Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_G21
(696 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q90734|ACTN4_CHICK Alpha-actinin 4 (Non-muscle alpha-act... 231 1e-60
sp|P57780|ACTN4_MOUSE Alpha-actinin 4 (Non-muscle alpha-act... 231 1e-60
sp|O43707|ACTN4_HUMAN Alpha-actinin 4 (Non-muscle alpha-act... 231 1e-60
sp|Q9Z1P2|ACTN1_RAT Alpha-actinin 1 (Alpha-actinin cytoskel... 230 3e-60
sp|P12814|ACTN1_HUMAN Alpha-actinin 1 (Alpha-actinin cytosk... 229 4e-60
sp|Q7TPR4|ACTN1_MOUSE Alpha-actinin 1 (Alpha-actinin cytosk... 229 4e-60
sp|Q9QXQ0|ACTN4_RAT Alpha-actinin 4 (Non-muscle alpha-actin... 229 7e-60
sp|P05094|ACTN1_CHICK Alpha-actinin 1 (Alpha-actinin cytosk... 227 3e-59
sp|O88990|ACTN3_MOUSE Alpha-actinin 3 (Alpha actinin skelet... 222 6e-58
sp|P18091|ACTN_DROME Alpha-actinin, sarcomeric (F-actin cro... 222 6e-58
>sp|Q90734|ACTN4_CHICK Alpha-actinin 4 (Non-muscle alpha-actinin 4) (F-actin cross linking
protein)
Length = 904
Score = 231 bits (590), Expect = 1e-60
Identities = 114/185 (61%), Positives = 148/185 (80%), Gaps = 3/185 (1%)
Frame = +1
Query: 4 YSMETLRVSWEQLQSSMRRSKSEIEDQILTRDSKGISQSQMDEFRRCFNHFDKNKTRTLE 183
Y+ME +RV WEQL +++ R+ +E+E+QILTRD+KGISQ QM EFR FNHFDK+ L
Sbjct: 720 YTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHCGALG 779
Query: 184 PKEFKACLVSLGCNLKEEK-GEADFAKIMSEVDPNRSGVVTFGAFIDYMAKETTEQDTLE 360
P+EFKACL+SLG +++ ++ G+A+F +IMS VDPN SG VTF AFID+M++ETT+ DT +
Sbjct: 780 PEEFKACLISLGYDVENDRQGDAEFNRIMSLVDPNGSGSVTFQAFIDFMSRETTDTDTAD 839
Query: 361 QILQSFKVLCQDRDYITAEELRRELPSAEAEYCIANMAPY--PDGGEGALDYKLFASSLY 534
Q++ SFKVL D++YITAEELRRELP +AEYCIA MAPY PD GALDYK F+++LY
Sbjct: 840 QVIASFKVLAGDKNYITAEELRRELPPEQAEYCIARMAPYRGPDAAPGALDYKSFSTALY 899
Query: 535 GHSDL 549
G SDL
Sbjct: 900 GESDL 904
>sp|P57780|ACTN4_MOUSE Alpha-actinin 4 (Non-muscle alpha-actinin 4) (F-actin cross linking
protein)
Length = 912
Score = 231 bits (590), Expect = 1e-60
Identities = 113/185 (61%), Positives = 149/185 (80%), Gaps = 3/185 (1%)
Frame = +1
Query: 4 YSMETLRVSWEQLQSSMRRSKSEIEDQILTRDSKGISQSQMDEFRRCFNHFDKNKTRTLE 183
Y+ME +RV WEQL +++ R+ +E+E+QILTRD+KGISQ QM EFR FNHFDK+ L
Sbjct: 728 YTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALG 787
Query: 184 PKEFKACLVSLGCNLKEEK-GEADFAKIMSEVDPNRSGVVTFGAFIDYMAKETTEQDTLE 360
P+EFKACL+SLG +++ ++ G+A+F +IMS VDPN SG+VTF AFID+M++ETT+ DT +
Sbjct: 788 PEEFKACLISLGYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETTDTDTAD 847
Query: 361 QILQSFKVLCQDRDYITAEELRRELPSAEAEYCIANMAPY--PDGGEGALDYKLFASSLY 534
Q++ SFKVL D+++ITAEELRRELP +AEYCIA MAPY PD GALDYK F+++LY
Sbjct: 848 QVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALY 907
