Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_F07
(370 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O75600|KBL_HUMAN 2-amino-3-ketobutyrate coenzyme A ligas... 107 9e-24
sp|O88986|KBL_MOUSE 2-amino-3-ketobutyrate coenzyme A ligas... 106 2e-23
sp|P37419|KBL_SALTY 2-amino-3-ketobutyrate coenzyme A ligas... 94 1e-19
sp|P0AB77|KBL_ECOLI 2-amino-3-ketobutyrate coenzyme A ligas... 92 4e-19
sp|Q6GBT7|KBL_STAAS 2-amino-3-ketobutyrate coenzyme A ligas... 67 2e-11
sp|Q8EM07|KBL_OCEIH 2-amino-3-ketobutyrate coenzyme A ligas... 67 2e-11
sp|Q6GJB8|KBL_STAAR 2-amino-3-ketobutyrate coenzyme A ligas... 63 2e-10
sp|Q81V80|KBL_BACAN 2-amino-3-ketobutyrate coenzyme A ligas... 62 3e-10
sp|O31777|KBL_BACSU 2-amino-3-ketobutyrate coenzyme A ligas... 62 3e-10
sp|Q81I05|KBL_BACCR 2-amino-3-ketobutyrate coenzyme A ligas... 62 5e-10
>sp|O75600|KBL_HUMAN 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor
(AKB ligase) (Glycine acetyltransferase)
Length = 419
Score = 107 bits (267), Expect = 9e-24
Identities = 52/76 (68%), Positives = 64/76 (84%)
Frame = +2
Query: 11 GFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARIRVQISAVH 190
GF I G SHPICPV+LGDA+LAS A+ MLK+GI+VIGFSYPVVPK KARIRVQISAVH
Sbjct: 338 GFTISG-ASHPICPVMLGDARLASRMADDMLKRGIFVIGFSYPVVPKGKARIRVQISAVH 396
Query: 191 TTQQINKAIDAFRQVG 238
+ + I++ ++AF +VG
Sbjct: 397 SEEDIDRCVEAFVEVG 412
>sp|O88986|KBL_MOUSE 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor
(AKB ligase) (Glycine acetyltransferase)
Length = 416
Score = 106 bits (264), Expect = 2e-23
Identities = 50/76 (65%), Positives = 64/76 (84%)
Frame = +2
Query: 11 GFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARIRVQISAVH 190
GF + G HPICPV+LGDA+L+S+ A+ MLKKGI+VIGFSYPVVPK KARIRVQISAVH
Sbjct: 335 GFTVSG-ADHPICPVMLGDARLSSQMADDMLKKGIFVIGFSYPVVPKGKARIRVQISAVH 393
Query: 191 TTQQINKAIDAFRQVG 238
+ + I++ ++AF +VG
Sbjct: 394 SEEDIDRCVEAFVEVG 409
>sp|P37419|KBL_SALTY 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
Length = 398
Score = 93.6 bits (231), Expect = 1e-19
Identities = 46/82 (56%), Positives = 59/82 (71%)
Frame = +2
Query: 11 GFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARIRVQISAVH 190
GF + G H I PV+LGDA +A +FA + K+GIYV GF YPVVPK +ARIR Q+SA H
Sbjct: 317 GFTLAG-ADHAIIPVMLGDAVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAH 375
Query: 191 TTQQINKAIDAFRQVGTDLKVI 256
T +QI +A+DAF ++G L VI
Sbjct: 376 TPEQITRAVDAFTRIGKQLGVI 397
>sp|P0AB77|KBL_ECOLI 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
sp|P0AB79|KBL_SHIFL 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
sp|P0AB78|KBL_ECO57 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
Length = 398
Score = 92.0 bits (227), Expect = 4e-19
Identities = 45/82 (54%), Positives = 59/82 (71%)
Frame = +2
Query: 11 GFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARIRVQISAVH 190
GF + G H I PV+LGDA +A +FA + K+GIYV GF YPVVPK +ARIR Q+SA H
Sbjct: 317 GFTLAG-ADHAIIPVMLGDAVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAH 375
Query: 191 TTQQINKAIDAFRQVGTDLKVI 256
T +QI +A++AF ++G L VI
Sbjct: 376 TPEQITRAVEAFTRIGKQLGVI 397
>sp|Q6GBT7|KBL_STAAS 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
sp|Q8NXY3|KBL_STAAW 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
sp|P60121|KBL_STAAM 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
sp|P60120|KBL_STAAN 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 395
