Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_C03
(489 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19... 74 2e-13
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 74 2e-13
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 71 2e-12
sp|P28829|BYR2_SCHPO Protein kinase byr2 (Protein kinase st... 70 3e-12
sp|O88506|STK39_RAT STE20/SPS1-related proline-alanine-rich... 70 3e-12
sp|Q9UEW8|STK39_HUMAN STE20/SPS1-related proline-alanine-ri... 70 3e-12
sp|Q9Z1W9|STK39_MOUSE STE20/SPS1-related proline-alanine-ri... 70 3e-12
sp|O95747|OXSR1_HUMAN Serine/threonine-protein kinase OSR1 ... 69 8e-12
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 69 8e-12
sp|Q863I2|OXSR1_PIG Serine/threonine-protein kinase OSR1 (O... 69 8e-12
>sp|Q9SZ67|WRK19_ARATH Probable WRKY transcription factor 19 (WRKY DNA-binding protein 19)
Length = 1895
Score = 73.9 bits (180), Expect = 2e-13
Identities = 50/141 (35%), Positives = 70/141 (49%), Gaps = 4/141 (2%)
Frame = +3
Query: 3 LHRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLQ 182
+HR++ C +VL+D N KL +F L+K + T W+APEV+L
Sbjct: 1746 IHRNIKCANVLVDANGTVKLADFGLAKVMSLWRTPYWN-------------WMAPEVILN 1792
Query: 183 ----ESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSGF 350
+ YG ADIWS G T+LEM+TG+ PY L A + I + + D+
Sbjct: 1793 PKDYDGYGTPADIWSLGCTVLEMLTGQIPYSDLEIGTALYNIGTGKLPKIP-DILSLDAR 1851
Query: 351 DEILDTCLRSNQNERPNVFDL 413
D IL TCL+ N ERP +L
Sbjct: 1852 DFIL-TCLKVNPEERPTAAEL 1871
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 73.6 bits (179), Expect = 2e-13
Identities = 46/139 (33%), Positives = 72/139 (51%), Gaps = 2/139 (1%)
Frame = +3
Query: 3 LHRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLQ 182
+HR + ++L+D KL +F SK + + +T K +KG+ W+APEV+LQ
Sbjct: 193 MHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPY-----WMAPEVILQ 247
Query: 183 ESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAA--HRIVADRHIEFDRDLYPNSGFDE 356
+ F ADIWS G T++EM+TG+ P+ Q AA H H ++ +S ++
Sbjct: 248 TGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNI--SSDAND 305
Query: 357 ILDTCLRSNQNERPNVFDL 413
L CL+ N RP +L
Sbjct: 306 FLLKCLQQEPNLRPTASEL 324
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 70.9 bits (172), Expect = 2e-12
Identities = 44/137 (32%), Positives = 68/137 (49%)
Frame = +3
Query: 3 LHRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLQ 182
+HR + ++L+D KL +F SK + + +T K +KG+ W+APEV+LQ
Sbjct: 194 MHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPY-----WMAPEVILQ 248
Query: 183 ESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSGFDEIL 362
+ F ADIWS G T++EM+TG+ P+ Q AA + +S + L
Sbjct: 249 TGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDAKDFL 308
Query: 363 DTCLRSNQNERPNVFDL 413
CL+ N RP +L
Sbjct: 309 LKCLQEVPNLRPTASEL 325
>sp|P28829|BYR2_SCHPO Protein kinase byr2 (Protein kinase ste8) (MAPK kinase kinase)
(MAPKKK)
Length = 659
Score = 70.1 bits (170), Expect = 3e-12
Identities = 43/137 (31%), Positives = 70/137 (51%)
Frame = +3
Query: 3 LHRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLQ 182
+HR + ++L+D K+ +F +SK L ST G + S + W+APEV+ Q
Sbjct: 519 VHRDIKGANILVDNKGKIKISDFGISKKLELNSTSTKTGGARPS-FQGSSFWMAPEVVKQ 577
Query: 183 ESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEFDRDLYPNSGFDEIL 362
+ K DIWS G ++EM+T + PYP + +QA RI + EF ++ +S + L
Sbjct: 578 TMHTEKTDIWSLGCLVIEMLTSKHPYPNCDQMQAIFRIGENILPEFPSNI--SSSAIDFL 635
Query: 363 DTCLRSNQNERPNVFDL 413
+ + N RP +L
Sbjct: 636 EKTFAIDCNLRPTASEL 652
>sp|O88506|STK39_RAT STE20/SPS1-related proline-alanine-rich protein kinase (Ste-20
related kinase) (Serine/threonine-protein kinase 39)
(Pancreatic serine/threonine-protein kinase) (PS/TK)
(PSTK1)
Length = 553
Score = 70.1 bits (170), Expect = 3e-12
Identities = 44/149 (29%), Positives = 80/149 (53%), Gaps = 12/149 (8%)
Frame = +3
Query: 3 LHRSLNCGSVLIDFNDNAKLCNFHLSKFLF---DCSTEQLLKGVKGSKIEDDECWVAPEV 173
+HR L G++L+ + + ++ +F +S FL D + ++ K G+ CW+APEV
Sbjct: 198 IHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTP-----CWMAPEV 252
Query: 174 LLQ-ESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIV------ADRHIEFDRDL 332
+ Q Y FKAD+WSFG+T +E+ TG PY + ++ + + +E D+++
Sbjct: 253 MEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVE-DKEM 311
Query: 333 YPNSG--FDEILDTCLRSNQNERPNVFDL 413