Query: 535 GHSDL 549
G SDL
Sbjct: 908 GESDL 912
>sp|O43707|ACTN4_HUMAN Alpha-actinin 4 (Non-muscle alpha-actinin 4) (F-actin cross linking
protein)
Length = 911
Score = 231 bits (589), Expect = 1e-60
Identities = 114/185 (61%), Positives = 149/185 (80%), Gaps = 3/185 (1%)
Frame = +1
Query: 4 YSMETLRVSWEQLQSSMRRSKSEIEDQILTRDSKGISQSQMDEFRRCFNHFDKNKTRTLE 183
Y+ME +RV WEQL +++ R+ +E+E+QILTRD+KGISQ QM EFR FNHFDK+ L
Sbjct: 727 YTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALG 786
Query: 184 PKEFKACLVSLGCNLKEEK-GEADFAKIMSEVDPNRSGVVTFGAFIDYMAKETTEQDTLE 360
P+EFKACL+SLG +++ ++ GEA+F +IMS VDPN SG+VTF AFID+M++ETT+ DT +
Sbjct: 787 PEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTAD 846
Query: 361 QILQSFKVLCQDRDYITAEELRRELPSAEAEYCIANMAPY--PDGGEGALDYKLFASSLY 534
Q++ SFKVL D+++ITAEELRRELP +AEYCIA MAPY PD GALDYK F+++LY
Sbjct: 847 QVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALY 906
Query: 535 GHSDL 549
G SDL
Sbjct: 907 GESDL 911
>sp|Q9Z1P2|ACTN1_RAT Alpha-actinin 1 (Alpha-actinin cytoskeletal isoform) (Non-muscle
alpha-actinin 1) (F-actin cross linking protein)
Length = 892
Score = 230 bits (586), Expect = 3e-60
Identities = 112/185 (60%), Positives = 149/185 (80%), Gaps = 3/185 (1%)
Frame = +1
Query: 4 YSMETLRVSWEQLQSSMRRSKSEIEDQILTRDSKGISQSQMDEFRRCFNHFDKNKTRTLE 183
Y+ME +RV WEQL +++ R+ +E+E+QILTRD+KGISQ QM+EFR FNHFD++ + TL
Sbjct: 708 YTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLG 767
Query: 184 PKEFKACLVSLGCNL-KEEKGEADFAKIMSEVDPNRSGVVTFGAFIDYMAKETTEQDTLE 360
P+EFKACL+SLG ++ + +GEA+FA+IMS VDPNR GVVTF AFID+M++ET + DT +
Sbjct: 768 PEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTAD 827
Query: 361 QILQSFKVLCQDRDYITAEELRRELPSAEAEYCIANMAPY--PDGGEGALDYKLFASSLY 534
Q++ SFK+L D++YIT +ELRRELP +AEYCIA MAPY PD GALDY F+++LY
Sbjct: 828 QVMASFKILAGDKNYITGDELRRELPPDQAEYCIARMAPYAGPDSVPGALDYMSFSTALY 887
Query: 535 GHSDL 549
G SDL
Sbjct: 888 GESDL 892
>sp|P12814|ACTN1_HUMAN Alpha-actinin 1 (Alpha-actinin cytoskeletal isoform) (Non-muscle
alpha-actinin 1) (F-actin cross linking protein)
Length = 892
Score = 229 bits (585), Expect = 4e-60
Identities = 112/185 (60%), Positives = 149/185 (80%), Gaps = 3/185 (1%)
Frame = +1
Query: 4 YSMETLRVSWEQLQSSMRRSKSEIEDQILTRDSKGISQSQMDEFRRCFNHFDKNKTRTLE 183
Y+ME +RV WEQL +++ R+ +E+E+QILTRD+KGISQ QM+EFR FNHFD++ + TL
Sbjct: 708 YTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLG 767
Query: 184 PKEFKACLVSLGCNL-KEEKGEADFAKIMSEVDPNRSGVVTFGAFIDYMAKETTEQDTLE 360
P+EFKACL+SLG ++ + +GEA+FA+IMS VDPNR GVVTF AFID+M++ET + DT +
Sbjct: 768 PEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTAD 827
Query: 361 QILQSFKVLCQDRDYITAEELRRELPSAEAEYCIANMAPY--PDGGEGALDYKLFASSLY 534
