Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_B20-2
(439 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6QAT0|RL32_PIG 60S ribosomal protein L32 >gi|51702810|s... 150 2e-36
sp|Q90YT6|RL32_ICTPU 60S ribosomal protein L32 144 8e-35
sp|Q962T1|RL32_SPOFR 60S ribosomal protein L32 141 5e-34
sp|P84327|RL32_DROPE 60S ribosomal protein L32 (Ribosomal p... 140 2e-33
sp|P17932|RL32P_MOUSE 60S ribosomal protein L32' 140 2e-33
sp|P84311|RL32_DROSU 60S ribosomal protein L32 (Ribosomal p... 140 2e-33
sp|Q94460|RL32_DROAC 60S ribosomal protein L32 (Ribosomal p... 139 2e-33
sp|P61127|RL32_DROYA 60S ribosomal protein L32 (Ribosomal p... 139 3e-33
sp|Q8WRF3|RL32_APIME 60S ribosomal protein L32 (Ribosomal p... 134 9e-32
sp|P79015|RL32A_SCHPO 60S ribosomal protein L32-A 130 2e-30
>sp|Q6QAT0|RL32_PIG 60S ribosomal protein L32
sp|P62911|RL32_MOUSE 60S ribosomal protein L32
sp|P62912|RL32_RAT 60S ribosomal protein L32
sp|P62910|RL32_HUMAN 60S ribosomal protein L32
sp|Q76KA3|RL32_MACFA 60S ribosomal protein L32
Length = 135
Score = 150 bits (378), Expect = 2e-36
Identities = 72/135 (53%), Positives = 99/135 (73%)
Frame = +2
Query: 14 MAVIKPLIKHNIEKKRTKRWVRHHADRFVRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGY 193
MA ++PL+K I KKRTK+++RH +DR+V++ WRKPRGIDNRVRR+FKGQ+ MP IGY
Sbjct: 1 MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGY 60
Query: 194 GTRKVYRNVHKDGFKHFPISNVEELEVLLMSRQSVAAVIDSTVSAKKRVAIIKRAEELEI 373
G+ K +++ GF+ F + NV+ELEVLLM +S A I VS+K R AI++RA +L I
Sbjct: 61 GSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAI 120
Query: 374 RVVNAAAKVKSVETE 418
RV N A+++S E E
Sbjct: 121 RVTNPNARLRSEENE 135
>sp|Q90YT6|RL32_ICTPU 60S ribosomal protein L32
Length = 135
Score = 144 bits (363), Expect = 8e-35
Identities = 71/135 (52%), Positives = 96/135 (71%)
Frame = +2
Query: 14 MAVIKPLIKHNIEKKRTKRWVRHHADRFVRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGY 193
MA ++PL K I KKRTK+++RH +DR+V++ WRKPRGIDNRVR FKGQ+ MP IGY
Sbjct: 1 MAALRPLTKPKIVKKRTKKFIRHQSDRYVKIRRNWRKPRGIDNRVRSGFKGQMLMPNIGY 60
Query: 194 GTRKVYRNVHKDGFKHFPISNVEELEVLLMSRQSVAAVIDSTVSAKKRVAIIKRAEELEI 373
G+ K +++ GFK F + NV+ELEVLLMS +S A I VS+K R I++RA +L I
Sbjct: 61 GSNKKTKHMLPSGFKKFLVHNVKELEVLLMSNKSYCAEIAHNVSSKNRKVIVERAAQLAI 120
Query: 374 RVVNAAAKVKSVETE 418
+V N A+++S E E
Sbjct: 121 KVTNPNARLRSEENE 135
>sp|Q962T1|RL32_SPOFR 60S ribosomal protein L32
Length = 134
Score = 141 bits (356), Expect = 5e-34
Identities = 68/132 (51%), Positives = 95/132 (71%)
Frame = +2
Query: 23 IKPLIKHNIEKKRTKRWVRHHADRFVRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTR 202
I+P+ + I KKRTKR++RH +DR+ +L WRKPRGIDNRVRR+FKGQ MP IGYG+
Sbjct: 3 IRPVYRPTIVKKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGSN 62
Query: 203 KVYRNVHKDGFKHFPISNVEELEVLLMSRQSVAAVIDSTVSAKKRVAIIKRAEELEIRVV 382
K R++ +GF+ + NV ELE+L+M + A I VS+KKR I++RA++L IRV
Sbjct: 63 KKTRHMLPNGFRKVLVHNVRELEILMMQNRKYCAEIAHGVSSKKRKTIVERAQQLSIRVT 122
