Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_E02
(870 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99P58|RB27B_MOUSE Ras-related protein Rab-27B 233 4e-61
sp|P23640|RB27A_RAT Ras-related protein Rab-27A (Rab-27) (G... 232 9e-61
sp|Q9ERI2|RB27A_MOUSE Ras-related protein Rab-27A 231 2e-60
sp|O00194|RB27B_HUMAN Ras-related protein Rab-27B (C25KG) 231 3e-60
sp|Q99P74|RB27B_RAT Ras-related protein Rab-27B 228 1e-59
sp|P51159|RB27A_HUMAN Ras-related protein Rab-27A (Rab-27) ... 228 2e-59
sp|P25228|RAB3_DROME Ras-related protein Rab-3 165 2e-40
sp|Q94986|RAB3_CAEEL Ras-related protein Rab-3 159 7e-39
sp|Q96E17|RAB3C_HUMAN Ras-related protein Rab-3C 159 1e-38
sp|Q63942|RAB3D_RAT GTP-binding protein Rab-3D 157 3e-38
>sp|Q99P58|RB27B_MOUSE Ras-related protein Rab-27B
Length = 218
Score = 233 bits (595), Expect = 4e-61
Identities = 106/201 (52%), Positives = 156/201 (77%)
Frame = +2
Query: 137 SAVGKTSLLHRYTDDEFTAKFVSTVGVDFKQKRIVHNSETENGVLGKSQKLQLQLWDTAG 316
S VGKT+ L+RYTD++F KF++TVG+DF++KR+V++++ +G GK+ K+ LQLWDTAG
Sbjct: 18 SGVGKTTFLYRYTDNKFNPKFITTVGIDFREKRVVYDTQGADGASGKAFKVHLQLWDTAG 77
Query: 317 QERFRSLSMAFFRDAMGFLLVFDVTNEQSFLHIRNWVTLLRSNAYCDTPDMILVGNKIDL 496
QERFRSL+ AFFRDAMGFLL+FD+T++QSFL++RNW++ L++NAYC+ PD++L+GNK DL
Sbjct: 78 QERFRSLTTAFFRDAMGFLLMFDLTSQQSFLNVRNWMSQLQANAYCENPDIVLIGNKADL 137
Query: 497 EEARQVSYKDAEDLAETLGIPYIETSALSAENVEKAFDQIVVMVMKRIERSIDKNILPVR 676
+ R+V+ + A +LAE GIPY ETSA + +NVEK+ + ++ ++MKR+E+ ++K +P
Sbjct: 138 PDQREVNERQARELAEKYGIPYFETSAATGQNVEKSVETLLDLIMKRMEKCVEKTQVPDT 197
Query: 677 RPVGVSLKQKSESQANSNCAC 739
G S K E A CAC
Sbjct: 198 VNGGNSGKLDGEKPAEKKCAC 218
>sp|P23640|RB27A_RAT Ras-related protein Rab-27A (Rab-27) (GTP-binding protein Ram) (Ram
p25)
Length = 221
Score = 232 bits (592), Expect = 9e-61
Identities = 107/204 (52%), Positives = 154/204 (75%), Gaps = 3/204 (1%)
Frame = +2
Query: 137 SAVGKTSLLHRYTDDEFTAKFVSTVGVDFKQKRIVHNSETENGVLGKSQKLQLQLWDTAG 316
S VGKTS+L++YTD +F +KF++TVG+DF++KR+V+ + +G +G+ Q++ LQLWDTAG
Sbjct: 18 SGVGKTSVLYQYTDGKFNSKFITTVGIDFREKRVVYRANGPDGTVGRGQRIHLQLWDTAG 77
Query: 317 QERFRSLSMAFFRDAMGFLLVFDVTNEQSFLHIRNWVTLLRSNAYCDTPDMILVGNKIDL 496
QERFRSL+ AFFRDAMGFLL+FD+TNEQSFL++RNW++ L+ +AYC+ PD++L GNK DL
Sbjct: 78 QERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDL 137
Query: 497 EEARQVSYKDAEDLAETLGIPYIETSALSAENVEKAFDQIVVMVMKRIERSIDKNILP-- 670
E+ R V ++A +LAE GIPY ETSA + N+ +A + ++ ++MKR+ER +DK+ +P
Sbjct: 138 EDQRAVKEEEARELAEKYGIPYFETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEG 197
Query: 671 -VRRPVGVSLKQKSESQANSNCAC 739
