Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_L18
(432 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8CST4|IF2_STAES Translation initiation factor IF-2 >gi|... 42 6e-04
sp|P38691|KSP1_YEAST Serine/threonine-protein kinase KSP1 42 7e-04
sp|Q17060|MRJP3_APIME Major royal jelly protein 3 precursor... 40 0.003
sp|Q9Y2W1|TR150_HUMAN Thyroid hormone receptor-associated p... 40 0.003
sp|Q8WXA9|SFR12_HUMAN Splicing factor, arginine/serine-rich... 39 0.004
sp|P25339|PUF4_YEAST PUF4 protein 39 0.005
sp|Q8BZX4|SFR12_MOUSE Splicing factor, arginine/serine-rich... 37 0.014
sp|Q5UP41|YR871_MIMIV Hypothetical protein R871 37 0.014
sp|Q9NRR4|RNC_HUMAN Ribonuclease III (RNase III) (Drosha) (... 37 0.014
sp|Q01443|SSP2_PLAYO Sporozoite surface protein 2 precursor 37 0.018
>sp|Q8CST4|IF2_STAES Translation initiation factor IF-2
sp|Q5HPS2|IF2_STAEQ Translation initiation factor IF-2
Length = 720
Score = 42.0 bits (97), Expect = 6e-04
Identities = 28/98 (28%), Positives = 49/98 (50%)
Frame = +2
Query: 137 KKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 316
K + K++ S+ K NK++ +NH Q SN+ +++++K K+N + KK K
Sbjct: 44 KALDKKFKASQAKDTNKQNTQNNH--QKSNNKQNSNDKEKQQSKNNS--KPTKKKEQNNK 99
Query: 317 RKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 430
K +NN +Q +NK K+ +KN E E+
Sbjct: 100 GKQQNKNNKTNKNQKNNKN----KKNNKNNKPQNEVEE 133
>sp|P38691|KSP1_YEAST Serine/threonine-protein kinase KSP1
Length = 1029
Score = 41.6 bits (96), Expect = 7e-04
Identities = 23/87 (26%), Positives = 42/87 (48%), Gaps = 3/87 (3%)
Frame = +2
Query: 110 RDPYKRRE---PKKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNED 280
RD + R+ P +++Y +NNK+ R + +Y +N+ N NH N+ +
Sbjct: 516 RDEFNTRDFFTPPSVQHRYMEGFSNNNNKQYRQNRNYNNNNNNSNNNHGSNYNNFNNGNS 575
Query: 281 YERPSKKNSYEKRKPVKQNNYYGDSQM 361
Y + KN + R+P ++Y G S +
Sbjct: 576 YIKGWNKNFNKYRRP-SSSSYTGKSPL 601
Score = 30.8 bits (68), Expect = 1.3
Identities = 21/68 (30%), Positives = 36/68 (52%), Gaps = 2/68 (2%)
Frame = +2
Query: 143 HENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRK--KNDMKDNEDYERPSKKNSYEK 316
+ +YR +R NN + S+N++ SN+ N+N+ K K+ Y RPS +SY
Sbjct: 542 NNKQYRQNR-NYNNNNNNSNNNH--GSNYNNFNNGNSYIKGWNKNFNKYRRPS-SSSYTG 597
Query: 317 RKPVKQNN 340
+ P+ + N
Sbjct: 598 KSPLSRYN 605
>sp|Q17060|MRJP3_APIME Major royal jelly protein 3 precursor (MRJP-3) (Bee-milk protein)
(Royal jelly protein RJP57-1)
Length = 544
Score = 39.7 bits (91), Expect = 0.003
Identities = 22/85 (25%), Positives = 43/85 (50%), Gaps = 2/85 (2%)
Frame = +2
Query: 122 KRREPKKHENKYRTSRYKSNNKRDRSSNHYYQPSN--HGNYNHNRKKNDMKDNEDYERPS 295
K+ ++++N+ ++ N + D N Q N +GN + K+ND K N + + +
Sbjct: 454 KQNGNRQNDNRQNDNKQNGNRQNDNKQNGNRQNDNKQNGNRQNGNKQNDNKQNGNRQNDN 513
Query: 296 KKNSYEKRKPVKQNNYYGDSQMDNK 370
K+N R+ QNN +++ DN+
Sbjct: 514 KRNG--NRQNDNQNNQNDNNRNDNQ 536
Score = 34.7 bits (78), Expect = 0.088
Identities = 22/88 (25%), Positives = 42/88 (47%)
Frame = +2
Query: 140 KHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKR 319
++ NK +R N + D N GN ++ K+N + N++ + +++N K+
Sbjct: 450 QNANKQNGNRQNDNRQNDNKQN--------GNRQNDNKQNGNRQNDNKQNGNRQNG-NKQ 500
