Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_H16
(548 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (... 86 6e-17
sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (... 86 8e-17
sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor... 84 2e-16
sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatch... 82 7e-16
sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-mole... 79 6e-15
sp|Q6HA08|ASTL_HUMAN Astacin-like metalloendopeptidase prec... 76 5e-14
sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precurs... 76 5e-14
sp|P42662|ASTL_COTJA Astacin-like metalloendopeptidase 75 1e-13
sp|Q9U3S9|NAS6_CAEEL Zinc metalloproteinase nas-6 precursor... 75 1e-13
sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precurs... 73 4e-13
>sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (Hatching enzyme zinc-protease
HCE 2 subunit) (Choriolysin H 2)
Length = 279
Score = 85.9 bits (211), Expect = 6e-17
Identities = 45/109 (41%), Positives = 65/109 (59%), Gaps = 1/109 (0%)
Frame = +1
Query: 67 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 243
C++F + + +I V S + GCYS LG GG +Q L+L GCMYS G ++HE HALG
Sbjct: 129 CIRFVRRTNEYDFISVVSKN-GCYSELGRKGG-QQELSLNRGGCMYS-GIIQHELNHALG 185
Query: 244 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETHSVMHY 390
F H R DRD+++ + W I+ + F K D ++ PY+ S+MHY
Sbjct: 186 FQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHY 234
>sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (Hatching enzyme zinc-protease
HCE 1 subunit) (Choriolysin H 1)
Length = 270
Score = 85.5 bits (210), Expect = 8e-17
Identities = 44/109 (40%), Positives = 65/109 (59%), Gaps = 1/109 (0%)
Frame = +1
Query: 67 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 243
C++F + + +I V S GCYS LG GG +Q L++ GCMYS G ++HE HALG
Sbjct: 120 CIRFVRRTNEYDFISVVS-KTGCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALG 176
Query: 244 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETHSVMHY 390
F H R DRD+++ + W+ I+ + F K D ++ PY+ S+MHY
Sbjct: 177 FQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHY 225
>sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor (Nematode astacin 4)
Length = 315
Score = 84.3 bits (207), Expect = 2e-16
Identities = 47/113 (41%), Positives = 69/113 (61%), Gaps = 5/113 (4%)
Frame = +1
Query: 67 CVKFSESSASHYIEVQSL--DQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHAL 240
CVKF S + + + D+GCYSL+G TGG KQP++L + GC+ GT+ HE +HA+
Sbjct: 137 CVKFVARDPSKHHDYLWIHPDEGCYSLVGKTGG-KQPVSLDS-GCI-QVGTIVHELMHAV 193
Query: 241 GFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCD---GCEVDGPYETHSVMHY 390
GF H R+DRD++I+V W ++ QF K + +D PY+ S+MHY
Sbjct: 194 GFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHY 246
>sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatching enzyme zinc-protease LCE
subunit) (Choriolysin L)
Length = 271
Score = 82.4 bits (202), Expect = 7e-16
Identities = 44/131 (33%), Positives = 70/131 (53%), Gaps = 1/131 (0%)
Frame = +1
Query: 1 FPNDKKGIVRECLVELQKDLGNCVKF-SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLN 177
+ +D+K +R + E + C+ F ++ Y+ ++ GC S++GY G KQ +
Sbjct: 103 YESDEKETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRF-GCKSMMGYVGD-KQVVV 158
Query: 178 LQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVD 357
LQ GC+ ++HE +HALGF H H R DRD H+ + W+ I+ F K D +
Sbjct: 159 LQRFGCI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLG 217
Query: 358 GPYETHSVMHY 390
PY+ S+MHY
Sbjct: 218 TPYDYGSIMHY 228
>sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-molecule proteinase)
Length = 251
Score = 79.3 bits (194), Expect = 6e-15
Identities = 41/112 (36%), Positives = 66/112 (58%), Gaps = 2/112 (1%)
Frame = +1
Query: 67 CVKF-SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 243
C++F ++ S Y+E+ + GC+S +G G +Q ++LQ GC+Y GT+ HE +HA+G
