Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_A06
(866 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O18973|RABX5_BOVIN Rab5 GDP/GTP exchange factor (Rabex-5) 63 9e-10
sp|Q9UJ41|RABX5_HUMAN Rab5 GDP/GTP exchange factor (Rabex-5) 61 5e-09
sp|Q9JM13|RABX5_MOUSE Rab5 GDP/GTP exchange factor (Rabex-5) 60 6e-09
sp|P54787|VPS9_YEAST Vacuolar protein sorting-associated pr... 52 2e-06
sp|Q5BIW4|ALS2_PANTR Alsin (Amyotrophic lateral sclerosis p... 44 4e-04
sp|Q96Q42|ALS2_HUMAN Alsin (Amyotrophic lateral sclerosis p... 44 4e-04
sp|P59729|RIN3_MOUSE Ras and Rab interactor 3 (Ras interact... 43 0.001
sp|Q920R0|ALS2_MOUSE Alsin (Amyotrophic lateral sclerosis p... 41 0.004
sp|Q8TB24|RIN3_HUMAN Ras and Rab interactor 3 (Ras interact... 40 0.011
sp|P18140|NCAP_TACV Nucleocapsid protein (Nucleoprotein) 38 0.032
>sp|O18973|RABX5_BOVIN Rab5 GDP/GTP exchange factor (Rabex-5)
Length = 492
Score = 63.2 bits (152), Expect = 9e-10
Identities = 47/200 (23%), Positives = 94/200 (47%), Gaps = 5/200 (2%)
Frame = +3
Query: 282 DLSSESESRAQMVSDMLDWASRILNSQPYLNSMTPIRQQETRVYLERMVMSQLHRSSIIL 461
DLS E +S D+ + + P R ++ +E+ +M++L++ +
Sbjct: 173 DLSIEEQSECTQ-----DFYQNVAERMQTRGKVPPERVEKIMDQIEKYIMTRLYKY-VFC 226
Query: 462 NDDIAEVDRDNVFHRELEYLQKVITPYQLSI--NKKFHSLLPFEV-AQKELLKINCYKTP 632
+ + +D + + L V TP L + N++ + V A ++++++ + P
Sbjct: 227 PETTDDEKKDLAIQKRIRALHWV-TPQMLCVPVNEEIPEVSDMVVKAITDIIEMDSKRVP 285
Query: 633 HDKVFCIQKTTHKIAILLKMINSDFIPGADDILPVLIYVIIMANPPRMLSNMSFIDVLC- 809
DK+ CI K + I +K+ ++ ADD LP LIY+++ NPPR+ SN+ +I C
Sbjct: 286 RDKLACITKCSKHIFNAIKITKNE-PASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCN 344
Query: 810 -GNQLEGQNQYMWCQFSAAI 866
+ G++ Y + A+
Sbjct: 345 PSRLMTGEDGYYFTNLCCAV 364
>sp|Q9UJ41|RABX5_HUMAN Rab5 GDP/GTP exchange factor (Rabex-5)
Length = 708
Score = 60.8 bits (146), Expect = 5e-09
Identities = 42/174 (24%), Positives = 88/174 (50%), Gaps = 5/174 (2%)
Frame = +3
Query: 360 QPYLNSMTPIRQQETRVYLERMVMSQLHRSSIILNDDIAEVDRDNVFHRELEYLQKVITP 539
+P ++ P R ++ +E+ +M++L++ + + + +D + + L+ V TP
Sbjct: 410 EPPHPAVPPERVEKIMDQIEKYIMTRLYKY-VFCPETTDDEKKDLAIQKRIRALRWV-TP 467
Query: 540 YQLSI--NKKFHSLLPFEV-AQKELLKINCYKTPHDKVFCIQKTTHKIAILLKMINSDFI 710
L + N+ + V A ++++++ + P DK+ CI K + I +K+ ++
Sbjct: 468 QMLCVPVNEDIPEVSDMVVKAITDIIEMDSKRVPRDKLACITKCSKHIFNAIKITKNE-P 526
Query: 711 PGADDILPVLIYVIIMANPPRMLSNMSFIDVLC--GNQLEGQNQYMWCQFSAAI 866
ADD LP LIY+++ NPPR+ SN+ +I C + G++ Y + A+
Sbjct: 527 ASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAV 580
>sp|Q9JM13|RABX5_MOUSE Rab5 GDP/GTP exchange factor (Rabex-5)
Length = 491
Score = 60.5 bits (145), Expect = 6e-09
Identities = 45/200 (22%), Positives = 94/200 (47%), Gaps = 5/200 (2%)
Frame = +3
Query: 282 DLSSESESRAQMVSDMLDWASRILNSQPYLNSMTPIRQQETRVYLERMVMSQLHRSSIIL 461
DLS E +S D+ + + P + ++ +E+ +M++L++ +
