Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_C13
(883 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 174 4e-43
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 174 4e-43
sp|P27636|CDC15_YEAST Cell division control protein 15 171 2e-42
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 169 7e-42
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 166 6e-41
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 163 7e-40
sp|P41892|CDC7_SCHPO Cell division control protein 7 160 4e-39
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 160 6e-39
sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (... 157 3e-38
sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (... 157 4e-38
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 174 bits (440), Expect = 4e-43
Identities = 103/260 (39%), Positives = 155/260 (59%), Gaps = 8/260 (3%)
Frame = -1
Query: 868 IGRGAFGTVFKTKESQSDREFAVKTLKIMLND---NFNIQALSLERDLNKYREKIQHERI 698
+G+GAFG V+ + + RE A K ++ + + + AL E L K +QHERI
Sbjct: 368 LGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLK---NLQHERI 424
Query: 697 VKYFGAIR--TQKKIYIFMEYMPGGSIRRQIIKNENKPLPEIQVKKYTKQILEGLQFLHQ 524
V+Y+G +R +K + IFMEYMPGGS++ Q+ L E +KYT+QILEG+ +LH
Sbjct: 425 VQYYGCLRDRAEKILTIFMEYMPGGSVKDQL--KAYGALTESVTRKYTRQILEGMSYLHS 482
Query: 523 SDIIHRDIKSENILLDSNGNAKLADFGLSSFVKTVVKD--KVRNLCGTANYIAPEIINDE 350
+ I+HRDIK NIL DS GN KL DFG S ++T+ +R++ GT +++PE+I+ E
Sbjct: 483 NMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPYWMSPEVISGE 542
Query: 349 SYDFKVDIWSVGATIIEMLTGFPPYHGIHESAVIFKVG-QNRKIEYPSELTNDFKNLLDQ 173
Y K D+WS+G T++EMLT PP+ A IFK+ Q + PS ++ ++ L +
Sbjct: 543 GYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFL-R 601
Query: 172 MLTTDPTKRSSADELLKSAF 113
+ + +R SA+ELL F
Sbjct: 602 RIFVEARQRPSAEELLTHHF 621
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 174 bits (440), Expect = 4e-43
Identities = 103/260 (39%), Positives = 155/260 (59%), Gaps = 8/260 (3%)
Frame = -1
Query: 868 IGRGAFGTVFKTKESQSDREFAVKTLKIMLND---NFNIQALSLERDLNKYREKIQHERI 698
+G+GAFG V+ + + RE A K ++ + + + AL E L K +QHERI
Sbjct: 368 LGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLK---NLQHERI 424
Query: 697 VKYFGAIR--TQKKIYIFMEYMPGGSIRRQIIKNENKPLPEIQVKKYTKQILEGLQFLHQ 524
V+Y+G +R +K + IFMEYMPGGS++ Q+ L E +KYT+QILEG+ +LH
Sbjct: 425 VQYYGCLRDRAEKTLTIFMEYMPGGSVKDQL--KAYGALTESVTRKYTRQILEGMSYLHS 482
Query: 523 SDIIHRDIKSENILLDSNGNAKLADFGLSSFVKTVVKD--KVRNLCGTANYIAPEIINDE 350
+ I+HRDIK NIL DS GN KL DFG S ++T+ +R++ GT +++PE+I+ E
Sbjct: 483 NMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGE 542
Query: 349 SYDFKVDIWSVGATIIEMLTGFPPYHGIHESAVIFKVG-QNRKIEYPSELTNDFKNLLDQ 173
Y K D+WS+G T++EMLT PP+ A IFK+ Q + PS ++ ++ L +
Sbjct: 543 GYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFL-R 601
Query: 172 MLTTDPTKRSSADELLKSAF 113
+ + +R SA+ELL F
Sbjct: 602 RIFVEARQRPSAEELLTHHF 621
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 171 bits (434), Expect = 2e-42
