Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_E12
(748 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O75600|KBL_HUMAN 2-amino-3-ketobutyrate coenzyme A ligas... 284 2e-76
sp|O88986|KBL_MOUSE 2-amino-3-ketobutyrate coenzyme A ligas... 281 2e-75
sp|P0AB77|KBL_ECOLI 2-amino-3-ketobutyrate coenzyme A ligas... 248 9e-66
sp|P37419|KBL_SALTY 2-amino-3-ketobutyrate coenzyme A ligas... 245 1e-64
sp|O31777|KBL_BACSU 2-amino-3-ketobutyrate coenzyme A ligas... 205 1e-52
sp|Q8EM07|KBL_OCEIH 2-amino-3-ketobutyrate coenzyme A ligas... 181 1e-45
sp|Q6GBT7|KBL_STAAS 2-amino-3-ketobutyrate coenzyme A ligas... 181 2e-45
sp|Q6GJB8|KBL_STAAR 2-amino-3-ketobutyrate coenzyme A ligas... 181 2e-45
sp|Q81I05|KBL_BACCR 2-amino-3-ketobutyrate coenzyme A ligas... 179 9e-45
sp|Q81V80|KBL_BACAN 2-amino-3-ketobutyrate coenzyme A ligas... 177 2e-44
>sp|O75600|KBL_HUMAN 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor
(AKB ligase) (Glycine acetyltransferase)
Length = 419
Score = 284 bits (726), Expect = 2e-76
Identities = 140/248 (56%), Positives = 187/248 (75%)
Frame = +2
Query: 5 MEALKTHGAGLSSVRFICGTTDLHIELEEKLARFHNRDAAILYASCFDANAGVFEAILSS 184
++AL+ GAGLSSVRFICGT +H LE K+ARFH R+ AILY SC+DANAG+FEA+L+
Sbjct: 89 LQALEEFGAGLSSVRFICGTQSIHKNLEAKIARFHQREDAILYPSCYDANAGLFEALLTP 148
Query: 185 EDAIISDELNHASIIDGIRLCKAKKWRYKNGDIEDLKRCVEEAKNCRLKMIVTDGVFSMD 364
EDA++SDELNHASIIDGIRLCKA K+RY++ D+ DL+ ++EA+ RL+++ TDG FSMD
Sbjct: 149 EDAVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLQEAQKHRLRLVATDGAFSMD 208
Query: 365 GKMAPLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAM 544
G +APL+EI ++ +G L+F+DECH+TGF+GPTGRG++E + V IINSTLGKA+
Sbjct: 209 GDIAPLQEICCLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQ-VTIINSTLGKAL 267
Query: 545 GGTMGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIREN 724
GG GGYT G L++ LRQ++RPYLFSN+LPP+VV + +A S +
Sbjct: 268 GGASGGYTTGPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQS-MAAK 326
Query: 725 THRFRTEM 748
T RFR++M
Sbjct: 327 TQRFRSKM 334
>sp|O88986|KBL_MOUSE 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor
(AKB ligase) (Glycine acetyltransferase)
Length = 416
Score = 281 bits (718), Expect = 2e-75
Identities = 139/248 (56%), Positives = 184/248 (74%)
Frame = +2
Query: 5 MEALKTHGAGLSSVRFICGTTDLHIELEEKLARFHNRDAAILYASCFDANAGVFEAILSS 184
++ L+ GAGLSS RFICGT +H LE K+A FH R+ AILY SCFDANAG+FEA+L+
Sbjct: 86 LQTLEEFGAGLSSTRFICGTQSIHKNLEAKIAHFHQREDAILYPSCFDANAGLFEALLTP 145
Query: 185 EDAIISDELNHASIIDGIRLCKAKKWRYKNGDIEDLKRCVEEAKNCRLKMIVTDGVFSMD 364
EDA++SDELNHASIIDGIRLCKA K+RY++ D+ DL+ ++EA+ RL+++ TDG FSMD
Sbjct: 146 EDAVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLKEAQKHRLRLVATDGAFSMD 205
Query: 365 GKMAPLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKAM 544
G +APL++I ++ +G L+FVDECH+TGF+GPTGRG++E + V IINSTLGKA+
Sbjct: 206 GDIAPLQDICRLAAQYGALVFVDECHATGFLGPTGRGTDELLGVMDQ-VTIINSTLGKAL 264
Query: 545 GGTMGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKIREN 724
GG GGYT G L++ LRQ+SRPYLFSN+LPP+VV + +A S +
Sbjct: 265 GGASGGYTTGPEPLVSLLRQRSRPYLFSNSLPPAVVGCASKALDLLMESNAIIQS-MAAK 323
