Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_O04
(726 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P29052|TF2B_DROME Transcription initiation factor IIB (G... 112 1e-24
sp|Q9NHP7|TF2B_DROVI Transcription initiation factor IIB (G... 112 1e-24
sp|O16991|TF2B_CAEEL Transcription initiation factor IIB (G... 105 1e-22
sp|P62916|TF2B_RAT Transcription initiation factor IIB (Gen... 102 8e-22
sp|Q00403|TF2B_HUMAN Transcription initiation factor IIB (G... 101 2e-21
sp|P29054|TF2B_XENLA Transcription initiation factor IIB (G... 100 3e-21
sp|O13749|TF2B_SCHPO Transcription initiation factor IIB (G... 78 3e-14
sp|Q05959|TF2B_KLULA Transcription initiation factor IIB (G... 74 3e-13
sp|O28970|TF2B_ARCFU Transcription initiation factor IIB (T... 67 4e-11
sp|P29055|TF2B_YEAST Transcription initiation factor IIB (G... 67 5e-11
>sp|P29052|TF2B_DROME Transcription initiation factor IIB (General transcription factor
TFIIB)
Length = 315
Score = 112 bits (279), Expect = 1e-24
Identities = 59/127 (46%), Positives = 85/127 (66%), Gaps = 1/127 (0%)
Frame = +3
Query: 144 KRILEKFSKKFVHILSTSVGNATGEDFMARFCYQLGIPKL-EKIAKEISKKALDDNMVAG 320
K+ + + K + L TSV T DFM RFC L +P + ++ A I+KKA++ ++V G
Sbjct: 187 KKEIGRCFKLTLKALETSVDLITTADFMCRFCANLDLPNMVQRAATHIAKKAVEMDIVPG 246
Query: 321 RSPVSIAAASIYMASLAMGIKKDKKDIGEVAGVAEGTLNVVYKLMRPHAEKLFPSNITPV 500
RSP+S+AAA+IYMAS A K+ +K+IG++AGVA+ T+ YKLM PHA KLFP +
Sbjct: 247 RSPISVAAAAIYMASQASEHKRSQKEIGDIAGVADVTIRQSYKLMYPHAAKLFPEDFKFT 306
Query: 501 VPLNQLP 521
P++QLP
Sbjct: 307 TPIDQLP 313
Score = 77.0 bits (188), Expect = 5e-14
Identities = 33/53 (62%), Positives = 46/53 (86%)
Frame = +2
Query: 11 RVHESKQLRGRNNEAVATSCLFMACRKLKVPRTFKEICAVSNVSKKDIGKIFK 169
+VH+ K L+GR+N+A A++CL++ACR+ VPRTFKEICAVS +SKK+IG+ FK
Sbjct: 143 QVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSKISKKEIGRCFK 195
>sp|Q9NHP7|TF2B_DROVI Transcription initiation factor IIB (General transcription factor
TFIIB)
Length = 293
Score = 112 bits (279), Expect = 1e-24
Identities = 59/127 (46%), Positives = 85/127 (66%), Gaps = 1/127 (0%)
Frame = +3
Query: 144 KRILEKFSKKFVHILSTSVGNATGEDFMARFCYQLGIPKL-EKIAKEISKKALDDNMVAG 320
K+ + + K + L TSV T DFM RFC L +P + ++ A I+KKA++ ++V G
Sbjct: 165 KKEIGRCFKLTLKALETSVDLITTADFMCRFCANLDLPNMVQRAATHIAKKAVEMDIVPG 224
Query: 321 RSPVSIAAASIYMASLAMGIKKDKKDIGEVAGVAEGTLNVVYKLMRPHAEKLFPSNITPV 500
RSP+S+AAA+IYMAS A K+ +K+IG++AGVA+ T+ YKLM PHA KLFP +
Sbjct: 225 RSPISVAAAAIYMASQASEHKRSQKEIGDIAGVADVTIRQSYKLMYPHAAKLFPEDFKFT 284
Query: 501 VPLNQLP 521
P++QLP
Sbjct: 285 TPIDQLP 291
