Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_J08
(657 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P56399|UBP5_MOUSE Ubiquitin carboxyl-terminal hydrolase ... 154 2e-37
sp|P45974|UBP5_HUMAN Ubiquitin carboxyl-terminal hydrolase ... 153 3e-37
sp|Q92995|UBP13_HUMAN Ubiquitin carboxyl-terminal hydrolase... 147 2e-35
sp|P54201|UBPA_DICDI Ubiquitin carboxyl-terminal hydrolase ... 145 7e-35
sp|Q8L6Y1|UBP14_ARATH Ubiquitin carboxyl-terminal hydrolase... 144 3e-34
sp|P38237|UBP14_YEAST Ubiquitin carboxyl-terminal hydrolase... 73 7e-13
sp|Q11119|UBP14_SCHPO Ubiquitin carboxyl-terminal hydrolase... 67 4e-11
sp|Q80U87|UBP8_MOUSE Ubiquitin carboxyl-terminal hydrolase ... 39 0.012
sp|P40818|UBP8_HUMAN Ubiquitin carboxyl-terminal hydrolase ... 37 0.045
sp|P35123|UBP4_MOUSE Ubiquitin carboxyl-terminal hydrolase ... 35 0.22
>sp|P56399|UBP5_MOUSE Ubiquitin carboxyl-terminal hydrolase 5 (Ubiquitin thiolesterase 5)
(Ubiquitin-specific processing protease 5)
(Deubiquitinating enzyme 5) (Isopeptidase T)
Length = 858
Score = 154 bits (389), Expect = 2e-37
Identities = 77/215 (35%), Positives = 129/215 (60%), Gaps = 6/215 (2%)
Frame = +1
Query: 16 GITPQMFRMVIGQNHADFSTKQQQDAYEFLIHIMSMIQKDVVKHKNEKIRNPVESTALRI 195
GI P+MF+ +IG+ H +FST +QQDA EF +H+++M++++ NP E +
Sbjct: 411 GIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNC-----RSSENPNEVFRFLV 465
Query: 196 EDRIFCNTSQTVKYQYRDELVLSLPISLDDAINKDEVRASEA----ANADNIHSDDVIKP 363
E++I C ++ VKY R + ++ LP+ +D A+NK+E+ E A + + ++++
Sbjct: 466 EEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKVPLPELVRA 525
Query: 364 IVTFESCLNKFIQDDVILDYVVNG--QASIAYHSSKLETFPDYLWIQLKRFTVGDDWTPK 537
V F SCL + + + D+ S+A +++ +FPDYL IQ+K+FT G DW PK
Sbjct: 526 QVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPK 585
Query: 538 KLDVEVLVPDQLDLSHMKAKGLQPNEVEMKDEKEP 642
KLDV + +P++LD+S ++ GLQP E E+ D P
Sbjct: 586 KLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPP 620
>sp|P45974|UBP5_HUMAN Ubiquitin carboxyl-terminal hydrolase 5 (Ubiquitin thiolesterase 5)
(Ubiquitin-specific processing protease 5)
(Deubiquitinating enzyme 5) (Isopeptidase T)
Length = 858
Score = 153 bits (387), Expect = 3e-37
Identities = 77/215 (35%), Positives = 129/215 (60%), Gaps = 6/215 (2%)
Frame = +1
Query: 16 GITPQMFRMVIGQNHADFSTKQQQDAYEFLIHIMSMIQKDVVKHKNEKIRNPVESTALRI 195
GI P+MF+ +IG+ H +FST +QQDA EF +H+++M++++ NP E +
Sbjct: 411 GIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNC-----RSSENPNEVFRFLV 465
Query: 196 EDRIFCNTSQTVKYQYRDELVLSLPISLDDAINKDEVRASEA----ANADNIHSDDVIKP 363
E++I C ++ VKY R + ++ LP+ +D A+NK+E+ E A + + ++++
Sbjct: 466 EEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKMALPELVRA 525
Query: 364 IVTFESCLNKFIQDDVILDYVVNG--QASIAYHSSKLETFPDYLWIQLKRFTVGDDWTPK 537
V F SCL + + + D+ S+A +++ +FPDYL IQ+K+FT G DW PK
