Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_L24
(890 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P78406|RAE1L_HUMAN mRNA-associated protein mrnp 41 (Rae1... 317 4e-86
sp|Q93454|NPP17_CAEEL Nucleoporin-17 (Nuclear pore complex ... 290 3e-78
sp|P41838|RAE1_SCHPO Poly(A)+ RNA export protein 205 1e-52
sp|P40066|GLE2_YEAST Nucleoporin GLE2 (Nuclear pore protein... 179 1e-44
sp|Q38942|RAE1L_ARATH Hypothetical rae1-like protein At1g80670 150 4e-36
sp|Q5RB58|BUB3_PONPY Mitotic checkpoint protein BUB3 137 4e-32
sp|O43684|BUB3_HUMAN Mitotic checkpoint protein BUB3 135 2e-31
sp|Q9WVA3|BUB3_MOUSE Mitotic checkpoint protein BUB3 (WD re... 134 3e-31
sp|P69104|GBLP_TRYBR Guanine nucleotide-binding protein bet... 55 2e-07
sp|O43017|SWD3_SCHPO Set1 complex component swd3 (Set1C com... 54 4e-07
>sp|P78406|RAE1L_HUMAN mRNA-associated protein mrnp 41 (Rae1 protein homolog)
Length = 368
Score = 317 bits (811), Expect = 4e-86
Identities = 149/284 (52%), Positives = 199/284 (70%), Gaps = 2/284 (0%)
Frame = +2
Query: 44 TSVMAFPNKPLFGTSNINVTQNTIKDIEIPNPPTDSVSCLRFSPPSLSQTYLMAGSWDKS 223
TS +FG++ + N +KDIE+ + P DS+ CL FSPP+L +L+AGSW
Sbjct: 7 TSGFGTSGTSMFGSATTD-NHNPMKDIEVTSSPDDSIGCLSFSPPTLPGNFLIAGSWAND 65
Query: 224 IRMWEISNTGEVIPRIQQNMQAPILDSCWSNDGTKTYISCVDKAGYMWDVAANQITKIAA 403
+R WE+ ++G+ IP+ QQ P+LD CWS+DG+K + + DK MWD+++NQ +IA
Sbjct: 66 VRCWEVQDSGQTIPKAQQMHTGPVLDVCWSDDGSKVFTASCDKTAKMWDLSSNQAIQIAQ 125
Query: 404 HDAPIKTIHYIQSSTYTCVMTGGWDKKLKFWDLRSQNPMSVIDLSDRIYCAAVHFPMAAV 583
HDAP+KTIH+I++ Y+CVMTG WDK LKFWD RS NPM V+ L +R YCA V +PMA V
Sbjct: 126 HDAPVKTIHWIKAPNYSCVMTGSWDKTLKFWDTRSSNPMMVLQLPERCYCADVIYPMAVV 185
Query: 584 ATGDKSVFVYNLENGPVEYKRLESQLKYQSRCISIFLDKSNSSPAGYALGSIEGRVGIQC 763
AT ++ + VY LEN P E++R+ES LK+Q RC++IF DK N P G+ALGSIEGRV I
Sbjct: 186 ATAERGLIVYQLENQPSEFRRIESPLKHQHRCVAIFKDKQN-KPTGFALGSIEGRVAIHY 244
Query: 764 LASQNP-KDSFTFKCHRSQVTNNNT-QDIFAVNDLAFHPSYQTL 889
+ NP KD+FTFKCHRS TN + QDI+AVN +AFHP + TL
Sbjct: 245 INPPNPAKDNFTFKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTL 288
>sp|Q93454|NPP17_CAEEL Nucleoporin-17 (Nuclear pore complex protein 17) (CeRAE1)
Length = 373
Score = 290 bits (743), Expect = 3e-78
Identities = 141/289 (48%), Positives = 188/289 (65%), Gaps = 12/289 (4%)
Frame = +2
Query: 59 FPNKPLFGTSNI---------NVTQNTIKDIEIPNPPTDSVSCLRFSPPSLSQTYLMAGS 211
F NK +FG SNI N TQN D + P D++ ++FSP + L GS
Sbjct: 7 FGNKSMFGGSNISTSTTTPAANTTQND--DFLVDGAPEDTIQVIKFSPTPQDKPMLACGS 64
Query: 212 WDKSIRMWEISNTGEVIPRIQQNMQAPILDSCWSNDGTKTYISCVDKAGYMWDVAANQIT 391