Sbjct: 91 CIRFVPRTTESDYVEIFTSGSGCWSYVGRISGAQQ-VSLQANGCVYH-GTIIHELMHAIG 148
Query: 244 FMHTHMRKDRDNHINVKWDRILTSHCSQF-VKCDGCEVDGPYETHSVMHYPK 396
F H H R DRDN++ + + + S S F + V Y+ +S+MHY K
Sbjct: 149 FYHEHTRMDRDNYVTINYQNVDPSMTSNFDIDTYSRYVGEDYQYYSIMHYGK 200
>sp|Q6HA08|ASTL_HUMAN Astacin-like metalloendopeptidase precursor (Oocyte astacin)
(Ovastacin)
Length = 431
Score = 76.3 bits (186), Expect = 5e-14
Identities = 43/134 (32%), Positives = 68/134 (50%), Gaps = 1/134 (0%)
Frame = +1
Query: 1 FPNDKKGIVRECLVELQKDLGNCVKF-SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLN 177
+ + ++ E L E ++ C++F + +I + + GC+S +G +GG Q ++
Sbjct: 112 YDEPSRQVILEALAEFERS--TCIRFVTYQDQRDFISIIPM-YGCFSSVGRSGG-MQVVS 167
Query: 178 LQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVD 357
L +G V HE +H LGF H H R DRD +I V W+ IL F+K +
Sbjct: 168 LAPTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSRSSNML 227
Query: 358 GPYETHSVMHYPKL 399
PY+ SVMHY +L
Sbjct: 228 TPYDYSSVMHYGRL 241
>sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precursor (Nematode astacin 13)
Length = 527
Score = 76.3 bits (186), Expect = 5e-14
Identities = 49/127 (38%), Positives = 68/127 (53%), Gaps = 5/127 (3%)
Frame = +1
Query: 25 VRECLVELQKDLGNCVKFSESSASH--YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCM 198
+ E + E +K C+ FS SA YI + D GCYSL+G GG KQP++L + GC+
Sbjct: 217 IAEAIEEYRKK--TCIDFSPKSAGDLDYIHIVP-DDGCYSLVGRIGG-KQPVSLGD-GCI 271
Query: 199 YSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVD---GPYE 369
KG + HE +HA+GF H R DRD ++ + W + QF K +D Y+
Sbjct: 272 -QKGIIIHELMHAVGFFHEQSRADRDEYVKINWSNVEAGLQDQFDKYSLNMIDHLGTKYD 330
Query: 370 THSVMHY 390
SVMHY
Sbjct: 331 YGSVMHY 337
>sp|P42662|ASTL_COTJA Astacin-like metalloendopeptidase
Length = 310
Score = 75.1 bits (183), Expect = 1e-13
Identities = 42/110 (38%), Positives = 62/110 (56%), Gaps = 2/110 (1%)
Frame = +1
Query: 67 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 243
C+ F + Y+ ++S D GC+S G GG Q +++ GCM+ KG ++HE HALG
Sbjct: 35 CINFVKRKTERDYLIIRSAD-GCWSNYGKVGGG-QTVSVMKGGCMW-KGIIQHELDHALG 91
Query: 244 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDG-PYETHSVMHY 390
F+H H R DRD ++ + W+ I + F K + G PY+ SVMHY
Sbjct: 92 FLHEHSRSDRDKYVKIMWEYISPACRPDFRKFENSNNLGLPYDYSSVMHY 141
>sp|Q9U3S9|NAS6_CAEEL Zinc metalloproteinase nas-6 precursor (Nematode astacin 6)
Length = 344
Score = 74.7 bits (182), Expect = 1e-13
Identities = 43/112 (38%), Positives = 62/112 (55%), Gaps = 4/112 (3%)
Frame = +1
Query: 67 CVKFSESSA-SHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 243
C++F + + Y+ + GCYS +G TGG KQ ++L GC + + + HE +H++G
Sbjct: 114 CIRFEKREGQTDYLNIVK-GYGCYSQVGRTGG-KQEISLGR-GCFFHE-IIVHELMHSVG 169
Query: 244 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVD---GPYETHSVMHY 390
F H H R DRD+HI + WD IL SQF K D Y+ S+MHY
Sbjct: 170 FWHEHSRADRDDHIKINWDNILPGMKSQFDKISAVLQDLQGENYDYKSIMHY 221
>sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precursor (Nematode astacin 15)
Length = 571
Score = 73.2 bits (178), Expect = 4e-13
Identities = 42/113 (37%), Positives = 65/113 (57%), Gaps = 5/113 (4%)
Frame = +1
Query: 67 CVKF--SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHAL 240
C+++ E++ +Y+ + D+GCYS++G GG KQ L+L + GC+ KG + HE +HA+
Sbjct: 156 CIRWVPKEAADVNYVHIYP-DRGCYSMVGKMGG-KQSLSLGS-GCI-QKGIILHELMHAV 211
Query: 241 GFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDG---PYETHSVMHY 390
GF H R DRD+HI + W+ I QF K + Y+ S+MHY
Sbjct: 212 GFFHEQSRTDRDDHITIMWNNIQAGMQGQFEKYGHGTIQSLGTGYDYGSIMHY 264
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,640,506
Number of Sequences: 369166
Number of extensions: 1551917
Number of successful extensions: 3304
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3269
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3833118120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)