Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_N06
(510 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST... 65 9e-11
sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST... 61 1e-09
sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST... 60 2e-09
sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-si... 59 7e-09
sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST... 57 3e-08
sp|O73888|PTGD2_CHICK Glutathione-requiring prostaglandin D... 50 4e-06
sp|O18598|GST1_BLAGE Glutathione S-transferase (GST class-s... 47 2e-05
sp|P91253|GST7_CAEEL Probable glutathione S-transferase 7 (... 44 2e-04
sp|P91254|GST8_CAEEL Probable glutathione S-transferase 8 (... 44 3e-04
sp|P30112|GST26_FASHE Glutathione S-transferase 26 kDa 51 (... 42 8e-04
>sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST 28) (SM28 antigen)
(Protective 28 kDa antigen) (GST class-mu)
Length = 211
Score = 65.1 bits (157), Expect = 9e-11
Identities = 39/127 (30%), Positives = 68/127 (53%), Gaps = 4/127 (3%)
Frame = +3
Query: 84 YFVGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGF 263
+ +GE D+EYY +E+ IGQ DV +K P+E+KE + E + L +
Sbjct: 83 HMMGETDEEYYSVEKLIGQAEDVEHEYHKTLMKPQEEKEKITKEILNGKVPVLLNMICES 142
Query: 264 IKES--GFFVGEKITLAELFFVVLTDYLEKLPK--LYDDFPKLKELRERVLKANTDVEDW 431
+K S VG+K+TLA+L + + D++ L K L +P++ + RE +L ++ + +
Sbjct: 143 LKGSTGKLAVGDKVTLADLVLIAVIDHVTDLDKGFLTGKYPEIHKHRENLLASSPRLAKY 202
Query: 432 VKTRPVT 452
+ RP T
Sbjct: 203 LSNRPAT 209
Score = 33.9 bits (76), Expect = 0.23
Identities = 14/24 (58%), Positives = 20/24 (83%)
Frame = -1
Query: 90 QSMAIARAIARKYGFMGEDDKEYY 19
+S+AIAR +A+K+ MGE D+EYY
Sbjct: 70 ESLAIARYMAKKHHMMGETDEEYY 93
>sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST 28) (SJ28 antigen) (GST
class-mu)
Length = 206
Score = 61.2 bits (147), Expect = 1e-09
Identities = 37/125 (29%), Positives = 67/125 (53%), Gaps = 4/125 (3%)
Frame = +3
Query: 90 VGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIK 269
+G+ D EYY+IE+ IGQ+ DV +K P E+KE + E + L+++ +K
Sbjct: 80 MGDTDDEYYIIEKMIGQVEDVESEYHKTLIKPPEEKEKISKEILNGKVPILLQAICETLK 139
Query: 270 ES--GFFVGEKITLAELFFVVLTDYLEKLPK--LYDDFPKLKELRERVLKANTDVEDWVK 437
ES VG+K+TLA++ + D++ L K L +P++ + R+ +L + + ++
Sbjct: 140 ESTGNLTVGDKVTLADVVLIASIDHITDLDKEFLTGKYPEIHKHRKHLLATSPKLAKYLS 199
Query: 438 TRPVT 452
R T
Sbjct: 200 ERHAT 204
Score = 36.2 bits (82), Expect = 0.046
Identities = 15/28 (53%), Positives = 21/28 (75%)
Frame = -1
Query: 90 QSMAIARAIARKYGFMGEDDKEYYLTNR 7
+S+AIAR IARK+ MG+ D EYY+ +
Sbjct: 65 ESLAIARFIARKHNMMGDTDDEYYIIEK 92
>sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
Length = 211
Score = 60.5 bits (145), Expect = 2e-09
Identities = 37/127 (29%), Positives = 68/127 (53%), Gaps = 4/127 (3%)
Frame = +3