Score = 77.0 bits (188), Expect = 5e-14
Identities = 33/53 (62%), Positives = 46/53 (86%)
Frame = +2
Query: 11 RVHESKQLRGRNNEAVATSCLFMACRKLKVPRTFKEICAVSNVSKKDIGKIFK 169
+VH+ K L+GR+N+A A++CL++ACR+ VPRTFKEICAVS +SKK+IG+ FK
Sbjct: 121 QVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSKISKKEIGRCFK 173
>sp|O16991|TF2B_CAEEL Transcription initiation factor IIB (General transcription factor
TFIIB)
Length = 306
Score = 105 bits (262), Expect = 1e-22
Identities = 62/135 (45%), Positives = 86/135 (63%), Gaps = 1/135 (0%)
Frame = +3
Query: 123 AQSRMFLKRILEKFSKKFVHILSTSVGNATGEDFMARFCYQLGIPK-LEKIAKEISKKAL 299
A SR+ K I F K V L T++ T DFM+RFC L +P ++ A I+K A+
Sbjct: 172 AVSRVSKKEIGRCF-KIIVRSLETNLEQITSADFMSRFCGNLSLPNSIQAAATRIAKCAV 230
Query: 300 DDNMVAGRSPVSIAAASIYMASLAMGIKKDKKDIGEVAGVAEGTLNVVYKLMRPHAEKLF 479
D ++VAGR+P+SIAAA+IYMAS A K+ K+IG+VAG AE T+ YKL+ P A +LF
Sbjct: 231 DMDLVAGRTPISIAAAAIYMASQASAEKRSAKEIGDVAGAAEITVRQTYKLLYPKALELF 290
Query: 480 PSNITPVVPLNQLPS 524
P + V P++ LP+
Sbjct: 291 PKDFRFVTPIDALPN 305
Score = 79.3 bits (194), Expect = 9e-15
Identities = 39/54 (72%), Positives = 45/54 (83%)
Frame = +2
Query: 14 VHESKQLRGRNNEAVATSCLFMACRKLKVPRTFKEICAVSNVSKKDIGKIFKKI 175
V ESK LRG+NNEA A +CL++ACRK VPRTFKEICAVS VSKK+IG+ FK I
Sbjct: 135 VLESKALRGKNNEAQAAACLYIACRKDGVPRTFKEICAVSRVSKKEIGRCFKII 188
Score = 29.6 bits (65), Expect = 8.6
Identities = 16/65 (24%), Positives = 35/65 (53%)
Frame = +3
Query: 261 LEKIAKEISKKALDDNMVAGRSPVSIAAASIYMASLAMGIKKDKKDIGEVAGVAEGTLNV 440
++ A I K L+ + G++ + AAA +Y+A G+ + K+I V+ V++ +
Sbjct: 124 IQDSASRIFKDVLESKALRGKNNEAQAAACLYIACRKDGVPRTFKEICAVSRVSKKEIGR 183
Query: 441 VYKLM 455
+K++
Sbjct: 184 CFKII 188
>sp|P62916|TF2B_RAT Transcription initiation factor IIB (General transcription factor
TFIIB) (RNA polymerase II alpha initiation factor)
sp|P62915|TF2B_MOUSE Transcription initiation factor IIB (General transcription factor
TFIIB) (RNA polymerase II alpha initiation factor)
Length = 316
Score = 102 bits (255), Expect = 8e-22
Identities = 60/134 (44%), Positives = 88/134 (65%), Gaps = 1/134 (0%)
Frame = +3
Query: 123 AQSRMFLKRILEKFSKKFVHILSTSVGNATGEDFMARFCYQLGIPK-LEKIAKEISKKAL 299
A SR+ K I F K + L TSV T DFM+RFC L +PK ++ A I++KA+
Sbjct: 182 AVSRISKKEIGRCF-KLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAV 240
Query: 300 DDNMVAGRSPVSIAAASIYMASLAMGIKKDKKDIGEVAGVAEGTLNVVYKLMRPHAEKLF 479
+ ++V GRSP+S+AAA+IYMAS A K+ +K+IG++AGVA+ T+ Y+L+ P A LF
Sbjct: 241 ELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLF 300
Query: 480 PSNITPVVPLNQLP 521
PS+ P+++LP
Sbjct: 301 PSDFKFDTPVDKLP 314