WD +IR+W ++ + QQN+ APILD W D +K +I+C DK +WD+A+NQ+
Sbjct: 65 WDGTIRVWMFNDANTFEGKAQQNIPAPILDIAWIEDSSKIFIACADKEARLWDLASNQVA 124
Query: 392 KIAAHDAPIKTIHYIQSSTYTCVMTGGWDKKLKFWDLRS---QNPMSVIDLSDRIYCAAV 562
+ HD P+KT H+I + Y C+MTG +DK L+FWD+++ Q M+ I L +R+Y A V
Sbjct: 125 VVGTHDGPVKTCHWINGNNYQCLMTGSFDKTLRFWDMKNLPNQTQMAQIQLPERVYAADV 184
Query: 563 HFPMAAVATGDKSVFVYNLENGPVEYKRLESQLKYQSRCISIFLDKSNSSPAGYALGSIE 742
+PMA VA +K + VYNLENGP E K +ESQLK+Q RCISIF DKSN +PAG+ALGSIE
Sbjct: 185 LYPMAVVALANKHIKVYNLENGPTEVKDIESQLKFQIRCISIFKDKSNQNPAGFALGSIE 244
Query: 743 GRVGIQCLASQNPKDSFTFKCHRSQVTNNNTQDIFAVNDLAFHPSYQTL 889
GRV +Q + NPKD+FTFKCHRS N Q+I+AVND+ FHP + TL
Sbjct: 245 GRVAVQYVDVANPKDNFTFKCHRSAELVNGFQEIYAVNDICFHPQHGTL 293
>sp|P41838|RAE1_SCHPO Poly(A)+ RNA export protein
Length = 352
Score = 205 bits (522), Expect = 1e-52
Identities = 105/276 (38%), Positives = 162/276 (58%), Gaps = 5/276 (1%)
Frame = +2
Query: 74 LFG---TSNI-NVTQNTIKDIEIPNPPTDSVSCLRFSPPSLSQTYLMAGSWDKSIRMWEI 241
LFG TS + N T + KD+E+ PP DS+S L FSP + YL A SWD +R++E+
Sbjct: 3 LFGQATTSTVSNATGDLKKDVEVAQPPEDSISDLAFSPQA---EYLAASSWDSKVRIYEV 59
Query: 242 SNTGEVIPRIQQNMQAPILDSCWSNDGTKTYISCVDKAGYMWDVAANQITKIAAHDAPIK 421
TG+ I + Q P+L WS DGTK VDK+ ++D+ Q ++AAHD ++
Sbjct: 60 QATGQSIGKALYEHQGPVLSVNWSRDGTKVASGSVDKSAKVFDIQTGQNQQVAAHDDAVR 119
Query: 422 TIHYIQS-STYTCVMTGGWDKKLKFWDLRSQNPMSVIDLSDRIYCAAVHFPMAAVATGDK 598
+ ++++ T + TG WDK LK+WDLR P++ + L +R+Y P+ VAT ++
Sbjct: 120 CVRFVEAMGTSPILATGSWDKTLKYWDLRQSTPIATVSLPERVYAMDCVHPLLTVATAER 179
Query: 599 SVFVYNLENGPVEYKRLESQLKYQSRCISIFLDKSNSSPAGYALGSIEGRVGIQCLASQN 778
++ V NL +K S LK+Q+R ++ F+ GYA+GS+EGR IQ + +N
Sbjct: 180 NICVINLSEPTKIFKLAMSPLKFQTRSLACFIKGD-----GYAIGSVEGRCAIQNIDEKN 234
Query: 779 PKDSFTFKCHRSQVTNNNTQDIFAVNDLAFHPSYQT 886
+F+F+CHR+Q N+ D+++VN +AFHP Y T
Sbjct: 235 ASQNFSFRCHRNQA--GNSADVYSVNSIAFHPQYGT 268
>sp|P40066|GLE2_YEAST Nucleoporin GLE2 (Nuclear pore protein GLE2) (poly(A) RNA export
protein RAE1)
Length = 365
Score = 179 bits (453), Expect = 1e-44
Identities = 99/279 (35%), Positives = 151/279 (54%), Gaps = 10/279 (3%)
Frame = +2
Query: 80 GTSNINVTQNTI-KDIEIPNPPTDSVSCLRFSPPSLSQTYLM-AGSWDKSIRMWEISNTG 253
GTS + + DI I +P DS+S + FSP Q ++ A SWD +R+W++ N G
Sbjct: 14 GTSTAMANEKDLANDIVINSPAEDSISDIAFSP---QQDFMFSASSWDGKVRIWDVQN-G 69
Query: 254 EVIPRIQQNMQAPILDSCWSNDGTKTYISCVDKAGYMWDVAANQITKIAAHDAPIKTIHY 433
R Q +P+L + WSNDGTK D A ++D+A+ Q +I H APIK + +
Sbjct: 70 VPQGRAQHESSSPVLCTRWSNDGTKVASGGCDNALKLYDIASGQTQQIGMHSAPIKVLRF 129
Query: 434 IQS--STYTCVMTGGWDKKLKFWDLRSQNPMSVIDLSDRIYCAAVHFPMAAVATGDKSVF 607