Query: 725 THRFRTEM 748
T RFR++M
Sbjct: 324 TRRFRSKM 331
>sp|P0AB77|KBL_ECOLI 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
sp|P0AB79|KBL_SHIFL 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
sp|P0AB78|KBL_ECO57 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
Length = 398
Score = 248 bits (634), Expect = 9e-66
Identities = 128/251 (50%), Positives = 173/251 (68%), Gaps = 2/251 (0%)
Frame = +2
Query: 2 AMEALKTHGAGLSSVRFICGTTDLHIELEEKLARFHNRDAAILYASCFDANAGVFEAILS 181
A + +HG G++SVRFICGT D H ELE+KLA F + AILY+SCFDAN G+FE +L
Sbjct: 65 AKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGMEDAILYSSCFDANGGLFETLLG 124
Query: 182 SEDAIISDELNHASIIDGIRLCKAKKWRYKNGDIEDLKRCVEEAK--NCRLKMIVTDGVF 355
+EDAIISD LNHASIIDG+RLCKAK++RY N D+++L+ ++EA+ R +I TDGVF
Sbjct: 125 AEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQELEARLKEAREAGARHVLIATDGVF 184
Query: 356 SMDGKMAPLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLG 535
SMDG +A L+ + +++ + L+ VD+ H+ GFVG GRGS E+ D+ VDII TLG
Sbjct: 185 SMDGVIANLKGVCDLADKYDALVMVDDSHAVGFVGENGRGSHEYCDV-MGRVDIITGTLG 243
Query: 536 KAMGGTMGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKI 715
KA+GG GGYT ++ LRQ+SRPYLFSN+L P++VAASI+ ++
Sbjct: 244 KALGGASGGYTAARKEVVEWLRQRSRPYLFSNSLAPAIVAASIKV-LEMVEAGSELRDRL 302
Query: 716 RENTHRFRTEM 748
N +FR +M
Sbjct: 303 WANARQFREQM 313
>sp|P37419|KBL_SALTY 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
acetyltransferase)
Length = 398
Score = 245 bits (625), Expect = 1e-64
Identities = 127/251 (50%), Positives = 171/251 (68%), Gaps = 2/251 (0%)
Frame = +2
Query: 2 AMEALKTHGAGLSSVRFICGTTDLHIELEEKLARFHNRDAAILYASCFDANAGVFEAILS 181
A + +HG G++SVRFICGT D H LE+KLA F + AILY+SCFDAN G+FE +L
Sbjct: 65 AKAGMDSHGFGMASVRFICGTQDSHKALEQKLASFLGMEDAILYSSCFDANGGLFETLLG 124
Query: 182 SEDAIISDELNHASIIDGIRLCKAKKWRYKNGDIEDLKRCVEEAK--NCRLKMIVTDGVF 355
+EDAIISD LNHASIIDG+RLCKAK++RY N D+ +L+ ++EA+ R +I TDGVF
Sbjct: 125 AEDAIISDALNHASIIDGVRLCKAKRYRYANNDMAELEARLKEAREAGARHVLIATDGVF 184
Query: 356 SMDGKMAPLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLG 535
SMDG +A L+ + +++ + L+ VD+ H+ GFVG GRGS E+ D+ VDII TLG
Sbjct: 185 SMDGVIANLKGVCDLADKYDALVMVDDSHAVGFVGENGRGSHEYCDV-MGRVDIITGTLG 243
Query: 536 KAMGGTMGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQAXXXXXXXXXXXXSKI 715
KA+GG GGYT ++ LRQ+SRPYLFSN+L P++VAASI+ ++
Sbjct: 244 KALGGASGGYTAARKEVVEWLRQRSRPYLFSNSLAPAIVAASIKV-LEMVEAGAELRDRL 302
Query: 716 RENTHRFRTEM 748
N +FR +M
Sbjct: 303 WANARQFREQM 313
>sp|O31777|KBL_BACSU 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 392
Score = 205 bits (521), Expect = 1e-52
Identities = 110/223 (49%), Positives = 153/223 (68%)
Frame = +2
Query: 2 AMEALKTHGAGLSSVRFICGTTDLHIELEEKLARFHNRDAAILYASCFDANAGVFEAILS 181
A EA++ +GAG SVR I GT +H ELE+KLA F +AA+++ S F N GV +ILS
Sbjct: 63 AQEAVQQYGAGTGSVRTIAGTFTMHQELEKKLAAFKKTEAALVFQSGFTTNQGVLSSILS 122
Query: 182 SEDAIISDELNHASIIDGIRLCKAKKWRYKNGDIEDLKRCVEEAKNCRLKMIVTDGVFSM 361
ED +ISDELNHASIIDGIRL KA K Y++ ++ DL+R + ++ N R+++IVTDGVFSM
Sbjct: 123 KEDIVISDELNHASIIDGIRLTKADKKVYQHVNMSDLERVLRKSMNYRMRLIVTDGVFSM 182
