Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_O19
(628 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P11141|HSP7F_CAEEL Heat shock 70 kDa protein F, mitochon... 165 1e-40
sp|P38646|GRP75_HUMAN Stress-70 protein, mitochondrial prec... 164 2e-40
sp|P38647|GRP75_MOUSE Stress-70 protein, mitochondrial prec... 164 2e-40
sp|O35501|GRP75_CRIGR Stress-70 protein, mitochondrial prec... 164 2e-40
sp|P48721|GRP75_RAT Stress-70 protein, mitochondrial precur... 163 4e-40
sp|P39987|HSP7E_YEAST Heat shock protein SSC3, mitochondria... 149 6e-36
sp|P29845|HSP7E_DROME Heat shock 70 kDa protein cognate 5 147 3e-35
sp|P12398|HSP77_YEAST Heat shock protein SSC1, mitochondria... 146 5e-35
sp|P22774|HSP7M_SCHPO Heat shock 70 kDa protein, mitochondr... 141 2e-33
sp|Q01899|HSP7M_PHAVU Heat shock 70 kDa protein, mitochondr... 140 3e-33
>sp|P11141|HSP7F_CAEEL Heat shock 70 kDa protein F, mitochondrial precursor
Length = 657
Score = 165 bits (417), Expect = 1e-40
Identities = 94/147 (63%), Positives = 108/147 (73%)
Frame = +3
Query: 3 TPSIVAFTGDGERLVWNTR*ASSCY*FS*HTSCYQKINLGEGLMNLKFRKI*KMYLIKFV 182
TPS VAFT DGERLV + S +T K +G + + +K K+ K V
Sbjct: 65 TPSTVAFTADGERLVGAPAKRQAVT-NSANTLFATKRLIGRRYEDPEVQKDLKVVPYKIV 123
Query: 183 RASNGDAWVEAQKKMYFPSQIGAFVLMKMKETAENFLGTSVKNAVITVPAYFNDSQRQAT 362
+ASNGDAWVEAQ K+Y PSQ+GAFVLMKMKETAE++LGT+V NAV+TVPAYFNDSQRQAT
Sbjct: 124 KASNGDAWVEAQGKVYSPSQVGAFVLMKMKETAESYLGTTVNNAVVTVPAYFNDSQRQAT 183
Query: 363 KDAGQISGLNVLRVINEPTAAAFGIWL 443
KDAGQISGLNVLRVINEPTAAA L
Sbjct: 184 KDAGQISGLNVLRVINEPTAAALAYGL 210
Score = 38.1 bits (87), Expect = 0.018
Identities = 47/161 (29%), Positives = 67/161 (41%), Gaps = 4/161 (2%)
Frame = +2
Query: 119 GRRFDEPEVQKDMKNVSYKICKSVE---W*CMGRSSKENVFSKSNRG-FCINENERNGRK 286
GRR+++PEVQKD+K V YKI K+ W ++ V+S S G F + + +
Sbjct: 103 GRRYEDPEVQKDLKVVPYKIVKASNGDAW----VEAQGKVYSPSQVGAFVLMKMKETAES 158
Query: 287 FFRNFC*KCSYHCASLF**FSKTSYQRCGSNIRFECLKSYQ*TNCCCFWHMA*IKLMIK* 466
+ + F + + + G L+ + K
Sbjct: 159 YLGTTVNNAVVTVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKDAGDKI 218
Query: 467 FAVF*LWVVEHFDVSVLEIQKGVFES*IN*W*YISLGGEDF 589
AV+ L FDVS+LEIQKGVFE LGGEDF
Sbjct: 219 IAVYDLGGGT-FDVSILEIQKGVFEVKSTNG-DTFLGGEDF 257
>sp|P38646|GRP75_HUMAN Stress-70 protein, mitochondrial precursor (75 kDa glucose
regulated protein) (GRP 75) (Peptide-binding protein 74)
(PBP74) (Mortalin) (MOT)
Length = 679
Score = 164 bits (414), Expect = 2e-40
Identities = 95/147 (64%), Positives = 106/147 (72%)
Frame = +3
