Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_I01
(783 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P54611|VATE_DROME Vacuolar ATP synthase subunit E (V-ATP... 230 3e-60
sp|P31402|VATE_MANSE Vacuolar ATP synthase subunit E (V-ATP... 221 2e-57
sp|P36543|VATE_HUMAN Vacuolar ATP synthase subunit E (V-ATP... 214 2e-55
sp|P11019|VATE_BOVIN Vacuolar ATP synthase subunit E (V-ATP... 213 5e-55
sp|Q9U1G5|VATE_HETSC Vacuolar ATP synthase subunit E (V-ATP... 201 2e-51
sp|P50518|VATE_MOUSE Vacuolar ATP synthase subunit E (V-ATP... 192 1e-48
sp|Q01278|VATE_NEUCR Vacuolar ATP synthase subunit E (V-ATP... 154 3e-37
sp|O94072|VATE_CANAL Vacuolar ATP synthase subunit E (V-ATP... 154 3e-37
sp|O00780|VATE_DICDI Vacuolar ATP synthase subunit E (V-ATP... 145 2e-34
sp|Q39258|VATE_ARATH Vacuolar ATP synthase subunit E (V-ATP... 143 6e-34
>sp|P54611|VATE_DROME Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 28 kDa subunit)
Length = 226
Score = 230 bits (587), Expect = 3e-60
Identities = 116/213 (54%), Positives = 156/213 (73%)
Frame = +2
Query: 2 NMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEKKLEQRKKIQHS 181
+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+EK++E +KKIQ S
Sbjct: 14 HMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKKEKQVELQKKIQSS 73
Query: 182 NMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQGLFQLLEAKVI 361
NM+NQARLK+L +++HV VL +A+KRL +T + +Y+ L +LI QGLFQ++E KVI
Sbjct: 74 NMLNQARLKVLKVREDHVSSVLDDARKRLGEVTKNQSEYETVLTKLIVQGLFQIMEPKVI 133
Query: 362 IRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGGVELCVPNGRVKIV 541
+RC D+ LV +L Y+A+ VEL I++ + L D GGVEL NGR+K+
Sbjct: 134 LRCREVDVPLVRNVLPAAVEQYKAQINQNVELFIDEKDFLSADTCGGVELLALNGRIKVP 193
Query: 542 NTLESRLDLISQQLIPEIREILFGKNTNRIFND 640
NTLESRLDLISQQL+PEIR LFG+N NR F D
Sbjct: 194 NTLESRLDLISQQLVPEIRNALFGRNVNRKFTD 226
>sp|P31402|VATE_MANSE Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 28 kDa subunit)
Length = 226
Score = 221 bits (562), Expect = 2e-57
Identities = 114/213 (53%), Positives = 153/213 (71%)
Frame = +2
Query: 2 NMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEKKLEQRKKIQHS 181
+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+EK++E +KKIQ S
Sbjct: 14 HMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKKEKQVELQKKIQSS 73
Query: 182 NMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQGLFQLLEAKVI 361
NM+NQARLK+L +++HV++VL EA+KRL + D + Y + L LI Q LFQL+E V
Sbjct: 74 NMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDIKLYSDLLVTLIVQALFQLVEPTVT 133
Query: 362 IRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGGVELCVPNGRVKIV 541
+R + D LVE +L Y+AK + V L I+ +N LP D GG+EL GR+KI
Sbjct: 134 LRVRQADKALVESLLGRAQQDYKAKIKKDVVLKIDNENFLPPDTCGGIELIAAKGRIKIS 193
Query: 542 NTLESRLDLISQQLIPEIREILFGKNTNRIFND 640
NTLESRL+LI+QQL+PEIR LFG+N NR F D
Sbjct: 194 NTLESRLELIAQQLLPEIRNALFGRNPNRKFTD 226
>sp|P36543|VATE_HUMAN Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 226
Score = 214 bits (546), Expect = 2e-55
Identities = 105/213 (49%), Positives = 158/213 (74%)
Frame = +2
Query: 2 NMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEKKLEQRKKIQHS 181
+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+EK++EQ+KKIQ S
Sbjct: 14 HMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMS 73
Query: 182 NMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQGLFQLLEAKVI 361
N+MNQARLK+L + + + D+L EA++RL + D +Y+ L L+ QGL+QLLE ++I
Sbjct: 74 NLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMI 133
Query: 362 IRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGGVELCVPNGRVKIV 541
+RC + D LV+ + + Y+ T+ V++ I++++ LP+D+AGGVE+ + ++K+
Sbjct: 134 VRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGVEIYNGDRKIKVS 193
