Planarian EST Database


Dr_sW_015_C21

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_015_C21
         (727 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q04831|GLNA_PANAR  Glutamine synthetase (Glutamate--ammon...   378   e-104
sp|P16580|GLNA_CHICK  Glutamine synthetase (Glutamate--ammon...   370   e-102
sp|Q9QY94|GLNA_ACOCA  Glutamine synthetase (Glutamate--ammon...   368   e-101
sp|P15105|GLNA_MOUSE  Glutamine synthetase (Glutamate--ammon...   368   e-101
sp|P15104|GLNA_HUMAN  Glutamine synthetase (Glutamate--ammon...   367   e-101
sp|Q8HZM5|GLNA_CANFA  Glutamine synthetase (Glutamate--ammon...   366   e-101
sp|P09606|GLNA_RAT  Glutamine synthetase (Glutamate--ammonia...   365   e-101
sp|P46410|GLNA_PIG  Glutamine synthetase (Glutamate--ammonia...   365   e-101
sp|Q4R7U3|GLNA_MACFA  Glutamine synthetase (Glutamate--ammon...   359   5e-99
sp|P20478|GLNA2_DROME  Glutamine synthetase 2, cytoplasmic (...   357   1e-98
>sp|Q04831|GLNA_PANAR Glutamine synthetase (Glutamate--ammonia ligase)
          Length = 361

 Score =  378 bits (970), Expect = e-104
 Identities = 169/242 (69%), Positives = 197/242 (81%)
 Frame = +1

Query: 1   KTRTLNFVPVKPKDVPIWNFDGSSTGQAEGSNSDIYLHPVALFNDPFRGKDNKMVLCETY 180
           KTRTLNF P  P ++PIWNFDGSSTGQAE SNSD+YL+PVA++ DPFR  +NK+VLCETY
Sbjct: 40  KTRTLNFTPKSPSELPIWNFDGSSTGQAERSNSDVYLYPVAVYRDPFRLGNNKLVLCETY 99

Query: 181 TYDQTPHDSNLRHSCEEAMKAAEDYHPWFGIEQEYTLLDIDLYPFSWPKLGFPGAQGPYY 360
            Y++ P D+N R  C E M  A D HPWFG+EQEYTLLDID +P  WPK G+PG QGPYY
Sbjct: 100 KYNKKPADTNQRWKCMEVMTRAADQHPWFGMEQEYTLLDIDKHPLGWPKNGYPGPQGPYY 159

Query: 361 CGVGCNKVFGRDIIEAHYKACLFAGIKIAGTNAEVMPAQWEFQIGPCEGINVSDHLWMAR 540
           CGVG N+V+GRD++EAHY+ACL AGI I+G NA+VMPAQWEFQ+GPCEGI + D LWMAR
Sbjct: 160 CGVGANRVYGRDVVEAHYRACLCAGINISGENAKVMPAQWEFQVGPCEGITMGDDLWMAR 219

Query: 541 YLLHRVAEDFGVVVSFDPKPIAGDWNGAGAHTNYSTIQMRSPGGIKYIEEAIEKMSKRHH 720
           YLLHRVAEDF VVV+ DPKPI GDWNGAG HTN+ST  MR P GI  IE AI+K+SK H 
Sbjct: 220 YLLHRVAEDFDVVVTLDPKPIPGDWNGAGMHTNFSTEAMRGPNGILEIESAIDKLSKVHE 279

Query: 721 EH 726
           +H
Sbjct: 280 KH 281
>sp|P16580|GLNA_CHICK Glutamine synthetase (Glutamate--ammonia ligase) (GS)
          Length = 373

 Score =  370 bits (949), Expect = e-102
 Identities = 163/242 (67%), Positives = 194/242 (80%)
 Frame = +1

Query: 1   KTRTLNFVPVKPKDVPIWNFDGSSTGQAEGSNSDIYLHPVALFNDPFRGKDNKMVLCETY 180
           KTRTL+  P   +D+P WNFDGSST QAEGSNSD+YL P A+F DPFR   NK+VLCE +
Sbjct: 43  KTRTLDHEPKSLEDLPEWNFDGSSTFQAEGSNSDMYLRPAAMFRDPFRKDPNKLVLCEVF 102

