Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_O16
(436 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9VPH7|ERF1_DROME Eukaryotic peptide chain release facto... 78 9e-15
sp|O59948|ERF1_PODAN Eukaryotic peptide chain release facto... 77 2e-14
sp|P35615|ERF1_XENLA Eukaryotic peptide chain release facto... 76 4e-14
sp|Q8BWY3|ERF1_MOUSE Eukaryotic peptide chain release facto... 74 1e-13
sp|P62498|ERF1_XENTR Eukaryotic peptide chain release facto... 74 1e-13
sp|Q5R4C7|ERF1_PONPY Eukaryotic peptide chain release facto... 74 1e-13
sp|Q9GR88|ERF1_POLMI Eukaryotic peptide chain release facto... 72 7e-13
sp|P12385|ERF1_YEAST Eukaryotic peptide chain release facto... 70 2e-12
sp|Q9NCP1|ERF1_GIALA Eukaryotic peptide chain release facto... 69 3e-12
sp|Q5CD97|ERF1_DIDNA Eukaryotic peptide chain release facto... 69 4e-12
>sp|Q9VPH7|ERF1_DROME Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1)
Length = 438
Score = 77.8 bits (190), Expect = 9e-15
Identities = 39/62 (62%), Positives = 47/62 (75%)
Frame = +3
Query: 18 QLFLDFLTLKYKDYGAILEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQDMGNDILNDQE 197
Q L++L YK +GA LEI++DKS EGSQFV+GFGGIGGILRYKVDFQ M D L++
Sbjct: 373 QPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLDELDNDG 432
Query: 198 ED 203
D
Sbjct: 433 FD 434
>sp|O59948|ERF1_PODAN Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1)
Length = 435
Score = 77.0 bits (188), Expect = 2e-14
Identities = 36/64 (56%), Positives = 49/64 (76%)
Frame = +3
Query: 15 EQLFLDFLTLKYKDYGAILEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQDMGNDILNDQ 194
++ FL+++ YKD+G LE VSD+S EG+QFVKGFGGIGGILRYKV+F+ LN+
Sbjct: 372 QESFLEWIAEHYKDFGTTLEFVSDRSTEGNQFVKGFGGIGGILRYKVNFEQ-----LNEV 426
Query: 195 EEDD 206
++DD
Sbjct: 427 DDDD 430
>sp|P35615|ERF1_XENLA Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1) (Omnipotent suppressor
protein 1 homolog) (SUP1 homolog)
Length = 437
Score = 75.9 bits (185), Expect = 4e-14
Identities = 38/63 (60%), Positives = 48/63 (76%), Gaps = 2/63 (3%)
Frame = +3
Query: 27 LDFLTLKYKDYGAILEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQDMGNDILNDQ--EE 200
L++ YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQ M ++D+ +
Sbjct: 375 LEWFANSYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMDYQGVDDEFFDL 434
Query: 201 DDF 209
DD+
Sbjct: 435 DDY 437
>sp|Q8BWY3|ERF1_MOUSE Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1)
sp|P62497|ERF1_RABIT Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1)
sp|P62496|ERF1_MESAU Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1) (Cl1 protein)
sp|P62495|ERF1_HUMAN Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1) (TB3-1) (Cl1 protein)
sp|Q5U2Q7|ERF1_RAT Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1)
Length = 437
Score = 74.3 bits (181), Expect = 1e-13
Identities = 35/48 (72%), Positives = 40/48 (83%)
Frame = +3
Query: 27 LDFLTLKYKDYGAILEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQDM 170
L++ YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQ M
Sbjct: 375 LEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGM 422
>sp|P62498|ERF1_XENTR Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1)
Length = 437
Score = 74.3 bits (181), Expect = 1e-13
Identities = 35/48 (72%), Positives = 40/48 (83%)
Frame = +3
Query: 27 LDFLTLKYKDYGAILEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQDM 170
L++ YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQ M
Sbjct: 375 LEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGM 422
>sp|Q5R4C7|ERF1_PONPY Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1)
Length = 437
Score = 74.3 bits (181), Expect = 1e-13
Identities = 35/48 (72%), Positives = 40/48 (83%)
Frame = +3
Query: 27 LDFLTLKYKDYGAILEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQDM 170
L++ YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQ M
Sbjct: 375 LEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGM 422
>sp|Q9GR88|ERF1_POLMI Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1)
Length = 435
Score = 71.6 bits (174), Expect = 7e-13
Identities = 34/59 (57%), Positives = 44/59 (74%)
Frame = +3
Query: 27 LDFLTLKYKDYGAILEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQDMGNDILNDQEED 203
L++ YK +G+ LEIV+D+S EGSQFVKGFGGIGG+LRY++DFQ M ND + D
Sbjct: 375 LEWFANNYKKFGSTLEIVTDRSQEGSQFVKGFGGIGGMLRYRIDFQGME---FNDDDPD 430
>sp|P12385|ERF1_YEAST Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1) (Omnipotent suppressor
protein 1)
Length = 437
Score = 70.1 bits (170), Expect = 2e-12
Identities = 32/65 (49%), Positives = 48/65 (73%)
Frame = +3
Query: 15 EQLFLDFLTLKYKDYGAILEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQDMGNDILNDQ 194
E+ +++L YK++GA LE ++DKS+EG+QFV GFGGIG +LRYKV+F+ L D+
Sbjct: 366 EEPLIEWLAANYKNFGATLEFITDKSSEGAQFVTGFGGIGAMLRYKVNFEQ-----LVDE 420
Query: 195 EEDDF 209
ED++
Sbjct: 421 SEDEY 425
>sp|Q9NCP1|ERF1_GIALA Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1)
Length = 457
Score = 69.3 bits (168), Expect = 3e-12
Identities = 31/66 (46%), Positives = 47/66 (71%)
Frame = +3
Query: 12 GEQLFLDFLTLKYKDYGAILEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQDMGNDILND 191
G+QL L++ YK+YGA LE ++++SAEG+QF GFGGIGG+LR++VD + + +
Sbjct: 385 GDQLLLEWFVDNYKNYGASLEFITNRSAEGTQFCSGFGGIGGLLRWQVDLVEAAR-FMQE 443
Query: 192 QEEDDF 209
+ED F
Sbjct: 444 SDEDSF 449
>sp|Q5CD97|ERF1_DIDNA Eukaryotic peptide chain release factor subunit 1 (eRF1)
(Eukaryotic release factor 1)
Length = 437
Score = 68.9 bits (167), Expect = 4e-12
Identities = 36/56 (64%), Positives = 44/56 (78%), Gaps = 2/56 (3%)
Frame = +3
Query: 48 YKDYGAILEIVSDKSAEGSQFVKGFGGIGGILRYKV--DFQDMGNDILNDQEEDDF 209
YK Y + LEIV+DKS+EGSQF+KGFGGIGGILRYK+ DF D N+ N+ +DDF
Sbjct: 383 YKKYVSHLEIVTDKSSEGSQFLKGFGGIGGILRYKMDTDFDDTENN--NEWNDDDF 436
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,218,267
Number of Sequences: 369166
Number of extensions: 586926
Number of successful extensions: 1339
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1316
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1339
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2194785120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)