Query: 3 TPSIVAFTGDGERLVWNTR*ASSCY*FS*HTSCYQKINLGEGLMNLKFRKI*KMYLIKFV 182
TPS+VAFT DGERLV + + +T K +G + + +K K K V
Sbjct: 87 TPSVVAFTADGERLVGMPAKRQAVTNPN-NTFYATKRLIGRRYDDPEVQKDIKNVPFKIV 145
Query: 183 RASNGDAWVEAQKKMYFPSQIGAFVLMKMKETAENFLGTSVKNAVITVPAYFNDSQRQAT 362
RASNGDAWVEA K+Y PSQIGAFVLMKMKETAEN+LG + KNAVITVPAYFNDSQRQAT
Sbjct: 146 RASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQAT 205
Query: 363 KDAGQISGLNVLRVINEPTAAAFGIWL 443
KDAGQISGLNVLRVINEPTAAA L
Sbjct: 206 KDAGQISGLNVLRVINEPTAAALAYGL 232
Score = 31.2 bits (69), Expect = 2.3
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +1
Query: 433 AYGLNKTDDKIICSFLTLGGGTF 501
AYGL+K++DK+I + LGGGTF
Sbjct: 229 AYGLDKSEDKVIAVY-DLGGGTF 250
Score = 30.8 bits (68), Expect = 3.0
Identities = 19/32 (59%), Positives = 21/32 (65%)
Frame = +2
Query: 500 FDVSVLEIQKGVFES*IN*W*YISLGGEDF*Q 595
FD+S+LEIQKGVFE LGGEDF Q
Sbjct: 250 FDISILEIQKGVFEVKSTNG-DTFLGGEDFDQ 280
>sp|P38647|GRP75_MOUSE Stress-70 protein, mitochondrial precursor (75 kDa glucose
regulated protein) (GRP 75) (Peptide-binding protein 74)
(PBP74) (P66 MOT) (Mortalin)
Length = 679
Score = 164 bits (414), Expect = 2e-40
Identities = 95/147 (64%), Positives = 106/147 (72%)
Frame = +3
Query: 3 TPSIVAFTGDGERLVWNTR*ASSCY*FS*HTSCYQKINLGEGLMNLKFRKI*KMYLIKFV 182
TPS+VAFT DGERLV + + +T K +G + + +K K K V
Sbjct: 87 TPSVVAFTADGERLVGMPAKRQAVTNPN-NTFYATKRLIGRRYDDPEVQKDTKNVPFKIV 145
Query: 183 RASNGDAWVEAQKKMYFPSQIGAFVLMKMKETAENFLGTSVKNAVITVPAYFNDSQRQAT 362
RASNGDAWVEA K+Y PSQIGAFVLMKMKETAEN+LG + KNAVITVPAYFNDSQRQAT
Sbjct: 146 RASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQAT 205
Query: 363 KDAGQISGLNVLRVINEPTAAAFGIWL 443
KDAGQISGLNVLRVINEPTAAA L
Sbjct: 206 KDAGQISGLNVLRVINEPTAAALAYGL 232
Score = 31.2 bits (69), Expect = 2.3
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +1
Query: 433 AYGLNKTDDKIICSFLTLGGGTF 501
AYGL+K++DK+I + LGGGTF
Sbjct: 229 AYGLDKSEDKVIAVY-DLGGGTF 250
Score = 30.8 bits (68), Expect = 3.0
Identities = 19/32 (59%), Positives = 21/32 (65%)
Frame = +2
Query: 500 FDVSVLEIQKGVFES*IN*W*YISLGGEDF*Q 595
FD+S+LEIQKGVFE LGGEDF Q
Sbjct: 250 FDISILEIQKGVFEVKSTNG-DTFLGGEDFDQ 280
>sp|O35501|GRP75_CRIGR Stress-70 protein, mitochondrial precursor (75 kDa glucose
regulated protein) (GRP 75)
Length = 679
Score = 164 bits (414), Expect = 2e-40
Identities = 95/147 (64%), Positives = 106/147 (72%)
Frame = +3
Query: 3 TPSIVAFTGDGERLVWNTR*ASSCY*FS*HTSCYQKINLGEGLMNLKFRKI*KMYLIKFV 182
TPS+VAFT DGERLV + + +T K +G + + +K K K V