Query: 181 TYDQTPHDSNLRHSCEEAMKAAEDYHPWFGIEQEYTLLDIDLYPFSWPKLGFPGAQGPYY 360
            Y++   D+NLRH+C   M    + HPWFG+EQEYTLL  D +PF WP   FPG QGPYY
Sbjct: 103 KYNRQSADTNLRHTCRRIMDMVSNQHPWFGMEQEYTLLGTDGHPFGWPSNCFPGPQGPYY 162

Query: 361 CGVGCNKVFGRDIIEAHYKACLFAGIKIAGTNAEVMPAQWEFQIGPCEGINVSDHLWMAR 540
           CGVG +K +GRDI+EAHY+ACL+AG+KI GTNAEVMPAQWEFQ+GPCEGI + DHLW+AR
Sbjct: 163 CGVGADKAYGRDIVEAHYRACLYAGVKIGGTNAEVMPAQWEFQVGPCEGIEMGDHLWIAR 222

Query: 541 YLLHRVAEDFGVVVSFDPKPIAGDWNGAGAHTNYSTIQMRSPGGIKYIEEAIEKMSKRHH 720
           ++LHRV EDFGV+VSFDPKPI G+WNGAG HTN+ST  MR  GG+K+IEEAIEK+SKRH 
Sbjct: 223 FILHRVCEDFGVIVSFDPKPIPGNWNGAGCHTNFSTKNMREDGGLKHIEEAIEKLSKRHQ 282

Query: 721 EH 726
            H
Sbjct: 283 YH 284
>sp|Q9QY94|GLNA_ACOCA Glutamine synthetase (Glutamate--ammonia ligase) (GS)
          Length = 373

 Score =  368 bits (944), Expect = e-101
 Identities = 160/242 (66%), Positives = 196/242 (80%)
 Frame = +1

Query: 1   KTRTLNFVPVKPKDVPIWNFDGSSTGQAEGSNSDIYLHPVALFNDPFRGKDNKMVLCETY 180
           KTRTL+  P   +++P WNFDGSST Q+EGSNSD+YL PVA+F DPFR + NK+V CE +
Sbjct: 43  KTRTLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLSPVAMFRDPFRKEPNKLVFCEVF 102

Query: 181 TYDQTPHDSNLRHSCEEAMKAAEDYHPWFGIEQEYTLLDIDLYPFSWPKLGFPGAQGPYY 360
            Y++ P ++NLRHSC+  M    + HPWFG+EQEYTL+  D +PF WP  GFPG QGPYY
Sbjct: 103 KYNRKPAETNLRHSCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYY 162

Query: 361 CGVGCNKVFGRDIIEAHYKACLFAGIKIAGTNAEVMPAQWEFQIGPCEGINVSDHLWMAR 540
           CGVG +K +GRDI+EAHY+ACL+AG+KI GTNAEVMPAQWEFQIGPCEGI + DHLW+AR
Sbjct: 163 CGVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCEGIRMGDHLWVAR 222

Query: 541 YLLHRVAEDFGVVVSFDPKPIAGDWNGAGAHTNYSTIQMRSPGGIKYIEEAIEKMSKRHH 720
           ++LHRV EDFGV+ +FDPKPI G+WNGAG HTN+ST  MR   G+KYIEEAI+K+SKRH 
Sbjct: 223 FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIDKLSKRHQ 282

Query: 721 EH 726
            H
Sbjct: 283 YH 284
>sp|P15105|GLNA_MOUSE Glutamine synthetase (Glutamate--ammonia ligase) (GS)
          Length = 373

 Score =  368 bits (944), Expect = e-101
 Identities = 160/242 (66%), Positives = 196/242 (80%)
 Frame = +1

Query: 1   KTRTLNFVPVKPKDVPIWNFDGSSTGQAEGSNSDIYLHPVALFNDPFRGKDNKMVLCETY 180
           KTRTL+  P   +++P WNFDGSST Q+EGSNSD+YLHPVA+F DPFR   NK+VLCE +
Sbjct: 43  KTRTLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLHPVAMFRDPFRRDPNKLVLCEVF 102

Query: 181 TYDQTPHDSNLRHSCEEAMKAAEDYHPWFGIEQEYTLLDIDLYPFSWPKLGFPGAQGPYY 360
            Y++ P ++NLRH C+  M    + HPWFG+EQEYTL+  D +PF WP  GFPG QGPYY
Sbjct: 103 KYNRKPAETNLRHICKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYY 162

