Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_B18
(877 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P41035|IF2B_RABIT Eukaryotic translation initiation fact... 42 0.003
sp|P20042|IF2B_HUMAN Eukaryotic translation initiation fact... 40 0.007
sp|P23615|SPT6_YEAST Transcription initiation protein SPT6 39 0.015
sp|P53843|YN05_YEAST Hypothetical 34.5 kDa protein in PIK1-... 39 0.025
sp|Q99L45|IF2B_MOUSE Eukaryotic translation initiation fact... 38 0.032
sp|Q38433|ALT_BPT6 NAD--protein ADP-ribosyltransferase (Alt... 38 0.032
sp|P39922|MYS3_HYDAT Myosin heavy chain, clone 203 37 0.094
sp|Q38424|ALT_BPT2 NAD--protein ADP-ribosyltransferase (Alt... 36 0.12
sp|P08116|PVA_PLAFA Processed variable antigen 35 0.21
sp|Q02455|MLP1_YEAST Protein MLP1 (Myosin-like protein 1) 35 0.27
>sp|P41035|IF2B_RABIT Eukaryotic translation initiation factor 2 subunit 2 (Eukaryotic
translation initiation factor 2 beta subunit)
(eIF-2-beta)
Length = 333
Score = 41.6 bits (96), Expect = 0.003
Identities = 27/85 (31%), Positives = 44/85 (51%), Gaps = 2/85 (2%)
Frame = +2
Query: 275 DPIFSDKTKLFKQEKDKKPFLI-LEGDGIDEEQADVKYIVTETNATEDWNLKIENQSEKT 451
D + D T K++K KKPF++ EGD EE ++ E TED +++ + + +
Sbjct: 4 DEMIFDPTMSKKKKKKKKPFMLDEEGDAQTEETQPLETKEVEPEPTEDKDVEADEEDSRK 63
Query: 452 GSNSDDNHELT-VNQKSKLKNYEQL 523
SDD +L NQK K K +++
Sbjct: 64 KDASDDLDDLNFFNQKKKKKKTKKI 88
>sp|P20042|IF2B_HUMAN Eukaryotic translation initiation factor 2 subunit 2 (Eukaryotic
translation initiation factor 2 beta subunit)
(eIF-2-beta)
Length = 333
Score = 40.4 bits (93), Expect = 0.007
Identities = 28/85 (32%), Positives = 43/85 (50%), Gaps = 2/85 (2%)
Frame = +2
Query: 275 DPIFSDKTKLFKQEKDKKPFLI-LEGDGIDEEQADVKYIVTETNATEDWNLKIENQSEKT 451
D + D T K++K KKPF++ EGD EE + E TED +L+ + + +
Sbjct: 4 DEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRK 63
Query: 452 GSNSDDNHELT-VNQKSKLKNYEQL 523
SDD +L NQK K K +++
Sbjct: 64 KDASDDLDDLNFFNQKKKKKKTKKI 88
>sp|P23615|SPT6_YEAST Transcription initiation protein SPT6
Length = 1451
Score = 39.3 bits (90), Expect = 0.015
Identities = 30/123 (24%), Positives = 63/123 (51%), Gaps = 3/123 (2%)
Frame = +2
Query: 140 NEYEDEDKLHNSIYNSNKTEDIKQVVVNEVKDTFS---FSIKSESCKVDPIFSDKTKLFK 310
NEY +++ H N N+T D K +++E+ D FS + + + I + KL +
Sbjct: 171 NEYGRDEEDHE---NRNRTAD-KGGILDELDDFIEDDEFSDEDDETRQRRI--QEKKLLR 224
Query: 311 QEKDKKPFLILEGDGIDEEQADVKYIVTETNATEDWNLKIENQSEKTGSNSDDNHELTVN 490
++ K+P I G+ ++ D Y + DW L+IEN+ + G+++++ E ++