Sbjct: 526 QVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPK 585
Query: 538 KLDVEVLVPDQLDLSHMKAKGLQPNEVEMKDEKEP 642
KLDV + +P++LD+S ++ GLQP E E+ D P
Sbjct: 586 KLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPP 620
>sp|Q92995|UBP13_HUMAN Ubiquitin carboxyl-terminal hydrolase 13 (Ubiquitin thiolesterase 13)
(Ubiquitin-specific processing protease 13)
(Deubiquitinating enzyme 13) (Isopeptidase T-3) (ISOT-3)
Length = 863
Score = 147 bits (372), Expect = 2e-35
Identities = 72/215 (33%), Positives = 133/215 (61%), Gaps = 6/215 (2%)
Frame = +1
Query: 16 GITPQMFRMVIGQNHADFSTKQQQDAYEFLIHIMSMIQKDVVKHKNEKIRNPVESTALRI 195
GI+P+MF+ + ++H +FS+ +QQDA EF +H++++++++ + +N P + +
Sbjct: 423 GISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNRIGSEN-----PSDVFRFLV 477
Query: 196 EDRIFCNTSQTVKYQYRDELVLSLPISLDDAINKDEVRASEA----ANADNIHSDDVIKP 363
E+RI C ++ V+Y R + ++ LP++++ A NKDE+ A E A A+ ++++
Sbjct: 478 EERIQCCQTRKVRYTERVDYLMQLPVAMEAATNKDELIAYELTRREAEANRRPLPELVRA 537
Query: 364 IVTFESCLNKFIQDDVILDYVVNG--QASIAYHSSKLETFPDYLWIQLKRFTVGDDWTPK 537
+ F +CL F + + + D+ + S +S+ +FP+YL +Q+K+FT G DW PK
Sbjct: 538 KIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPK 597
Query: 538 KLDVEVLVPDQLDLSHMKAKGLQPNEVEMKDEKEP 642
K DV + +PD LD++H++A+GLQP E E+ D P
Sbjct: 598 KFDVSIDMPDLLDINHLRARGLQPGEEELPDISPP 632
>sp|P54201|UBPA_DICDI Ubiquitin carboxyl-terminal hydrolase A (Ubiquitin thiolesterase A)
(Ubiquitin-specific processing protease A)
(Deubiquitinating enzyme A)
Length = 837
Score = 145 bits (367), Expect = 7e-35
Identities = 80/214 (37%), Positives = 129/214 (60%), Gaps = 9/214 (4%)
Frame = +1
Query: 16 GITPQMFRMVIGQNHADFSTKQQQDAYEFLIHIMSMIQKDVVKHKNE---KIRNPVESTA 186
GI P+MF+ +IG +HA+FST +QQDA+E+L +++ I++ +H + NP
Sbjct: 404 GIAPKMFKSLIGASHAEFSTMKQQDAHEYLQYLLEYIER--AEHSRPSWIQQANPTRLFQ 461
Query: 187 LRIEDRIFCNTSQTVKYQYRDELVLSLPISLDDAINKDEVRASEAA----NADNIHSDDV 354
EDRI C +S VKY R E +LS+P++LDDA NK EV E N +
Sbjct: 462 FHNEDRIECGSSGQVKYTRRLENILSVPVNLDDATNKQEVAQYEETLKQQNGVRQKDQEE 521
Query: 355 IKPIVTFESCLNKFIQDDVILDYV--VNGQASIAYHSSKLETFPDYLWIQLKRFTVGDDW 528
I+PI+ SC+N F++ + D++ G + + +SS++ TFP+ L I LK++T D+
Sbjct: 522 IRPIIPLVSCINGFVEPYRVEDFLSPATGVKTFSLNSSRMATFPEVLIIHLKKYTYNADY 581
Query: 529 TPKKLDVEVLVPDQLDLSHMKAKGLQPNEVEMKD 630
TPKKL+V + VPD +D+ ++ +G++ EV +K+
Sbjct: 582 TPKKLNVFMDVPDIIDIDSLRGRGIKEGEVPLKE 615
>sp|Q8L6Y1|UBP14_ARATH Ubiquitin carboxyl-terminal hydrolase 14 (Ubiquitin thiolesterase 14)
(Ubiquitin-specific processing protease 14)
(Deubiquitinating enzyme 14) (AtUBP14) (TITAN-6 protein)
Length = 797
Score = 144 bits (362), Expect = 3e-34
Identities = 79/214 (36%), Positives = 125/214 (58%), Gaps = 9/214 (4%)
Frame = +1
Query: 16 GITPQMFRMVIGQNHADFSTKQQQDAYEFLIHIMSMIQKDVVKHKNEKIRNPVESTALRI 195
GI P+MF+ VI +HA+FS+ +QQDA +F +H++ +++ +P S I
Sbjct: 391 GIPPRMFKNVIAASHAEFSSMRQQDALDFFLHLVGKVER---ASNTTPDLDPSRSFKFGI 447