Identities = 98/256 (38%), Positives = 146/256 (57%), Gaps = 2/256 (0%)
Frame = -1
Query: 868 IGRGAFGTVFKTKESQSDREFAVKTLKIMLNDNFN--IQALSLERDLNKYREKIQHERIV 695
IGRG++G V+K +D+ A+K + ++ N + +SL ++LN H IV
Sbjct: 31 IGRGSYGVVYKAINKHTDQVVAIKEVVYENDEELNDIMAEISLLKNLN-------HNNIV 83
Query: 694 KYFGAIRTQKKIYIFMEYMPGGSIRRQIIKNENKPLPEIQVKKYTKQILEGLQFLHQSDI 515
KY G IR ++YI +EY GS+RR +I + L E + K Y Q L GL++LH +
Sbjct: 84 KYHGFIRKSYELYILLEYCANGSLRR-LISRSSTGLSENESKTYVTQTLLGLKYLHGEGV 142
Query: 514 IHRDIKSENILLDSNGNAKLADFGLSSFVKTVVKDKVRNLCGTANYIAPEIINDESYDFK 335
IHRDIK+ NILL ++ KLADFG+S T+V L GT N++APEI+ +
Sbjct: 143 IHRDIKAANILLSADNTVKLADFGVS----TIVNSSALTLAGTLNWMAPEILGNRGASTL 198
Query: 334 VDIWSVGATIIEMLTGFPPYHGIHESAVIFKVGQNRKIEYPSELTNDFKNLLDQMLTTDP 155
DIWS+GAT++EMLT PPYH + ++ + + V +N PS + K+ L + +
Sbjct: 199 SDIWSLGATVVEMLTKNPPYHNLTDANIYYAV-ENDTYYPPSSFSEPLKDFLSKCFVKNM 257
Query: 154 TKRSSADELLKSAFFN 107
KR +AD+LLK + N
Sbjct: 258 YKRPTADQLLKHVWIN 273
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 169 bits (429), Expect = 7e-42
Identities = 105/266 (39%), Positives = 155/266 (58%), Gaps = 14/266 (5%)
Frame = -1
Query: 868 IGRGAFGTVFKTKESQSDREFAVKTLKIMLNDNFNIQALSLERDLNKYREKIQ------H 707
+G+GAFG V+ + + RE AVK ++ F+ + +++N +IQ H
Sbjct: 361 LGQGAFGRVYLCYDVDTGRELAVKQVQ------FDPDSPETSKEVNALECEIQLLKNFLH 414
Query: 706 ERIVKYFGAIRT--QKKIYIFMEYMPGGSIRRQIIKNENKPLPEIQVKKYTKQILEGLQF 533
ERIV+Y+G +R +K + IFMEYMPGGSI+ Q+ L E +KYT+QILEG+ +
Sbjct: 415 ERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQL--KAYGALTENGTRKYTRQILEGVHY 472
Query: 532 LHQSDIIHRDIKSENILLDSNGNAKLADFGLSSFVKTVVKD--KVRNLCGTANYIAPEII 359
LH + I+HRDIK NIL DS GN KL DFG S ++T+ ++++ GT +++PE+I
Sbjct: 473 LHSNMILHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVI 532
Query: 358 NDESYDFKVDIWSVGATIIEMLTGFPPYHGIHESAVIFKVG---QNRKI-EYPSELTNDF 191
+ + Y K DIWSV T++EMLT PP+ A IFK+ N K+ + S+ T DF
Sbjct: 533 SGQGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDF 592
Query: 190 KNLLDQMLTTDPTKRSSADELLKSAF 113
+ + + R SADELL+ F
Sbjct: 593 L----KRIFVEAKLRPSADELLRHMF 614
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 166 bits (421), Expect = 6e-41
Identities = 104/266 (39%), Positives = 154/266 (57%), Gaps = 14/266 (5%)
Frame = -1
Query: 868 IGRGAFGTVFKTKESQSDREFAVKTLKIMLNDNFNIQALSLERDLNKYREKIQ------H 707
+G+GAFG V+ + + RE AVK ++ FN ++ +++N +IQ H
Sbjct: 362 LGQGAFGRVYLCYDVDTGRELAVKQVQ------FNPESPETSKEVNALECEIQLLKNLLH 415
Query: 706 ERIVKYFGAIRT--QKKIYIFMEYMPGGSIRRQIIKNENKPLPEIQVKKYTKQILEGLQF 533
ERIV+Y+G +R +K + IFME PGGSI+ Q+ L E +KYT+QILEG+ +
Sbjct: 416 ERIVQYYGCLRDPQEKTLSIFMELSPGGSIKDQL--KAYGALTENVTRKYTRQILEGVHY 473
Query: 532 LHQSDIIHRDIKSENILLDSNGNAKLADFGLSSFVKTVVKD--KVRNLCGTANYIAPEII 359
LH + I+HRDIK NIL DS GN KL DFG S ++T+ ++++ GT +++PE+I
Sbjct: 474 LHSNMIVHRDIKGANILRDSTGNIKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVI 533
Query: 358 NDESYDFKVDIWSVGATIIEMLTGFPPYHGIHESAVIFKVG---QNRKI-EYPSELTNDF 191
+ E Y K DIWSV T++EMLT PP+ A IFK+ N K+ + S+ T DF
