Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_P22
(839 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UKR3|KLK13_HUMAN Kallikrein 13 precursor (Kallikrein-l... 140 6e-33
sp|P80646|CTRB_GADMO Chymotrypsin B [Contains: Chymotrypsin... 127 3e-29
sp|P47796|CTRA_GADMO Chymotrypsin A precursor 126 9e-29
sp|Q9JHJ7|TEST_MOUSE Testisin precursor (Tryptase 4) 124 2e-28
sp|P40313|CTRL_HUMAN Chymotrypsin-like protease CTRL-1 prec... 124 4e-28
sp|Q61955|NRPN_MOUSE Neuropsin precursor (NP) (Kallikrein 8) 123 6e-28
sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma p... 121 2e-27
sp|P00765|TRYP_ASTFL Trypsin I 120 4e-27
sp|O88780|NRPN_RAT Neuropsin precursor (NP) (Kallikrein-8) ... 120 4e-27
sp|Q91VE3|KLK7_MOUSE Kallikrein-7 precursor (Stratum corneu... 118 2e-26
>sp|Q9UKR3|KLK13_HUMAN Kallikrein 13 precursor (Kallikrein-like protein 4) (KLK-L4)
Length = 277
Score = 140 bits (352), Expect = 6e-33
Identities = 88/249 (35%), Positives = 125/249 (50%), Gaps = 1/249 (0%)
Frame = +3
Query: 39 TSGWKIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFK 218
TSG+ + G H P ++ VQG CGG L++ VLTAAHC+ +
Sbjct: 32 TSGF-LPGGYTCFPHSQPWQAALLVQGRLLCGGVLVHPKWVLTAAHCLKE---------- 80
Query: 219 YYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLE-GNDVALIKFDGYISKNQ 395
+KVY+G++ L R+ E++ +V +I HP YR +L +D+ L++ +
Sbjct: 81 --GLKVYLGKHALGRVEAGEQVREVVHSIPHPEYRRSPTHLNHDHDIMLLELQSPVQLT- 137
Query: 396 YDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNV 575
YI L N PG C V GWG+ P + + LQ A I + S+ EC +++
Sbjct: 138 -GYIQTLPLSHNNRLTPGTTCRVSGWGTTTSPQVNYPKTLQCANIQLRSDEECRQVYPG- 195
Query: 576 AYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTR 755
+ +CAG+ SC GDSGGPL C + GIVS+G PCGQP+ P VYTR
Sbjct: 196 KITDNMLCAGTKEGGKDSCEGDSGGPLVC-----NRTLYGIVSWGDFPCGQPDRPGVYTR 250
Query: 756 VSEVAGWIR 782
VS WIR
Sbjct: 251 VSRYVLWIR 259
>sp|P80646|CTRB_GADMO Chymotrypsin B [Contains: Chymotrypsin B chain A; Chymotrypsin B
chain B]
Length = 245
Score = 127 bits (320), Expect = 3e-29
Identities = 89/251 (35%), Positives = 127/251 (50%), Gaps = 3/251 (1%)
Frame = +3
Query: 36 VTSGWKIINGSQAIMHEYPQMVSIKVQ-GNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNT 212
VT +I+NG +A+ H +P VS++ G HFCGG+L+N N V+TAAHC NV T
Sbjct: 10 VTGYARIVNGEEAVPHSWPWQVSLQQSNGFHFCGGSLINENWVVTAAHC-------NVRT 62
Query: 213 FKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKF--DGYIS 386
+ +V +GE+D ++ I + + HP + ND++LIK +
Sbjct: 63 YH----RVIVGEHDKASDENIQ-ILKPSMVFTHPKWDSRTI---NNDISLIKLASPAVLG 114
Query: 387 KNQYDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIF 566
N DV A PG +C GWG T LQ+AA+ ++SN EC + +
Sbjct: 115 TNVSPVCLGESSDVFA---PGMKCVTSGWGLTRYNAPGTPNKLQQAALPLMSNEECSQTW 171
Query: 567 SNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSV 746
N ICAG+ +SC GDSGGPL C + VGIVS+G+ C P+V
Sbjct: 172 GNNMISDVMICAGAA--GATSCMGDSGGPLVCQKDNVWTL-VGIVSWGSSRC-SVTTPAV 227
Query: 747 YTRVSEVAGWI 779
Y RV+E+ GW+
Sbjct: 228 YARVTELRGWV 238