Score = 77.4 bits (189), Expect = 4e-14
Identities = 34/55 (61%), Positives = 47/55 (85%)
Frame = +2
Query: 11 RVHESKQLRGRNNEAVATSCLFMACRKLKVPRTFKEICAVSNVSKKDIGKIFKKI 175
+V+E K L+GR N+A+A++CL++ACR+ VPRTFKEICAVS +SKK+IG+ FK I
Sbjct: 144 QVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLI 198
>sp|Q00403|TF2B_HUMAN Transcription initiation factor IIB (General transcription factor
TFIIB) (S300-II)
sp|Q5R886|TF2B_PONPY Transcription initiation factor IIB (General transcription factor
TFIIB)
Length = 316
Score = 101 bits (252), Expect = 2e-21
Identities = 59/134 (44%), Positives = 88/134 (65%), Gaps = 1/134 (0%)
Frame = +3
Query: 123 AQSRMFLKRILEKFSKKFVHILSTSVGNATGEDFMARFCYQLGIPK-LEKIAKEISKKAL 299
A SR+ K I F K + L TSV T DFM+RFC L +PK ++ A I++KA+
Sbjct: 182 AVSRISKKEIGRCF-KLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAV 240
Query: 300 DDNMVAGRSPVSIAAASIYMASLAMGIKKDKKDIGEVAGVAEGTLNVVYKLMRPHAEKLF 479
+ ++V GRSP+S+AAA+IYMAS A K+ +K+IG++AGVA+ T+ Y+L+ P A LF
Sbjct: 241 ELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLF 300
Query: 480 PSNITPVVPLNQLP 521
P++ P+++LP
Sbjct: 301 PTDFKFDTPVDKLP 314
Score = 77.4 bits (189), Expect = 4e-14
Identities = 34/55 (61%), Positives = 47/55 (85%)
Frame = +2
Query: 11 RVHESKQLRGRNNEAVATSCLFMACRKLKVPRTFKEICAVSNVSKKDIGKIFKKI 175
+V+E K L+GR N+A+A++CL++ACR+ VPRTFKEICAVS +SKK+IG+ FK I
Sbjct: 144 QVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLI 198
>sp|P29054|TF2B_XENLA Transcription initiation factor IIB (General transcription factor
TFIIB)
Length = 316
Score = 100 bits (250), Expect = 3e-21
Identities = 58/134 (43%), Positives = 88/134 (65%), Gaps = 1/134 (0%)
Frame = +3
Query: 123 AQSRMFLKRILEKFSKKFVHILSTSVGNATGEDFMARFCYQLGIPK-LEKIAKEISKKAL 299
A SR+ K I F K + L T+V T DFM+RFC LG+ K ++ A I++KA+
Sbjct: 182 AVSRISKKEIGRCF-KLILKALETNVDLITTGDFMSRFCSNLGLTKQVQMAATHIARKAV 240
Query: 300 DDNMVAGRSPVSIAAASIYMASLAMGIKKDKKDIGEVAGVAEGTLNVVYKLMRPHAEKLF 479
+ ++V GRSP+S+AAA+IYMAS A K+ +K+IG++AGVA+ T+ Y+L+ P A LF
Sbjct: 241 ELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLF 300
Query: 480 PSNITPVVPLNQLP 521
P++ P+++LP
Sbjct: 301 PADFKFDTPVDKLP 314
Score = 78.6 bits (192), Expect = 2e-14
Identities = 34/55 (61%), Positives = 48/55 (87%)
Frame = +2
Query: 11 RVHESKQLRGRNNEAVATSCLFMACRKLKVPRTFKEICAVSNVSKKDIGKIFKKI 175
+V+E K L+GR+N+A+A++CL++ACR+ VPRTFKEICAVS +SKK+IG+ FK I
Sbjct: 144 QVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLI 198
Score = 30.0 bits (66), Expect = 6.6
Identities = 15/56 (26%), Positives = 32/56 (57%)
Frame = +3