Query: 542 NTLESRLDLISQQLIPEIREILFGKNTNRIFND 640
NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 194 NTLESRLDLIAQQMMPEVRGALFGANANRKFLD 226
>sp|P11019|VATE_BOVIN Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 226
Score = 213 bits (542), Expect = 5e-55
Identities = 104/213 (48%), Positives = 157/213 (73%)
Frame = +2
Query: 2 NMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEKKLEQRKKIQHS 181
+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+EK++EQ+KKIQ S
Sbjct: 14 HMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMS 73
Query: 182 NMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQGLFQLLEAKVI 361
N+MNQARLK+L + + + D+L EA++RL + D +Y+ L L+ QGL+QLLE ++I
Sbjct: 74 NLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMI 133
Query: 362 IRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGGVELCVPNGRVKIV 541
+RC + D LV+ + + Y+ T+ V++ I+++ LP+++AGGVE+ + ++K+
Sbjct: 134 VRCRKQDFPLVKAAVQKAIPVYKVATKRDVDVQIDQEAYLPEEIAGGVEIYNGDRKIKVS 193
Query: 542 NTLESRLDLISQQLIPEIREILFGKNTNRIFND 640
NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 194 NTLESRLDLIAQQMMPEVRGALFGANANRKFLD 226
>sp|Q9U1G5|VATE_HETSC Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 226
Score = 201 bits (510), Expect = 2e-51
Identities = 100/213 (46%), Positives = 151/213 (70%)
Frame = +2
Query: 2 NMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEKKLEQRKKIQHS 181
+M++FIEQEA EK +EI++KA+EEF IEK +LVQ QR K+ E++ K+EK++E ++KIQ S
Sbjct: 14 HMMAFIEQEANEKAEEIDAKAEEEFNIEKGKLVQLQRQKIMEYYEKKEKQVELQRKIQRS 73
Query: 182 NMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQGLFQLLEAKVI 361
NM NQ+RLK L + +H+K+VL EA+ L ++ D ++Y LK L+ QGLFQLLE+KV+
Sbjct: 74 NMQNQSRLKCLKARDDHLKNVLEEARANLSKISADRERYPAILKGLLLQGLFQLLESKVV 133
Query: 362 IRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGGVELCVPNGRVKIV 541
+RC + D +V IL EC + + E+ I+ ++ L + AGGVEL +G++++
Sbjct: 134 LRCRKKDEEMVARILPECLEEVQRTWGNRSEVKIDNEHFLSPESAGGVELLAKDGKIRVS 193
Query: 542 NTLESRLDLISQQLIPEIREILFGKNTNRIFND 640
+TLE+RLDLI+ ++ P++R LFG N NR F D
Sbjct: 194 STLEARLDLIADKITPQVRTALFGPNQNRRFFD 226
>sp|P50518|VATE_MOUSE Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 228
Score = 192 bits (487), Expect = 1e-48
Identities = 99/215 (46%), Positives = 154/215 (71%), Gaps = 2/215 (0%)
Frame = +2
Query: 2 NMISFIEQEAKEKVDE-INSKADEEFEIEKSRLVQDQRSKMNEFFSKEEKKL-EQRKKIQ 175
+M++FIEQEA EK +E I++KA+EEF IEK RL++ QR K+ E++ K+EK++ +Q+KKIQ
Sbjct: 14 HMMAFIEQEANEKAEEEIDAKAEEEFNIEKGRLLETQRLKIMEYYEKKEKQIRQQQKKIQ 73
Query: 176 HSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQGLFQLLEAK 355
SN+MNQARLK+L + + + D+L EA++RL + D +Y+ L L+ QGL+QLLE +
Sbjct: 74 MSNLMNQARLKVLRARDDLITDLLNEAKQRLMKVVKDTTRYQVLLDGLVLQGLYQLLEPR 133
Query: 356 VIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGGVELCVPNGRVK 535
+I+RC + D LV+ + + Y+ T+ V++ I+++ ++AGGVE+ + ++K
Sbjct: 134 MIVRCRKQDFPLVKAAVQKAIPMYKIATKRDVDVQIDQEPTCLRNIAGGVEIYNGDRKIK 193
Query: 536 IVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 640
+ NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 194 VSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 228
>sp|Q01278|VATE_NEUCR Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 26 kDa subunit)
Length = 230
Score = 154 bits (388), Expect = 3e-37
Identities = 79/212 (37%), Positives = 132/212 (62%)
Frame = +2
Query: 5 MISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEKKLEQRKKIQHSN 184
M +FI+QEA+EK EI KADEEF IEKS+LV+ + ++ ++K+ K+ + ++I S
Sbjct: 19 MTAFIKQEAEEKAREIQIKADEEFAIEKSKLVRQETDAIDSAYAKKFKQAQMSQQITRST 78
Query: 185 MMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQGLFQLLEAKVII 364
M N+ RL++LG +QE + ++ A +L T+D +YK+ L+ LI +G + + E +++I
Sbjct: 79 MANKTRLRVLGARQELLDEIFEAASAQLGQATHDLGRYKDILRDLILEGFYAMNEPELVI 138