Query: 383 NAAAKVKSVETE 418
N+AA+++S E E
Sbjct: 123 NSAARLRSQENE 134
>sp|P84327|RL32_DROPE 60S ribosomal protein L32 (Ribosomal protein 49)
sp|P84325|RL32_DROAZ 60S ribosomal protein L32 (Ribosomal protein 49)
sp|P84323|RL32_DROPS 60S ribosomal protein L32 (Ribosomal protein 49)
sp|P84326|RL32_DROMI 60S ribosomal protein L32 (Ribosomal protein 49)
sp|P84324|RL32_DROAI 60S ribosomal protein L32 (Ribosomal protein 49)
Length = 134
Score = 140 bits (352), Expect = 2e-33
Identities = 68/132 (51%), Positives = 91/132 (68%)
Frame = +2
Query: 23 IKPLIKHNIEKKRTKRWVRHHADRFVRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTR 202
I+P + I KKRTK ++RH +DR+ +L HKWRKP+GIDNRVRR+FKGQ MP IGYG+
Sbjct: 3 IRPAYRPKIIKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSN 62
Query: 203 KVYRNVHKDGFKHFPISNVEELEVLLMSRQSVAAVIDSTVSAKKRVAIIKRAEELEIRVV 382
K R++ GFK F + NV ELEVLLM + I VS+KKR I++RA++L IR+
Sbjct: 63 KRTRHMLPTGFKKFLVHNVRELEVLLMQNRVYCGEIAHAVSSKKRKEIVERAKQLSIRLT 122
Query: 383 NAAAKVKSVETE 418
N +++S E E
Sbjct: 123 NPNGRLRSQENE 134
>sp|P17932|RL32P_MOUSE 60S ribosomal protein L32'
Length = 135
Score = 140 bits (352), Expect = 2e-33
Identities = 68/135 (50%), Positives = 94/135 (69%)
Frame = +2
Query: 14 MAVIKPLIKHNIEKKRTKRWVRHHADRFVRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGY 193
MA ++PL+K I KKRTK+++RH +DR+V++ WRKPRGIDNRVRR+FKGQ+ MP IGY
Sbjct: 1 MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKWNWRKPRGIDNRVRRRFKGQILMPNIGY 60
Query: 194 GTRKVYRNVHKDGFKHFPISNVEELEVLLMSRQSVAAVIDSTVSAKKRVAIIKRAEELEI 373
+ K ++ GF+ F + N++E EVLLM +S A I VS+K R AI++RA +L I
Sbjct: 61 RSNKKTKHTLSSGFRKFLVHNIKEPEVLLMCNKSYRAEIAHNVSSKNRKAIVERAAQLAI 120
Query: 374 RVVNAAAKVKSVETE 418
RV N ++ S E E
Sbjct: 121 RVTNPNTRLHSEENE 135
>sp|P84311|RL32_DROSU 60S ribosomal protein L32 (Ribosomal protein 49)
sp|P84313|RL32_DROMD 60S ribosomal protein L32 (Ribosomal protein 49)
sp|P84314|RL32_DROGU 60S ribosomal protein L32 (Ribosomal protein 49)
sp|P84312|RL32_DROBF 60S ribosomal protein L32 (Ribosomal protein 49)
Length = 134
Score = 140 bits (352), Expect = 2e-33
Identities = 68/132 (51%), Positives = 91/132 (68%)
Frame = +2
Query: 23 IKPLIKHNIEKKRTKRWVRHHADRFVRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTR 202
I+P + I KKRTK ++RH +DR+ +L HKWRKP+GIDNRVRR+FKGQ MP IGYG+
Sbjct: 3 IRPAYRPKIIKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSN 62
Query: 203 KVYRNVHKDGFKHFPISNVEELEVLLMSRQSVAAVIDSTVSAKKRVAIIKRAEELEIRVV 382
K R++ GFK F + NV ELEVLLM + I VS+KKR I++RA++L IR+
Sbjct: 63 KRTRHMLPTGFKKFLVHNVRELEVLLMQNRIYCGEIAHAVSSKKRKEIVERAKQLSIRLT 122
Query: 383 NAAAKVKSVETE 418
N +++S E E
Sbjct: 123 NPNGRLRSQENE 134
>sp|Q94460|RL32_DROAC 60S ribosomal protein L32 (Ribosomal protein 49)
Length = 134
Score = 139 bits (351), Expect = 2e-33
Identities = 67/132 (50%), Positives = 91/132 (68%)
Frame = +2
Query: 23 IKPLIKHNIEKKRTKRWVRHHADRFVRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTR 202