>sp|P47796|CTRA_GADMO Chymotrypsin A precursor
Length = 263
Score = 126 bits (316), Expect = 9e-29
Identities = 82/250 (32%), Positives = 131/250 (52%), Gaps = 2/250 (0%)
Frame = +3
Query: 36 VTSGWKIINGSQAIMHEYPQMVSIKVQ-GNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNT 212
+T +I+NG +A+ H + VS++ Q G HFCGG+L+N N V+TAAHC +++N++
Sbjct: 28 ITGYSRIVNGEEAVPHSWSWQVSLQDQTGFHFCGGSLINENWVVTAAHC--NVKNYH--- 82
Query: 213 FKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKN 392
+V +GE+D ++ V + HP Y + ND+ L+K + N
Sbjct: 83 ------RVVLGEHDRSSNSEGVQVMTVGQVFKHPRYN---GFTINNDILLVKLATPATLN 133
Query: 393 -QYDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFS 569
+ + E D + + G +C GWG DT LQ+AA+ +++N +C + +
Sbjct: 134 MRVSPVCLAETD--DVFEGGMKCVTSGWGLTRYNAADTPALLQQAALPLLTNEQCKKFWG 191
Query: 570 NVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVY 749
N S++ +G SSC GDSGGPL C + VGIVS+G+ C P +P VY
Sbjct: 192 NKI---SDLMICAGAAGASSCMGDSGGPLVCQKAGSWTL-VGIVSWGSGTC-TPTMPGVY 246
Query: 750 TRVSEVAGWI 779
RV+E+ W+
Sbjct: 247 ARVTELRAWV 256
>sp|Q9JHJ7|TEST_MOUSE Testisin precursor (Tryptase 4)
Length = 324
Score = 124 bits (312), Expect = 2e-28
Identities = 90/259 (34%), Positives = 131/259 (50%), Gaps = 14/259 (5%)
Frame = +3
Query: 51 KIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVW---DMRNWNVNTFKY 221
+I+ G A + +P S++V GNH CG TLLN VLTAAHC D +W V
Sbjct: 54 RIVGGDDAELGRWPWQGSLRVWGNHLCGATLLNRRWVLTAAHCFQKDNDPFDWTV----- 108
Query: 222 YQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYD 401
Q L+ + +Q+ + P Y +Y ND+AL+K ++ N +
Sbjct: 109 -QFGELTSRPSLWNLQAYSNRYQIEDIFLSPKYSEQYP----NDIALLKLSSPVTYN--N 161
Query: 402 YIDYNELDVNAISKPGDR--CEVVGWGSMLPPGQDTS----RFLQKAAINIISNSECGRI 563
+I L +N+ K +R C V GWG++ G+D S LQ+ + II+NS C +
Sbjct: 162 FIQPICL-LNSTYKFENRTDCWVTGWGAI---GEDESLPSPNTLQEVQVAIINNSMCNHM 217
Query: 564 FSNVAYVRSNI-----CAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQ 728
+ + R+NI CAG+ +C GDSGGPL C D QVG+VS+G + CG+
Sbjct: 218 YKKPDF-RTNIWGDMVCAGTPEGGKDACFGDSGGPLAC-DQDTVWYQVGVVSWG-IGCGR 274
Query: 729 PNVPSVYTRVSEVAGWIRA 785
PN P VYT +S WI++
Sbjct: 275 PNRPGVYTNISHHYNWIQS 293
>sp|P40313|CTRL_HUMAN Chymotrypsin-like protease CTRL-1 precursor
Length = 264
Score = 124 bits (310), Expect = 4e-28
Identities = 87/252 (34%), Positives = 130/252 (51%), Gaps = 3/252 (1%)
Frame = +3
Query: 33 AVTSGWKIINGSQAIMHEYPQMVSIK-VQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVN 209
A++ +I+NG A++ +P VS++ G HFCGG+L++ + V+TAAHC NV+
Sbjct: 27 ALSFSQRIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWVVTAAHC-------NVS 79
Query: 210 TFKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISK 389
+++ V +GEYD ++ V++ I HPS+ + NDV L+K S
Sbjct: 80 PGRHF---VVLGEYDRSSNAEPLQVLSVSRAITHPSWN---STTMNNDVTLLKL---ASP 130
Query: 390 NQYD--YIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRI 563
QY N G C GWG + G T LQ+ A+ +++ ++C R
Sbjct: 131 AQYTTRISPVCLASSNEALTEGLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQC-RQ 189