Sbjct: 87 TPSVVAFTADGERLVGMPAKRQAVTNPN-NTFYATKRLIGRRYDDPEVQKDTKNVPFKIV 145
Query: 183 RASNGDAWVEAQKKMYFPSQIGAFVLMKMKETAENFLGTSVKNAVITVPAYFNDSQRQAT 362
RASNGDAWVEA K+Y PSQIGAFVLMKMKETAEN+LG + KNAVITVPAYFNDSQRQAT
Sbjct: 146 RASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQAT 205
Query: 363 KDAGQISGLNVLRVINEPTAAAFGIWL 443
KDAGQISGLNVLRVINEPTAAA L
Sbjct: 206 KDAGQISGLNVLRVINEPTAAALAYGL 232
Score = 31.6 bits (70), Expect = 1.7
Identities = 15/23 (65%), Positives = 19/23 (82%)
Frame = +1
Query: 433 AYGLNKTDDKIICSFLTLGGGTF 501
AYGL+K++DKII + LGGGTF
Sbjct: 229 AYGLDKSEDKIIAVY-DLGGGTF 250
Score = 30.8 bits (68), Expect = 3.0
Identities = 19/32 (59%), Positives = 21/32 (65%)
Frame = +2
Query: 500 FDVSVLEIQKGVFES*IN*W*YISLGGEDF*Q 595
FD+S+LEIQKGVFE LGGEDF Q
Sbjct: 250 FDISILEIQKGVFEVKSTNG-DTFLGGEDFDQ 280
>sp|P48721|GRP75_RAT Stress-70 protein, mitochondrial precursor (75 kDa glucose
regulated protein) (GRP 75) (Peptide-binding protein 74)
(PBP74) (MTHSP70) (Mortalin)
Length = 679
Score = 163 bits (412), Expect = 4e-40
Identities = 95/147 (64%), Positives = 106/147 (72%)
Frame = +3
Query: 3 TPSIVAFTGDGERLVWNTR*ASSCY*FS*HTSCYQKINLGEGLMNLKFRKI*KMYLIKFV 182
TPS+VAFT DGERLV + + +T K +G + + +K K K V
Sbjct: 87 TPSVVAFTPDGERLVGMPAKRQAVTNPN-NTFYATKRLIGRRYDDPEVQKDTKNVPFKIV 145
Query: 183 RASNGDAWVEAQKKMYFPSQIGAFVLMKMKETAENFLGTSVKNAVITVPAYFNDSQRQAT 362
RASNGDAWVEA K+Y PSQIGAFVLMKMKETAEN+LG + KNAVITVPAYFNDSQRQAT
Sbjct: 146 RASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQAT 205
Query: 363 KDAGQISGLNVLRVINEPTAAAFGIWL 443
KDAGQISGLNVLRVINEPTAAA L
Sbjct: 206 KDAGQISGLNVLRVINEPTAAALAYGL 232
Score = 31.2 bits (69), Expect = 2.3
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +1
Query: 433 AYGLNKTDDKIICSFLTLGGGTF 501
AYGL+K++DK+I + LGGGTF
Sbjct: 229 AYGLDKSEDKVIAVY-DLGGGTF 250
Score = 30.8 bits (68), Expect = 3.0
Identities = 19/32 (59%), Positives = 21/32 (65%)
Frame = +2
Query: 500 FDVSVLEIQKGVFES*IN*W*YISLGGEDF*Q 595
FD+S+LEIQKGVFE LGGEDF Q
Sbjct: 250 FDISILEIQKGVFEVKSTNG-DTFLGGEDFDQ 280
>sp|P39987|HSP7E_YEAST Heat shock protein SSC3, mitochondrial precursor (Extracellular
matrix protein 10)
Length = 644
Score = 149 bits (376), Expect = 6e-36
Identities = 84/147 (57%), Positives = 100/147 (68%)
Frame = +3
Query: 3 TPSIVAFTGDGERLVWNTR*ASSCY*FS*HTSCYQKINLGEGLMNLKFRKI*KMYLIKFV 182
TPS+VAFT DGERLV S S +T K +G + + ++ K V
Sbjct: 61 TPSVVAFTKDGERLVGEPAKRQSVI-NSENTLFATKRLIGRRFEDAEVQRDINQVPFKIV 119
Query: 183 RASNGDAWVEAQKKMYFPSQIGAFVLMKMKETAENFLGTSVKNAVITVPAYFNDSQRQAT 362
