Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_E13
(451 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatas... 94 1e-19
sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatas... 91 1e-18
sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromo... 84 1e-16
sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatas... 57 1e-08
sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-V... 40 0.003
sp|Q9Y6K8|KAD5_HUMAN Adenylate kinase isoenzyme 5 (ATP-AMP ... 34 0.17
sp|P06855|KIPN_BPT4 Polynucleotide kinase (PNK) (Polynucleo... 33 0.29
sp|P41476|PNKL_NPVAC Putative bifunctional polynucleotide k... 33 0.38
sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-... 33 0.38
sp|Q920P5|KAD5_MOUSE Adenylate kinase isoenzyme 5 (ATP-AMP ... 33 0.38
>sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 521
Score = 94.4 bits (233), Expect = 1e-19
Identities = 57/143 (39%), Positives = 80/143 (55%), Gaps = 1/143 (0%)
Frame = +1
Query: 1 ADWAP-KMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCPTLM 177
A+WAP + KKDFS +DRLFALN L F TPE FFL+ F++P F P TV L
Sbjct: 295 ANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDP-RTVSRSGPLC 353
Query: 178 IKYDKFQKSASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTLKT 357
+ + SAS +++ VG+PG+GKSTF KK+L G+ +NRDTL +
Sbjct: 354 LPESRALLSASP------------EVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGS 401
Query: 358 *HKCVAETQSVFNDKEKRSGDNT 426
+CV ++ ++ + DNT
Sbjct: 402 WQRCVTTCETALKQGKRVAIDNT 424
>sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 522
Score = 90.9 bits (224), Expect = 1e-18
Identities = 58/143 (40%), Positives = 84/143 (58%), Gaps = 1/143 (0%)
Frame = +1
Query: 1 ADWAP-KMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCPTLM 177
A+WAP + KKDFS +DRLFALN L F TPE FFL+ F++P F P T+ + L
Sbjct: 294 ANWAPGRKKKDFSCADRLFALNVGLPFATPEEFFLKWPAARFELPAFDP-RTISSAGPLY 352
Query: 178 IKYDKFQKSASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTLKT 357
+ +S+S L + EVV + VG+PG+GKSTF +++L G+ +NRDTL +
Sbjct: 353 L-----PESSS-----LLSPNPEVV--VAVGFPGAGKSTFIQEHLVSAGYVHVNRDTLGS 400
Query: 358 *HKCVAETQSVFNDKEKRSGDNT 426
+CV+ Q+ ++ DNT
Sbjct: 401 WQRCVSSCQAALRQGKRVVIDNT 423
>sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromosome IV
Length = 407
Score = 84.3 bits (207), Expect = 1e-16
Identities = 56/140 (40%), Positives = 74/140 (52%), Gaps = 4/140 (2%)
Frame = +1
Query: 22 KKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCPTLMIKYDKFQK 201
KKD S +DR FA N +KF TPE FF G+ K+ E P +D
Sbjct: 186 KKDHSYADRFFAANVGVKFQTPEEFF-----GKSKVDE----------PWGPPNFDPKNL 230
Query: 202 SASDIKDILPKD----SSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTLKT*HKC 369
+ +I ++ P D SSE +ILMVG+PGSGKSTF K + ++NRDT+ T KC
Sbjct: 231 FSEEITELEPHDAQLKSSEKEIILMVGFPGSGKSTFAKMLGHQHDYKIVNRDTIGTWQKC 290
Query: 370 VAETQSVFNDKEKRSGDNTT 429
VA T+S D + DNT+
Sbjct: 291 VAATRSYLADGKSVVIDNTS 310
>sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxy-kinase ]
Length = 421
Score = 57.4 bits (137), Expect = 1e-08
Identities = 36/116 (31%), Positives = 54/116 (46%), Gaps = 1/116 (0%)