Query: 564 FSNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPS 743
+ + S ICAG SSC+GDSGGPL C G + +GIVS+GT C P+
Sbjct: 190 YWGSSITDSMICAGGA--GASSCQGDSGGPLVCQKGNT-WVLIGIVSWGTKNC-NVRAPA 245
Query: 744 VYTRVSEVAGWI 779
VYTRVS+ + WI
Sbjct: 246 VYTRVSKFSTWI 257
>sp|Q61955|NRPN_MOUSE Neuropsin precursor (NP) (Kallikrein 8)
Length = 260
Score = 123 bits (309), Expect = 6e-28
Identities = 79/246 (32%), Positives = 116/246 (47%)
Frame = +3
Query: 45 GWKIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKYY 224
G KI+ G + I H P ++ CGG L+ VLTAAHC K
Sbjct: 30 GSKILEGRECIPHSQPWQAALFQGERLICGGVLVGDRWVLTAAHC------------KKQ 77
Query: 225 QMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYDY 404
+ V +G++ L E+ QV ++I HP Y + +D+ LI+ S N D
Sbjct: 78 KYSVRLGDHSLQSRDQPEQEIQVAQSIQHPCYNNSNPEDHSHDIMLIRLQN--SANLGDK 135
Query: 405 IDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNVAYV 584
+ +L N K G +C + GWG++ P ++ L A + I S ++C R +
Sbjct: 136 VKPVQL-ANLCPKVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERAYPG-KIT 193
Query: 585 RSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSE 764
+CAGS +C+GDSGGPL C DG GI S+G+ PCG+P P VYT++
Sbjct: 194 EGMVCAGSSN-GADTCQGDSGGPLVC-DGMLQ----GITSWGSDPCGKPEKPGVYTKICR 247
Query: 765 VAGWIR 782
WI+
Sbjct: 248 YTTWIK 253
>sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 121 bits (304), Expect = 2e-27
Identities = 85/256 (33%), Positives = 126/256 (49%), Gaps = 6/256 (2%)
Frame = +3
Query: 30 CAVTSGWKIINGSQAIMHEYPQMVSIKVQ---GNHFCGGTLLNGNTVLTAAHCVWDMRNW 200
C +I+ G+ A + E+P VS++V+ H CGG+++ VLTAAHC +
Sbjct: 383 CTTKINARIVGGTNASLGEWPWQVSLQVKLVSQTHLCGGSIIGRQWVLTAAHCFDGIPYP 442
Query: 201 NVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGN-DVALIKFDG 377
+V ++Y G L + ++ + IIH EY EGN D+ALIK
Sbjct: 443 DV-------WRIYGGILSLSEITKETPSSRIKELIIH----QEYKVSEGNYDIALIKLQT 491
Query: 378 YISKNQYD--YIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSE 551
++ ++ ++ D N I C V GWG G+ T LQKA I ++ N E
Sbjct: 492 PLNYTEFQKPICLPSKADTNTIYT---NCWVTGWGYTKEQGE-TQNILQKATIPLVPNEE 547
Query: 552 CGRIFSNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQP 731
C + + + + ICAG T +C+GDSGGPL C + + VGI S+G CG+
Sbjct: 548 CQKKYRDYVINKQMICAGYKEGGTDACKGDSGGPLVCKHSGRWQL-VGITSWGE-GCGRK 605
Query: 732 NVPSVYTRVSEVAGWI 779
+ P VYT+VSE WI
Sbjct: 606 DQPGVYTKVSEYMDWI 621
>sp|P00765|TRYP_ASTFL Trypsin I
Length = 237
Score = 120 bits (302), Expect = 4e-27
Identities = 82/254 (32%), Positives = 127/254 (50%), Gaps = 8/254 (3%)
Frame = +3
Query: 54 IINGSQAIMHEYPQMVSIKVQ----GNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKY 221
I+ G+ A++ E+P +S + HFCG ++ N N +TA HCV+ N +
Sbjct: 1 IVGGTDAVLGEFPYQLSFQETFLGFSFHFCGASIYNENYAITAGHCVYGDDYENPSG--- 57
Query: 222 YQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYD 401
+++ GE D+ G E+ V+K I+H ++ ++ L ND++L+K G ++ N
Sbjct: 58 --LQIVAGELDMSVNEGSEQTITVSKIILHENFDYD---LLDNDISLLKLSGSLTFN--- 109
Query: 402 YIDYNELDVNAISKPGDRCE----VVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFS 569
