Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_H10
(774 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P04113|MLRA_PATYE Myosin regulatory light chain A, smoot... 217 3e-56
sp|P04112|MLRB_PATYE Myosin regulatory light chain B, smoot... 208 1e-53
sp|P08051|MLR_SPISA Myosin regulatory light chain, smooth m... 206 4e-53
sp|P24032|MLRN_CHICK Myosin regulatory light chain 2, smoot... 200 3e-51
sp|Q9CQ19|MLRN_MOUSE Myosin regulatory light chain 2, smoot... 199 9e-51
sp|P19105|MLRM_HUMAN Myosin regulatory light chain 2, nonsa... 199 9e-51
sp|P29269|MLRN_PIG Myosin regulatory light chain 2, smooth ... 198 1e-50
sp|P24844|MLRN_HUMAN Myosin regulatory light chain 2, smoot... 198 1e-50
sp|P02612|MLRM_CHICK Myosin regulatory light chain 2, smoot... 197 2e-50
sp|P13832|MLRA_RAT Myosin regulatory light chain 2-A, smoot... 197 3e-50
>sp|P04113|MLRA_PATYE Myosin regulatory light chain A, smooth adductor muscle
Length = 161
Score = 217 bits (552), Expect = 3e-56
Identities = 105/158 (66%), Positives = 127/158 (80%)
Frame = +3
Query: 30 KRAQRATSNVFGMFPQQQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPTDKELKE 209
+RAQRATSNVF PQ+ +QE KEAFTMIDQNRDGFIDI DLK+M++SLGRTP DKEL
Sbjct: 4 ERAQRATSNVFARLPQKLMQEMKEAFTMIDQNRDGFIDINDLKEMFSSLGRTPDDKELTA 63
Query: 210 MLDESPGPLNFTMFINLFGEKLNGTDPEDSLRNAFKMFDANSKEYLEEEYIKDLLENMGD 389
ML E+PGPLNFTMF+++F +KL+GTD E++LRNAF MFD + L EYIKDLLENMGD
Sbjct: 64 MLKEAPGPLNFTMFLSIFSDKLSGTDTEETLRNAFAMFDELDTKKLNIEYIKDLLENMGD 123
Query: 390 NFTPEEIRQTWKEAPLSGGKLDYEAFVRQIKRGKEDES 503
NFT +E+R T+KEAP++GGK DY F IK E+E+
Sbjct: 124 NFTKDEMRMTFKEAPVTGGKFDYVKFTAMIKGSGEEEA 161
>sp|P04112|MLRB_PATYE Myosin regulatory light chain B, smooth adductor muscle
Length = 156
Score = 208 bits (529), Expect = 1e-53
Identities = 101/156 (64%), Positives = 122/156 (78%)
Frame = +3
Query: 36 AQRATSNVFGMFPQQQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPTDKELKEML 215
A +A S V PQ+QIQE KEAFTMIDQNRDGFIDI DLK+M++SLGRTP DKEL ML
Sbjct: 1 ADKAASGVLTKLPQKQIQEMKEAFTMIDQNRDGFIDINDLKEMFSSLGRTPDDKELTAML 60
Query: 216 DESPGPLNFTMFINLFGEKLNGTDPEDSLRNAFKMFDANSKEYLEEEYIKDLLENMGDNF 395
E+PGPLNFTMF+++F +KL+GTD E++LRNAF MFD + L EYIKDLLENMGDNF
Sbjct: 61 KEAPGPLNFTMFLSIFSDKLSGTDTEETLRNAFAMFDELDTKKLNIEYIKDLLENMGDNF 120
Query: 396 TPEEIRQTWKEAPLSGGKLDYEAFVRQIKRGKEDES 503
T +E+R T+KEAP++GGK DY F IK E+E+
Sbjct: 121 TKDEMRMTFKEAPVTGGKFDYVKFTAMIKGSGEEEA 156
>sp|P08051|MLR_SPISA Myosin regulatory light chain, smooth muscle
Length = 160
Score = 206 bits (525), Expect = 4e-53
Identities = 96/158 (60%), Positives = 130/158 (82%)
Frame = +3
Query: 30 KRAQRATSNVFGMFPQQQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPTDKELKE 209
K+A+ ATS+V F Q QIQE KEAFTMIDQNRDG ID+ DLK+MY++LG P D L+
Sbjct: 4 KKAKAATSSVLTKFTQNQIQEMKEAFTMIDQNRDGLIDVSDLKEMYSNLGTAPQDSVLQA 63
Query: 210 MLDESPGPLNFTMFINLFGEKLNGTDPEDSLRNAFKMFDANSKEYLEEEYIKDLLENMGD 389
M+ E+P +NFT F++LF EK++GTDPE++LRNAF+MFD+++ Y+ EEY+KDLLENMGD
Sbjct: 64 MVKEAP-QMNFTGFLSLFSEKMSGTDPEETLRNAFQMFDSDNTGYIPEEYMKDLLENMGD 122
Query: 390 NFTPEEIRQTWKEAPLSGGKLDYEAFVRQIKRGKEDES 503
NF+ +E+RQTWKEAP++GGK+DY AFV +IK ++D++
Sbjct: 123 NFSKDEVRQTWKEAPIAGGKVDYNAFVSKIKGKEQDDA 160