Query: 196 EDRIFCNTSQTVKYQYRDELVLSLPISLDDAINKDEVRASEAANA-------DNIHSDDV 354
E++I C + + V Y R++ +LSL I L +A NKDE+ A A D SD++
Sbjct: 448 EEKILCPSGK-VGYNKREDCILSLNIPLHEATNKDELEAFHKQKAGKGLEENDMRSSDEI 506
Query: 355 IKPIVTFESCLNKFIQDDVILDYVVNGQASI--AYHSSKLETFPDYLWIQLKRFTVGDDW 528
++P V E+CL F + I DY + + A ++ L +FPDYL + +++F + + W
Sbjct: 507 VRPRVPLEACLANFASSEPIEDYYSSALKGMTTAIKTTGLTSFPDYLVLHMRKFVMEEGW 566
Query: 529 TPKKLDVEVLVPDQLDLSHMKAKGLQPNEVEMKD 630
PKKLDV + VPD +D+SHM++KGLQP E + D
Sbjct: 567 VPKKLDVYIDVPDVIDISHMRSKGLQPGEELLPD 600
>sp|P38237|UBP14_YEAST Ubiquitin carboxyl-terminal hydrolase 14 (Ubiquitin thiolesterase 14)
(Ubiquitin-specific processing protease 14)
(Deubiquitinating enzyme 14) (Glucose-induced degradation
protein 6)
Length = 803
Score = 72.8 bits (177), Expect = 7e-13
Identities = 59/224 (26%), Positives = 94/224 (41%), Gaps = 8/224 (3%)
Frame = +1
Query: 7 PNPGITPQMFRMVIGQNHADFSTKQQQDAYEFLIHIMSMIQKDVVKHKNEKIRNPVESTA 186
PN GI P F+ IGQNH +FS+ +QQDA EFL ++ ++ K + I NP +
Sbjct: 410 PN-GIKPTTFKKCIGQNHQEFSSNRQQDAMEFLTFLLDLLDKKFFSSSSSGIPNPNDLVR 468
Query: 187 LRIEDRIFCNTSQTVKYQYRDELVLSLPISLDDAINKDEVRASEAANADNIHSDDVIKPI 366
+EDR+ CN VKY Y + +P+ +N D+++ I
Sbjct: 469 FMMEDRLQCNICGKVKYSYEPTEAIQIPLE------------------ENDEPQDMLERI 510
Query: 367 VTFESCLNKFIQDDVILDYVVNGQASI-AYHSSKLETFPDYLWIQLKRFTVGDDWTPKKL 543
+ + I N + + A ++ P L + R + +W P K
Sbjct: 511 -------KAYFEGQTIEFKCANCKEKVTANKKPGFKSLPQTLILNPIRIRL-QNWIPVKT 562
Query: 544 DVEVLVP------DQLDLSHMKAKGLQP-NEVEMKDEKEPAKAF 654
E+ +P D LD+S ++G P E + DE E +F
Sbjct: 563 SNELSLPGLIDRDDMLDVSSYLSQGFDPQTENLLPDEDENRSSF 606
>sp|Q11119|UBP14_SCHPO Ubiquitin carboxyl-terminal hydrolase 14 (Ubiquitin thiolesterase
14) (Ubiquitin-specific processing protease 14)
(Deubiquitinating enzyme 14) (UBA-domain containing
protein 2)
Length = 775
Score = 67.0 bits (162), Expect = 4e-11
Identities = 58/212 (27%), Positives = 102/212 (48%), Gaps = 5/212 (2%)
Frame = +1
Query: 16 GITPQMFRMVIGQNHADFSTKQQQDAYEFLIHIMSMIQK-DVVKHKNEKIRNPVESTALR 192
G+ P MF+ V+GQ H++F T QQQDAYEFL++++ I+K + K KI +
Sbjct: 384 GLRPFMFKDVVGQGHSEFGTSQQQDAYEFLLYLLGKIRKSSIAKTDITKIFD------FE 437
Query: 193 IEDRIFCNTSQTVKYQYRDELVLSLPISLDDAINKDEVRASEAANADNIHSDDVIKPIVT 372
E ++ C + + V+Y L+L + V+ E + I+ + V+
Sbjct: 438 TEQKLSCLSCKRVRYSSFSSQGLTLTV--------PRVKIGE-IEGEQIYEE------VS 482
Query: 373 FESCLNKFIQDDVILDYVVNGQASI--AYHSSKLETFPDYLWIQLKRFTVGDDWTPKKLD 546
+ CL+ IQ D ++Y S A ++ +++FP L +Q RF + + KKL
Sbjct: 483 IDQCLDATIQPDQ-MEYTCEACKSKLGATTTTAMKSFPKVLILQANRFDL-QGYQVKKLS 540
Query: 547 VEVLVPDQ--LDLSHMKAKGLQPNEVEMKDEK 636
+ ++V + + + AK PN+ + EK
Sbjct: 541 IPIIVNEDGIYNFDRLMAKD-HPNDEDYLPEK 571
>sp|Q80U87|UBP8_MOUSE Ubiquitin carboxyl-terminal hydrolase 8 (Ubiquitin thiolesterase 8)
(Ubiquitin-specific processing protease 8)