Query: 361 CGVGCNKVFGRDIIEAHYKACLFAGIKIAGTNAEVMPAQWEFQIGPCEGINVSDHLWMAR 540
           CGVG +K +GRDI+EAHY+ACL+AG+KI GTNAEVMPAQWEFQIGPCEGI + DHLW+AR
Sbjct: 163 CGVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCEGIRMGDHLWIAR 222

Query: 541 YLLHRVAEDFGVVVSFDPKPIAGDWNGAGAHTNYSTIQMRSPGGIKYIEEAIEKMSKRHH 720
           ++LHRV EDFGV+ +FDPKPI G+WNGAG HTN+ST  MR   G+K+IEEAI+K+SKRH 
Sbjct: 223 FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKWIEEAIDKLSKRHQ 282

Query: 721 EH 726
            H
Sbjct: 283 YH 284
>sp|P15104|GLNA_HUMAN Glutamine synthetase (Glutamate--ammonia ligase) (GS)
          Length = 373

 Score =  367 bits (941), Expect = e-101
 Identities = 160/242 (66%), Positives = 197/242 (81%)
 Frame = +1

Query: 1   KTRTLNFVPVKPKDVPIWNFDGSSTGQAEGSNSDIYLHPVALFNDPFRGKDNKMVLCETY 180
           KTRTL+  P   +++P WNFDGSST Q+EGSNSD+YL P A+F DPFR   NK+VLCE +
Sbjct: 43  KTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVF 102

Query: 181 TYDQTPHDSNLRHSCEEAMKAAEDYHPWFGIEQEYTLLDIDLYPFSWPKLGFPGAQGPYY 360
            Y++ P ++NLRH+C+  M    + HPWFG+EQEYTL+  D +PF WP  GFPG QGPYY
Sbjct: 103 KYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYY 162

Query: 361 CGVGCNKVFGRDIIEAHYKACLFAGIKIAGTNAEVMPAQWEFQIGPCEGINVSDHLWMAR 540
           CGVG ++ +GRDI+EAHY+ACL+AG+KIAGTNAEVMPAQWEFQIGPCEGI++ DHLW+AR
Sbjct: 163 CGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVAR 222

Query: 541 YLLHRVAEDFGVVVSFDPKPIAGDWNGAGAHTNYSTIQMRSPGGIKYIEEAIEKMSKRHH 720
           ++LHRV EDFGV+ +FDPKPI G+WNGAG HTN+ST  MR   G+KYIEEAIEK+SKRH 
Sbjct: 223 FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQ 282

Query: 721 EH 726
            H
Sbjct: 283 YH 284
>sp|Q8HZM5|GLNA_CANFA Glutamine synthetase (Glutamate--ammonia ligase) (GS)
          Length = 373

 Score =  366 bits (940), Expect = e-101
 Identities = 160/242 (66%), Positives = 196/242 (80%)
 Frame = +1

Query: 1   KTRTLNFVPVKPKDVPIWNFDGSSTGQAEGSNSDIYLHPVALFNDPFRGKDNKMVLCETY 180
           KTRTL+  P   +++P WNFDGSST Q+EGSNSD+YL P A+F DPFR   NK+V CE +
Sbjct: 43  KTRTLDSEPKGVEELPEWNFDGSSTFQSEGSNSDMYLVPAAMFRDPFRKDPNKLVFCEVF 102

Query: 181 TYDQTPHDSNLRHSCEEAMKAAEDYHPWFGIEQEYTLLDIDLYPFSWPKLGFPGAQGPYY 360
            Y++ P ++NLRH+C+  M    + HPWFG+EQEYTL+  D +PF WP  GFPG QGPYY
Sbjct: 103 KYNRKPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYY 162

Query: 361 CGVGCNKVFGRDIIEAHYKACLFAGIKIAGTNAEVMPAQWEFQIGPCEGINVSDHLWMAR 540
           CGVG +K +GRDI+EAHY+ACL+AGIKIAGTNAEVMPAQWEFQIGPCEGI++ DHLW+AR
Sbjct: 163 CGVGADKAYGRDIVEAHYRACLYAGIKIAGTNAEVMPAQWEFQIGPCEGIDMGDHLWVAR 222