Frame = +1
Query: 28 DFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAE-TVKNCPTLMIKYDKFQKS 204
D +++D FA N +KF TPE FFL FHP V+N + + K
Sbjct: 197 DHNSTDLKFAENIGIKFETPEQFFLGHSFVPPNFESFHPKNYLVRNSSSHPYHFKK---- 252
Query: 205 ASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTLKT*HKCV 372
S ++++VG+P SGKST + + G+ +N+D LKT KC+
Sbjct: 253 -----------SEHQEIVVLVGFPSSGKSTLAESQIVTQGYERVNQDILKTKSKCI 297
>sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-VUBI intergenic region (ORF 1)
Length = 182
Score = 39.7 bits (91), Expect = 0.003
Identities = 17/25 (68%), Positives = 21/25 (84%)
Frame = +1
Query: 22 KKDFSNSDRLFALNCHLKFYTPEAF 96
+KDFS+SDR+FA N L+FYTPE F
Sbjct: 151 QKDFSDSDRVFAKNLSLQFYTPEQF 175
>sp|Q9Y6K8|KAD5_HUMAN Adenylate kinase isoenzyme 5 (ATP-AMP transphosphorylase)
Length = 198
Score = 33.9 bits (76), Expect = 0.17
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = +1
Query: 232 KDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTL 351
+D + +I ++G PGSGK T C+K +E GF ++ L
Sbjct: 6 EDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGEL 45
>sp|P06855|KIPN_BPT4 Polynucleotide kinase (PNK) (Polynucleotide 5'-hydroxy-kinase)
Length = 301
Score = 33.1 bits (74), Expect = 0.29
Identities = 16/32 (50%), Positives = 24/32 (75%), Gaps = 1/32 (3%)
Frame = +1
Query: 253 LILMVGYPGSGKSTFCKKYL-EPLGFNVINRD 345
+IL +G PGSGKST+ ++++ + GF INRD
Sbjct: 4 IILTIGCPGSGKSTWAREFIAKNPGFYNINRD 35
>sp|P41476|PNKL_NPVAC Putative bifunctional polynucleotide kinase/RNA ligase [Includes:
Polynucleotide kinase (PNK) (Polynucleotide
5'-hydroxy-kinase); RNA ligase ]
Length = 694
Score = 32.7 bits (73), Expect = 0.38
Identities = 25/82 (30%), Positives = 43/82 (52%), Gaps = 1/82 (1%)
Frame = +1
Query: 112 EGEF-KMPEFHPAETVKNCPTLMIKYDKFQKSASDIKDILPKDSSEVVLILMVGYPGSGK 288
E EF K+ E + +KN +IK+ +++ ++ K S L++++G GSGK
Sbjct: 352 ESEFLKLTEPEKIKMIKN----IIKFVSTKQALNNKLAPTIKLPSSKQLLVLIGISGSGK 407
Query: 289 STFCKKYLEPLGFNVINRDTLK 354
ST+ K G+ INRD ++
Sbjct: 408 STYAKSL---KGYTEINRDDVR 426
>sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-polynucleotidase) (DNA 3'
phosphatase) (Three prime phosphatase)
Length = 238
Score = 32.7 bits (73), Expect = 0.38
Identities = 15/26 (57%), Positives = 18/26 (69%)
Frame = +1
Query: 22 KKDFSNSDRLFALNCHLKFYTPEAFF 99
KKDFS+SD FA N H++F PE F
Sbjct: 211 KKDFSDSDIKFAENLHVEFKYPEEIF 236
>sp|Q920P5|KAD5_MOUSE Adenylate kinase isoenzyme 5 (ATP-AMP transphosphorylase)
Length = 193
Score = 32.7 bits (73), Expect = 0.38
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +1
Query: 232 KDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTL 351
+D + +I ++G PGSGK T C+K E GF ++ L
Sbjct: 2 EDLRKCKIIFLMGGPGSGKGTQCEKLAEKYGFTHLSTGEL 41
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,230,106
Number of Sequences: 369166
Number of extensions: 1088578
Number of successful extensions: 3930
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3834
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3924
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2444192520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)