Q++ SFK+L D++YIT +ELRRELP +AEYCIA MAPY PD GALDY F+++LY
Sbjct: 828 QVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALY 887
Query: 535 GHSDL 549
G SDL
Sbjct: 888 GESDL 892
>sp|Q7TPR4|ACTN1_MOUSE Alpha-actinin 1 (Alpha-actinin cytoskeletal isoform) (Non-muscle
alpha-actinin 1) (F-actin cross linking protein)
Length = 892
Score = 229 bits (585), Expect = 4e-60
Identities = 112/185 (60%), Positives = 149/185 (80%), Gaps = 3/185 (1%)
Frame = +1
Query: 4 YSMETLRVSWEQLQSSMRRSKSEIEDQILTRDSKGISQSQMDEFRRCFNHFDKNKTRTLE 183
Y+ME +RV WEQL +++ R+ +E+E+QILTRD+KGISQ QM+EFR FNHFD++ + TL
Sbjct: 708 YNMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLG 767
Query: 184 PKEFKACLVSLGCNL-KEEKGEADFAKIMSEVDPNRSGVVTFGAFIDYMAKETTEQDTLE 360
P+EFKACL+SLG ++ + +GEA+FA+IMS VDPNR GVVTF AFID+M++ET + DT +
Sbjct: 768 PEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTAD 827
Query: 361 QILQSFKVLCQDRDYITAEELRRELPSAEAEYCIANMAPY--PDGGEGALDYKLFASSLY 534
Q++ SFK+L D++YIT +ELRRELP +AEYCIA MAPY PD GALDY F+++LY
Sbjct: 828 QVMASFKILAGDKNYITEDELRRELPPDQAEYCIARMAPYAGPDSVPGALDYMSFSTALY 887
Query: 535 GHSDL 549
G SDL
Sbjct: 888 GESDL 892
>sp|Q9QXQ0|ACTN4_RAT Alpha-actinin 4 (Non-muscle alpha-actinin 4) (F-actin cross linking
protein)
Length = 911
Score = 229 bits (583), Expect = 7e-60
Identities = 112/185 (60%), Positives = 147/185 (79%), Gaps = 3/185 (1%)
Frame = +1
Query: 4 YSMETLRVSWEQLQSSMRRSKSEIEDQILTRDSKGISQSQMDEFRRCFNHFDKNKTRTLE 183
Y+ME LRV WEQL +++ R+ +E+E+QILTRD+KGISQ QM EFR FNHFDK+ L
Sbjct: 727 YTMEHLRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALG 786
Query: 184 PKEFKACLVSLGCNLKEEK-GEADFAKIMSEVDPNRSGVVTFGAFIDYMAKETTEQDTLE 360
P+EFK CL+SLG +++ ++ G+A+F +IMS VDPN SG+VTF AFID+M+KETT+ DT +
Sbjct: 787 PEEFKGCLISLGYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSKETTDTDTAD 846
Query: 361 QILQSFKVLCQDRDYITAEELRRELPSAEAEYCIANMAPY--PDGGEGALDYKLFASSLY 534
Q++ SFKVL D+++IT EELR+ELP +AEYCIA MAPY PD GALDYK F+++LY
Sbjct: 847 QVIGSFKVLAGDKNFITVEELRKELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALY 906
Query: 535 GHSDL 549
G SDL
Sbjct: 907 GESDL 911
>sp|P05094|ACTN1_CHICK Alpha-actinin 1 (Alpha-actinin cytoskeletal isoform) (Non-muscle
alpha-actinin 1) (F-actin cross linking protein)
Length = 893
Score = 227 bits (578), Expect = 3e-59
Identities = 111/185 (60%), Positives = 148/185 (80%), Gaps = 3/185 (1%)
Frame = +1
Query: 4 YSMETLRVSWEQLQSSMRRSKSEIEDQILTRDSKGISQSQMDEFRRCFNHFDKNKTRTLE 183
Y+ME +RV WEQL +++ R+ +E+E+QILTRD+KGISQ QM+EFR FNHFD++ + TL
Sbjct: 709 YTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLG 768
Query: 184 PKEFKACLVSLGCNL-KEEKGEADFAKIMSEVDPNRSGVVTFGAFIDYMAKETTEQDTLE 360
