGDGAVGKTALTIQYFQRTFVEDYDPTIEDTYIQSKEFDGEWCDL 167
           Y +V++GDG VGK+ALTIQ+FQ+ FV DYDPTIED+Y++  E D +W  L
Sbjct: 14  YKLVVVGDGGVGKSALTIQFFQKIFVPDYDPTIEDSYLKHTEIDNQWAIL 63
>sp|P62070|RRAS2_HUMAN Ras-related protein R-Ras2 (Ras-like protein TC21) (Teratocarcinoma
           oncogene)
 sp|P62071|RRAS2_MOUSE Ras-related protein R-Ras2
          Length = 204

 Score =  100 bits (250), Expect = 2e-21
 Identities = 47/96 (48%), Positives = 64/96 (66%)
 Frame = +1

Query: 211 ISVLDTAGQDSFSAMRQQYMRKGDAFLIMFSITDRNSFQMVRHLYKDILRTKDKESFPVL 390
           + +LDTAGQ+ F AMR+QYMR G+ FL++FS+TDR SF+ +    + ILR KD++ FP++
Sbjct: 64  LDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMI 123

Query: 391 IIGNKVDLXXXXXXXXXXXXXLASELNVHYMETSAK 498
           +IGNK DL             LA +L V YME SAK
Sbjct: 124 LIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAK 159

 Score = 58.9 bits (141), Expect = 7e-09
 Identities = 26/46 (56%), Positives = 34/46 (73%)
 Frame = +3

Query: 12  QKYVIVIIGDGAVGKTALTIQYFQRTFVEDYDPTIEDTYIQSKEFD 149
           +KY +V++G G VGK+ALTIQ+ Q  FV DYDPTIED+Y +    D
Sbjct: 13  EKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVID 58
>sp|P22126|RAS1_NEUCR Ras-1 protein
          Length = 213

 Score = 98.6 bits (244), Expect = 8e-21
 Identities = 46/96 (47%), Positives = 65/96 (67%)
 Frame = +1

Query: 211 ISVLDTAGQDSFSAMRQQYMRKGDAFLIMFSITDRNSFQMVRHLYKDILRTKDKESFPVL 390
           + +LDTAGQ+ +SAMR+QYMR G+ FL++F+I  R SF+ +R   + ILR KD++SFP++
Sbjct: 58  LDILDTAGQEEYSAMREQYMRTGEGFLLVFAINSRESFEEIRIYQQQILRVKDRDSFPMI 117

Query: 391 IIGNKVDLXXXXXXXXXXXXXLASELNVHYMETSAK 498
           I+GNK DL             LA+E    Y+ETSAK
Sbjct: 118 IVGNKYDLRGERVVSEQEGQALAAEFGTKYIETSAK 153

 Score = 54.7 bits (130), Expect = 1e-07
 Identities = 27/62 (43%), Positives = 40/62 (64%)
 Frame = +3

Query: 3   KSYQKYVIVIIGDGAVGKTALTIQYFQRTFVEDYDPTIEDTYIQSKEFDGEWCDLTGLVD 182
           K  ++Y +V++G G VGK+ LTIQ  Q  F+++YDPTIED+Y +    D E      L+D
Sbjct: 4   KFTREYKLVVVGGGGVGKSCLTIQLIQGHFLDEYDPTIEDSYRKQCTIDNE----VALLD 59

Query: 183 ML 188
           +L
Sbjct: 60  IL 61
>sp|P38976|RAS2_HYDMA Ras-like protein RAS2
          Length = 192

 Score = 97.8 bits (242), Expect = 1e-20
 Identities = 46/99 (46%), Positives = 65/99 (65%)
 Frame = +1

Query: 202 VINISVLDTAGQDSFSAMRQQYMRKGDAFLIMFSITDRNSFQMVRHLYKDILRTKDKESF 381
           V ++ +LDTAGQ+ FSAMR +YMR G+ FL++FS+TDR+SF  +   +  ILR KD E F
Sbjct: 55  VAHLDILDTAGQEEFSAMRDEYMRTGEGFLLVFSVTDRSSFDEIPRFHTQILRVKDIEEF 114

Query: 382 PVLIIGNKVDLXXXXXXXXXXXXXLASELNVHYMETSAK 498
           P++++GNK DL             L  +L V Y+E+SAK
Sbjct: 115 PMILVGNKSDLENERTVSTAEAQELGRKLKVSYLESSAK 153

 Score = 58.2 bits (139), Expect = 1e-08
 Identities = 24/39 (61%), Positives = 33/39 (84%)
 Frame = +3

Query: 12  QKYVIVIIGDGAVGKTALTIQYFQRTFVEDYDPTIEDTY 128
           ++Y +V++G G VGK+ALTIQ+ Q  FV+DYDPTIED+Y
Sbjct: 7   RQYKLVVVGGGGVGKSALTIQFIQSHFVQDYDPTIEDSY 45
>sp|P08647|RAS_SCHPO Ras-like protein 1
          Length = 219

 Score = 96.3 bits (238), Expect = 4e-20
 Identities = 45/96 (46%), Positives = 65/96 (67%)
 Frame = +1

Query: 211 ISVLDTAGQDSFSAMRQQYMRKGDAFLIMFSITDRNSFQMVRHLYKDILRTKDKESFPVL 390
           + VLDTAGQ+ +SAMR+QYMR G+ FL++++IT R+SF  +   Y+ ILR KDK++FPV+
Sbjct: 58  LDVLDTAGQEEYSAMREQYMRTGEGFLLVYNITSRSSFDEISTFYQQILRVKDKDTFPVV 117