Sbjct: 225 EQSIKQPTQIT---GLSSDKIDEMYDIFGDGHDYDWALEIENEELENGNDNNEAEEEEID 281
Query: 491 QKS 499
+++
Sbjct: 282 EET 284
>sp|P53843|YN05_YEAST Hypothetical 34.5 kDa protein in PIK1-POL2 intergenic region
Length = 298
Score = 38.5 bits (88), Expect = 0.025
Identities = 32/134 (23%), Positives = 59/134 (44%), Gaps = 8/134 (5%)
Frame = +2
Query: 197 EDIKQVVVNEVKDTFSF-------SIKSESCKVDPIFSDKTKLFKQEKDKKPFLILEGDG 355
E++ + + E+ T+ S+ S S + FSD + + ++KP L L+ D
Sbjct: 166 EELVDLYLKEIAKTYDVPYSKLENSLSSSSSNISSDFSDPSGDIEDNDEEKPILALDND- 224
Query: 356 IDEEQADVKYIVTETNATEDWNLKIENQSEKTGSNSDD-NHELTVNQKSKLKNYEQLSSK 532
D + AD K+ +T +K NS++ +EL + + K + E+ S+
Sbjct: 225 -DNDNADAKHPIT---------------VKKPRQNSENIKNELKIPKDIKKEVIEKKQSE 268
Query: 533 INSTKESNGKEKRN 574
+TK KE+ N
Sbjct: 269 KKTTKRKTKKEQEN 282
>sp|Q99L45|IF2B_MOUSE Eukaryotic translation initiation factor 2 subunit 2 (Eukaryotic
translation initiation factor 2 beta subunit)
(eIF-2-beta)
Length = 331
Score = 38.1 bits (87), Expect = 0.032
Identities = 26/85 (30%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 275 DPIFSDKTKLFKQEKDKKPFLI-LEGDGIDEEQADVKYIVTETNATEDWNLKIENQSEKT 451
D + D T K++K KKPF++ EGD EE + E TE+ ++ + + +
Sbjct: 4 DEMIFDPTMSKKKKKKKKPFMLDEEGDAQTEETQPSETKEVEPEPTEEKDVDADEEDSRK 63
Query: 452 GSNSDDNHELT-VNQKSKLKNYEQL 523
SDD +L NQK K K +++
Sbjct: 64 KDASDDLDDLNFFNQKKKKKKTKKI 88
>sp|Q38433|ALT_BPT6 NAD--protein ADP-ribosyltransferase (Alt protein)
Length = 698
Score = 38.1 bits (87), Expect = 0.032
Identities = 23/69 (33%), Positives = 35/69 (50%)
Frame = +2
Query: 50 LIPTSIEMKLAIGETESTINHNKLTSSWTTNEYEDEDKLHNSIYNSNKTEDIKQVVVNEV 229
LIP + EMKL I S K+ + T NE + K + + +E IK V++N +
Sbjct: 269 LIPEAEEMKLGINSLASKTKAAKIIAEGTANELHYDYKFFSKSEVNEVSEKIKDVILNAI 328
Query: 230 KDTFSFSIK 256
K+ + SIK
Sbjct: 329 KNEPTTSIK 337
>sp|P39922|MYS3_HYDAT Myosin heavy chain, clone 203
Length = 539
Score = 36.6 bits (83), Expect = 0.094
Identities = 32/152 (21%), Positives = 67/152 (44%), Gaps = 2/152 (1%)
Frame = +2
Query: 161 KLHNSIYNSNKTEDIKQVVVNEVKDTFSFSIKSESCKVDPIFSDKTKLFKQEKDKKPFLI 340
+L N +K D + + +T S + +E VD D+T+ + +DK L
Sbjct: 166 RLETEKQNRDKQIDTLNEDIRKQDETIS-KMNAEKKHVDEELKDRTEQLQAAEDKCNNLN 224
Query: 341 LEGDGIDEEQADVKYIVTETNATEDWNLKIENQSEKTGSNSDDNHELTVNQKSKLKNYEQ 520
+ ++ + ++ I + +D +K+E + +K S+ DN + +++LK +
Sbjct: 225 KTKNKLE---SSIREIEQDLKKEKDSKMKLEKEKKKVESDLKDNRDKLSETETRLKETQD 281
Query: 521 LSSKINSTKE--SNGKEKRNVPVGRVQPMIKE 610
L +K + N KE + ++Q I+E