Query: 320 KPVKQNNYYGDSQMDNKKPAEIKRQSKN 403
KQN G+ Q DNK+ + ++N
Sbjct: 501 NDNKQN---GNRQNDNKRNGNRQNDNQN 525
Score = 32.0 bits (71), Expect = 0.57
Identities = 18/77 (23%), Positives = 40/77 (51%), Gaps = 2/77 (2%)
Frame = +2
Query: 176 SNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKRKPVKQ--NNYYG 349
+ N ++++++ +GN ++ ++ND K N + + +K+N ++ KQ N G
Sbjct: 439 NQNANNQNADNQNANKQNGNRQNDNRQNDNKQNGNRQNDNKQNG-NRQNDNKQNGNRQNG 497
Query: 350 DSQMDNKKPAEIKRQSK 400
+ Q DNK+ + +K
Sbjct: 498 NKQNDNKQNGNRQNDNK 514
>sp|Q9Y2W1|TR150_HUMAN Thyroid hormone receptor-associated protein complex 150 kDa
component (Trap150) (Thyroid hormone receptor associated
protein 3)
Length = 955
Score = 39.7 bits (91), Expect = 0.003
Identities = 25/100 (25%), Positives = 41/100 (41%), Gaps = 2/100 (2%)
Frame = +2
Query: 122 KRREPKKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKN--DMKDNEDYERPS 295
KRR P + + KS++ R R S+ SNH +++K+ + K + RPS
Sbjct: 131 KRRSPSPRSRSHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSAKEKKSSSKDSRPS 190
Query: 296 KKNSYEKRKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKT 415
+ + VK+ + G + D K K Y T
Sbjct: 191 QAAGDNQGDEVKEQTFSGGTSQDTKASESSKPWPDATYGT 230
>sp|Q8WXA9|SFR12_HUMAN Splicing factor, arginine/serine-rich 12 (Serine-arginine-rich
splicing regulatory protein 86) (SRrp86) (Splicing
regulatory protein 508) (SRrp508)
Length = 508
Score = 39.3 bits (90), Expect = 0.004
Identities = 25/99 (25%), Positives = 46/99 (46%), Gaps = 1/99 (1%)
Frame = +2
Query: 137 KKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 316
+ H S KS+++R RS S H + +HNR ++ KD + P KK S +
Sbjct: 193 RSHTRSKSRSSSKSHSRRKRSQ------SKHRSRSHNRSRSRQKDRRRSKSPHKKRSKSR 246
Query: 317 -RKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 430
R+ + ++ D + D ++ + K + K + E E+
Sbjct: 247 ERRKSRSRSHSRDKRKDTREKIKEKERVKEKDREKERER 285
Score = 31.2 bits (69), Expect = 0.97
Identities = 16/98 (16%), Positives = 46/98 (46%)
Frame = +2
Query: 137 KKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 316
K+ ++K+R+ + + R + P + + R+K+ + + +R + ++
Sbjct: 211 KRSQSKHRSRSHNRSRSRQKDRRRSKSPHKKRSKSRERRKSRSRSHSRDKRKDTREKIKE 270
Query: 317 RKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 430
++ VK+ + + + + ++ E K + KN + E E+
Sbjct: 271 KERVKEKDREKEREREKEREKE-KERGKNKDRDKEREK 307
>sp|P25339|PUF4_YEAST PUF4 protein
Length = 888
Score = 38.9 bits (89), Expect = 0.005
Identities = 20/78 (25%), Positives = 37/78 (47%), Gaps = 4/78 (5%)
Frame = +2
Query: 143 HENKYRTSRYKSNNKRDRSSN----HYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSY 310
H + NNK SN + S HG YN+N N+ +N + + NS
Sbjct: 485 HNDNDNIGNSNYNNKDTGRSNVGKMKNMKNSYHGYYNNNNNNNNNNNNNNNSNATNSNSA 544
Query: 311 EKRKPVKQNNYYGDSQMD 364
EK++ +++++ + D+ +D
Sbjct: 545 EKQRKIEESSRFADAVLD 562
>sp|Q8BZX4|SFR12_MOUSE Splicing factor, arginine/serine-rich 12 (Serine-arginine-rich
splicing regulatory protein 86) (SRrp86)
Length = 494
Score = 37.4 bits (85), Expect = 0.014
Identities = 25/99 (25%), Positives = 45/99 (45%), Gaps = 1/99 (1%)
Frame = +2
Query: 137 KKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 316