VR S Q SE + C C
Sbjct: 198 VVRSNGHTSTDQLSEEKEKGLCGC 221
>sp|Q9ERI2|RB27A_MOUSE Ras-related protein Rab-27A
Length = 221
Score = 231 bits (590), Expect = 2e-60
Identities = 107/204 (52%), Positives = 153/204 (75%), Gaps = 3/204 (1%)
Frame = +2
Query: 137 SAVGKTSLLHRYTDDEFTAKFVSTVGVDFKQKRIVHNSETENGVLGKSQKLQLQLWDTAG 316
S VGKTS+L++YTD +F +KF++TVG+DF++KR+V+ + +G +G+ Q++ LQLWDTAG
Sbjct: 18 SGVGKTSVLYQYTDGKFNSKFITTVGIDFREKRVVYRANGPDGAVGRGQRIHLQLWDTAG 77
Query: 317 QERFRSLSMAFFRDAMGFLLVFDVTNEQSFLHIRNWVTLLRSNAYCDTPDMILVGNKIDL 496
QERFRSL+ AFFRDAMGFLL+FD+TNEQSFL++RNW++ L+ +AYC+ PD++L GNK DL
Sbjct: 78 QERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDL 137
Query: 497 EEARQVSYKDAEDLAETLGIPYIETSALSAENVEKAFDQIVVMVMKRIERSIDKNILP-- 670
E+ R V ++A +LAE GIPY ETSA + N+ A + ++ ++MKR+ER +DK+ +P
Sbjct: 138 EDQRAVKEEEARELAEKYGIPYFETSAANGTNISHAIEMLLDLIMKRMERCVDKSWIPEG 197
Query: 671 -VRRPVGVSLKQKSESQANSNCAC 739
VR S Q SE + C C
Sbjct: 198 VVRSNGHTSADQLSEEKEKGLCGC 221
>sp|O00194|RB27B_HUMAN Ras-related protein Rab-27B (C25KG)
Length = 218
Score = 231 bits (588), Expect = 3e-60
Identities = 105/201 (52%), Positives = 153/201 (76%)
Frame = +2
Query: 137 SAVGKTSLLHRYTDDEFTAKFVSTVGVDFKQKRIVHNSETENGVLGKSQKLQLQLWDTAG 316
S VGKT+ L+RYTD++F KF++TVG+DF++KR+V+N++ NG GK+ K+ LQLWDTAG
Sbjct: 18 SGVGKTTFLYRYTDNKFNPKFITTVGIDFREKRVVYNAQGPNGSSGKAFKVHLQLWDTAG 77
Query: 317 QERFRSLSMAFFRDAMGFLLVFDVTNEQSFLHIRNWVTLLRSNAYCDTPDMILVGNKIDL 496
QERFRSL+ AFFRDAMGFLL+FD+T++QSFL++RNW++ L++NAYC+ PD++L+GNK DL
Sbjct: 78 QERFRSLTTAFFRDAMGFLLMFDLTSQQSFLNVRNWMSQLQANAYCENPDIVLIGNKADL 137
Query: 497 EEARQVSYKDAEDLAETLGIPYIETSALSAENVEKAFDQIVVMVMKRIERSIDKNILPVR 676
+ R+V+ + A +LA+ GIPY ETSA + +NVEKA + ++ ++MKR+E+ ++K +P
Sbjct: 138 PDQREVNERQARELADKYGIPYFETSAATGQNVEKAVETLLDLIMKRMEQCVEKTQIPDT 197
Query: 677 RPVGVSLKQKSESQANSNCAC 739
G S E C C
Sbjct: 198 VNGGNSGNLDGEKPPEKKCIC 218
>sp|Q99P74|RB27B_RAT Ras-related protein Rab-27B
Length = 218
Score = 228 bits (582), Expect = 1e-59
Identities = 105/201 (52%), Positives = 155/201 (77%)
Frame = +2
Query: 137 SAVGKTSLLHRYTDDEFTAKFVSTVGVDFKQKRIVHNSETENGVLGKSQKLQLQLWDTAG 316
S VGKT+ L+RYTD++F KF++TVG+DF++KR+V++++ +G GK+ K+ LQLWDTAG
Sbjct: 18 SGVGKTTFLYRYTDNKFNPKFITTVGIDFREKRVVYDTQGADGSSGKAFKVHLQLWDTAG 77
Query: 317 QERFRSLSMAFFRDAMGFLLVFDVTNEQSFLHIRNWVTLLRSNAYCDTPDMILVGNKIDL 496
QERFRSL+ AFFRDAMGFLL+FD+T++QSFL++RNW++ L++NAYC+ PD++L+GNK DL
Sbjct: 78 QERFRSLTTAFFRDAMGFLLMFDLTSQQSFLNVRNWMSQLQANAYCENPDIVLIGNKADL 137
Query: 497 EEARQVSYKDAEDLAETLGIPYIETSALSAENVEKAFDQIVVMVMKRIERSIDKNILPVR 676
