Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_M12
(482 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 76 4e-14
sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20 75 6e-14
sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 ... 75 6e-14
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 75 8e-14
sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p... 74 2e-13
sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 ... 73 3e-13
sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinas... 73 3e-13
sp|Q12469|SKM1_YEAST Serine/threonine-protein kinase SKM1 (... 73 4e-13
sp|Q64303|PAK2_RAT Serine/threonine-protein kinase PAK 2 (p... 73 4e-13
sp|Q8CIN4|PAK2_MOUSE Serine/threonine-protein kinase PAK 2 ... 73 4e-13
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 76.3 bits (186), Expect = 4e-14
Identities = 60/161 (37%), Positives = 81/161 (50%), Gaps = 1/161 (0%)
Frame = +3
Query: 3 KLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVEL 182
KL F ++K + ++ T A+S GT W APEVI G+ SADIWS G ++E+
Sbjct: 212 KLADFGASKQVAELATMTG----AKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEM 267
Query: 183 ITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLM 359
+TGKAP AI+ +GT K SDA K F+ CL P RPT +L+
Sbjct: 268 VTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDA-KDFLLKCLQEVPNLRPTASELL 326
Query: 360 KHEFLNSAMEAD*FT*RVDIFSWIN*NNTYMLPK*INNCSS 482
KH F+ + T D+ S +N N + LP INN S
Sbjct: 327 KHPFVMGKHKESAST---DLGSVLN-NLSTPLPLQINNTKS 363
>sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20
Length = 939
Score = 75.5 bits (184), Expect = 6e-14
Identities = 36/103 (34%), Positives = 57/103 (55%)
Frame = +3
Query: 87 GTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFA 266
GT W APEV++ + YG DIWS G +++E+I G+ P +A+Y + T +
Sbjct: 776 GTPYWMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLK 835
Query: 267 YPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSAMEAD 395
P S +LK F+D CL EP +R + +L+ E++ EA+
Sbjct: 836 EPENLSSSLKKFLDWCLCVEPEDRASATELLHDEYITEIAEAN 878
>sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK)
Length = 545
Score = 75.5 bits (184), Expect = 6e-14
Identities = 36/99 (36%), Positives = 54/99 (54%)
Frame = +3
Query: 87 GTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFA 266
GT W APEV+ + YG DIWS G + +E+I G+ P +A+Y + T E
Sbjct: 426 GTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQ 485
Query: 267 YPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 383
P K S + F++ CLD + R + K+L++H+FL A
Sbjct: 486 NPEKLSAIFRDFLNRCLDMDVEKRGSAKELLQHQFLKIA 524
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 75.1 bits (183), Expect = 8e-14
Identities = 50/138 (36%), Positives = 71/138 (51%), Gaps = 1/138 (0%)
Frame = +3
Query: 72 AESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQ 248
A+S GT W APEVI G+ SADIWS G ++E++TGKAP AI+ +GT
Sbjct: 230 AKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTT 289
Query: 249 KKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSAMEAD*FT*RVDIFSW 428
K P S F+ CL EP RPT +L+KH F+ + + D+ S+
Sbjct: 290 KS-HPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKE---SASKDLTSF 345
Query: 429 IN*NNTYMLPK*INNCSS 482
++ N+ LP + N +S
Sbjct: 346 MD-NSCSPLPSELTNITS 362
>sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P68-PAK) (Alpha-PAK) (Protein kinase MUK2)
Length = 544
Score = 73.9 bits (180), Expect = 2e-13
Identities = 35/99 (35%), Positives = 54/99 (54%)
Frame = +3
Query: 87 GTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFA 266
GT W APEV+ + YG DIWS G + +E+I G+ P +A+Y + T E
Sbjct: 425 GTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQ 484
Query: 267 YPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 383
P K S + F++ CL+ + R + K+L++H+FL A
Sbjct: 485 NPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIA 523
>sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK) (CDC42/RAC effector kinase
PAK-A)
Length = 545
Score = 73.2 bits (178), Expect = 3e-13
Identities = 35/99 (35%), Positives = 53/99 (53%)
Frame = +3
Query: 87 GTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFA 266
GT W APEV+ + YG DIWS G + +E+I G+ P +A+Y + T E
Sbjct: 426 GTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQ 485
Query: 267 YPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 383
P K S + F+ CL+ + R + K+L++H+FL A
Sbjct: 486 NPEKLSAIFRDFLQCCLEMDVEKRGSAKELLQHQFLKIA 524
>sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4) (MAP three kinase 1)
Length = 1607
Score = 73.2 bits (178), Expect = 3e-13
Identities = 42/103 (40%), Positives = 60/103 (58%), Gaps = 4/103 (3%)
Frame = +3
Query: 78 SNHGTANWTAPEVIN---GEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGT 245
S GTA + APEVI GEG+G +ADIWS G V++E++TGK P + +FQ +Y++G
Sbjct: 1500 STLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGM 1559
Query: 246 QKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 374
K P + S K F+ CL+ +P R T L+ H F+
Sbjct: 1560 GHKPPI--PERLSPEGKDFLSHCLESDPKMRWTASQLLDHSFV 1600
>sp|Q12469|SKM1_YEAST Serine/threonine-protein kinase SKM1 (Protein kinase 75490 D)
Length = 655
Score = 72.8 bits (177), Expect = 4e-13
Identities = 33/99 (33%), Positives = 53/99 (53%)
Frame = +3
Query: 87 GTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFA 266
GT W APE++N +GY D+WS G +++E+I G+ P +A+Y + +
Sbjct: 544 GTPYWMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLNEDPLKALYLIANNGSPKLR 603
Query: 267 YPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 383
+P S K F+D CL +R +V+ L+ EFL+ A
Sbjct: 604 HPESVSKQTKQFLDACLQVNVESRASVRKLLTFEFLSMA 642
>sp|Q64303|PAK2_RAT Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2) (Gamma-PAK) (p62-PAK)
Length = 524
Score = 72.8 bits (177), Expect = 4e-13
Identities = 34/99 (34%), Positives = 53/99 (53%)
Frame = +3
Query: 87 GTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFA 266
GT W APEV+ + YG DIWS G + +E++ G+ P +A+Y + T E
Sbjct: 405 GTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQ 464
Query: 267 YPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 383
P K S + F++ CL+ + R + K+L++H FL A
Sbjct: 465 NPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLA 503
>sp|Q8CIN4|PAK2_MOUSE Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2)
Length = 524
Score = 72.8 bits (177), Expect = 4e-13
Identities = 34/99 (34%), Positives = 53/99 (53%)
Frame = +3
Query: 87 GTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFA 266
GT W APEV+ + YG DIWS G + +E++ G+ P +A+Y + T E
Sbjct: 405 GTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQ 464
Query: 267 YPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 383
P K S + F++ CL+ + R + K+L++H FL A
Sbjct: 465 NPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLA 503
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,348,531
Number of Sequences: 369166
Number of extensions: 1170109
Number of successful extensions: 4573
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4316
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2871696580
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)