Sbjct: 534 SGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDF 593
Query: 190 KNLLDQMLTTDPTKRSSADELLKSAF 113
+ + + R SA+ELL+ F
Sbjct: 594 L----KRIFVEAKLRPSAEELLRHMF 615
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 163 bits (412), Expect = 7e-40
Identities = 103/263 (39%), Positives = 143/263 (54%), Gaps = 7/263 (2%)
Frame = -1
Query: 880 KTDCIGRGAFGTVFKTKESQSDREFAVKTLKIMLN----DNFNIQALSLERDLNKYREKI 713
K IGRGAFGTV+ S AVK + I N + LE ++ K + +
Sbjct: 71 KGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEV-KLLKNL 129
Query: 712 QHERIVKYFGAIRTQKKIYIFMEYMPGGSIRRQIIKNENKPLPEIQVKKYTKQILEGLQF 533
H IV+Y G +R + I +E++PGGSI + K P PE V+ YT+Q+L GL++
Sbjct: 130 SHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFG--PFPESVVRTYTRQLLLGLEY 187
Query: 532 LHQSDIIHRDIKSENILLDSNGNAKLADFGLSSFV-KTVVKDKVRNLCGTANYIAPEIIN 356
LH I+HRDIK NIL+D+ G KLADFG S V + +++ GT ++APE+I
Sbjct: 188 LHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWMAPEVIL 247
Query: 355 DESYDFKVDIWSVGATIIEMLTGFPPY-HGIHESAVIFKVGQNRK-IEYPSELTNDFKNL 182
+ F DIWSVG T+IEM+TG P+ E A IF +G + P L++D K+
Sbjct: 248 QTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDAKDF 307
Query: 181 LDQMLTTDPTKRSSADELLKSAF 113
L + L P R +A ELLK F
Sbjct: 308 LLKCLQEVPNLRPTASELLKHPF 330
>sp|P41892|CDC7_SCHPO Cell division control protein 7
Length = 1062
Score = 160 bits (405), Expect = 4e-39
Identities = 93/254 (36%), Positives = 142/254 (55%)
Frame = -1
Query: 883 TKTDCIGRGAFGTVFKTKESQSDREFAVKTLKIMLNDNFNIQALSLERDLNKYREKIQHE 704
T DC+G+GAFG V++ ++ AVK +K+ ++ + +E DL K + H
Sbjct: 10 TLGDCLGKGAFGAVYRGLNIKNGETVAVKKVKLSKMLKSDLSVIKMEIDLLK---NLDHP 66
Query: 703 RIVKYFGAIRTQKKIYIFMEYMPGGSIRRQIIKNENKPLPEIQVKKYTKQILEGLQFLHQ 524
IVKY G+ +T + I +EY GS+R I KN K +PE V YT Q+L+GL +LH
Sbjct: 67 NIVKYRGSYQTNDSLCIILEYCENGSLR-SICKNFGK-IPENLVALYTFQVLQGLLYLHN 124
Query: 523 SDIIHRDIKSENILLDSNGNAKLADFGLSSFVKTVVKDKVRNLCGTANYIAPEIINDESY 344
+IHRDIK NIL +G KLADFG+++ + + V G+ ++APE+I
Sbjct: 125 QGVIHRDIKGANILTTKDGTIKLADFGVATKINALEDHSV---VGSPYWMAPEVIELVGA 181
Query: 343 DFKVDIWSVGATIIEMLTGFPPYHGIHESAVIFKVGQNRKIEYPSELTNDFKNLLDQMLT 164
DIWSVG T+IE+L G PPY+ + ++ +F++ ++ PS +++ K+ L Q
Sbjct: 182 TTASDIWSVGCTVIELLDGNPPYYDLDPTSALFRMVKDEHPPLPSNISSAAKSFLMQCFQ 241
Query: 163 TDPTKRSSADELLK 122
DP R +LLK
Sbjct: 242 KDPNLRIKTRKLLK 255
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 160 bits (404), Expect = 6e-39
Identities = 100/263 (38%), Positives = 142/263 (53%), Gaps = 7/263 (2%)
Frame = -1
Query: 880 KTDCIGRGAFGTVFKTKESQSDREFAVKTLKIMLN----DNFNIQALSLERDLNKYREKI 713
K IGRGAFGTV+ S AVK + I N + LE ++ K + +
Sbjct: 70 KGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEV-KLLKNL 128
Query: 712 QHERIVKYFGAIRTQKKIYIFMEYMPGGSIRRQIIKNENKPLPEIQVKKYTKQILEGLQF 533
H IV+Y G +R + + I +E++PGGSI + K PE V+ YT Q+L GL++
Sbjct: 129 SHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFG--AFPESVVRTYTNQLLLGLEY 186
Query: 532 LHQSDIIHRDIKSENILLDSNGNAKLADFGLSSFVKTVVK-DKVRNLCGTANYIAPEIIN 356
LH I+HRDIK NIL+D+ G KLADFG S V + +++ GT ++APE+I
