Planarian EST Database


Dr_sW_015_A01

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_015_A01
         (472 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8CST4|IF2_STAES  Translation initiation factor IF-2 >gi|...    42   7e-04
sp|P38691|KSP1_YEAST  Serine/threonine-protein kinase KSP1         42   0.001
sp|Q17060|MRJP3_APIME  Major royal jelly protein 3 precursor...    40   0.003
sp|Q9Y2W1|TR150_HUMAN  Thyroid hormone receptor-associated p...    40   0.003
sp|Q8WXA9|SFR12_HUMAN  Splicing factor, arginine/serine-rich...    39   0.005
sp|P25339|PUF4_YEAST  PUF4 protein                                 39   0.006
sp|Q8BZX4|SFR12_MOUSE  Splicing factor, arginine/serine-rich...    37   0.017
sp|Q5UP41|YR871_MIMIV  Hypothetical protein R871                   37   0.017
sp|Q9NRR4|RNC_HUMAN  Ribonuclease III (RNase III) (Drosha) (...    37   0.017
sp|Q01443|SSP2_PLAYO  Sporozoite surface protein 2 precursor       37   0.023
>sp|Q8CST4|IF2_STAES Translation initiation factor IF-2
 sp|Q5HPS2|IF2_STAEQ Translation initiation factor IF-2
          Length = 720

 Score = 42.0 bits (97), Expect = 7e-04
 Identities = 28/98 (28%), Positives = 49/98 (50%)
 Frame = +3

Query: 177 KKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 356
           K  + K++ S+ K  NK++  +NH  Q SN+   +++++K   K+N   +   KK    K
Sbjct: 44  KALDKKFKASQAKDTNKQNTQNNH--QKSNNKQNSNDKEKQQSKNNS--KPTKKKEQNNK 99

Query: 357 RKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 470
            K   +NN    +Q +NK     K+ +KN     E E+
Sbjct: 100 GKQQNKNNKTNKNQKNNKN----KKNNKNNKPQNEVEE 133
>sp|P38691|KSP1_YEAST Serine/threonine-protein kinase KSP1
          Length = 1029

 Score = 41.6 bits (96), Expect = 0.001
 Identities = 23/87 (26%), Positives = 42/87 (48%), Gaps = 3/87 (3%)
 Frame = +3

Query: 150 RDPYKRRE---PKKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNED 320
           RD +  R+   P   +++Y      +NNK+ R + +Y   +N+ N NH    N+  +   
Sbjct: 516 RDEFNTRDFFTPPSVQHRYMEGFSNNNNKQYRQNRNYNNNNNNSNNNHGSNYNNFNNGNS 575

Query: 321 YERPSKKNSYEKRKPVKQNNYYGDSQM 401
           Y +   KN  + R+P   ++Y G S +
Sbjct: 576 YIKGWNKNFNKYRRP-SSSSYTGKSPL 601

 Score = 30.8 bits (68), Expect = 1.6
 Identities = 21/68 (30%), Positives = 36/68 (52%), Gaps = 2/68 (2%)
 Frame = +3

Query: 183 HENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRK--KNDMKDNEDYERPSKKNSYEK 356
           +  +YR +R   NN  + S+N++   SN+ N+N+     K   K+   Y RPS  +SY  
Sbjct: 542 NNKQYRQNR-NYNNNNNNSNNNH--GSNYNNFNNGNSYIKGWNKNFNKYRRPS-SSSYTG 597

Query: 357 RKPVKQNN 380
           + P+ + N
Sbjct: 598 KSPLSRYN 605
>sp|Q17060|MRJP3_APIME Major royal jelly protein 3 precursor (MRJP-3) (Bee-milk protein)
           (Royal jelly protein RJP57-1)
          Length = 544

 Score = 39.7 bits (91), Expect = 0.003
 Identities = 22/85 (25%), Positives = 43/85 (50%), Gaps = 2/85 (2%)
 Frame = +3

Query: 162 KRREPKKHENKYRTSRYKSNNKRDRSSNHYYQPSN--HGNYNHNRKKNDMKDNEDYERPS 335
           K+   ++++N+   ++   N + D   N   Q  N  +GN  +  K+ND K N + +  +
Sbjct: 454 KQNGNRQNDNRQNDNKQNGNRQNDNKQNGNRQNDNKQNGNRQNGNKQNDNKQNGNRQNDN 513

Query: 336 KKNSYEKRKPVKQNNYYGDSQMDNK 410
           K+N    R+   QNN   +++ DN+
Sbjct: 514 KRNG--NRQNDNQNNQNDNNRNDNQ 536

 Score = 34.7 bits (78), Expect = 0.11
 Identities = 22/88 (25%), Positives = 42/88 (47%)
 Frame = +3