G F ++L CL+ + ++RP +L
Sbjct: 312 MKKYGKSFRKLLSLCLQKDPSKRPTAAEL 340
>sp|Q9UEW8|STK39_HUMAN STE20/SPS1-related proline-alanine-rich protein kinase (Ste-20
related kinase) (Serine/threonine-protein kinase 39)
(DCHT)
Length = 547
Score = 70.1 bits (170), Expect = 3e-12
Identities = 44/149 (29%), Positives = 80/149 (53%), Gaps = 12/149 (8%)
Frame = +3
Query: 3 LHRSLNCGSVLIDFNDNAKLCNFHLSKFLF---DCSTEQLLKGVKGSKIEDDECWVAPEV 173
+HR L G++L+ + + ++ +F +S FL D + ++ K G+ CW+APEV
Sbjct: 191 IHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTP-----CWMAPEV 245
Query: 174 LLQ-ESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIV------ADRHIEFDRDL 332
+ Q Y FKAD+WSFG+T +E+ TG PY + ++ + + +E D+++
Sbjct: 246 MEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVE-DKEM 304
Query: 333 YPNSG--FDEILDTCLRSNQNERPNVFDL 413
G F ++L CL+ + ++RP +L
Sbjct: 305 MKKYGKSFRKLLSLCLQKDPSKRPTAAEL 333
>sp|Q9Z1W9|STK39_MOUSE STE20/SPS1-related proline-alanine-rich protein kinase (Ste-20
related kinase) (Serine/threonine-protein kinase 39)
Length = 556
Score = 70.1 bits (170), Expect = 3e-12
Identities = 44/149 (29%), Positives = 80/149 (53%), Gaps = 12/149 (8%)
Frame = +3
Query: 3 LHRSLNCGSVLIDFNDNAKLCNFHLSKFLF---DCSTEQLLKGVKGSKIEDDECWVAPEV 173
+HR L G++L+ + + ++ +F +S FL D + ++ K G+ CW+APEV
Sbjct: 201 IHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTP-----CWMAPEV 255
Query: 174 LLQ-ESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIV------ADRHIEFDRDL 332
+ Q Y FKAD+WSFG+T +E+ TG PY + ++ + + +E D+++
Sbjct: 256 MEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVE-DKEM 314
Query: 333 YPNSG--FDEILDTCLRSNQNERPNVFDL 413
G F ++L CL+ + ++RP +L
Sbjct: 315 MKKYGKSFRKLLSLCLQKDPSKRPTAAEL 343
>sp|O95747|OXSR1_HUMAN Serine/threonine-protein kinase OSR1 (Oxidative stress-responsive 1
protein)
Length = 527
Score = 68.6 bits (166), Expect = 8e-12
Identities = 42/148 (28%), Positives = 77/148 (52%), Gaps = 11/148 (7%)
Frame = +3
Query: 3 LHRSLNCGSVLIDFNDNAKLCNFHLSKFLF---DCSTEQLLKGVKGSKIEDDECWVAPEV 173
+HR + G++L+ + + ++ +F +S FL D + ++ K G+ CW+APEV
Sbjct: 143 IHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTP-----CWMAPEV 197
Query: 174 LLQ-ESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEF-----DRDLY 335
+ Q Y FKADIWSFG+T +E+ TG PY + ++ + + D+++
Sbjct: 198 MEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEML 257
Query: 336 PNSG--FDEILDTCLRSNQNERPNVFDL 413
G F +++ CL+ + +RP +L
Sbjct: 258 KKYGKSFRKMISLCLQKDPEKRPTAAEL 285
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 68.6 bits (166), Expect = 8e-12
Identities = 44/139 (31%), Positives = 69/139 (49%), Gaps = 2/139 (1%)
Frame = +3
Query: 3 LHRSLNCGSVLIDFNDNAKLCNFHLSKFLFDCSTEQLLKGVKGSKIEDDECWVAPEVLLQ 182
+HR + ++L+D +L +F SK + + +T K +KG+ W+APEV+LQ
Sbjct: 193 MHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPY-----WMAPEVILQ 247
Query: 183 ESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAA--HRIVADRHIEFDRDLYPNSGFDE 356
+ F ADIWS G T++EM TG+ P+ + AA H H DL P + +
Sbjct: 248 TGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDLSPEA--KD 305
Query: 357 ILDTCLRSNQNERPNVFDL 413
L CL + R + +L
Sbjct: 306 FLMKCLHKEPSLRLSATEL 324
>sp|Q863I2|OXSR1_PIG Serine/threonine-protein kinase OSR1 (Oxidative stress-responsive 1
protein)
Length = 529
Score = 68.6 bits (166), Expect = 8e-12
Identities = 42/148 (28%), Positives = 77/148 (52%), Gaps = 11/148 (7%)
Frame = +3
Query: 3 LHRSLNCGSVLIDFNDNAKLCNFHLSKFLF---DCSTEQLLKGVKGSKIEDDECWVAPEV 173
+HR + G++L+ + + ++ +F +S FL D + ++ K G+ CW+APEV
Sbjct: 143 IHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTP-----CWMAPEV 197
Query: 174 LLQ-ESYGFKADIWSFGVTMLEMMTGRRPYPQLNNVQAAHRIVADRHIEF-----DRDLY 335
+ Q Y FKADIWSFG+T +E+ TG PY + ++ + + D+++
Sbjct: 198 MEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEML 257
Query: 336 PNSG--FDEILDTCLRSNQNERPNVFDL 413
G F +++ CL+ + +RP +L
Sbjct: 258 KKYGKSFRKMISLCLQKDPEKRPTAAEL 285
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,977,529
Number of Sequences: 369166
Number of extensions: 1255617
Number of successful extensions: 5947
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5445
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5632
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2970720600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)