+ SNGDAWVEA+ + Y P+QIG F+L KMKETAE +L SVKNAV+TVPAYFND+QRQAT
Sbjct: 120 KHSNGDAWVEARNRTYSPAQIGGFILNKMKETAEAYLAKSVKNAVVTVPAYFNDAQRQAT 179
Query: 363 KDAGQISGLNVLRVINEPTAAAFGIWL 443
KDAGQI GLNVLRV+NEPTAAA L
Sbjct: 180 KDAGQIIGLNVLRVVNEPTAAALAYGL 206
Score = 29.6 bits (65), Expect = 6.6
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +1
Query: 433 AYGLNKTDDKIICSFLTLGGGTF 501
AYGL+K++ K+I F LGGGTF
Sbjct: 203 AYGLDKSEPKVIAVF-DLGGGTF 224
>sp|P29845|HSP7E_DROME Heat shock 70 kDa protein cognate 5
Length = 686
Score = 147 bits (370), Expect = 3e-35
Identities = 88/143 (61%), Positives = 101/143 (70%), Gaps = 1/143 (0%)
Frame = +3
Query: 3 TPSIVAFTGDGERLVWNTR*ASSCY*FS*HTSCYQKINLGEGLMNLKFRKI*KMYLIKFV 182
TPS VAFT DGERLV + S +T K +G + + +K K V
Sbjct: 86 TPSHVAFTKDGERLVGMPAKRQAVT-NSANTFYATKRLIGRRFDDPEVKKDITNLSYKVV 144
Query: 183 RASNGDAWVEAQK-KMYFPSQIGAFVLMKMKETAENFLGTSVKNAVITVPAYFNDSQRQA 359
+ASNGDAWV + K+Y PSQIGAF+LMKMKETAE +L T VKNAV+TVPAYFNDSQRQA
Sbjct: 145 KASNGDAWVSSTDGKVYSPSQIGAFILMKMKETAEAYLNTPVKNAVVTVPAYFNDSQRQA 204
Query: 360 TKDAGQISGLNVLRVINEPTAAA 428
TKDAGQI+GLNVLRVINEPTAAA
Sbjct: 205 TKDAGQIAGLNVLRVINEPTAAA 227
Score = 46.2 bits (108), Expect = 7e-05
Identities = 51/167 (30%), Positives = 74/167 (44%), Gaps = 2/167 (1%)
Frame = +2
Query: 95 FMLPKD*FGRRFDEPEVQKDMKNVSYKICKSVEW*CMGRSSKENVFSKSNRG-FCINENE 271
F K GRRFD+PEV+KD+ N+SYK+ K+ S+ V+S S G F + + +
Sbjct: 116 FYATKRLIGRRFDDPEVKKDITNLSYKVVKASNGDAWVSSTDGKVYSPSQIGAFILMKMK 175
Query: 272 RNGRKFFRNFC*KCSYHCASLF**FSKTSYQRCGSNIRFECLKSY-Q*TNCCCFWHMA*I 448
+ + F + + + G L+ + T + M
Sbjct: 176 ETAEAYLNTPVKNAVVTVPAYFNDSQRQATKDAGQIAGLNVLRVINEPTAAALAYGMD-- 233
Query: 449 KLMIK*FAVF*LWVVEHFDVSVLEIQKGVFES*IN*W*YISLGGEDF 589
K K AV+ L FD+S+LEIQKGVFE + LGGEDF
Sbjct: 234 KTEDKIIAVYDLGGGT-FDISILEIQKGVFEVKSTNGDTL-LGGEDF 278
Score = 32.3 bits (72), Expect = 1.0
Identities = 15/23 (65%), Positives = 19/23 (82%)
Frame = +1
Query: 433 AYGLNKTDDKIICSFLTLGGGTF 501
AYG++KT+DKII + LGGGTF
Sbjct: 229 AYGMDKTEDKIIAVY-DLGGGTF 250
>sp|P12398|HSP77_YEAST Heat shock protein SSC1, mitochondrial precursor (Endonuclease SCEI
75 kDa subunit)
Length = 654
Score = 146 bits (368), Expect = 5e-35
Identities = 84/148 (56%), Positives = 101/148 (68%)
Frame = +3
Query: 3 TPSIVAFTGDGERLVWNTR*ASSCY*FS*HTSCYQKINLGEGLMNLKFRKI*KMYLIKFV 182
TPS+VAFT +GERLV + +T K +G + + ++ K K V
Sbjct: 64 TPSVVAFTKEGERLVGIPAKRQAVVNPE-NTLFATKRLIGRRFEDAEVQRDIKQVPYKIV 122
Query: 183 RASNGDAWVEAQKKMYFPSQIGAFVLMKMKETAENFLGTSVKNAVITVPAYFNDSQRQAT 362
