Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_M16
(771 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q04820|MDHC_ECHGR Malate dehydrogenase, cytoplasmic 300 3e-81
sp|P11708|MDHC_PIG Malate dehydrogenase, cytoplasmic 298 8e-81
sp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic 298 1e-80
sp|P14152|MDHC_MOUSE Malate dehydrogenase, cytoplasmic 298 1e-80
sp|Q83C87|MDH_COXBU Malate dehydrogenase 258 1e-68
sp|P57106|MDHD_ARATH Malate dehydrogenase, cytoplasmic 2 250 3e-66
sp|P93819|MDHC1_ARATH Malate dehydrogenase, cytoplasmic 1 250 3e-66
sp|O24047|MDHC_MESCR Malate dehydrogenase, cytoplasmic 249 6e-66
sp|Q82WB9|MDH_NITEU Malate dehydrogenase 248 2e-65
sp|O48905|MDHC_MEDSA Malate dehydrogenase, cytoplasmic 246 5e-65
>sp|Q04820|MDHC_ECHGR Malate dehydrogenase, cytoplasmic
Length = 332
Score = 300 bits (768), Expect = 3e-81
Identities = 152/236 (64%), Positives = 181/236 (76%)
Frame = +2
Query: 11 MTPPITVVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDC 190
M P+ V++TGAAGQI Y L + VA+GN+FG+ Q I LHLLDIP+ L+G++MEL+DC
Sbjct: 1 MPGPLRVLITGAAGQIAYNLSNMVANGNLFGKDQQIILHLLDIPEAKTVLDGVVMELQDC 60
Query: 191 AFPQLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYA 370
AF L + T L+EAF D+D ALMVGAMPRK+GMER+DLL +NV IFK QGEALDKYA
Sbjct: 61 AFTVLAGIVPTHCLKEAFTDIDVALMVGAMPRKQGMERRDLLSSNVKIFKEQGEALDKYA 120
Query: 371 KKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRR 550
KKTV+V VVGNPANTNCLIMSKYAPSIPKENF+ALTRLDHNRAI Q+A KAGVP V+
Sbjct: 121 KKTVKVLVVGNPANTNCLIMSKYAPSIPKENFTALTRLDHNRAIYQVAAKAGVPNTCVKN 180
Query: 551 CIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVI 718
IWGNHSN Q+PD H +V DGK+ + + DE W+K F+ VQ RGAAVI
Sbjct: 181 VCIWGNHSNKQFPDLSHAVVT-KDGKQHPAKELINDEKWVKEVFIPCVQNRGAAVI 235
>sp|P11708|MDHC_PIG Malate dehydrogenase, cytoplasmic
Length = 334
Score = 298 bits (764), Expect = 8e-81
Identities = 154/236 (65%), Positives = 185/236 (78%)
Frame = +2
Query: 11 MTPPITVVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDC 190
M+ PI V++TGAAGQI Y L++S+ +G+VFG+ QPI L LLDI M+ L+G++MEL+DC
Sbjct: 1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDC 60
Query: 191 AFPQLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYA 370
A P L V AT + AF D+D A++VG+MPR++GMERKDLLKANV IFK QG ALDKYA
Sbjct: 61 ALPLLKDVIATDKEEIAFKDLDVAILVGSMPRRDGMERKDLLKANVKIFKCQGAALDKYA 120
Query: 371 KKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRR 550
KK+V+V VVGNPANTNCL SK APSIPKENFS LTRLDHNRA AQIA K GV D V+
Sbjct: 121 KKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTSDDVKN 180
Query: 551 CIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVI 718
IIWGNHS+TQYPD +H VK KEV V +AVKD++WLK +F+ TVQ RGAAVI
Sbjct: 181 VIIWGNHSSTQYPDVNHAKVK-LQAKEVGVYEAVKDDSWLKGEFITTVQQRGAAVI 235
>sp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic
Length = 334
Score = 298 bits (763), Expect = 1e-80
Identities = 154/236 (65%), Positives = 185/236 (78%)
Frame = +2
Query: 11 MTPPITVVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDC 190
M+ PI V++TGAAGQI Y L++S+ +G+VFG+ QPI L LLDI M+ L+G++MEL+DC
Sbjct: 1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDC 60
Query: 191 AFPQLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYA 370
A P L V AT AF D+D A++VG+MPR+EGMERKDLLKANV IFK QG ALDKYA
Sbjct: 61 ALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYA 120
Query: 371 KKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRR 550
KK+V+V VVGNPANTNCL SK APSIPKENFS LTRLDHNRA AQIA K GV + V+
Sbjct: 121 KKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKN 180
Query: 551 CIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVI 718
IIWGNHS+TQYPD +H VK GKEV V +A+KD++WLK +F+ TVQ RGAAVI
Sbjct: 181 VIIWGNHSSTQYPDVNHAKVK-LQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVI 235