I+P + I KKRTK ++RH +DR+ +L HKWRKP+GIDNRVRR+FKGQ MP IGYG+
Sbjct: 3 IRPAYRPKIVKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSN 62
Query: 203 KVYRNVHKDGFKHFPISNVEELEVLLMSRQSVAAVIDSTVSAKKRVAIIKRAEELEIRVV 382
K R++ GFK F + NV ELEVLLM + I VS+ KR I++RA++L +R+
Sbjct: 63 KRTRHMLPTGFKKFLVHNVRELEVLLMQNREYCGEIAHGVSSNKRKEIVERAKQLSVRLT 122
Query: 383 NAAAKVKSVETE 418
N A+++S E E
Sbjct: 123 NPNARLRSQENE 134
>sp|P61127|RL32_DROYA 60S ribosomal protein L32 (Ribosomal protein 49)
sp|P61128|RL32_DROSI 60S ribosomal protein L32 (Ribosomal protein 49)
sp|P04359|RL32_DROME 60S ribosomal protein L32 (Ribosomal protein 49)
Length = 134
Score = 139 bits (350), Expect = 3e-33
Identities = 67/132 (50%), Positives = 91/132 (68%)
Frame = +2
Query: 23 IKPLIKHNIEKKRTKRWVRHHADRFVRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTR 202
I+P + I KKRTK ++RH +DR+ +L HKWRKP+GIDNRVRR+FKGQ MP IGYG+
Sbjct: 3 IRPAYRPKIVKKRTKHFIRHQSDRYAKLSHKWRKPKGIDNRVRRRFKGQYLMPNIGYGSN 62
Query: 203 KVYRNVHKDGFKHFPISNVEELEVLLMSRQSVAAVIDSTVSAKKRVAIIKRAEELEIRVV 382
K R++ GFK F + NV ELEVLLM + I VS+KKR I++RA++L +R+
Sbjct: 63 KRTRHMLPTGFKKFLVHNVRELEVLLMQNRVYCGEIAHGVSSKKRKEIVERAKQLSVRLT 122
Query: 383 NAAAKVKSVETE 418
N +++S E E
Sbjct: 123 NPNGRLRSQENE 134
>sp|Q8WRF3|RL32_APIME 60S ribosomal protein L32 (Ribosomal protein 49)
Length = 134
Score = 134 bits (337), Expect = 9e-32
Identities = 65/132 (49%), Positives = 94/132 (71%)
Frame = +2
Query: 23 IKPLIKHNIEKKRTKRWVRHHADRFVRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTR 202
I+P+ + I KKRTK+++RH +DR+ +L WRKP+GIDNRVRR+FKGQ MP IGYG+
Sbjct: 3 IRPVYRPTIVKKRTKKFIRHQSDRYSKLKRNWRKPKGIDNRVRRRFKGQYLMPNIGYGSN 62
Query: 203 KVYRNVHKDGFKHFPISNVEELEVLLMSRQSVAAVIDSTVSAKKRVAIIKRAEELEIRVV 382
K R++ GF+ + NV+ELEVL+M + A I S+KKR +I++RA++L IRV
Sbjct: 63 KKTRHMLPTGFRKVLVHNVKELEVLMMQNRKFCAEIAHGGSSKKRKSIVERAQQLSIRVT 122
Query: 383 NAAAKVKSVETE 418
A+A+++S E E
Sbjct: 123 YASARLRSQENE 134
>sp|P79015|RL32A_SCHPO 60S ribosomal protein L32-A
Length = 127
Score = 130 bits (326), Expect = 2e-30
Identities = 65/123 (52%), Positives = 86/123 (69%)
Frame = +2
Query: 44 NIEKKRTKRWVRHHADRFVRLGHKWRKPRGIDNRVRRKFKGQVRMPKIGYGTRKVYRNVH 223
NI KKRTK + RH +DRF R+G WRKPRGID+ VRR+F+G + MPKIGYG K R
Sbjct: 5 NIIKKRTKPFKRHQSDRFKRVGESWRKPRGIDSCVRRRFRGTISMPKIGYGNNKKTRYCM 64
Query: 224 KDGFKHFPISNVEELEVLLMSRQSVAAVIDSTVSAKKRVAIIKRAEELEIRVVNAAAKVK 403
+G K F + NV ++E+LLM ++ AA I VSA+KRV I+++A L ++V NA AKV+
Sbjct: 65 PNGLKAFLVRNVSDVELLLMHNKTYAAEIAGNVSARKRVEIVEKARALGVKVTNAGAKVR 124
Query: 404 SVE 412
S E
Sbjct: 125 SQE 127
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,864,590
Number of Sequences: 369166
Number of extensions: 859644
Number of successful extensions: 2449
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2387
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2434
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2244666600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)