+Q S C++TG WDK +K+WD+R P+S + + +R+Y + VAT ++ +
Sbjct: 130 VQCGPSNTECIVTGSWDKTIKYWDMRQPQPVSTVMMPERVYSMDNKQSLLVVATAERHIA 189
Query: 608 VYNLENGPVEYKRLESQLKYQSRCISIFLDKSNSSPAGYALGSIEGRVGIQCL-ASQNPK 784
+ NL N +K S LK+Q+RC++ + + GYA+GS+EGR I+ + K
Sbjct: 190 IINLANPTTIFKATTSPLKWQTRCVACY-----NEADGYAIGSVEGRCSIRYIDDGMQKK 244
Query: 785 DSFTFKCHRSQVTN-----NNTQDIFAVNDLAFHPSYQT 886
F+FKCHR N N ++ VN +AFHP Y T
Sbjct: 245 SGFSFKCHRQTNPNRAPGSNGQSLVYPVNSIAFHPLYGT 283
>sp|Q38942|RAE1L_ARATH Hypothetical rae1-like protein At1g80670
Length = 251
Score = 150 bits (380), Expect = 4e-36
Identities = 77/202 (38%), Positives = 113/202 (55%), Gaps = 4/202 (1%)
Frame = +2
Query: 107 NTIKDIEIPNPPTDSVSCLRFSPPSLSQTYLMAGSWDKSIRMWEISNTGEVI---PRIQQ 277
N K E+ P DS+S L FSP + L+A SWD +R WEIS +G + P+
Sbjct: 13 NPNKSYEVTPSPADSISSLSFSPRA---DILVATSWDNQVRCWEISRSGASLASAPKASI 69
Query: 278 NMQAPILDSCWSNDGTKTYISCVDKAGYMWDV-AANQITKIAAHDAPIKTIHYIQSSTYT 454
+ P+L S W +DGT + DK MW + + Q +A H+ PI + +I
Sbjct: 70 SHDQPVLCSAWKDDGTTVFSGGCDKQAKMWPLLSGGQPVTVAMHEGPIAAMAWIPGMNL- 128
Query: 455 CVMTGGWDKKLKFWDLRSQNPMSVIDLSDRIYCAAVHFPMAAVATGDKSVFVYNLENGPV 634
+ TG WDK LK+WD R QNP+ L D+ Y +V P+ V T D+++ V+NL+N
Sbjct: 129 -LATGSWDKTLKYWDTRQQNPVHTQQLPDKCYTLSVKHPLMVVGTADRNLIVFNLQNPQT 187
Query: 635 EYKRLESQLKYQSRCISIFLDK 700
E+KR++S LKYQ+RC++ F D+
Sbjct: 188 EFKRIQSPLKYQTRCVTAFPDQ 209
>sp|Q5RB58|BUB3_PONPY Mitotic checkpoint protein BUB3
Length = 328
Score = 137 bits (345), Expect = 4e-32
Identities = 82/258 (31%), Positives = 131/258 (50%), Gaps = 2/258 (0%)
Frame = +2
Query: 119 DIEIPNPPTDSVSCLRFSPPSLSQTYLMAGSWDKSIRMWEISNTGEVIPRIQQNMQAPIL 298
+ ++ PP D +S ++FSP + +L+ SWD S+R++++ R++ +L
Sbjct: 6 EFKLNQPPEDGISSVKFSPNT--SQFLLVSSWDTSVRLYDVPANSM---RLKYQHTGAVL 60
Query: 299 DSCWSNDGTKTYISCVDKAGYMWDVAANQITKIAAHDAPIKTIHYIQSSTYTCVMTGGWD 478
D C D T + +D M D+ +Q + HDAPI+ + Y + TG WD
Sbjct: 61 D-CAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPE--VNVMATGSWD 117
Query: 479 KKLKFWDLRSQNPMSVIDLSDRIYCAAVHFPMAAVATGDKSVFVYNLENGPVEYKRLESQ 658
+ +K WD R+ +++Y +V V T + V V++L N +R ES
Sbjct: 118 QTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESS 177
Query: 659 LKYQSRCISIFLDKSNSSPAGYALGSIEGRVGIQCL--ASQNPKDSFTFKCHRSQVTNNN 832
LKYQ+RCI FL+K GY L SIEGRV ++ L + + K + FKCHR + NN
Sbjct: 178 LKYQTRCIRAFLNKQ-----GYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHR--LKENN 230
Query: 833 TQDIFAVNDLAFHPSYQT 886
+ I+ VN ++FH + T
Sbjct: 231 IEQIYPVNAISFHNIHNT 248
>sp|O43684|BUB3_HUMAN Mitotic checkpoint protein BUB3
Length = 328
Score = 135 bits (340), Expect = 2e-31