+ H S KS+++R RS S H + +HNR ++ KD + P KK S +
Sbjct: 196 RSHTRSKSRSSSKSHSRRKRSQ------SKHRSRSHNRSRSRQKDRRRSKSPHKKRSKSR 249
Query: 317 -RKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 430
R+ + + D + D ++ + + + K K E E+
Sbjct: 250 ERRKSRSRSRSRDKRKDTREKVKERVKEKEREKEREREK 288
Score = 30.0 bits (66), Expect = 2.2
Identities = 18/98 (18%), Positives = 41/98 (41%)
Frame = +2
Query: 137 KKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 316
K+ ++K+R+ + + R + P + + R+K+ + +R + ++
Sbjct: 214 KRSQSKHRSRSHNRSRSRQKDRRRSKSPHKKRSKSRERRKSRSRSRSRDKRKDTREKVKE 273
Query: 317 RKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 430
R K+ + + D +K K + KN K E E+
Sbjct: 274 RVKEKEREKEREREKDREKD---KERGKNKDKDREKEK 308
>sp|Q5UP41|YR871_MIMIV Hypothetical protein R871
Length = 561
Score = 37.4 bits (85), Expect = 0.014
Identities = 20/81 (24%), Positives = 39/81 (48%), Gaps = 2/81 (2%)
Frame = +2
Query: 110 RDPYKRREPKKHEN--KYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDY 283
+ P + R PK+H++ K+++S+ ++K D+SS H ++ KK D + Y
Sbjct: 129 KSPSRGRSPKRHKSSKKHKSSKKHKSSKHDKSSKRDKSSKRHKSH----KKKDKSHKKRY 184
Query: 284 ERPSKKNSYEKRKPVKQNNYY 346
PS S + + ++ Y
Sbjct: 185 RSPSSDRSLSRDRSSSRDRSY 205
>sp|Q9NRR4|RNC_HUMAN Ribonuclease III (RNase III) (Drosha) (p241)
Length = 1374
Score = 37.4 bits (85), Expect = 0.014
Identities = 24/102 (23%), Positives = 49/102 (48%), Gaps = 3/102 (2%)
Frame = +2
Query: 116 PYKRREPKKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYER-- 289
P +RR P++ ++ Y R++ ++ R H + +R++ D + DY+R
Sbjct: 216 PSERRSPERLKH-YDDHRHRDHS-HGRGERHRSLDRRERGRSPDRRRQDSRYRSDYDRGR 273
Query: 290 -PSKKNSYEKRKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYK 412
PS+ SYE+ + ++ + DN++ ++R K YK
Sbjct: 274 TPSRHRSYERSRERERERH---RHRDNRRSPSLERSYKKEYK 312
>sp|Q01443|SSP2_PLAYO Sporozoite surface protein 2 precursor
Length = 827
Score = 37.0 bits (84), Expect = 0.018
Identities = 24/107 (22%), Positives = 40/107 (37%)
Frame = +2
Query: 110 RDPYKRREPKKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYER 289
R+P +R K NK ++ N + + + +PSN N N N K N +
Sbjct: 395 RNPKRRNPNKPKPNKPNPNKPNPNEPSNPNKPNPNEPSNPNKPNPNEPSNPNKPNPN--E 452
Query: 290 PSKKNSYEKRKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 430
PS N +P+ N ++ N + NP + P +
Sbjct: 453 PSNPNKPNPNEPLNPNEPSNPNEPSNPNAPSNPNEPSNPNEPSNPNE 499
Score = 33.5 bits (75), Expect = 0.20
Identities = 22/69 (31%), Positives = 28/69 (40%), Gaps = 1/69 (1%)
Frame = +2
Query: 134 PKKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDN-EDYERPSKKNSY 310
PKK K R NK + + + +PSN N N N K N + P+K N
Sbjct: 392 PKKRNPKRRNPNKPKPNKPNPNKPNPNEPSNPNKPNPNEPSNPNKPNPNEPSNPNKPNPN 451
Query: 311 EKRKPVKQN 337
E P K N
Sbjct: 452 EPSNPNKPN 460
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,335,883
Number of Sequences: 369166
Number of extensions: 734535
Number of successful extensions: 4449
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3373
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3989
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2144903640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)