Sbjct: 172 DLSIEEQSECTQ-----DFYQNVAERMQTRGKVPPEKVEKIMDQIEKHIMTRLYKF-VFC 225
Query: 462 NDDIAEVDRDNVFHRELEYLQKVITPYQLSI--NKKFHSLLPFEV-AQKELLKINCYKTP 632
+ + +D + + L V TP L + N++ + V A ++++++ + P
Sbjct: 226 PETTDDEKKDLAIQKRIRALHWV-TPQMLCVPVNEEIPEVSDMVVKAITDIIEMDSKRVP 284
Query: 633 HDKVFCIQKTTHKIAILLKMINSDFIPGADDILPVLIYVIIMANPPRMLSNMSFIDVLC- 809
DK+ CI + + I +K+ ++ ADD LP LIY+++ NPPR+ SN+ +I C
Sbjct: 285 RDKLACITRCSKHIFNAIKITKNE-PASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCN 343
Query: 810 -GNQLEGQNQYMWCQFSAAI 866
+ G++ Y + A+
Sbjct: 344 PSRLMTGEDGYYFTNLCCAV 363
>sp|P54787|VPS9_YEAST Vacuolar protein sorting-associated protein VPS9
Length = 451
Score = 52.4 bits (124), Expect = 2e-06
Identities = 32/94 (34%), Positives = 52/94 (55%), Gaps = 1/94 (1%)
Frame = +3
Query: 588 VAQKELLKINCYKTPHDKVFCIQKTTHKIAILLKMINSDFIPGADDILPVLIYVIIMANP 767
+A KEL KIN +K+P DK+ C+ + I LLK + GAD +PVLIY I+
Sbjct: 208 LASKELGKINRFKSPRDKMVCVLNASKVIFGLLKHTKLE-QNGADSFIPVLIYCILKGQV 266
Query: 768 PRMLSNMSFID-VLCGNQLEGQNQYMWCQFSAAI 866
++SN+++I+ + + G+ +Y AA+
Sbjct: 267 RYLVSNVNYIERFRSPDFIRGEEEYYLSSLQAAL 300
>sp|Q5BIW4|ALS2_PANTR Alsin (Amyotrophic lateral sclerosis protein 2 homolog)
Length = 1657
Score = 44.3 bits (103), Expect = 4e-04
Identities = 34/113 (30%), Positives = 57/113 (50%), Gaps = 7/113 (6%)
Frame = +3
Query: 519 LQKVITPYQLSINKKFHSLLP------FEVAQKELLKINCYKTPHDKVFCIQKTTHKIA- 677
+Q+ P LSI + +LP F A + L +I+ TP DK+ IQ+T +I+
Sbjct: 1525 VQRKFWPATLSILGESKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQ 1584
Query: 678 ILLKMINSDFIPGADDILPVLIYVIIMANPPRMLSNMSFIDVLCGNQLEGQNQ 836
+L ++ DF+ DD+ PV +YV++ A + S + I+ L L+ Q
Sbjct: 1585 SVLASLHEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQ 1637
>sp|Q96Q42|ALS2_HUMAN Alsin (Amyotrophic lateral sclerosis protein 2)
Length = 1657
Score = 44.3 bits (103), Expect = 4e-04
Identities = 34/113 (30%), Positives = 57/113 (50%), Gaps = 7/113 (6%)
Frame = +3
Query: 519 LQKVITPYQLSINKKFHSLLP------FEVAQKELLKINCYKTPHDKVFCIQKTTHKIA- 677
+Q+ P LSI + +LP F A + L +I+ TP DK+ IQ+T +I+
Sbjct: 1525 VQRKFWPATLSILGESKKVLPTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQ 1584
Query: 678 ILLKMINSDFIPGADDILPVLIYVIIMANPPRMLSNMSFIDVLCGNQLEGQNQ 836
+L ++ DF+ DD+ PV +YV++ A + S + I+ L L+ Q
Sbjct: 1585 SVLASLHEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDLMDPYLQHGEQ 1637
>sp|P59729|RIN3_MOUSE Ras and Rab interactor 3 (Ras interaction/interference protein 3)
Length = 980
Score = 43.1 bits (100), Expect = 0.001
Identities = 46/193 (23%), Positives = 84/193 (43%), Gaps = 9/193 (4%)
Frame = +3
Query: 288 SSESESRAQMVSDMLDWASRILNSQPYLNSMTPIRQQETRVYLERMVMSQLHRSSI---- 455
+S +E ++ + M S +L S + P E LE +V S L++ +
Sbjct: 632 TSSTEMLQEIRTMMTQLKSYLLQSTELKALVEPTLHSEEE--LEAIVESALYKCVLKPLK 689
Query: 456 -ILNDDIAEVDRDNVFHRELEYLQKVI---TPYQLSINKKFHSLLPFEVAQKELLKINCY 623