>sp|P24032|MLRN_CHICK Myosin regulatory light chain 2, smooth muscle minor isoform (G1)
(DTNB) (MLC-2) (Isoform L20-B1)
Length = 172
Score = 200 bits (509), Expect = 3e-51
Identities = 95/158 (60%), Positives = 121/158 (76%), Gaps = 1/158 (0%)
Frame = +3
Query: 30 KRAQRATSNVFGMFPQQQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPTDKELKE 209
KR QRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PTD+ L
Sbjct: 13 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDA 72
Query: 210 MLDESPGPLNFTMFINLFGEKLNGTDPEDSLRNAFKMFDANSKEYLEEEYIKDLLENMGD 389
M++E+PGP+NFTMF+ +FGEKLNGTDPED +RNAF FD + +++E+Y+++LL MGD
Sbjct: 73 MMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGFIQEDYLRELLTTMGD 132
Query: 390 NFTPEEIRQTWKEAPL-SGGKLDYEAFVRQIKRGKEDE 500
FT EE+ + ++EAP+ G +Y F R +K G +D+
Sbjct: 133 RFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170
>sp|Q9CQ19|MLRN_MOUSE Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
Length = 172
Score = 199 bits (505), Expect = 9e-51
Identities = 94/158 (59%), Positives = 121/158 (76%), Gaps = 1/158 (0%)
Frame = +3
Query: 30 KRAQRATSNVFGMFPQQQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPTDKELKE 209
KR QRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PTD+ L+
Sbjct: 13 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEG 72
Query: 210 MLDESPGPLNFTMFINLFGEKLNGTDPEDSLRNAFKMFDANSKEYLEEEYIKDLLENMGD 389
M++E+PGP+NFTMF+ +FGEKLNGTDPED +RNAF FD + ++ E+++++LL MGD
Sbjct: 73 MMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGD 132
Query: 390 NFTPEEIRQTWKEAPL-SGGKLDYEAFVRQIKRGKEDE 500
FT EE+ + ++EAP+ G +Y F R +K G +D+
Sbjct: 133 RFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDK 170
>sp|P19105|MLRM_HUMAN Myosin regulatory light chain 2, nonsarcomeric (Myosin RLC)
sp|Q5RC34|MLRM_PONPY Myosin regulatory light chain 2, nonsarcomeric (Myosin RLC)
Length = 171
Score = 199 bits (505), Expect = 9e-51
Identities = 96/161 (59%), Positives = 121/161 (75%), Gaps = 1/161 (0%)
Frame = +3
Query: 21 KGGKRAQRATSNVFGMFPQQQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPTDKE 200
K KR QRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PTD+
Sbjct: 9 KTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEY 68
Query: 201 LKEMLDESPGPLNFTMFINLFGEKLNGTDPEDSLRNAFKMFDANSKEYLEEEYIKDLLEN 380
L M++E+PGP+NFTMF+ +FGEKLNGTDPED +RNAF FD + ++E+Y+++LL
Sbjct: 69 LDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTT 128
Query: 381 MGDNFTPEEIRQTWKEAPL-SGGKLDYEAFVRQIKRGKEDE 500
MGD FT EE+ + ++EAP+ G +Y F R +K G +D+
Sbjct: 129 MGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 169
>sp|P29269|MLRN_PIG Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
(LC20)
Length = 171
Score = 198 bits (504), Expect = 1e-50
Identities = 94/158 (59%), Positives = 120/158 (75%), Gaps = 1/158 (0%)
Frame = +3
Query: 30 KRAQRATSNVFGMFPQQQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPTDKELKE 209
KR QRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PTD+ L+
Sbjct: 12 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEG 71
Query: 210 MLDESPGPLNFTMFINLFGEKLNGTDPEDSLRNAFKMFDANSKEYLEEEYIKDLLENMGD 389
M+ E+PGP+NFTMF+ +FGEKLNGTDPED +RNAF FD + ++ E+++++LL MGD