Identities = 81/258 (31%), Positives = 131/258 (50%), Gaps = 2/258 (0%)
Frame = +2
Query: 119 DIEIPNPPTDSVSCLRFSPPSLSQTYLMAGSWDKSIRMWEISNTGEVIPRIQQNMQAPIL 298
+ ++ PP D +S ++FSP + +L+ SWD S+R++++ R++ +L
Sbjct: 6 EFKLNQPPEDGISSVKFSPNT--SQFLLVSSWDTSVRLYDVPANSM---RLKYQHTGAVL 60
Query: 299 DSCWSNDGTKTYISCVDKAGYMWDVAANQITKIAAHDAPIKTIHYIQSSTYTCVMTGGWD 478
D C D T + +D M D+ +Q + HDAPI+ + Y ++TG WD
Sbjct: 61 D-CAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPE--VNVMVTGSWD 117
Query: 479 KKLKFWDLRSQNPMSVIDLSDRIYCAAVHFPMAAVATGDKSVFVYNLENGPVEYKRLESQ 658
+ +K WD R+ +++Y +V V T + V V++L N +R ES
Sbjct: 118 QTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESS 177
Query: 659 LKYQSRCISIFLDKSNSSPAGYALGSIEGRVGIQCL--ASQNPKDSFTFKCHRSQVTNNN 832
LKYQ+RCI F +K GY L SIEGRV ++ L + + K + FKCHR + NN
Sbjct: 178 LKYQTRCIRAFPNKQ-----GYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHR--LKENN 230
Query: 833 TQDIFAVNDLAFHPSYQT 886
+ I+ VN ++FH + T
Sbjct: 231 IEQIYPVNAISFHNIHNT 248
>sp|Q9WVA3|BUB3_MOUSE Mitotic checkpoint protein BUB3 (WD repeat type I transmembrane
protein A72.5)
Length = 326
Score = 134 bits (337), Expect = 3e-31
Identities = 81/258 (31%), Positives = 131/258 (50%), Gaps = 2/258 (0%)
Frame = +2
Query: 119 DIEIPNPPTDSVSCLRFSPPSLSQTYLMAGSWDKSIRMWEISNTGEVIPRIQQNMQAPIL 298
+ ++ PP D +S ++FSP + +L+ SWD S+R++++ R++ +L
Sbjct: 6 EFKLNQPPEDGISSVKFSPNT--SQFLLVSSWDTSVRLYDVPANSM---RLKYQHTGAVL 60
Query: 299 DSCWSNDGTKTYISCVDKAGYMWDVAANQITKIAAHDAPIKTIHYIQSSTYTCVMTGGWD 478
D C D T + +D M D+ +Q + HDAPI+ + Y ++TG WD
Sbjct: 61 D-CAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPE--VNVMVTGSWD 117
Query: 479 KKLKFWDLRSQNPMSVIDLSDRIYCAAVHFPMAAVATGDKSVFVYNLENGPVEYKRLESQ 658
+ +K WD R+ +++Y +V V T + V V++L N +R ES
Sbjct: 118 QTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLWNMGYVQQRRESS 177
Query: 659 LKYQSRCISIFLDKSNSSPAGYALGSIEGRVGIQCL--ASQNPKDSFTFKCHRSQVTNNN 832
LKYQ+RCI F +K GY L SIEGRV ++ L + + K + FKCHR + NN
Sbjct: 178 LKYQTRCIRAFPNKQ-----GYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHR--LKENN 230
Query: 833 TQDIFAVNDLAFHPSYQT 886
+ I+ VN ++FH + T
Sbjct: 231 IEQIYPVNAISFHNIHNT 248
>sp|P69104|GBLP_TRYBR Guanine nucleotide-binding protein beta subunit-like protein
(Activated protein kinase C receptor homolog) (Track)
sp|P69103|GBLP_TRYBB Guanine nucleotide-binding protein beta subunit-like protein
(Activated protein kinase C receptor homolog)
Length = 318
Score = 55.5 bits (132), Expect = 2e-07
Identities = 34/124 (27%), Positives = 64/124 (51%)
Frame = +2