Sbjct: 282 LVTKREKSISDLENAKEGLESQISQLQRKIQE 313
>sp|Q38424|ALT_BPT2 NAD--protein ADP-ribosyltransferase (Alt protein)
Length = 698
Score = 36.2 bits (82), Expect = 0.12
Identities = 22/69 (31%), Positives = 33/69 (47%)
Frame = +2
Query: 50 LIPTSIEMKLAIGETESTINHNKLTSSWTTNEYEDEDKLHNSIYNSNKTEDIKQVVVNEV 229
LIP + EMK+ I S K+ + T NE + K + +E IK V+ N +
Sbjct: 269 LIPEAEEMKIGINSLASKTKAAKIIAEGTANELHYDYKFFSKSEVDEVSEKIKDVIFNAI 328
Query: 230 KDTFSFSIK 256
K+ + SIK
Sbjct: 329 KNEPTTSIK 337
>sp|P08116|PVA_PLAFA Processed variable antigen
Length = 224
Score = 35.4 bits (80), Expect = 0.21
Identities = 40/169 (23%), Positives = 76/169 (44%), Gaps = 4/169 (2%)
Frame = +2
Query: 86 GETESTINHNKLTSSWTTNEYEDEDKLHNSIYNSNKTEDIKQVVVNEVKDTFSFSIKSES 265
GE++ T + S T E ++ + + S +T + K+ E K+T + +++
Sbjct: 57 GESKETGESKETGESKETGESKETGESKET-GESKETGESKET--GESKETRIYE-ETKY 112
Query: 266 CKVDPIF--SDKTKLFKQEKDKKPFLILEGDGIDE--EQADVKYIVTETNATEDWNLKIE 433
K+ F ++ K+ ++ KD+ EG+ + E ++ + +E+ T+ K E
Sbjct: 113 NKITSEFRETENVKITEESKDR------EGNKVSGPYENSENSNVTSESEETKKLAEKEE 166
Query: 434 NQSEKTGSNSDDNHELTVNQKSKLKNYEQLSSKINSTKESNGKEKRNVP 580
N+ EK G N +D KL E+ N TKES+ + K + P
Sbjct: 167 NEGEKLGENVNDGASENSEDPKKLTEQEE-----NGTKESSEETKDDKP 210
>sp|Q02455|MLP1_YEAST Protein MLP1 (Myosin-like protein 1)
Length = 1875
Score = 35.0 bits (79), Expect = 0.27
Identities = 44/191 (23%), Positives = 81/191 (42%), Gaps = 23/191 (12%)
Frame = +2
Query: 17 KSFITNAVAESLIPTSIEMKLAIGETESTINHNKLTSSWTTNEYEDEDKLHNSIYNS--N 190
+S IT+ ++ ++ K+ E E + +L + +E ++D L + S N
Sbjct: 856 ESVITSVDSKQTDIEKLQYKVKSLEKEIEEDKIRLHTYNVMDETINDDSLRKELEKSKIN 915
Query: 191 KTEDIKQVV-VNEVKDTFSFSIKSESCKVDPIFSDKTKLFKQEKDKKPF----------- 334
T+ Q+ ++ +T S S++ + K+D F D T K D+K
Sbjct: 916 LTDAYSQIKEYKDLYETTSQSLQQTNSKLDESFKDFTNQIKNLTDEKTSLEDKISLLKEQ 975
Query: 335 -------LILEGDGIDEEQADVK--YIVTETNATEDWNLKIENQSEKTGSNSDDNHELTV 487
L L+ G+++E+AD K + + N E +K E +S K +D + T+
Sbjct: 976 MFNLNNELDLQKKGMEKEKADFKKRISILQNNNKEVEAVKSEYES-KLSKIQNDLDQQTI 1034
Query: 488 NQKSKLKNYEQ 520
+ NYEQ
Sbjct: 1035 YANTAQNNYEQ 1045
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,847,385
Number of Sequences: 369166
Number of extensions: 1518674
Number of successful extensions: 4991
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4715
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4975
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8694177530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)