P+EFKACL+SLG ++ + +GEA+FA+IMS VDPNR GVVTF AFID+M++ET + DT +
Sbjct: 769 PEEFKACLISLGYDIGNDAQGEAEFARIMSIVDPNRMGVVTFQAFIDFMSRETADTDTAD 828
Query: 361 QILQSFKVLCQDRDYITAEELRRELPSAEAEYCIANMAPY--PDGGEGALDYKLFASSLY 534
Q++ SFK+L D++YIT +ELRRELP +AEYCIA MAPY D GALDY F+++LY
Sbjct: 829 QVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYNGRDAVPGALDYMSFSTALY 888
Query: 535 GHSDL 549
G SDL
Sbjct: 889 GESDL 893
>sp|O88990|ACTN3_MOUSE Alpha-actinin 3 (Alpha actinin skeletal muscle isoform 3) (F-actin
cross linking protein)
Length = 900
Score = 222 bits (566), Expect = 6e-58
Identities = 107/184 (58%), Positives = 142/184 (77%), Gaps = 2/184 (1%)
Frame = +1
Query: 4 YSMETLRVSWEQLQSSMRRSKSEIEDQILTRDSKGISQSQMDEFRRCFNHFDKNKTRTLE 183
YSME +RV WEQL +S+ R+ +E+E+Q+LTRD+KG+SQ Q++EFR FNHFD+ + +E
Sbjct: 721 YSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKRNGMME 780
Query: 184 PKEFKACLVSLGCNLKEEKGEADFAKIMSEVDPNRSGVVTFGAFIDYMAKETTEQDTLEQ 363
P +F+ACL+S+G +L GE +FA+IM+ VDPN +GVVTF AFID+M +ET E DT EQ
Sbjct: 781 PDDFRACLISMGYDL----GEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTAEQ 836
Query: 364 ILQSFKVLCQDRDYITAEELRRELPSAEAEYCIANMAPYPDGG--EGALDYKLFASSLYG 537
++ SFK+L D++YIT EELRRELP+ +AEYCI MAPY G GALDY F+S+LYG
Sbjct: 837 VVASFKILAGDKNYITPEELRRELPAEQAEYCIRRMAPYKGSGAPSGALDYVAFSSALYG 896
Query: 538 HSDL 549
SDL
Sbjct: 897 ESDL 900
>sp|P18091|ACTN_DROME Alpha-actinin, sarcomeric (F-actin cross linking protein)
Length = 924
Score = 222 bits (566), Expect = 6e-58
Identities = 106/185 (57%), Positives = 144/185 (77%), Gaps = 3/185 (1%)
Frame = +1
Query: 4 YSMETLRVSWEQLQSSMRRSKSEIEDQILTRDSKGISQSQMDEFRRCFNHFDKNKTRTLE 183
Y+METLRV WEQL +S+ R+ +E+E+QILTRDSKGISQ Q++EFR FNHFDKN+T L
Sbjct: 740 YTMETLRVGWEQLLTSINRNINEVENQILTRDSKGISQEQLNEFRSSFNHFDKNRTGRLS 799
Query: 184 PKEFKACLVSLGCNL-KEEKGEADFAKIMSEVDPNRSGVVTFGAFIDYMAKETTEQDTLE 360
P+EFK+CLVSLG ++ K+ +G+ DF +I++ VDPN +G V F AF+D+M +E+T+ DT E
Sbjct: 800 PEEFKSCLVSLGYSIGKDRQGDLDFQRILAVVDPNNTGYVHFDAFLDFMTRESTDTDTAE 859
Query: 361 QILQSFKVLCQDRDYITAEELRRELPSAEAEYCIANMAPY--PDGGEGALDYKLFASSLY 534
Q++ SF++L D+ YI +ELRRELP +AEYCI M PY P+G GALDY F+++LY
Sbjct: 860 QVIDSFRILAADKPYILPDELRRELPPDQAEYCIQRMPPYKGPNGVPGALDYMSFSTALY 919
Query: 535 GHSDL 549
G +DL
Sbjct: 920 GETDL 924
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,582,424
Number of Sequences: 369166
Number of extensions: 1306919
Number of successful extensions: 3987
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3755
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3859
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6024953540
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)