+ SNGDAWVEA+ + Y P+QIG FVL KMKETAE +LG VKNAV+TVPAYFNDSQRQAT
Sbjct: 123 KHSNGDAWVEARGQTYSPAQIGGFVLNKMKETAEAYLGKPVKNAVVTVPAYFNDSQRQAT 182
Query: 363 KDAGQISGLNVLRVINEPTAAAFGIWLK 446
KDAGQI GLNVLRV+NEPTAAA L+
Sbjct: 183 KDAGQIVGLNVLRVVNEPTAAALAYGLE 210
Score = 30.8 bits (68), Expect = 3.0
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +1
Query: 433 AYGLNKTDDKIICSFLTLGGGTF 501
AYGL K+D K++ F LGGGTF
Sbjct: 206 AYGLEKSDSKVVAVF-DLGGGTF 227
>sp|P22774|HSP7M_SCHPO Heat shock 70 kDa protein, mitochondrial precursor
Length = 674
Score = 141 bits (355), Expect = 2e-33
Identities = 81/147 (55%), Positives = 97/147 (65%)
Frame = +3
Query: 3 TPSIVAFTGDGERLVWNTR*ASSCY*FS*HTSCYQKINLGEGLMNLKFRKI*KMYLIKFV 182
TPS+VAFT DGERLV + + +T K +G + ++ K K V
Sbjct: 84 TPSVVAFTKDGERLVGVSAKRQAVINPE-NTFFATKRLIGRRFKEPEVQRDIKEVPYKIV 142
Query: 183 RASNGDAWVEAQKKMYFPSQIGAFVLMKMKETAENFLGTSVKNAVITVPAYFNDSQRQAT 362
SNGDAW+EA+ K Y PSQIG F+L KM+ETA +LG VKNAV+TVPAYFNDSQRQAT
Sbjct: 143 EHSNGDAWLEARGKTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQAT 202
Query: 363 KDAGQISGLNVLRVINEPTAAAFGIWL 443
K AG I+GLNVLRV+NEPTAAA L
Sbjct: 203 KAAGAIAGLNVLRVVNEPTAAALAYGL 229
>sp|Q01899|HSP7M_PHAVU Heat shock 70 kDa protein, mitochondrial precursor
Length = 675
Score = 140 bits (353), Expect = 3e-33
Identities = 97/205 (47%), Positives = 116/205 (56%), Gaps = 4/205 (1%)
Frame = +3
Query: 3 TPSIVAFTGDGERLVWNTR*ASSCY*FS*HTSCYQKINLGEGLMNLKFRKI*KMYLIKFV 182
TPS+VAF GE LV T +T K +G + + +K KM K V
Sbjct: 85 TPSVVAFNQKGELLV-GTPAKRQAVTNPTNTVFGTKRLIGRRFDDPQTQKEMKMVPFKIV 143
Query: 183 RASNGDAWVEAQKKMYFPSQIGAFVLMKMKETAENFLGTSVKNAVITVPAYFNDSQRQAT 362
+A NGDAWVEA + Y PSQIGAFVL KMKETAE +LG SV AVITVPAYFND+QRQAT
Sbjct: 144 KAPNGDAWVEANGQQYSPSQIGAFVLTKMKETAEAYLGKSVSKAVITVPAYFNDAQRQAT 203
Query: 363 KDAGQISGLNVLRVINEPTAAAFGIWLK*N***NN----LQFFDFGWWNILMXXXXXXXX 530
KDAG+I+GL+V R+INEPTAAA + NN + FD G
Sbjct: 204 KDAGRIAGLDVQRIINEPTAAALSYGM------NNKEGLIAVFDLG-GGTFDVSILEISN 256
Query: 531 XXXXXXXTSGDTFLWVVKTFDNVLI 605
T+GDTFL + FDN L+
Sbjct: 257 GVFEVKATNGDTFLG-GEDFDNALL 280
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,751,184
Number of Sequences: 369166
Number of extensions: 1316447
Number of successful extensions: 4102
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2967
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3886
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4974853140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)