>sp|P14152|MDHC_MOUSE Malate dehydrogenase, cytoplasmic
Length = 334
Score = 298 bits (762), Expect = 1e-80
Identities = 153/236 (64%), Positives = 186/236 (78%)
Frame = +2
Query: 11 MTPPITVVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDC 190
M+ PI V++TGAAGQI Y L++S+ +G+VFG+ QPI L LLDI M+ L+G++MEL+DC
Sbjct: 1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDC 60
Query: 191 AFPQLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYA 370
A P L V AT + AF D+D A++VG+MPR+EGMERKDLLKANV IFK QG AL+KYA
Sbjct: 61 ALPLLQDVIATDKEEIAFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGTALEKYA 120
Query: 371 KKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRR 550
KK+V+V VVGNPANTNCL SK APSIPKENFS LTRLDHNRA +QIA K GV D V+
Sbjct: 121 KKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKN 180
Query: 551 CIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVI 718
IIWGNHS+TQYPD +H VK GKEV V +A+KD++WLK +F+ TVQ RGAAVI
Sbjct: 181 VIIWGNHSSTQYPDVNHAKVK-LQGKEVGVYEALKDDSWLKGEFITTVQQRGAAVI 235
>sp|Q83C87|MDH_COXBU Malate dehydrogenase
Length = 328
Score = 258 bits (659), Expect = 1e-68
Identities = 129/236 (54%), Positives = 167/236 (70%)
Frame = +2
Query: 11 MTPPITVVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDC 190
M + V +TGAAGQIGY L+ +ASG FG ++LHLL+I +P+L G++MELEDC
Sbjct: 1 MAKHVKVAVTGAAGQIGYALLFRLASGQAFGLDTTVDLHLLEIEPALPALKGVVMELEDC 60
Query: 191 AFPQLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYA 370
AFP L ++ T+D + AF+DV++AL+VGA PRK GMERKDLL+ N +IF QG+A+++ A
Sbjct: 61 AFPLLRNMVVTSDPRVAFNDVNWALLVGAAPRKAGMERKDLLEKNGSIFAGQGKAINENA 120
Query: 371 KKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRR 550
VR+ VVGNP NTNCLI AP IPK+ F A+TRLD NRAI Q+A KAGV V+
Sbjct: 121 ASDVRIFVVGNPCNTNCLIAMNNAPDIPKDRFYAMTRLDQNRAIGQLALKAGVDVPSVKN 180
Query: 551 CIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVI 718
IIWGNHS+TQYPDF+H + G EV ++D+ WL NDF+ +Q RGAAVI
Sbjct: 181 MIIWGNHSSTQYPDFYHATIDGKPATEV-----IRDKNWLLNDFISVIQQRGAAVI 231
>sp|P57106|MDHD_ARATH Malate dehydrogenase, cytoplasmic 2
Length = 332
Score = 250 bits (639), Expect = 3e-66
Identities = 126/233 (54%), Positives = 163/233 (69%)
Frame = +2
Query: 20 PITVVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 199
P+ V++TGAAGQIGY LV +A G + G QP+ LH+LDIP +LNG+ MEL D AFP
Sbjct: 5 PVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPFAAEALNGVKMELVDAAFP 64
Query: 200 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 379
L V AT D EA V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+K+A
Sbjct: 65 LLKGVVATTDAVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAPN 124
Query: 380 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 559
+V VV NPANTN LI+ ++APSIP++N + LTRLDHNRA+ Q++ + VP V+ II
Sbjct: 125 CKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQVSERLSVPVSDVKNVII 184
Query: 560 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVI 718
WGNHS+TQYPD +H VK G E V + VK++ WL +F+ TVQ RGAA+I
Sbjct: 185 WGNHSSTQYPDVNHATVKTSVG-EKPVRELVKNDEWLNGEFISTVQQRGAAII 236
>sp|P93819|MDHC1_ARATH Malate dehydrogenase, cytoplasmic 1
Length = 332
Score = 250 bits (638), Expect = 3e-66
Identities = 126/233 (54%), Positives = 162/233 (69%)
Frame = +2
Query: 20 PITVVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 199
P+ V++TGAAGQIGY LV +A G + G QP+ LH+LDIP +LNG+ MEL D AFP
Sbjct: 5 PVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELIDAAFP 64
Query: 200 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 379
L V AT D E V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+K+A
Sbjct: 65 LLKGVVATTDAVEGCTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKHAAPN 124
Query: 380 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 559