Sbjct: 187 LHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAPEVIL 246
Query: 355 DESYDFKVDIWSVGATIIEMLTGFPPY-HGIHESAVIFKVGQNRK-IEYPSELTNDFKNL 182
+ F DIWSVG T+IEM+TG P+ E A IF +G + P +++D +
Sbjct: 247 QTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNISSDANDF 306
Query: 181 LDQMLTTDPTKRSSADELLKSAF 113
L + L +P R +A ELLK F
Sbjct: 307 LLKCLQQEPNLRPTASELLKHPF 329
>sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
(Serine/threonine-protein kinase MASK) (Mst3 and
SOK1-related kinase)
Length = 416
Score = 157 bits (398), Expect = 3e-38
Identities = 89/257 (34%), Positives = 140/257 (54%)
Frame = -1
Query: 883 TKTDCIGRGAFGTVFKTKESQSDREFAVKTLKIMLNDNFNIQALSLERDLNKYREKIQHE 704
TK + IG+G+FG VFK ++++ + A+K + + + + +++++ +
Sbjct: 25 TKLERIGKGSFGEVFKGIDNRTQQVVAIKIIDL---EEAEDEIEDIQQEITVLSQ-CDSS 80
Query: 703 RIVKYFGAIRTQKKIYIFMEYMPGGSIRRQIIKNENKPLPEIQVKKYTKQILEGLQFLHQ 524
+ KY+G+ K++I MEY+ GGS + P E Q+ K+IL+GL +LH
Sbjct: 81 YVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRAG---PFDEFQIATMLKEILKGLDYLHS 137
Query: 523 SDIIHRDIKSENILLDSNGNAKLADFGLSSFVKTVVKDKVRNLCGTANYIAPEIINDESY 344
IHRDIK+ N+LL G+ KLADFG++ + T + K GT ++APE+I +Y
Sbjct: 138 EKKIHRDIKAANVLLSEQGDVKLADFGVAGQL-TDTQIKRNTFVGTPFWMAPEVIQQSAY 196
Query: 343 DFKVDIWSVGATIIEMLTGFPPYHGIHESAVIFKVGQNRKIEYPSELTNDFKNLLDQMLT 164
D K DIWS+G T IE+ G PP +H V+F + +N + T FK +D L
Sbjct: 197 DSKADIWSLGITAIELAKGEPPNSDMHPMRVLFLIPKNNPPTLVGDFTKSFKEFIDACLN 256
Query: 163 TDPTKRSSADELLKSAF 113
DP+ R +A ELLK F
Sbjct: 257 KDPSFRPTAKELLKHKF 273
>sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
Length = 416
Score = 157 bits (397), Expect = 4e-38
Identities = 89/257 (34%), Positives = 140/257 (54%)
Frame = -1
Query: 883 TKTDCIGRGAFGTVFKTKESQSDREFAVKTLKIMLNDNFNIQALSLERDLNKYREKIQHE 704
TK + IG+G+FG VFK ++++ + A+K + + + + +++++ +
Sbjct: 25 TKLERIGKGSFGEVFKGIDNRTQQVVAIKIIDL---EEAEDEIEDIQQEITVLSQ-CDSS 80
Query: 703 RIVKYFGAIRTQKKIYIFMEYMPGGSIRRQIIKNENKPLPEIQVKKYTKQILEGLQFLHQ 524
+ KY+G+ K++I MEY+ GGS + P E Q+ K+IL+GL +LH
Sbjct: 81 YVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRAG---PFDEFQIATMLKEILKGLDYLHS 137
Query: 523 SDIIHRDIKSENILLDSNGNAKLADFGLSSFVKTVVKDKVRNLCGTANYIAPEIINDESY 344
IHRDIK+ N+LL G+ KLADFG++ + T + K GT ++APE+I +Y
Sbjct: 138 EKKIHRDIKAANVLLSEQGDVKLADFGVAGQL-TDTQIKRNTFVGTPFWMAPEVIQQSAY 196
Query: 343 DFKVDIWSVGATIIEMLTGFPPYHGIHESAVIFKVGQNRKIEYPSELTNDFKNLLDQMLT 164
D K DIWS+G T IE+ G PP +H V+F + +N + T FK +D L
Sbjct: 197 DSKADIWSLGITAIELAKGEPPNSDMHPMRVLFLIPKNNPPTLIGDFTKSFKEFIDACLN 256
Query: 163 TDPTKRSSADELLKSAF 113
DP+ R +A ELLK F
Sbjct: 257 KDPSFRPTAKELLKHKF 273
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,462,528
Number of Sequences: 369166
Number of extensions: 1930226
Number of successful extensions: 10334
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6622
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7031
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8790245790
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)