+ R+V+ + A +LAE GIPY ETSA + +NVEK+ + ++ ++MKR+E+ ++K +P
Sbjct: 138 LDQREVNERQARELAEKYGIPYFETSAATGQNVEKSVETLLDLIMKRMEKCVEKTQVPDT 197
Query: 677 RPVGVSLKQKSESQANSNCAC 739
S K E A CAC
Sbjct: 198 VNGVNSGKVDGEKPAEKKCAC 218
>sp|P51159|RB27A_HUMAN Ras-related protein Rab-27A (Rab-27) (GTP-binding protein Ram)
Length = 221
Score = 228 bits (580), Expect = 2e-59
Identities = 107/204 (52%), Positives = 152/204 (74%), Gaps = 3/204 (1%)
Frame = +2
Query: 137 SAVGKTSLLHRYTDDEFTAKFVSTVGVDFKQKRIVHNSETENGVLGKSQKLQLQLWDTAG 316
S VGKTS+L++YTD +F +KF++TVG+DF+ KR+V+ + +G +G+ Q++ LQLWDTAG
Sbjct: 18 SGVGKTSVLYQYTDGKFNSKFITTVGIDFRPKRVVYRASGPDGPVGRGQRIHLQLWDTAG 77
Query: 317 QERFRSLSMAFFRDAMGFLLVFDVTNEQSFLHIRNWVTLLRSNAYCDTPDMILVGNKIDL 496
QERFRSL+ AFFRDAMGFLL+FD+TNEQSFL++RNW++ L+ +AYC+ PD++L GNK DL
Sbjct: 78 QERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDL 137
Query: 497 EEARQVSYKDAEDLAETLGIPYIETSALSAENVEKAFDQIVVMVMKRIERSIDKNILP-- 670
E+ R V ++A LAE GIPY ETSA + N+ +A + ++ ++MKR+ER +DK+ +P
Sbjct: 138 EDQRVVKEEEAIALAEKYGIPYFETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEG 197
Query: 671 -VRRPVGVSLKQKSESQANSNCAC 739
VR S Q SE + C C
Sbjct: 198 VVRSNGHASTDQLSEEKEKGACGC 221
>sp|P25228|RAB3_DROME Ras-related protein Rab-3
Length = 220
Score = 165 bits (417), Expect = 2e-40
Identities = 83/202 (41%), Positives = 132/202 (65%), Gaps = 1/202 (0%)
Frame = +2
Query: 137 SAVGKTSLLHRYTDDEFTAKFVSTVGVDFKQKRIVHNSETENGVLGKSQKLQLQLWDTAG 316
S+VGKTS L RY DD FT+ FVSTVG+DFK K + + + +++LQ+WDTAG
Sbjct: 30 SSVGKTSFLFRYADDSFTSAFVSTVGIDFKVKTVFRHDK----------RVKLQIWDTAG 79
Query: 317 QERFRSLSMAFFRDAMGFLLVFDVTNEQSFLHIRNWVTLLRSNAYCDTPDMILVGNKIDL 496
QER+R+++ A++R AMGF+L++DVTNE SF +++WVT +++ ++ D +ILVGNK D+
Sbjct: 80 QERYRTITTAYYRGAMGFILMYDVTNEDSFNSVQDWVTQIKTYSW-DNAQVILVGNKCDM 138
Query: 497 EEARQVSYKDAEDLAETLGIPYIETSALSAENVEKAFDQIVVMVMKRIERSIDKN-ILPV 673
E+ R +S++ LA+ LG+ + ETSA NV+ F+++V ++ ++ S+D + L
Sbjct: 139 EDQRVISFERGRQLADQLGVEFFETSAKENVNVKAVFERLVDIICDKMSESLDADPTLVG 198
Query: 674 RRPVGVSLKQKSESQANSNCAC 739
G L + + N+NC C
Sbjct: 199 GGQKGQRLTDQPQGTPNANCNC 220
>sp|Q94986|RAB3_CAEEL Ras-related protein Rab-3
Length = 219
Score = 159 bits (403), Expect = 7e-39
Identities = 80/202 (39%), Positives = 131/202 (64%), Gaps = 1/202 (0%)
Frame = +2
Query: 137 SAVGKTSLLHRYTDDEFTAKFVSTVGVDFKQKRIVHNSETENGVLGKSQKLQLQLWDTAG 316
S+VGKTS L RY DD FT+ FVSTVG+DFK K + + +++LQ+WDTAG
Sbjct: 31 SSVGKTSFLFRYCDDSFTSAFVSTVGIDFKVKTVFRGDK----------RVKLQIWDTAG 80
Query: 317 QERFRSLSMAFFRDAMGFLLVFDVTNEQSFLHIRNWVTLLRSNAYCDTPDMILVGNKIDL 496