Query: 288 KKALDDNMVAGRSPVSIAAASIYMASLAMGIKKDKKDIGEVAGVAEGTLNVVYKLM 455
K+ + + GRS +IA+A +Y+A G+ + K+I V+ +++ + +KL+
Sbjct: 143 KQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLI 198
>sp|O13749|TF2B_SCHPO Transcription initiation factor IIB (General transcription factor
TFIIB)
Length = 340
Score = 77.8 bits (190), Expect = 3e-14
Identities = 40/91 (43%), Positives = 60/91 (65%), Gaps = 1/91 (1%)
Frame = +3
Query: 204 NATGEDFMARFCYQLGIP-KLEKIAKEISKKALDDNMVAGRSPVSIAAASIYMASLAMGI 380
+ + ED M RFC +L +P ++ A E++++A +AGRSP+SIAA+ IYM S MG
Sbjct: 229 STSAEDLMVRFCNRLMLPMSVQSAAAELARRAGLQGTLAGRSPISIAASGIYMISALMGY 288
Query: 381 KKDKKDIGEVAGVAEGTLNVVYKLMRPHAEK 473
K K+I EV GV++ T+ + YKL+ HAE+
Sbjct: 289 PKTPKEISEVTGVSDSTIRIAYKLL--HAER 317
Score = 66.6 bits (161), Expect = 6e-11
Identities = 25/55 (45%), Positives = 45/55 (81%)
Frame = +2
Query: 11 RVHESKQLRGRNNEAVATSCLFMACRKLKVPRTFKEICAVSNVSKKDIGKIFKKI 175
RV + K L+G++++++ +C+++ACR+ KVPRTF EIC ++NV KK+IG+++K +
Sbjct: 148 RVDDHKALKGKSSQSIIAACIYIACRQGKVPRTFMEICTLTNVPKKEIGRVYKTL 202
Score = 33.9 bits (76), Expect = 0.46
Identities = 21/75 (28%), Positives = 38/75 (50%), Gaps = 1/75 (1%)
Frame = +3
Query: 237 CYQLGIPK-LEKIAKEISKKALDDNMVAGRSPVSIAAASIYMASLAMGIKKDKKDIGEVA 413
C + +PK + AK++ K+ D + G+S SI AA IY+A + + +I +
Sbjct: 129 CDAISLPKVIADTAKQLYKRVDDHKALKGKSSQSIIAACIYIACRQGKVPRTFMEICTLT 188
Query: 414 GVAEGTLNVVYKLMR 458
V + + VYK ++
Sbjct: 189 NVPKKEIGRVYKTLQ 203
>sp|Q05959|TF2B_KLULA Transcription initiation factor IIB (General transcription factor
TFIIB)
Length = 357
Score = 74.3 bits (181), Expect = 3e-13
Identities = 49/142 (34%), Positives = 81/142 (57%), Gaps = 6/142 (4%)
Frame = +3
Query: 114 KKYAQSRMFLKRIL-EKFSKKFVHILSTSV--GNATGEDFMARFCYQLGIP-KLEKIAKE 281
K+ ++ +K IL EK + F +I ++++ G + E F+ RFC LG+ ++ A+
Sbjct: 205 KEIGKTFTIIKNILREKNDRGFANIDTSNITTGQTSAETFIPRFCSHLGLSVQVANAAEY 264
Query: 282 ISKKALDDNMVAGRSPVSIAAASIYMASLAMGIKKDKKDIGEVAGVAEGTLNVVYKLMRP 461
I+K + D N++AGRSP++IAAA+IYMA+L + I + V EGT+ YK +
Sbjct: 265 IAKHSKDVNVLAGRSPITIAAAAIYMATLLFKLNISPTRISQTLQVTEGTVKGGYKALYE 324
Query: 462 HAEKLFPSNI--TPVVPLNQLP 521
H EK+ + + VV + LP
Sbjct: 325 HKEKVVDPQLIESGVVKWDNLP 346
Score = 51.2 bits (121), Expect = 3e-06
Identities = 21/49 (42%), Positives = 36/49 (73%)
Frame = +2
Query: 20 ESKQLRGRNNEAVATSCLFMACRKLKVPRTFKEICAVSNVSKKDIGKIF 166
E + L+G++ E++ S + + CR+ +V R+FKEI +++NV KK+IGK F
Sbjct: 163 EERVLKGKSQESIMASVILVGCRRAEVGRSFKEILSLTNVRKKEIGKTF 211
>sp|O28970|TF2B_ARCFU Transcription initiation factor IIB (TFIIB)
Length = 326