Query: 362 DGKMAPLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTLGKA 541
DG +APL +I+ ++E + + VD+ H++G +G GRG+ + L V I TL KA
Sbjct: 183 DGNIAPLPDIVELAEKYDAFVMVDDAHASGVLGENGRGTVNHFGL-DGRVHIQVGTLSKA 241
Query: 542 MGGTMGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQA 670
+ G +GGY GS LI+ LR K RP+LFS + PP+V AA ++A
Sbjct: 242 I-GVLGGYAAGSKVLIDYLRHKGRPFLFSTSHPPAVTAACMEA 283
>sp|Q8EM07|KBL_OCEIH 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 396
Score = 181 bits (460), Expect = 1e-45
Identities = 102/226 (45%), Positives = 145/226 (64%), Gaps = 3/226 (1%)
Frame = +2
Query: 2 AMEALKTHGAGLSSVRFICGTTDLHIELEEKLARFHNRDAAILYASCFDANAGVFEAILS 181
A +A+ +HG G +VR I GT DLHIELE+K+A+F +AAI Y S F+ N A++
Sbjct: 65 AKKAIDSHGVGAGAVRTINGTLDLHIELEKKIAQFKGTEAAIAYQSGFNCNMAAISAVMD 124
Query: 182 SEDAIISDELNHASIIDGIRLCKAKKWRYKNGDIEDLKRCVEEAKNC---RLKMIVTDGV 352
DAI+SD LNHASIIDG RL KAK R+++ D+ DL++ +EA M++TDGV
Sbjct: 125 KNDAILSDSLNHASIIDGCRLSKAKIIRFEHSDMNDLRQKAKEAVESGQYNKIMVITDGV 184
Query: 353 FSMDGKMAPLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTL 532
FSMDG +A L EI+ ++E+ + +VD+ H +G G G G+ + + L Q VD+ TL
Sbjct: 185 FSMDGDIAKLPEIVDIAEEFDLITYVDDAHGSGVTG-DGAGTVKHFGL-QDKVDMQMGTL 242
Query: 533 GKAMGGTMGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQA 670
KA+ G +GGY G SLI+ L+ +SRP+LFS + P+ AS +A
Sbjct: 243 SKAI-GVIGGYVAGKQSLIDWLKVRSRPFLFSTAVSPADAVASKRA 287
>sp|Q6GBT7|KBL_STAAS 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
sp|Q8NXY3|KBL_STAAW 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
sp|P60121|KBL_STAAM 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
sp|P60120|KBL_STAAN 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 395
Score = 181 bits (458), Expect = 2e-45
Identities = 104/226 (46%), Positives = 141/226 (62%), Gaps = 3/226 (1%)
Frame = +2
Query: 2 AMEALKTHGAGLSSVRFICGTTDLHIELEEKLARFHNRDAAILYASCFDANAGVFEAILS 181
A A+ THG G +VR I GT DLH ELEE LA+F +AAI Y S F+ N A+++
Sbjct: 64 AKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTEAAIAYQSGFNCNMAAISAVMN 123
Query: 182 SEDAIISDELNHASIIDGIRLCKAKKWRYKNGDIEDLKRCVEEAKNC---RLKMIVTDGV 352
DAI+SDELNHASIIDG RL KAK R + D++DL+ +EA M +TDGV
Sbjct: 124 KNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDLRAKAKEAVESGQYNKVMYITDGV 183
Query: 353 FSMDGKMAPLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTL 532
FSMDG +A L EI+ ++E+ G L +VD+ H +G +G G G+ + + L Q +D TL
Sbjct: 184 FSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVMG-KGAGTVKHFGL-QDKIDFQIGTL 241
Query: 533 GKAMGGTMGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQA 670
KA+ G +GGY G+ LI+ L+ +SRP+LFS +L P A +A
Sbjct: 242 SKAI-GVVGGYVAGTKELIDWLKAQSRPFLFSTSLAPGDTKAITEA 286
>sp|Q6GJB8|KBL_STAAR 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 395
Score = 181 bits (458), Expect = 2e-45
Identities = 104/226 (46%), Positives = 141/226 (62%), Gaps = 3/226 (1%)
Frame = +2
Query: 2 AMEALKTHGAGLSSVRFICGTTDLHIELEEKLARFHNRDAAILYASCFDANAGVFEAILS 181
A A+ THG G +VR I GT DLH ELEE LA+F +AAI Y S F+ N A+++
Sbjct: 64 AKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTEAAIAYQSGFNCNMAAISAVMN 123