Query: 84 YFVGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGF 263
+ +GE D+EYY +E+ IGQ+ D+ +K PEE+K+ + E + L +
Sbjct: 83 HMMGETDEEYYNVEKLIGQVEDLEHEYHKTLMKPEEEKQKITKEILNGKVPVLLDIICES 142
Query: 264 IKES--GFFVGEKITLAELFFVVLTDYLEKLPK--LYDDFPKLKELRERVLKANTDVEDW 431
+K S VG+K+TLA+L + + D++ L K L +P++ + RE +L ++ + +
Sbjct: 143 LKASTGKLAVGDKVTLADLVLIAVIDHVTDLDKEFLTGKYPEIHKHRENLLASSPRLAKY 202
Query: 432 VKTRPVT 452
+ R T
Sbjct: 203 LSDRAAT 209
Score = 33.9 bits (76), Expect = 0.23
Identities = 14/24 (58%), Positives = 20/24 (83%)
Frame = -1
Query: 90 QSMAIARAIARKYGFMGEDDKEYY 19
+S+AIAR +A+K+ MGE D+EYY
Sbjct: 70 ESLAIARYMAKKHHMMGETDEEYY 93
>sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-sigma)
Length = 203
Score = 58.9 bits (141), Expect = 7e-09
Identities = 42/126 (33%), Positives = 65/126 (51%), Gaps = 5/126 (3%)
Frame = +3
Query: 93 GEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGFIKE 272
G+ E Y ++ + D+ + KI FAPE KE ++ + + R LEG +
Sbjct: 79 GKTSLEKYRVDEITETLQDIFNDVVKIKFAPEAAKEAVQQNYEKSCKR-LAPFLEGLLVS 137
Query: 273 SG----FFVGEKITLAELF-FVVLTDYLEKLPKLYDDFPKLKELRERVLKANTDVEDWVK 437
+G FFVG +TLA+L +V L L+ P+L D PK+ LR+RV + + ++K
Sbjct: 138 NGGGDGFFVGNSMTLADLHCYVALEVPLKHTPELLKDCPKIVALRKRVAEC-PKIAAYLK 196
Query: 438 TRPVTD 455
RPV D
Sbjct: 197 KRPVRD 202
>sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
Length = 211
Score = 56.6 bits (135), Expect = 3e-08
Identities = 36/127 (28%), Positives = 66/127 (51%), Gaps = 4/127 (3%)
Frame = +3
Query: 84 YFVGEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEEKKEDLRTEFVTTSGRDNLKSLEGF 263
+ +G ++EYY +E+ IGQ D+ YK PEE+K+ + E + L +
Sbjct: 83 HMMGGTEEEYYNVEKLIGQAEDLEHEYYKTLMKPEEEKQKIIKEILNGKVPVLLDIICES 142
Query: 264 IKES--GFFVGEKITLAELFFVVLTDYLEKLPK--LYDDFPKLKELRERVLKANTDVEDW 431
+K S VG+K+TLA+L + + D++ L K L +P++ + RE +L ++ + +
Sbjct: 143 LKASTGKLAVGDKVTLADLVLIAVIDHVTDLDKEFLTGKYPEIHKHRENLLASSPRLAKY 202
Query: 432 VKTRPVT 452
+ R T
Sbjct: 203 LSDRAAT 209
Score = 29.6 bits (65), Expect = 4.3
Identities = 12/24 (50%), Positives = 19/24 (79%)
Frame = -1
Query: 90 QSMAIARAIARKYGFMGEDDKEYY 19
+S+AIAR +A+K+ MG ++EYY
Sbjct: 70 ESLAIARYMAKKHHMMGGTEEEYY 93
>sp|O73888|PTGD2_CHICK Glutathione-requiring prostaglandin D synthase
(Glutathione-dependent PGD synthetase) (Prostaglandin-H2
D-isomerase) (Hematopoietic prostaglandin D synthase)
(H-PGDS)
Length = 199
Score = 49.7 bits (117), Expect = 4e-06
Identities = 34/117 (29%), Positives = 64/117 (54%), Gaps = 6/117 (5%)
Frame = +3
Query: 120 IERAIGQ-MVDVLEGMYKIYFAPEEKKEDLR----TEFVTTSGRDNLKSLEGFIKESGFF 284
+E+A+ +VD ++ + + F EK +D++ + +T + LK L+ F+ + +F
Sbjct: 83 VEQALADAIVDTIDD-FMMLFPWAEKNQDVKEKAFNDILTNKAPELLKDLDTFLGDKKWF 141
Query: 285 VGEKITLAELFF-VVLTDYLEKLPKLYDDFPKLKELRERVLKANTDVEDWVKTRPVT 452
