Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_K06
(273 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--pr... 75 5e-14
sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--pr... 74 8e-14
sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--pr... 73 2e-13
sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--pr... 72 3e-13
sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosacch... 65 4e-11
sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--pr... 55 4e-08
sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--pro... 42 4e-04
sp|P34374|UNC36_CAEEL Voltage-dependent calcium channel unc... 31 1.0
sp|O78432|YCF30_GUITH Putative HTH-type transcriptional reg... 30 2.3
sp|O26270|PURQ_METTH Phosphoribosylformylglycinamidine synt... 29 3.9
>sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit)
Length = 445
Score = 75.1 bits (183), Expect = 5e-14
Identities = 39/74 (52%), Positives = 50/74 (67%)
Frame = +2
Query: 41 GVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRID 220
GVLRVG VSH ++ P YT+ + V Y IVI++L ++G W PF DD+QLEF RID
Sbjct: 293 GVLRVGPVSHHRVGETAP-PNAYTVTDLVEYSIVIEQL-SNGRWVPFDGDDIQLEFVRID 350
Query: 221 PFVRQNMLKKEGKF 262
PFVR + KK GK+
Sbjct: 351 PFVRTFLKKKGGKY 364
>sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 439
Score = 74.3 bits (181), Expect = 8e-14
Identities = 39/74 (52%), Positives = 49/74 (66%)
Frame = +2
Query: 41 GVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRID 220
GVLRVG VSH ++ P YT+ + V Y IVI +L ++G W PF DD+QLEF RID
Sbjct: 287 GVLRVGPVSHHRVGETAP-PNAYTVTDLVEYSIVIQQL-SNGKWVPFDGDDIQLEFVRID 344
Query: 221 PFVRQNMLKKEGKF 262
PFVR + KK GK+
Sbjct: 345 PFVRTFLKKKGGKY 358
>sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 441
Score = 73.2 bits (178), Expect = 2e-13
Identities = 38/74 (51%), Positives = 49/74 (66%)
Frame = +2
Query: 41 GVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRID 220
GVLRVG VSH + P YT+ + V Y IVI++L ++G W PF DD+QLEF RID
Sbjct: 289 GVLRVGPVSHHRVGEMAP-PNAYTVTDLVEYSIVIEQL-SNGKWVPFDGDDIQLEFVRID 346
Query: 221 PFVRQNMLKKEGKF 262
PFVR + +K GK+
Sbjct: 347 PFVRTFLKRKGGKY 360
>sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit (Oligosaccharyl transferase 48 kDa subunit)
(OST 50 kDa subunit) (DDOST 48 kDa subunit)
Length = 413
Score = 72.4 bits (176), Expect = 3e-13
Identities = 38/74 (51%), Positives = 49/74 (66%)
Frame = +2
Query: 41 GVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRID 220
GVLRVG VSH + + P YT+ + V Y IVI++L +DG W PF DD+QLEF RID
Sbjct: 261 GVLRVGAVSHHRVGE-LAPPNAYTVTDLVEYSIVIEKL-SDGKWIPFDGDDIQLEFVRID 318
Query: 221 PFVRQNMLKKEGKF 262
PFVR + + GK+
Sbjct: 319 PFVRTFLKRNGGKY 332
>sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit precursor
(Oligosaccharyl transferase 48 kDa subunit)
Length = 445
Score = 65.5 bits (158), Expect = 4e-11
Identities = 36/80 (45%), Positives = 48/80 (60%)
Frame = +2
Query: 20 RMSVQGAGVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQ 199
R ++ +GVLRV V+H ++V Y I E+V Y I I+EL+ +G W PF DVQ
Sbjct: 274 RWVMKESGVLRVKTVNHHKKGETVPPVEGYFITEDVVYTIEIEELK-NGKWVPFQGKDVQ 332
Query: 200 LEFYRIDPFVRQNMLKKEGK 259
LEF RIDPFVR + G+
Sbjct: 333 LEFVRIDPFVRATLKNSNGR 352
>sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit) (OST5OP) (DrOST)
(DmOST50)
Length = 449
Score = 55.5 bits (132), Expect = 4e-08
Identities = 31/64 (48%), Positives = 38/64 (59%)
Frame = +2
Query: 41 GVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRID 220
G LRV +V H + + + YTI + V Y I I+EL G W F A D+QLEF RID
Sbjct: 286 GRLRVASVQHHKEGELLPPDQAYTITDPVVYTIGIEEL-VQGEWRAFKASDIQLEFVRID 344
Query: 221 PFVR 232
PFVR
Sbjct: 345 PFVR 348
>sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase beta
subunit precursor (Oligosaccharyl transferase beta
subunit)
Length = 430
Score = 42.0 bits (97), Expect = 4e-04
Identities = 21/63 (33%), Positives = 33/63 (52%)
Frame = +2
Query: 44 VLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRIDP 223
V++ + HS AD + + Y I + V Y + E + W P IADD+Q E ++DP
Sbjct: 263 VIKSVHAVHSHADGTSYDEEPYKIKDKVIYSVGFSEWNGE-EWLPHIADDIQFELRQVDP 321
Query: 224 FVR 232
+ R
Sbjct: 322 YYR 324
>sp|P34374|UNC36_CAEEL Voltage-dependent calcium channel unc-36 precursor (Uncoordinated
protein 36)
Length = 1249
Score = 30.8 bits (68), Expect = 1.0
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
Frame = -3
Query: 196 DIISNERSPCSISPQLVNNDLVMH--ILHYCVF 104
D NER PCS SP +V+ ++ LH+C+F
Sbjct: 1217 DDSENERRPCSTSPTIVSIFQILFGVFLHFCIF 1249
>sp|O78432|YCF30_GUITH Putative HTH-type transcriptional regulator ycf30
Length = 310
Score = 29.6 bits (65), Expect = 2.3
Identities = 14/43 (32%), Positives = 26/43 (60%)
Frame = +2
Query: 140 VIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLKKEGKFYL 268
V DELR+ TP++ D++ L ++ PF + +++KE + L
Sbjct: 155 VPDELRSKLQITPYVDDELALILPKLHPFSKLEVIQKEDLYRL 197
>sp|O26270|PURQ_METTH Phosphoribosylformylglycinamidine synthase I (FGAM synthase I)
Length = 218
Score = 28.9 bits (63), Expect = 3.9
Identities = 21/76 (27%), Positives = 35/76 (46%)
Frame = +2
Query: 44 VLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRIDP 223
VL + N + LA+ + P +T+ E+ + ELR T TPF ++ D
Sbjct: 81 VLGICNGAQILAEVGLV-PGVFTVNEHPKFNCQWTELRVKTTRTPFTG------LFKKDE 133
Query: 224 FVRQNMLKKEGKFYLD 271
+R + EG++Y D
Sbjct: 134 VIRMPVAHAEGRYYHD 149
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,082,491
Number of Sequences: 369166
Number of extensions: 649929
Number of successful extensions: 1704
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1662
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1694
length of database: 68,354,980
effective HSP length: 60
effective length of database: 57,270,880
effective search space used: 1718126400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)