Query: 182 SEDAIISDELNHASIIDGIRLCKAKKWRYKNGDIEDLKRCVEEAKNC---RLKMIVTDGV 352
DAI+SDELNHASIIDG RL KAK R + D++DL+ +EA M +TDGV
Sbjct: 124 KNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDLRAKAKEAVESGQYNKVMYITDGV 183
Query: 353 FSMDGKMAPLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTL 532
FSMDG +A L EI+ ++E+ G L +VD+ H +G +G G G+ + + L Q +D TL
Sbjct: 184 FSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVMG-KGAGTVKHFGL-QDKIDFQIGTL 241
Query: 533 GKAMGGTMGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQA 670
KA+ G +GGY G+ LI+ L+ +SRP+LFS +L P A +A
Sbjct: 242 SKAI-GVVGGYVAGTKELIDWLKAQSRPFLFSTSLAPGDTKAITEA 286
>sp|Q81I05|KBL_BACCR 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 396
Score = 179 bits (453), Expect = 9e-45
Identities = 102/226 (45%), Positives = 145/226 (64%), Gaps = 3/226 (1%)
Frame = +2
Query: 2 AMEALKTHGAGLSSVRFICGTTDLHIELEEKLARFHNRDAAILYASCFDANAGVFEAILS 181
A+ A+ +G G +VR I GT DLHI+LEE +A+F + +AAI Y S F+ N A++
Sbjct: 65 AIGAIHKYGVGAGAVRTINGTLDLHIKLEETIAKFKHTEAAIAYQSGFNCNMAAISAVMD 124
Query: 182 SEDAIISDELNHASIIDGIRLCKAKKWRYKNGDIEDLKRCVEEAKNCRL---KMIVTDGV 352
DAI+SDELNHASIIDG RL KAK YK+ D+EDL++ AK L M++TDGV
Sbjct: 125 KNDAILSDELNHASIIDGSRLSKAKIIVYKHSDMEDLRQKAIAAKESGLYNKLMVITDGV 184
Query: 353 FSMDGKMAPLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTL 532
FSMDG +A L EI+ ++E+ + +VD+ H +G +G G G+ + + L VD TL
Sbjct: 185 FSMDGDVAKLPEIVEIAEELDLMTYVDDAHGSGVLG-KGAGTVKHFGLSDK-VDFQIGTL 242
Query: 533 GKAMGGTMGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQA 670
KA+ G +GGY G +LI+ L+ +SRP+LFS L P+ AA +++
Sbjct: 243 SKAI-GVIGGYVAGKQNLIDWLKVRSRPFLFSTALTPADAAACMRS 287
>sp|Q81V80|KBL_BACAN 2-amino-3-ketobutyrate coenzyme A ligase (AKB ligase) (Glycine
C-acetyltransferase)
Length = 396
Score = 177 bits (450), Expect = 2e-44
Identities = 101/226 (44%), Positives = 145/226 (64%), Gaps = 3/226 (1%)
Frame = +2
Query: 2 AMEALKTHGAGLSSVRFICGTTDLHIELEEKLARFHNRDAAILYASCFDANAGVFEAILS 181
A+ A+ +G G +VR I GT DLHI+LEE +A+F + +AAI Y S F+ N A++
Sbjct: 65 AIGAIHKYGVGAGAVRTINGTLDLHIKLEETIAKFKHTEAAIAYQSGFNCNMAAISAVMD 124
Query: 182 SEDAIISDELNHASIIDGIRLCKAKKWRYKNGDIEDLKRCVEEAKNCRL---KMIVTDGV 352
DAI+SDELNHASIIDG RL KAK YK+ D+EDL++ AK L M++TDGV
Sbjct: 125 KNDAILSDELNHASIIDGSRLSKAKIIVYKHSDMEDLRQKAIAAKESGLYNKLMVITDGV 184
Query: 353 FSMDGKMAPLREILAVSEDHGCLMFVDECHSTGFVGPTGRGSEEFWDLHQPCVDIINSTL 532
FSMDG +A L EI+ ++E+ + +VD+ H +G +G G G+ + + L VD TL
Sbjct: 185 FSMDGDVAKLPEIVEIAEELDLMTYVDDAHGSGVLG-KGAGTVKHFGLSDK-VDFQIGTL 242
Query: 533 GKAMGGTMGGYTVGSNSLINTLRQKSRPYLFSNTLPPSVVAASIQA 670
KA+ G +GGY G +LI+ L+ +SRP+LFS + P+ AA +++
Sbjct: 243 SKAI-GVIGGYVAGKQNLIDWLKVRSRPFLFSTAVTPADAAACMRS 287
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,404,764
Number of Sequences: 369166
Number of extensions: 1874189
Number of successful extensions: 5098
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4842
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4966
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6776504000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)