Score = 67.4 bits (163), Expect = 4e-11
Identities = 36/87 (41%), Positives = 59/87 (67%), Gaps = 1/87 (1%)
Frame = +3
Query: 219 DFMARFCYQLGIP-KLEKIAKEISKKALDDNMVAGRSPVSIAAASIYMASLAMGIKKDKK 395
D++ RFC LG+ +++K A EI KKA + + +GR P +AAA++Y+AS+ +G ++ ++
Sbjct: 237 DYIPRFCAALGLSGEVQKKAIEIIKKAEERELTSGRGPTGVAAAALYVASILLGERRTQR 296
Query: 396 DIGEVAGVAEGTLNVVYKLMRPHAEKL 476
++ EVAGV E T+ YK + AEKL
Sbjct: 297 EVAEVAGVTEVTIRNRYKEL---AEKL 320
Score = 48.1 bits (113), Expect = 2e-05
Identities = 22/52 (42%), Positives = 32/52 (61%)
Frame = +2
Query: 20 ESKQLRGRNNEAVATSCLFMACRKLKVPRTFKEICAVSNVSKKDIGKIFKKI 175
E +RGR+ E V + L+ ACR+ VPRT EI S V +K+IG+ ++ I
Sbjct: 171 EKNLIRGRSIEGVVAAALYAACRQAGVPRTLDEIATYSRVDRKEIGRTYRFI 222
>sp|P29055|TF2B_YEAST Transcription initiation factor IIB (General transcription factor
TFIIB) (Transcription factor E)
Length = 345
Score = 67.0 bits (162), Expect = 5e-11
Identities = 43/140 (30%), Positives = 73/140 (52%), Gaps = 4/140 (2%)
Frame = +3
Query: 114 KKYAQSRMFLKRILE-KFSKKFVHILSTSVGNATGEDFMARFCYQLGIP-KLEKIAKEIS 287
K++ ++ +K IL K F+ I + ++ A ++ RFC LG+P ++ A+ +
Sbjct: 201 KEFGKTLNIMKNILRGKSEDGFLKIDTDNMSGAQNLTYIPRFCSHLGLPMQVTTSAEYTA 260
Query: 288 KKALDDNMVAGRSPVSIAAASIYMASLAMGIKKDKKDIGEVAGVAEGTLNVVYKLMRPHA 467
KK + +AG+SP++IA SIY+ L I +G+ V EGT+ YK++ H
Sbjct: 261 KKCKEIKEIAGKSPITIAVVSIYLNILLFQIPITAAKVGQTLQVTEGTIKSGYKILYEHR 320
Query: 468 EKLFPSNI--TPVVPLNQLP 521
+KL + VV L+ LP
Sbjct: 321 DKLVDPQLIANGVVSLDNLP 340
Score = 45.4 bits (106), Expect = 2e-04
Identities = 18/48 (37%), Positives = 33/48 (68%)
Frame = +2
Query: 17 HESKQLRGRNNEAVATSCLFMACRKLKVPRTFKEICAVSNVSKKDIGK 160
H+ K L+G++ E++ + + + CR+ +V RTFKEI ++ +V K+ GK
Sbjct: 158 HDEKTLKGKSMESIMAASILIGCRRAEVARTFKEIQSLIHVKTKEFGK 205
Score = 35.0 bits (79), Expect = 0.20
Identities = 32/114 (28%), Positives = 52/114 (45%), Gaps = 9/114 (7%)
Frame = +3
Query: 237 CYQLGIPKLEK-IAKEISKKALDDNMVAGRSPVSIAAASIYMASLAMGIKKDKKDIGEVA 413
C +PK+ K AKE K D+ + G+S SI AASI + + + K+I +
Sbjct: 137 CDAAELPKIVKDCAKEAYKLCHDEKTLKGKSMESIMAASILIGCRRAEVARTFKEIQSLI 196
Query: 414 GVAE----GTLNVVYKLMRPHAE----KLFPSNITPVVPLNQLPSF*LSQCYHV 551
V TLN++ ++R +E K+ N++ L +P F C H+
Sbjct: 197 HVKTKEFGKTLNIMKNILRGKSEDGFLKIDTDNMSGAQNLTYIPRF----CSHL 246
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,969,249
Number of Sequences: 369166
Number of extensions: 1473040
Number of successful extensions: 4319
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4301
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6510836890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)