(Deubiquitinating enzyme 8) (mUBPy)
Length = 1080
Score = 38.9 bits (89), Expect = 0.012
Identities = 46/199 (23%), Positives = 86/199 (43%), Gaps = 11/199 (5%)
Frame = +1
Query: 19 ITPQMFRMVIGQNHADFSTKQQQDAYEFLIHIMSMIQKDVVKHKNEKIRNPVESTALRIE 198
I+P+ F++ IG+ + F+ QQD+ E L+ +M + +D+ K N K E ++
Sbjct: 807 ISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHEDLNKADNRKRHK--EENNEHLD 864
Query: 199 DRIFCNTSQTVKYQYRDELVLSL-----PISLDDAINKDEVRASEAANADNIHSDDVIKP 363
D + Q + ++++L ++ + R EA ++ K
Sbjct: 865 DLQAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCRRRSRTFEAFMYLSLPLASTSK- 923
Query: 364 IVTFESCLNKFIQDDVILD----YVVNGQA-SIAYHSSKLETFPDYLWIQLKRFTVGDDW 528
T + CL F +++ + D Y + +A + ++ P L + LKRF+ W
Sbjct: 924 -CTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRW 982
Query: 529 TPKKLDVEVLVP-DQLDLS 582
+KL V P + LDLS
Sbjct: 983 -KQKLQTSVDFPLENLDLS 1000
>sp|P40818|UBP8_HUMAN Ubiquitin carboxyl-terminal hydrolase 8 (Ubiquitin thiolesterase 8)
(Ubiquitin-specific processing protease 8)
(Deubiquitinating enzyme 8) (hUBPy)
Length = 1118
Score = 37.0 bits (84), Expect = 0.045
Identities = 51/203 (25%), Positives = 88/203 (43%), Gaps = 15/203 (7%)
Frame = +1
Query: 19 ITPQMFRMVIGQNHADFSTKQQQDAYEFLIHIMSMIQKDVVKHKNEKIRNPVESTALRIE 198
I+P+ F++ IG+ + F+ QQD+ E L+ +M + +D+ K N K R E+
Sbjct: 845 ISPKDFKITIGKINDQFAGYSQQDSQELLLFLMDGLHEDLNKADNRK-RYKEEN-----N 898
Query: 199 DRIFCNTSQTVKYQYRDELVLSLPISLDDAINKDEV---------RASEAANADNIHSDD 351
D + + +Q +L S+ ++L K V R EA ++
Sbjct: 899 DHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTFEAFMYLSLPLAS 958
Query: 352 VIKPIVTFESCLNKFIQDDVILD----YVVNGQA-SIAYHSSKLETFPDYLWIQLKRFTV 516
K T + CL F +++ + D Y + +A + ++ P L + LKRF+
Sbjct: 959 TSK--CTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSY 1016
Query: 517 GDDWTPKKLDVEVLVP-DQLDLS 582
W +KL V P + LDLS
Sbjct: 1017 DGRW-KQKLQTSVDFPLENLDLS 1038
>sp|P35123|UBP4_MOUSE Ubiquitin carboxyl-terminal hydrolase 4 (Ubiquitin thiolesterase 4)
(Ubiquitin-specific processing protease 4)
(Deubiquitinating enzyme 4) (Ubiquitous nuclear protein)
Length = 962
Score = 34.7 bits (78), Expect = 0.22
Identities = 16/54 (29%), Positives = 30/54 (55%)
Frame = +1
Query: 19 ITPQMFRMVIGQNHADFSTKQQQDAYEFLIHIMSMIQKDVVKHKNEKIRNPVES 180
+ P+MF+ +G+ FS QQQD+ E L I+ + +D+ + K + P ++
Sbjct: 370 VAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKPYLEPKDA 423
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.317 0.134 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,122,180
Number of Sequences: 369166
Number of extensions: 1463904
Number of successful extensions: 3650
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3521
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3632
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5462583840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)