N + A+ G V GWG+ G +T LQK + ++S++EC +
Sbjct: 110 ----NNVAPIALPAQGHTATGNVIVTGWGTT-SEGGNTPDVLQKVTVPLVSDAECRDDYG 164
Query: 570 NVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVY 749
S ICAG SC+GDSGGPL +D ++ GIVS+G C +P P VY
Sbjct: 165 ADEIFDSMICAGVPEGGKDSCQGDSGGPLAASDTGSTYL-AGIVSWG-YGCARPGYPGVY 222
Query: 750 TRVSEVAGWIRANA 791
T VS WI+ANA
Sbjct: 223 TEVSYHVDWIKANA 236
>sp|O88780|NRPN_RAT Neuropsin precursor (NP) (Kallikrein-8) (Brain serine protease 1)
Length = 260
Score = 120 bits (302), Expect = 4e-27
Identities = 77/246 (31%), Positives = 115/246 (46%)
Frame = +3
Query: 45 GWKIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKYY 224
G KI+ G + H P ++ CGG L+ VLTAAHC D KY
Sbjct: 30 GSKILEGQECKPHSQPWQTALFQGERLVCGGVLVGDRWVLTAAHCKKD---------KY- 79
Query: 225 QMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYDY 404
V +G++ L + E+ QV ++I HP + +D+ LI+ S N D
Sbjct: 80 --SVRLGDHSLQKRDEPEQEIQVARSIQHPCFNSSNPEDHSHDIMLIRLQN--SANLGDK 135
Query: 405 IDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNVAYV 584
+ EL N K G +C + GWG++ P ++ L A + I S ++C R +
Sbjct: 136 VKPIEL-ANLCPKVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERAYPG-KIT 193
Query: 585 RSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSE 764
+CAGS +C+GDSGGPL C + GI ++G+ PCG+P P VYT++
Sbjct: 194 EGMVCAGSSN-GADTCQGDSGGPLVCNG-----VLQGITTWGSDPCGKPEKPGVYTKICR 247
Query: 765 VAGWIR 782
WI+
Sbjct: 248 YTNWIK 253
>sp|Q91VE3|KLK7_MOUSE Kallikrein-7 precursor (Stratum corneum chymotryptic enzyme)
(Thymopsin)
Length = 249
Score = 118 bits (296), Expect = 2e-26
Identities = 82/261 (31%), Positives = 130/261 (49%), Gaps = 2/261 (0%)
Frame = +3
Query: 3 SFLFVLITYCAVTSGW--KIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAH 176
S + VL++ T+G +II+G + +P V++ CGG L++ VLTAAH
Sbjct: 7 SLITVLLSLALETAGQGERIIDGYKCKEGSHPWQVALLKGNQLHCGGVLVDKYWVLTAAH 66
Query: 177 CVWDMRNWNVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDV 356
C K Q +V +G D +KI + TK+ HP Y + ND+
Sbjct: 67 C------------KMGQYQVQLGS-DKIGDQSAQKI-KATKSFRHPGYSTK---THVNDI 109
Query: 357 ALIKFDGYISKNQYDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINI 536
L++ D + + ++ +L + PG C V GWG+ P L + + +
Sbjct: 110 MLVRLDEPVKMSSK--VEAVQLPEHC-EPPGTSCTVSGWGTTTSPDVTFPSDLMCSDVKL 166
Query: 537 ISNSECGRIFSNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTV 716
IS+ EC +++ ++ ++ +CAG T++C GDSGGPL C D + G+VS+GT
Sbjct: 167 ISSRECKKVYKDLLG-KTMLCAGIPDSKTNTCNGDSGGPLVCNDTLQ-----GLVSWGTY 220
Query: 717 PCGQPNVPSVYTRVSEVAGWI 779
PCGQPN P VYT+V + W+
Sbjct: 221 PCGQPNDPGVYTQVCKYKRWV 241
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105,092,088
Number of Sequences: 369166
Number of extensions: 2329760
Number of successful extensions: 7216
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5878
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6115
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8197207050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)