+V VV NPANTN LI+ ++APSIP++N S LTRLDHNRA+ QI+ + VP V+ II
Sbjct: 125 CKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLSVPVSDVKNVII 184
Query: 560 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVI 718
WGNHS++QYPD +H V+ G E V + VKD+ WL +F+ TVQ RGAA+I
Sbjct: 185 WGNHSSSQYPDVNHAKVQTSSG-EKPVRELVKDDAWLDGEFISTVQQRGAAII 236
>sp|O24047|MDHC_MESCR Malate dehydrogenase, cytoplasmic
Length = 332
Score = 249 bits (636), Expect = 6e-66
Identities = 125/233 (53%), Positives = 162/233 (69%)
Frame = +2
Query: 20 PITVVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 199
P+ V++TGAAGQIGY LV +A G + G +QP+ LH+LDIP +LNG+ MEL D AFP
Sbjct: 5 PLRVLVTGAAGQIGYALVPMIARGIMLGANQPVILHMLDIPPAAEALNGVKMELVDAAFP 64
Query: 200 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 379
L V AT D EA V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+++A
Sbjct: 65 LLKGVVATTDAAEACKGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPN 124
Query: 380 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 559
+V VV NPANTN LI+ ++APSIP++N S LTRLDHNRA+ QI+ + V V+ II
Sbjct: 125 CKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVKNVII 184
Query: 560 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVI 718
WGNHS+TQYPD +H VK G + V + V D+ WL +F+ TVQ RGAA+I
Sbjct: 185 WGNHSSTQYPDVNHATVK-TQGVDKPVRELVADDAWLNGEFITTVQQRGAAII 236
>sp|Q82WB9|MDH_NITEU Malate dehydrogenase
Length = 327
Score = 248 bits (632), Expect = 2e-65
Identities = 119/236 (50%), Positives = 161/236 (68%)
Frame = +2
Query: 11 MTPPITVVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDC 190
M+ PI + +TGAAGQI Y L+ +A+G++ G QP+ L LLDIP+ L+G++MEL+DC
Sbjct: 1 MSSPIRIAVTGAAGQISYSLLFRIAAGDMLGSSQPVILQLLDIPESGKVLDGVLMELQDC 60
Query: 191 AFPQLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYA 370
AFP L + T D AF D A++VGA PR +GMERKDLL+ N IF+ QG AL++
Sbjct: 61 AFPLLTDIIVTHDPMIAFDQADIAILVGARPRGKGMERKDLLQTNGEIFREQGRALNQVV 120
Query: 371 KKTVRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRR 550
K+ ++ VVGNPANTN LI K AP + ENFS + RLDHNRA++Q+A K P H+R+
Sbjct: 121 KRDAKILVVGNPANTNTLITMKNAPDLSPENFSGMLRLDHNRALSQVAMKLNQPVSHIRK 180
Query: 551 CIIWGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVI 718
I+WGNHS+TQ+PD H + V+D +KD+TW++N F+ TVQ RGA VI
Sbjct: 181 MIVWGNHSSTQFPDLSHAEI-----DHQKVIDLIKDQTWVENSFIPTVQNRGAVVI 231
>sp|O48905|MDHC_MEDSA Malate dehydrogenase, cytoplasmic
Length = 332
Score = 246 bits (628), Expect = 5e-65
Identities = 126/233 (54%), Positives = 158/233 (67%)
Frame = +2
Query: 20 PITVVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 199
P+ V++TGAAGQIGY LV +A G + G QP+ LH+LDI SLNG+ MEL D AFP
Sbjct: 5 PVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIAPAAESLNGVKMELVDAAFP 64
Query: 200 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 379
L V AT D+ EA V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+K+A
Sbjct: 65 LLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAAN 124
Query: 380 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 559
+V VV NPANTN LI+ ++APSIP+ N S LTRLDHNRA+ QI+ + V V+ II
Sbjct: 125 CKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERLNVQVSDVKNVII 184
Query: 560 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVI 718
WGNHS+TQYPD +H V G E V V D+ WL +F+ TVQ RGAA+I
Sbjct: 185 WGNHSSTQYPDVNHATVNTPAG-EKPVRQLVSDDAWLNGEFISTVQQRGAAII 236
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,562,453
Number of Sequences: 369166
Number of extensions: 1724740
Number of successful extensions: 4972
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4778
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4904
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7163732800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)