Query: 180 KHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKR 359
           ++ NK   +R   N + D   N        GN  ++ K+N  + N++ +  +++N   K+
Sbjct: 450 QNANKQNGNRQNDNRQNDNKQN--------GNRQNDNKQNGNRQNDNKQNGNRQNG-NKQ 500

Query: 360 KPVKQNNYYGDSQMDNKKPAEIKRQSKN 443
              KQN   G+ Q DNK+    +  ++N
Sbjct: 501 NDNKQN---GNRQNDNKRNGNRQNDNQN 525

 Score = 32.0 bits (71), Expect = 0.73
 Identities = 18/77 (23%), Positives = 40/77 (51%), Gaps = 2/77 (2%)
 Frame = +3

Query: 216 SNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEKRKPVKQ--NNYYG 389
           + N  ++++++      +GN  ++ ++ND K N + +  +K+N   ++   KQ  N   G
Sbjct: 439 NQNANNQNADNQNANKQNGNRQNDNRQNDNKQNGNRQNDNKQNG-NRQNDNKQNGNRQNG 497

Query: 390 DSQMDNKKPAEIKRQSK 440
           + Q DNK+    +  +K
Sbjct: 498 NKQNDNKQNGNRQNDNK 514
>sp|Q9Y2W1|TR150_HUMAN Thyroid hormone receptor-associated protein complex 150 kDa
           component (Trap150) (Thyroid hormone receptor associated
           protein 3)
          Length = 955

 Score = 39.7 bits (91), Expect = 0.003
 Identities = 25/100 (25%), Positives = 41/100 (41%), Gaps = 2/100 (2%)
 Frame = +3

Query: 162 KRREPKKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKN--DMKDNEDYERPS 335
           KRR P      +  +  KS++ R R S+     SNH     +++K+  + K +    RPS
Sbjct: 131 KRRSPSPRSRSHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSAKEKKSSSKDSRPS 190

Query: 336 KKNSYEKRKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKT 455
           +     +   VK+  + G +  D K     K      Y T
Sbjct: 191 QAAGDNQGDEVKEQTFSGGTSQDTKASESSKPWPDATYGT 230
>sp|Q8WXA9|SFR12_HUMAN Splicing factor, arginine/serine-rich 12 (Serine-arginine-rich
           splicing regulatory protein 86) (SRrp86) (Splicing
           regulatory protein 508) (SRrp508)
          Length = 508

 Score = 39.3 bits (90), Expect = 0.005
 Identities = 25/99 (25%), Positives = 46/99 (46%), Gaps = 1/99 (1%)
 Frame = +3

Query: 177 KKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 356
           + H      S  KS+++R RS       S H + +HNR ++  KD    + P KK S  +
Sbjct: 193 RSHTRSKSRSSSKSHSRRKRSQ------SKHRSRSHNRSRSRQKDRRRSKSPHKKRSKSR 246

Query: 357 -RKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 470
            R+  +  ++  D + D ++  + K + K   +  E E+
Sbjct: 247 ERRKSRSRSHSRDKRKDTREKIKEKERVKEKDREKERER 285

 Score = 31.2 bits (69), Expect = 1.2
 Identities = 16/98 (16%), Positives = 46/98 (46%)
 Frame = +3

Query: 177 KKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 356
           K+ ++K+R+  +  +  R +       P    + +  R+K+  + +   +R   +   ++
Sbjct: 211 KRSQSKHRSRSHNRSRSRQKDRRRSKSPHKKRSKSRERRKSRSRSHSRDKRKDTREKIKE 270

Query: 357 RKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 470
           ++ VK+ +   + + + ++  E K + KN  +  E E+
Sbjct: 271 KERVKEKDREKEREREKEREKE-KERGKNKDRDKEREK 307
>sp|P25339|PUF4_YEAST PUF4 protein
          Length = 888

 Score = 38.9 bits (89), Expect = 0.006
 Identities = 20/78 (25%), Positives = 37/78 (47%), Gaps = 4/78 (5%)
 Frame = +3

Query: 183 HENKYRTSRYKSNNKRDRSSN----HYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSY 350
           H +         NNK    SN       + S HG YN+N   N+  +N +    +  NS 
Sbjct: 485 HNDNDNIGNSNYNNKDTGRSNVGKMKNMKNSYHGYYNNNNNNNNNNNNNNNSNATNSNSA 544

Query: 351 EKRKPVKQNNYYGDSQMD 404
           EK++ +++++ + D+ +D
Sbjct: 545 EKQRKIEESSRFADAVLD 562
>sp|Q8BZX4|SFR12_MOUSE Splicing factor, arginine/serine-rich 12 (Serine-arginine-rich
           splicing regulatory protein 86) (SRrp86)
          Length = 494

 Score = 37.4 bits (85), Expect = 0.017
 Identities = 25/99 (25%), Positives = 45/99 (45%), Gaps = 1/99 (1%)
 Frame = +3