Sbjct: 72 MMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGD 131
Query: 390 NFTPEEIRQTWKEAPL-SGGKLDYEAFVRQIKRGKEDE 500
FT EE+ + ++EAP+ G +Y F R +K G +D+
Sbjct: 132 RFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDK 169
>sp|P24844|MLRN_HUMAN Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
(LC20)
Length = 172
Score = 198 bits (504), Expect = 1e-50
Identities = 94/158 (59%), Positives = 120/158 (75%), Gaps = 1/158 (0%)
Frame = +3
Query: 30 KRAQRATSNVFGMFPQQQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPTDKELKE 209
KR QRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDL DM ASLG+ PTD+ L+
Sbjct: 13 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEG 72
Query: 210 MLDESPGPLNFTMFINLFGEKLNGTDPEDSLRNAFKMFDANSKEYLEEEYIKDLLENMGD 389
M+ E+PGP+NFTMF+ +FGEKLNGTDPED +RNAF FD + ++ E+++++LL MGD
Sbjct: 73 MMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGD 132
Query: 390 NFTPEEIRQTWKEAPL-SGGKLDYEAFVRQIKRGKEDE 500
FT EE+ + ++EAP+ G +Y F R +K G +D+
Sbjct: 133 RFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDK 170
>sp|P02612|MLRM_CHICK Myosin regulatory light chain 2, smooth muscle major isoform (G1)
(DTNB) (MLC-2) (Isoform L20-A)
Length = 172
Score = 197 bits (502), Expect = 2e-50
Identities = 93/158 (58%), Positives = 120/158 (75%), Gaps = 1/158 (0%)
Frame = +3
Query: 30 KRAQRATSNVFGMFPQQQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPTDKELKE 209
KR QRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDL DM AS+G+ PTD+ L+
Sbjct: 13 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEG 72
Query: 210 MLDESPGPLNFTMFINLFGEKLNGTDPEDSLRNAFKMFDANSKEYLEEEYIKDLLENMGD 389
M+ E+PGP+NFTMF+ +FGEKLNGTDPED +RNAF FD + ++ E+++++LL MGD
Sbjct: 73 MMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGD 132
Query: 390 NFTPEEIRQTWKEAPL-SGGKLDYEAFVRQIKRGKEDE 500
FT EE+ + ++EAP+ G +Y F R +K G +D+
Sbjct: 133 RFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDK 170
>sp|P13832|MLRA_RAT Myosin regulatory light chain 2-A, smooth muscle isoform (Myosin
RLC-A)
Length = 172
Score = 197 bits (500), Expect = 3e-50
Identities = 94/158 (59%), Positives = 120/158 (75%), Gaps = 1/158 (0%)
Frame = +3
Query: 30 KRAQRATSNVFGMFPQQQIQEFKEAFTMIDQNRDGFIDIEDLKDMYASLGRTPTDKELKE 209
KR QRATSNVF MF Q QIQEFKEAF MIDQNRDGFID EDL DM AS+G+ PTD+ L
Sbjct: 13 KRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLDA 72
Query: 210 MLDESPGPLNFTMFINLFGEKLNGTDPEDSLRNAFKMFDANSKEYLEEEYIKDLLENMGD 389
M++E+PGP+NFTMF+ +FGEKLNGTDPED +RNAF FD + ++E+Y+++LL MGD
Sbjct: 73 MMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEAIGTIQEDYLRELLTTMGD 132
Query: 390 NFTPEEIRQTWKEAPL-SGGKLDYEAFVRQIKRGKEDE 500
FT EE+ + ++EAP+ G +Y F R +K G +D+
Sbjct: 133 RFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDK 170
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,953,845
Number of Sequences: 369166
Number of extensions: 1309059
Number of successful extensions: 4914
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4242
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4494
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7212136400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)