Query: 365 RCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGGVELCVPNGRVKIVN 544
R + D V ++ Y+ KT+ V+ TI+ +N +P+ AGG+ + NG++ I N
Sbjct: 139 RARQADYDAVREAAGWASAQYKHKTDKDVKATIDAENPVPEGSAGGIIIVGGNGKIDIDN 198
Query: 545 TLESRLDLISQQLIPEIREILFGKNTNRIFND 640
T E+RL L+ +P +R+ LFG+N NR F D
Sbjct: 199 TFEARLTLLKDSALPAMRKALFGENPNRKFFD 230
>sp|O94072|VATE_CANAL Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 226
Score = 154 bits (388), Expect = 3e-37
Identities = 80/212 (37%), Positives = 133/212 (62%)
Frame = +2
Query: 5 MISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEKKLEQRKKIQHSN 184
M +FIE+EAKEK EI KADEE+EIEK+ +V+ + + ++ + ++ KK ++I S
Sbjct: 15 MQAFIEKEAKEKAKEIKLKADEEYEIEKASIVRSETAAIDSTYEQKLKKASLAQQITKST 74
Query: 185 MMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQGLFQLLEAKVII 364
+ N+ RL+IL + E + ++ EA+ L +T D ++YK L LI +G+ L+E KV I
Sbjct: 75 IGNKTRLRILSTKDEVLHEIFDEAEAELKKITKDKKQYKPVLVGLIEEGVLALMEPKVSI 134
Query: 365 RCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGGVELCVPNGRVKIVN 544
+ D+ + + + E A +E K + KVE++I+ N L D+AGG+ + +G++++ N
Sbjct: 135 KVREQDVDVAKEAITEAAKNFEEKAKFKVEISIDDKNFLAKDIAGGIVVVNGSGKIEVDN 194
Query: 545 TLESRLDLISQQLIPEIREILFGKNTNRIFND 640
TLE RL ++S++ +P IR LFG +T R F D
Sbjct: 195 TLEERLKILSEEALPAIRLELFGPSTTRKFFD 226
>sp|O00780|VATE_DICDI Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 233
Score = 145 bits (365), Expect = 2e-34
Identities = 82/221 (37%), Positives = 134/221 (60%), Gaps = 9/221 (4%)
Frame = +2
Query: 5 MISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEKKLEQRKKIQHSN 184
M +FI QEA++K +EI +KA +EF EK R+ Q+++ K+ + + K++K +E +KKI SN
Sbjct: 13 MKNFILQEAQDKANEIKTKATQEFTSEKGRIFQNEKIKIIKEYEKKQKLIEVQKKINLSN 72
Query: 185 MMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQGLFQLLEAKVII 364
+N++RL +L ++E ++DV+ EAQK+L +++D KY+ LK LI QG +L E K+ +
Sbjct: 73 ELNKSRLSVLKVREECLRDVIKEAQKKLATISDDKDKYQTILKNLIYQGFVKLNENKIQV 132
Query: 365 RCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPD---------DLAGGVELCV 517
+ D L+E E A+ Y+ ++++++K+ LP GGV L
Sbjct: 133 VGRKEDAGLLEKATTEAAAQYKKNVGKSIDVSVDKERFLPQGPKSDYNGPTCCGGVILSA 192
Query: 518 PNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 640
GR+ NTL+SRL++ QL P IR L+G +T+R F D
Sbjct: 193 LEGRIICKNTLDSRLEICFDQLTPVIRTQLYGASTSRKFFD 233
>sp|Q39258|VATE_ARATH Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 230
Score = 143 bits (360), Expect = 6e-34
Identities = 81/217 (37%), Positives = 129/217 (59%), Gaps = 11/217 (5%)
Frame = +2
Query: 5 MISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEKKLEQRKKIQHSN 184
M+ FI QEA+EK +EI+ A+EEF IEK +LV+ ++ K+ + + K+EK+ + RKKI +S
Sbjct: 13 MVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQDYEKKEKQADVRKKIDYSM 72
Query: 185 MMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQGLFQLLEAKVII 364
+N +R+K+L Q + V + +A K L N++ D YK+ LK LI Q L +L E V++
Sbjct: 73 QLNASRIKVLQAQDDIVNAMKDQAAKDLLNVSRDEYAYKQLLKDLIVQCLLRLKEPSVLL 132
Query: 365 RCCRGDIRLVEVILDECASAYEAKTEMKV-ELTINKDNCLPD----------DLAGGVEL 511
RC D+ LVE +LD+ Y K ++ E+ ++ LP +GGV L
Sbjct: 133 RCREEDLGLVEAVLDDAKEEYAGKAKVHAPEVAVDTKIFLPPPPKSNDPHGLHCSGGVVL 192
Query: 512 CVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNT 622
+G++ NTL++RLD+ + +P IR+ LFG+ T
Sbjct: 193 ASRDGKIVCENTLDARLDVAFRMKLPVIRKSLFGQVT 229
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,329,501
Number of Sequences: 369166
Number of extensions: 1541953
Number of successful extensions: 5821
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5338
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5727
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7357347200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)