Query: 541 YLLHRVAEDFGVVVSFDPKPIAGDWNGAGAHTNYSTIQMRSPGGIKYIEEAIEKMSKRHH 720
           ++LHRV EDFGV+ +FDPKPI G+WNGAG HTN+ST  MR   G+KYIEE+IEK+SKRH 
Sbjct: 223 FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEESIEKLSKRHQ 282

Query: 721 EH 726
            H
Sbjct: 283 YH 284
>sp|P09606|GLNA_RAT Glutamine synthetase (Glutamate--ammonia ligase) (GS)
          Length = 373

 Score =  365 bits (938), Expect = e-101
 Identities = 160/242 (66%), Positives = 194/242 (80%)
 Frame = +1

Query: 1   KTRTLNFVPVKPKDVPIWNFDGSSTGQAEGSNSDIYLHPVALFNDPFRGKDNKMVLCETY 180
           KTRTL+  P   +++P WNFDGSST Q+EGSNSD+YLHPVA+F DPFR   NK+V CE +
Sbjct: 43  KTRTLDCDPKCVEELPEWNFDGSSTFQSEGSNSDMYLHPVAMFRDPFRRDPNKLVFCEVF 102

Query: 181 TYDQTPHDSNLRHSCEEAMKAAEDYHPWFGIEQEYTLLDIDLYPFSWPKLGFPGAQGPYY 360
            Y++ P ++NLRHSC+  M      HPWFG+EQEYTL+  D +PF WP  GFPG QGPYY
Sbjct: 103 KYNRKPAETNLRHSCKRIMDMVSSQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYY 162

Query: 361 CGVGCNKVFGRDIIEAHYKACLFAGIKIAGTNAEVMPAQWEFQIGPCEGINVSDHLWMAR 540
           CGVG +K +GRDI+EAHY+ACL+AGIKI GTNAEVMPAQWEFQIGPCEGI + DHLW+AR
Sbjct: 163 CGVGADKAYGRDIVEAHYRACLYAGIKITGTNAEVMPAQWEFQIGPCEGIRMGDHLWVAR 222

Query: 541 YLLHRVAEDFGVVVSFDPKPIAGDWNGAGAHTNYSTIQMRSPGGIKYIEEAIEKMSKRHH 720
           ++LHRV EDFGV+ +FDPKPI G+WNGAG HTN+ST  MR   G++ IEEAI+K+SKRH 
Sbjct: 223 FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLRCIEEAIDKLSKRHQ 282

Query: 721 EH 726
            H
Sbjct: 283 YH 284
>sp|P46410|GLNA_PIG Glutamine synthetase (Glutamate--ammonia ligase) (GS)
          Length = 373

 Score =  365 bits (937), Expect = e-101
 Identities = 160/242 (66%), Positives = 195/242 (80%)
 Frame = +1

Query: 1   KTRTLNFVPVKPKDVPIWNFDGSSTGQAEGSNSDIYLHPVALFNDPFRGKDNKMVLCETY 180
           KTRTL+  P   +++P WNFDGSST Q+EGSNSD+YL P A+F DPFR   NK+V CE +
Sbjct: 43  KTRTLDSEPKCIEELPEWNFDGSSTFQSEGSNSDMYLVPAAMFRDPFRKDPNKLVFCEVF 102

Query: 181 TYDQTPHDSNLRHSCEEAMKAAEDYHPWFGIEQEYTLLDIDLYPFSWPKLGFPGAQGPYY 360
            Y++ P ++NLRH+C+  M    + HPWFG+EQEYTL+  D +PF WP  GFPG QGPYY
Sbjct: 103 KYNRKPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYY 162

Query: 361 CGVGCNKVFGRDIIEAHYKACLFAGIKIAGTNAEVMPAQWEFQIGPCEGINVSDHLWMAR 540
           CGVG +K +GRDI+EAHY+ACL+AGIKI GTNAEVMPAQWEFQIGPCEGI++ DHLW+AR
Sbjct: 163 CGVGADKAYGRDIVEAHYRACLYAGIKIGGTNAEVMPAQWEFQIGPCEGIDMGDHLWVAR 222

Query: 541 YLLHRVAEDFGVVVSFDPKPIAGDWNGAGAHTNYSTIQMRSPGGIKYIEEAIEKMSKRHH 720
           ++LHRV EDFGV+ +FDPKPI G+WNGAG HTN+ST  MR   G+KYIEEAIEK+SKRH 
Sbjct: 223 FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQ 282