Query: 391 IIGNKVDLXXXXXXXXXXXXXLASELNVHYMETSAK 498
           ++ NK DL             LA  ++  Y+ETSAK
Sbjct: 118 LVANKCDLEAERVVSRAEGEQLAKSMHCLYVETSAK 153

 Score = 64.3 bits (155), Expect = 2e-10
 Identities = 28/48 (58%), Positives = 38/48 (79%)
 Frame = +3

Query: 12  QKYVIVIIGDGAVGKTALTIQYFQRTFVEDYDPTIEDTYIQSKEFDGE 155
           ++Y +V++GDG VGK+ALTIQ  Q  FV++YDPTIED+Y +  E DGE
Sbjct: 7   REYKLVVVGDGGVGKSALTIQLIQSHFVDEYDPTIEDSYRKKCEIDGE 54
>sp|P01119|RAS1_YEAST Ras-like protein 1
          Length = 309

 Score = 96.3 bits (238), Expect = 4e-20
 Identities = 44/96 (45%), Positives = 64/96 (66%)
 Frame = +1

Query: 211 ISVLDTAGQDSFSAMRQQYMRKGDAFLIMFSITDRNSFQMVRHLYKDILRTKDKESFPVL 390
           + +LDTAGQ+ +SAMR+QYMR G+ FL+++S+T RNSF  +   Y+ I R KD +  PV+
Sbjct: 60  LDILDTAGQEEYSAMREQYMRTGEGFLLVYSVTSRNSFDELLSYYQQIQRVKDSDYIPVV 119

Query: 391 IIGNKVDLXXXXXXXXXXXXXLASELNVHYMETSAK 498
           ++GNK+DL             LA +LN  ++ETSAK
Sbjct: 120 VVGNKLDLENERQVSYEDGLRLAKQLNAPFLETSAK 155

 Score = 58.5 bits (140), Expect = 9e-09
 Identities = 28/59 (47%), Positives = 41/59 (69%)
 Frame = +3

Query: 12  QKYVIVIIGDGAVGKTALTIQYFQRTFVEDYDPTIEDTYIQSKEFDGEWCDLTGLVDML 188
           ++Y IV++G G VGK+ALTIQ+ Q  FV++YDPTIED+Y +    D    D   ++D+L
Sbjct: 9   REYKIVVVGGGGVGKSALTIQFIQSYFVDEYDPTIEDSYRKQVVID----DKVSILDIL 63
>sp|P87018|RAS_BOTCI Ras-like protein
          Length = 212

 Score = 95.9 bits (237), Expect = 5e-20
 Identities = 46/101 (45%), Positives = 65/101 (64%)
 Frame = +1

Query: 196 ELVINISVLDTAGQDSFSAMRQQYMRKGDAFLIMFSITDRNSFQMVRHLYKDILRTKDKE 375
           E V  + VLDTAGQ+ +SAMR+QYMR G+ FL+++SIT R SF+ +    + ILR KDK+
Sbjct: 53  EEVALLDVLDTAGQEEYSAMREQYMRTGEGFLLVYSITSRQSFEEIMTFQQQILRVKDKD 112

Query: 376 SFPVLIIGNKVDLXXXXXXXXXXXXXLASELNVHYMETSAK 498
            FP++++GNK DL             LA +    ++ETSAK
Sbjct: 113 YFPIIVVGNKCDLEGERQVSKQEGQQLADDFGCKFIETSAK 153

 Score = 55.5 bits (132), Expect = 7e-08
 Identities = 28/62 (45%), Positives = 40/62 (64%)
 Frame = +3

Query: 3   KSYQKYVIVIIGDGAVGKTALTIQYFQRTFVEDYDPTIEDTYIQSKEFDGEWCDLTGLVD 182
           K  ++Y +V++G G VGK+ LTIQ  Q  FV++YDPTIED+Y +    D E      L+D
Sbjct: 4   KFLREYKLVVVGGGGVGKSCLTIQLIQSHFVDEYDPTIEDSYRKQCVIDEE----VALLD 59

Query: 183 ML 188
           +L
Sbjct: 60  VL 61
>sp|Q9UQX7|RAS1_CANAL Ras-like protein 1 (Ras homolog type B)
          Length = 290

 Score = 95.9 bits (237), Expect = 5e-20
 Identities = 46/96 (47%), Positives = 62/96 (64%)
 Frame = +1

Query: 211 ISVLDTAGQDSFSAMRQQYMRKGDAFLIMFSITDRNSFQMVRHLYKDILRTKDKESFPVL 390
           + VLDTAGQ+ +SAMR+QYMR G+ FL+++SI   NSFQ +   Y  ILR KD ++ PVL
Sbjct: 54  LDVLDTAGQEEYSAMREQYMRTGEGFLLVYSINSLNSFQELNSFYDQILRVKDSDNVPVL 113

Query: 391 IIGNKVDLXXXXXXXXXXXXXLASELNVHYMETSAK 498
           ++GNK DL             LA+  N  ++ETSAK
Sbjct: 114 VVGNKCDLEMERQVSYEDGLALANSFNCPFLETSAK 149

 Score = 55.5 bits (132), Expect = 7e-08
 Identities = 24/48 (50%), Positives = 35/48 (72%)
 Frame = +3

Query: 12  QKYVIVIIGDGAVGKTALTIQYFQRTFVEDYDPTIEDTYIQSKEFDGE 155
           ++Y +V++G G VGK+ALTIQ  Q  FV++YDPTIED+Y +    D +
Sbjct: 3   REYKLVVVGGGGVGKSALTIQLIQSHFVDEYDPTIEDSYRKQCTIDDQ 50
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,849,120
Number of Sequences: 369166
Number of extensions: 985459
Number of successful extensions: 3752
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3032
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3530
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3304927425
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)