Query: 177 KKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 356
           + H      S  KS+++R RS       S H + +HNR ++  KD    + P KK S  +
Sbjct: 196 RSHTRSKSRSSSKSHSRRKRSQ------SKHRSRSHNRSRSRQKDRRRSKSPHKKRSKSR 249

Query: 357 -RKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 470
            R+  +  +   D + D ++  + + + K   K  E E+
Sbjct: 250 ERRKSRSRSRSRDKRKDTREKVKERVKEKEREKEREREK 288

 Score = 30.0 bits (66), Expect = 2.8
 Identities = 18/98 (18%), Positives = 41/98 (41%)
 Frame = +3

Query: 177 KKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYERPSKKNSYEK 356
           K+ ++K+R+  +  +  R +       P    + +  R+K+  +     +R   +   ++
Sbjct: 214 KRSQSKHRSRSHNRSRSRQKDRRRSKSPHKKRSKSRERRKSRSRSRSRDKRKDTREKVKE 273

Query: 357 RKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 470
           R   K+     + + D +K    K + KN  K  E E+
Sbjct: 274 RVKEKEREKEREREKDREKD---KERGKNKDKDREKEK 308
>sp|Q5UP41|YR871_MIMIV Hypothetical protein R871
          Length = 561

 Score = 37.4 bits (85), Expect = 0.017
 Identities = 20/81 (24%), Positives = 39/81 (48%), Gaps = 2/81 (2%)
 Frame = +3

Query: 150 RDPYKRREPKKHEN--KYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDY 323
           + P + R PK+H++  K+++S+   ++K D+SS        H ++    KK D    + Y
Sbjct: 129 KSPSRGRSPKRHKSSKKHKSSKKHKSSKHDKSSKRDKSSKRHKSH----KKKDKSHKKRY 184

Query: 324 ERPSKKNSYEKRKPVKQNNYY 386
             PS   S  + +   ++  Y
Sbjct: 185 RSPSSDRSLSRDRSSSRDRSY 205
>sp|Q9NRR4|RNC_HUMAN Ribonuclease III (RNase III) (Drosha) (p241)
          Length = 1374

 Score = 37.4 bits (85), Expect = 0.017
 Identities = 24/102 (23%), Positives = 49/102 (48%), Gaps = 3/102 (2%)
 Frame = +3

Query: 156 PYKRREPKKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYER-- 329
           P +RR P++ ++ Y   R++ ++   R   H          + +R++ D +   DY+R  
Sbjct: 216 PSERRSPERLKH-YDDHRHRDHS-HGRGERHRSLDRRERGRSPDRRRQDSRYRSDYDRGR 273

Query: 330 -PSKKNSYEKRKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYK 452
            PS+  SYE+ +  ++  +      DN++   ++R  K  YK
Sbjct: 274 TPSRHRSYERSRERERERH---RHRDNRRSPSLERSYKKEYK 312
>sp|Q01443|SSP2_PLAYO Sporozoite surface protein 2 precursor
          Length = 827

 Score = 37.0 bits (84), Expect = 0.023
 Identities = 24/107 (22%), Positives = 40/107 (37%)
 Frame = +3

Query: 150 RDPYKRREPKKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDNEDYER 329
           R+P +R   K   NK   ++   N   + +  +  +PSN    N N   N  K N +   
Sbjct: 395 RNPKRRNPNKPKPNKPNPNKPNPNEPSNPNKPNPNEPSNPNKPNPNEPSNPNKPNPN--E 452

Query: 330 PSKKNSYEKRKPVKQNNYYGDSQMDNKKPAEIKRQSKNPYKTPEPEQ 470
           PS  N     +P+  N     ++  N        +  NP +   P +
Sbjct: 453 PSNPNKPNPNEPLNPNEPSNPNEPSNPNAPSNPNEPSNPNEPSNPNE 499

 Score = 33.5 bits (75), Expect = 0.25
 Identities = 22/69 (31%), Positives = 28/69 (40%), Gaps = 1/69 (1%)
 Frame = +3

Query: 174 PKKHENKYRTSRYKSNNKRDRSSNHYYQPSNHGNYNHNRKKNDMKDN-EDYERPSKKNSY 350
           PKK   K R       NK + +  +  +PSN    N N   N  K N  +   P+K N  
Sbjct: 392 PKKRNPKRRNPNKPKPNKPNPNKPNPNEPSNPNKPNPNEPSNPNKPNPNEPSNPNKPNPN 451

Query: 351 EKRKPVKQN 377
           E   P K N
Sbjct: 452 EPSNPNKPN 460
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,063,313
Number of Sequences: 369166
Number of extensions: 802760
Number of successful extensions: 4604
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3523
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4144
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2733320975
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)