Score = 66.6 bits (161), Expect = 2e-11
Identities = 30/85 (35%), Positives = 53/85 (62%)
Frame = +2
Query: 2 TNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARIRVQIS 181
+ LG++ G+ PI PV++GD K EF++ + +G+YV +P VP+ R+R +
Sbjct: 312 SKLGYDT-GESETPITPVIIGDEKTTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPT 370
Query: 182 AVHTTQQINKAIDAFRQVGTDLKVI 256
A HT +++AI A+ +VG ++K+I
Sbjct: 371 AAHTKDMLDEAIAAYEKVGKEMKLI 395
>sp|Q8EM07|KBL_OCEIH 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 396
Score = 66.6 bits (161), Expect = 2e-11
Identities = 29/83 (34%), Positives = 53/83 (63%)
Frame = +2
Query: 8 LGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARIRVQISAV 187
LGF+I G+ PI P ++GD K A EF++ + ++G+Y +P VP+ R+R +A
Sbjct: 315 LGFDI-GESETPITPCIIGDEKKAQEFSKRLYEEGVYAKSIVFPTVPRGTGRVRNMPTAA 373
Query: 188 HTTQQINKAIDAFRQVGTDLKVI 256
HT + ++ AI ++++G ++K+I
Sbjct: 374 HTKEMLDDAIAIYKKIGQEMKLI 396
>sp|Q6GJB8|KBL_STAAR 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 395
Score = 63.2 bits (152), Expect = 2e-10
Identities = 28/85 (32%), Positives = 52/85 (61%)
Frame = +2
Query: 2 TNLGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARIRVQIS 181
+ LG++ G+ PI PV++G+ K EF++ + +G+YV +P VP+ R+R +
Sbjct: 312 SKLGYDT-GESETPITPVIIGEEKTTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPT 370
Query: 182 AVHTTQQINKAIDAFRQVGTDLKVI 256
A HT +++AI A+ +VG ++ +I
Sbjct: 371 AAHTKDMLDEAIAAYEKVGKEMNLI 395
>sp|Q81V80|KBL_BACAN 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 396
Score = 62.4 bits (150), Expect = 3e-10
Identities = 29/83 (34%), Positives = 50/83 (60%)
Frame = +2
Query: 8 LGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARIRVQISAV 187
LGFNI G+ PI P ++GD L EF++ + ++G+Y +P V K R+R +A
Sbjct: 315 LGFNI-GESETPITPCIIGDEVLTQEFSKRLNEEGVYAKSIVFPTVAKGTGRVRNMPTAA 373
Query: 188 HTTQQINKAIDAFRQVGTDLKVI 256
HT + +++AI + +VG ++ +I
Sbjct: 374 HTKEMLDEAIRKYEKVGKEMGII 396
>sp|O31777|KBL_BACSU 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 392
Score = 62.4 bits (150), Expect = 3e-10
Identities = 25/72 (34%), Positives = 47/72 (65%)
Frame = +2
Query: 41 PICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARIRVQISAVHTTQQINKAID 220
PI P+L+GD +A +F++ +L +G++ +P V K KARIR I+A HT ++++A+D
Sbjct: 321 PILPILIGDEGVAKQFSDQLLSRGVFAQSIVFPTVAKGKARIRTIITAEHTKDELDQALD 380
Query: 221 AFRQVGTDLKVI 256
+ +L+++
Sbjct: 381 VIEKTAKELQLL 392
>sp|Q81I05|KBL_BACCR 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 396
Score = 61.6 bits (148), Expect = 5e-10
Identities = 29/83 (34%), Positives = 50/83 (60%)
Frame = +2
Query: 8 LGFNIMGDMSHPICPVLLGDAKLASEFAEHMLKKGIYVIGFSYPVVPKDKARIRVQISAV 187
LGFNI G+ PI P ++GD L EF++ + ++G+Y +P V K R+R +A
Sbjct: 315 LGFNI-GESETPITPCIIGDEVLTQEFSKRLNEEGVYAKSIVFPTVAKGTGRVRNMPTAA 373
Query: 188 HTTQQINKAIDAFRQVGTDLKVI 256
HT + +++AI + +VG ++ +I
Sbjct: 374 HTKEMLDEAILKYEKVGKEMGII 396
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,456,039
Number of Sequences: 369166
Number of extensions: 665302
Number of successful extensions: 1581
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1553
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1577
length of database: 68,354,980
effective HSP length: 89
effective length of database: 51,913,565
effective search space used: 1713147645
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)