QER+R+++ A++R AMGF+L++D+TNE+SF +++W T +++ ++ + ++LVGNK D+
Sbjct: 81 QERYRTITTAYYRGAMGFILMYDITNEESFNSVQDWCTQIKTYSW-ENAQVVLVGNKCDM 139
Query: 497 EEARQVSYKDAEDLAETLGIPYIETSALSAENVEKAFDQIVVMVMKRIERSIDKNILPVR 676
+ R VS LA+ LG+ + ETSA NV+ F+++V ++ ++ S+DK+ P +
Sbjct: 140 DSERVVSMDRGRQLADQLGLEFFETSAKENINVKAVFEKLVEIICDKMAESLDKD--PQQ 197
Query: 677 RPVGVSLK-QKSESQANSNCAC 739
+P G L+ ++ A C C
Sbjct: 198 QPKGQKLEANPTQKPAQQQCNC 219
>sp|Q96E17|RAB3C_HUMAN Ras-related protein Rab-3C
Length = 227
Score = 159 bits (401), Expect = 1e-38
Identities = 80/201 (39%), Positives = 129/201 (64%)
Frame = +2
Query: 137 SAVGKTSLLHRYTDDEFTAKFVSTVGVDFKQKRIVHNSETENGVLGKSQKLQLQLWDTAG 316
S+VGKTS L RY DD FT+ FVSTVG+DFK K + N + +++LQ+WDTAG
Sbjct: 39 SSVGKTSFLFRYADDSFTSAFVSTVGIDFKVKTVFKNEK----------RIKLQIWDTAG 88
Query: 317 QERFRSLSMAFFRDAMGFLLVFDVTNEQSFLHIRNWVTLLRSNAYCDTPDMILVGNKIDL 496
QER+R+++ A++R AMGF+L++D+TNE+SF +++W T +++ ++ D +ILVGNK D+
Sbjct: 89 QERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSW-DNAQVILVGNKCDM 147
Query: 497 EEARQVSYKDAEDLAETLGIPYIETSALSAENVEKAFDQIVVMVMKRIERSIDKNILPVR 676
E+ R +S + + L E LG + ETSA NV++ F+++V ++ ++ S++ +
Sbjct: 148 EDERVISTERGQHLGEQLGFEFFETSAKDNINVKQTFERLVDIICDKMSESLETDPAITA 207
Query: 677 RPVGVSLKQKSESQANSNCAC 739
LK+ + NCAC
Sbjct: 208 AKQNTRLKE-TPPPPQPNCAC 227
>sp|Q63942|RAB3D_RAT GTP-binding protein Rab-3D
Length = 219
Score = 157 bits (398), Expect = 3e-38
Identities = 81/201 (40%), Positives = 128/201 (63%)
Frame = +2
Query: 137 SAVGKTSLLHRYTDDEFTAKFVSTVGVDFKQKRIVHNSETENGVLGKSQKLQLQLWDTAG 316
S+VGKTS L RY DD FT FVSTVG+DFK K + + + +++LQ+WDTAG
Sbjct: 31 SSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDK----------RIKLQIWDTAG 80
Query: 317 QERFRSLSMAFFRDAMGFLLVFDVTNEQSFLHIRNWVTLLRSNAYCDTPDMILVGNKIDL 496
QER+R+++ A++R AMGFLL++D+ N++SF +++W T +++ ++ D +ILVGNK DL
Sbjct: 81 QERYRTITTAYYRGAMGFLLMYDIANQESFTAVQDWATQIKTYSW-DNAQVILVGNKCDL 139
Query: 497 EEARQVSYKDAEDLAETLGIPYIETSALSAENVEKAFDQIVVMVMKRIERSIDKNILPVR 676
E+ R VS +D + LA LG + E SA NV++ F+++V ++ ++ S++ + P
Sbjct: 140 EDERVVSAEDGQRLAGDLGFEFFEASAKENINVKQVFERLVDIICDKMNESLEPSSSPGS 199
Query: 677 RPVGVSLKQKSESQANSNCAC 739
G +L Q S+C C
Sbjct: 200 NGKGPALGDTPPPQP-SSCGC 219
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,860,253
Number of Sequences: 369166
Number of extensions: 1758285
Number of successful extensions: 5597
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4849
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8598109270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)