+N + E+ + ++L+ Q V+ T L + + E ++L ++
Sbjct: 690 EAINSSLLEIHSRDGSLQQLKENQLVVLATTTTDLGVTTSVPEVAVMEKILQKLTSMHKA 749
Query: 624 KTPHDKVFCIQKTTHKIAILLKMINSDFIPGADDILPVLIYVIIMANPPRMLSNMSFIDV 803
+P K+ + KT I + + N GADD LPVL+YV+ +N ML N+ ++
Sbjct: 750 YSPGKKISILLKTCKLIYDSMALGNPGKPYGADDFLPVLMYVLARSNLTEMLLNVEYMME 809
Query: 804 LCGNQLE-GQNQY 839
L L+ G+ Y
Sbjct: 810 LMDPALQLGEGSY 822
>sp|Q920R0|ALS2_MOUSE Alsin (Amyotrophic lateral sclerosis protein 2 homolog)
Length = 1651
Score = 41.2 bits (95), Expect = 0.004
Identities = 32/103 (31%), Positives = 52/103 (50%), Gaps = 7/103 (6%)
Frame = +3
Query: 519 LQKVITPYQLSINKKFHSLLP------FEVAQKELLKINCYKTPHDKVFCIQKTTHKIA- 677
+QK P LSI + +L F A + L +I+ TP DK+ IQ+T +I+
Sbjct: 1519 VQKKFWPATLSILGESKKVLSTTKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQ 1578
Query: 678 ILLKMINSDFIPGADDILPVLIYVIIMANPPRMLSNMSFIDVL 806
+L + DF+ DD+ PV +YV++ A + S + I+ L
Sbjct: 1579 SVLASLQEDFLWSMDDLFPVFLYVVLRARIRNLGSEVHLIEDL 1621
>sp|Q8TB24|RIN3_HUMAN Ras and Rab interactor 3 (Ras interaction/interference protein 3)
Length = 984
Score = 39.7 bits (91), Expect = 0.011
Identities = 45/193 (23%), Positives = 83/193 (43%), Gaps = 9/193 (4%)
Frame = +3
Query: 288 SSESESRAQMVSDMLDWASRILNSQPYLNSMTPIRQQETRVYLERMVMSQLHRSSI---- 455
+S +E ++ + M S +L S + P E LE +V S L++ +
Sbjct: 635 TSSTEMLQEIRTMMTQLKSYLLQSTELKALVDPALHSEEE--LEAIVESALYKCVLKPLK 692
Query: 456 -ILNDDIAEVDRDNVFHRELEYLQKVI---TPYQLSINKKFHSLLPFEVAQKELLKINCY 623
+N + ++ + ++L+ Q VI T L + + E ++ ++
Sbjct: 693 EAINSCLHQIHSKDGSLQQLKENQLVILATTTTDLGVTTSVPEVPMMEKILQKFTSMHKA 752
Query: 624 KTPHDKVFCIQKTTHKIAILLKMINSDFIPGADDILPVLIYVIIMANPPRMLSNMSFIDV 803
+P K+ + KT I + + N GADD LPVL+YV+ +N ML N+ ++
Sbjct: 753 YSPEKKISILLKTCKLIYDSMALGNPGKPYGADDFLPVLMYVLARSNLTEMLLNVEYMME 812
Query: 804 LCGNQLE-GQNQY 839
L L+ G+ Y
Sbjct: 813 LMDPALQLGEGSY 825
>sp|P18140|NCAP_TACV Nucleocapsid protein (Nucleoprotein)
Length = 570
Score = 38.1 bits (87), Expect = 0.032
Identities = 32/117 (27%), Positives = 58/117 (49%)
Frame = +3
Query: 453 IILNDDIAEVDRDNVFHRELEYLQKVITPYQLSINKKFHSLLPFEVAQKELLKINCYKTP 632
+I+ I+E+D N ++E LQ + P +L++ ++ A+K+ I+C++ P
Sbjct: 368 LIVRAAISELDPSNTIWLDIEDLQ--LDPVELAL---------YQPAKKQY--IHCFRKP 414
Query: 633 HDKVFCIQKTTHKIAILLKMINSDFIPGADDILPVLIYVIIMANPPRMLSNMSFIDV 803
HD+ + H IL+K I D +PG VL YVI + P +++ D+
Sbjct: 415 HDEKGFKNGSRHSHGILMKDI-EDAVPG------VLSYVIGLLPPNMVITTQGSDDI 464
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,238,478
Number of Sequences: 369166
Number of extensions: 1660604
Number of successful extensions: 4037
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3841
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4025
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8550075140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)