Query: 143 TDSVSCLRFSPPSLSQTYLMAGSWDKSIRMWEISNTGEVIPRIQQNMQAPILDSCWSNDG 322
TD VSC+RFSP SL +++G WD +++W+++ TG ++ ++ + + S DG
Sbjct: 152 TDWVSCVRFSP-SLDAPVIVSGGWDNLVKVWDLA-TGRLVTDLKGHTNY-VTSVTVSPDG 208
Query: 323 TKTYISCVDKAGYMWDVAANQITKIAAHDAPIKTIHYIQSSTYTCVMTGGWDKKLKFWDL 502
+ S D +WD+ + A API I + + + C T +K ++ +DL
Sbjct: 209 SLCASSDKDGVARLWDLTKGEALSEMAAGAPINQICFSPNRYWMCAAT---EKGIRIFDL 265
Query: 503 RSQN 514
+++
Sbjct: 266 ENKD 269
Score = 46.2 bits (108), Expect = 1e-04
Identities = 37/167 (22%), Positives = 69/167 (41%), Gaps = 17/167 (10%)
Frame = +2
Query: 53 MAFPNKPLFGTSNINVTQNTIKDIEIPNPPTDSVSCLRFSPP---------------SLS 187
+A P P T ++ +++ PNP S C P S +
Sbjct: 19 LACPQTPETATKVVSTSRDKTLLSWGPNPDRHSSECSYGLPDRRLEGHSAFVSDVALSNN 78
Query: 188 QTYLMAGSWDKSIRMWEISNTGEVIPRIQQNMQAPILDSCWSNDGTKTYISCVDKAGYMW 367
+ ++ SWD S+R+W + N G+ + + + +L +S D + D A +W
Sbjct: 79 GNFAVSASWDHSLRLWNLQN-GQCQYKFLGHTK-DVLSVAFSPDNRQIVSGGRDNALRVW 136
Query: 368 DVAANQITKIA--AHDAPIKTIHYIQSSTYTCVMTGGWDKKLKFWDL 502
+V + ++ AH + + + S +++GGWD +K WDL
Sbjct: 137 NVKGECMHTLSRGAHTDWVSCVRFSPSLDAPVIVSGGWDNLVKVWDL 183
Score = 31.2 bits (69), Expect = 4.1
Identities = 20/77 (25%), Positives = 35/77 (45%), Gaps = 5/77 (6%)
Frame = +2
Query: 167 FSPPSLSQTYLMAGSWDKSIRMWEISNTGEVIPRIQQNMQAP-ILDSC----WSNDGTKT 331
FSP Y M + +K IR++++ N ++ ++ + I+ C WS DG+
Sbjct: 245 FSP----NRYWMCAATEKGIRIFDLENKDIIVELAPEHQGSKKIVPECVSIAWSADGSTL 300
Query: 332 YISCVDKAGYMWDVAAN 382
Y D +W V+ N
Sbjct: 301 YSGYTDNVIRVWGVSEN 317
>sp|O43017|SWD3_SCHPO Set1 complex component swd3 (Set1C component swd3) (COMPASS
component swd3) (Complex proteins associated with set1
protein swd3)
Length = 380
Score = 54.3 bits (129), Expect = 4e-07
Identities = 32/132 (24%), Positives = 66/132 (50%), Gaps = 2/132 (1%)
Frame = +2
Query: 143 TDSVSCLRFSPPSLSQTYLMAGSWDKSIRMWEISNTGEVIPRIQQNMQAPILDSCWSNDG 322
T+ VS + F+P T L++GSWD+++R+W + + G + + + + PI+ S DG
Sbjct: 138 TNYVSSIDFNPLG---TLLVSGSWDETVRIWNLQD-GTCLRMLPAHSE-PIISVSISADG 192
Query: 323 TKTYISCVDKAGYMWDVAANQITK--IAAHDAPIKTIHYIQSSTYTCVMTGGWDKKLKFW 496
T + D +WDV + Q K + + P+ + + ++ Y ++ + +++ W
Sbjct: 193 TLCATASYDGMARIWDVLSGQCLKTLVEPINVPLSNLQFTENRKY--LLVSNLNSQIRLW 250
Query: 497 DLRSQNPMSVID 532
D R + + D
Sbjct: 251 DYRRNRVVRIFD 262
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,073,585
Number of Sequences: 369166
Number of extensions: 2050995
Number of successful extensions: 6027
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5742
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8934348180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)