VG+ +T A+ ++ V T L L D +P+L LR+RV +A + W++ RP T
Sbjct: 142 VGKSVTWADFYWDVCSTTLLSYKADLADKYPRLLALRDRV-EALPAIAAWIQKRPKT 197
>sp|O18598|GST1_BLAGE Glutathione S-transferase (GST class-sigma) (Major allergen Bla g
5)
Length = 204
Score = 47.4 bits (111), Expect = 2e-05
Identities = 38/131 (29%), Positives = 61/131 (46%), Gaps = 9/131 (6%)
Frame = +3
Query: 93 GEDDKEYYLIERAIGQMVDVLEGMYKIYFAPEE-----KKEDLRTEFVT--TSGRDNLKS 251
G+DD E I+ + + D + ++ +E K + L+ E + T D +
Sbjct: 80 GKDDWENLEIDMIVDTISDFRAAIANYHYDADENSKQKKWDPLKKETIPYYTKKFDEVVK 139
Query: 252 LEGFIKESGFFVGEKITLAELFFVVLTDYLEKLPK--LYDDFPKLKELRERVLKANTDVE 425
G G+ K+T A+ +FV + DYL + K L + P LK LRE+VL ++
Sbjct: 140 ANG-----GYLAAGKLTWADFYFVAILDYLNHMAKEDLVANQPNLKALREKVLGLPA-IK 193
Query: 426 DWVKTRPVTDM 458
WV RP TD+
Sbjct: 194 AWVAKRPPTDL 204
>sp|P91253|GST7_CAEEL Probable glutathione S-transferase 7 (GST class-sigma)
Length = 206
Score = 43.9 bits (102), Expect = 2e-04
Identities = 23/61 (37%), Positives = 37/61 (60%), Gaps = 1/61 (1%)
Frame = +3
Query: 273 SGFFVGEKITLAELFFVVLT-DYLEKLPKLYDDFPKLKELRERVLKANTDVEDWVKTRPV 449
SG+ VG+ +T +L T D L L D+FP+ K +E+V +N +++ W++TRPV
Sbjct: 145 SGYLVGDSLTFVDLLVAQHTADLLAANAALLDEFPQFKAHQEKV-HSNANIKKWLETRPV 203
Query: 450 T 452
T
Sbjct: 204 T 204
>sp|P91254|GST8_CAEEL Probable glutathione S-transferase 8 (GST class-sigma)
Length = 206
Score = 43.5 bits (101), Expect = 3e-04
Identities = 22/61 (36%), Positives = 37/61 (60%), Gaps = 1/61 (1%)
Frame = +3
Query: 273 SGFFVGEKITLAELFFVVLT-DYLEKLPKLYDDFPKLKELRERVLKANTDVEDWVKTRPV 449
SG+ VG+ +T +L T D L P L ++FP+ K +E+V +N +++ W++TRP
Sbjct: 145 SGYLVGDSLTFVDLLIAQHTADILSTDPALLEEFPQFKAHQEKV-HSNANIKKWLETRPE 203
Query: 450 T 452
T
Sbjct: 204 T 204
>sp|P30112|GST26_FASHE Glutathione S-transferase 26 kDa 51 (GST51) (FH51) (GST class-mu)
Length = 218
Score = 42.0 bits (97), Expect = 8e-04
Identities = 33/124 (26%), Positives = 55/124 (44%), Gaps = 8/124 (6%)
Frame = +3
Query: 90 VGEDDKEYYLIERAIGQMVDVLEGMYKIYFAP--EEKKEDLRTEFVTTSGRDNLKSLEGF 263
+G +E I G +D+ G ++ + P EE K D E TT LK F
Sbjct: 82 LGTTPEERARISMIEGAAMDLRMGFVRVCYNPKFEEVKGDYLKELPTT-----LKMWSNF 136
Query: 264 IKESGFFVGEKITLAELFFVVLTDYLEKL-PKLYDDFPKLKELRERV-----LKANTDVE 425
+ + + G ++ + D + L P+ +DFPKLKE + R+ +KA + E
Sbjct: 137 LGDRHYLTGSPVSHVDFMVYEALDCIRYLAPQCLEDFPKLKEFKSRIEDLPKIKAYMESE 196
Query: 426 DWVK 437
++K
Sbjct: 197 KFIK 200
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58,916,220
Number of Sequences: 369166
Number of extensions: 1174687
Number of successful extensions: 3913
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3797
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3908
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3255600150
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)