Query: 721 EH 726
            H
Sbjct: 283 YH 284
>sp|Q4R7U3|GLNA_MACFA Glutamine synthetase (Glutamate--ammonia ligase) (GS)
          Length = 374

 Score =  359 bits (921), Expect = 5e-99
 Identities = 159/243 (65%), Positives = 196/243 (80%), Gaps = 1/243 (0%)
 Frame = +1

Query: 1   KTRTLNFVPVKPKDVPIWNFDGSSTGQAEGSNSDIYLHPVALFNDPFRGKDNKMVLCETY 180
           KTRTL+  P   +++P WNFDGSST Q+EGSNSD+YL P A+F DPFR   NK+VLCE++
Sbjct: 43  KTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCESF 102

Query: 181 TYD-QTPHDSNLRHSCEEAMKAAEDYHPWFGIEQEYTLLDIDLYPFSWPKLGFPGAQGPY 357
               + P ++NLRH+C+  M    + HPWFG+EQEYTL+  D +PF WP  GFPG QGPY
Sbjct: 103 QVQFEGPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPY 162

Query: 358 YCGVGCNKVFGRDIIEAHYKACLFAGIKIAGTNAEVMPAQWEFQIGPCEGINVSDHLWMA 537
           YCGVG ++ +GRDI+EAHY+ACL+AG+KIAGTNAEVMPAQWEFQIGPCEGI++ DHLW+A
Sbjct: 163 YCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVA 222

Query: 538 RYLLHRVAEDFGVVVSFDPKPIAGDWNGAGAHTNYSTIQMRSPGGIKYIEEAIEKMSKRH 717
           R++LHRV EDFGV+ +FDPKPI G+WNGAG HTN+ST  MR   G+KYIEEAIEK+SKRH
Sbjct: 223 RFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRH 282

Query: 718 HEH 726
             H
Sbjct: 283 QYH 285
>sp|P20478|GLNA2_DROME Glutamine synthetase 2, cytoplasmic (Glutamate--ammonia ligase 2)
          Length = 369

 Score =  357 bits (917), Expect = 1e-98
 Identities = 155/242 (64%), Positives = 193/242 (79%)
 Frame = +1

Query: 1   KTRTLNFVPVKPKDVPIWNFDGSSTGQAEGSNSDIYLHPVALFNDPFRGKDNKMVLCETY 180
           K RTL+F+P  PK++P+WN+DGSS  QAEGSNSD YL+PVA++ DPFR  +N +V+C+TY
Sbjct: 49  KDRTLDFIPQSPKELPVWNYDGSSCYQAEGSNSDTYLYPVAIYKDPFRRGNNILVMCDTY 108

Query: 181 TYDQTPHDSNLRHSCEEAMKAAEDYHPWFGIEQEYTLLDIDLYPFSWPKLGFPGAQGPYY 360
            +D TP D+N R +C E         PWFGIEQEYT LD D +P  WPK GFPG QGPYY
Sbjct: 109 KFDGTPTDTNKRKTCLEVANKCAAEEPWFGIEQEYTFLDFDGHPLGWPKNGFPGPQGPYY 168

Query: 361 CGVGCNKVFGRDIIEAHYKACLFAGIKIAGTNAEVMPAQWEFQIGPCEGINVSDHLWMAR 540
           CGVG NKV+ RDI++AHY+ACL+AGIK++GTNAEVMPAQWEFQ+GPCEGI++ D LWMAR
Sbjct: 169 CGVGANKVYARDIVDAHYRACLYAGIKVSGTNAEVMPAQWEFQVGPCEGISIGDDLWMAR 228

Query: 541 YLLHRVAEDFGVVVSFDPKPIAGDWNGAGAHTNYSTIQMRSPGGIKYIEEAIEKMSKRHH 720
           +LLHR++E+FG+V + DPKP+ GDWNGAGAHTN ST  MR  GGI+ IE+A+ K+SK H 
Sbjct: 229 FLLHRISEEFGIVSTLDPKPMPGDWNGAGAHTNVSTKAMREDGGIRDIEKAVAKLSKCHE 288

Query: 721 EH 726
            H
Sbjct: 289 RH 290
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,924,259
Number of Sequences: 369166
Number of extensions: 1988038
Number of successful extensions: 5351
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5095
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5244
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6510836890
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)