Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_O03
(705 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9H477|RBSK_HUMAN Ribokinase 136 5e-32
sp|P44331|RBSK_HAEIN Ribokinase 107 3e-23
sp|O60116|RBSK_SCHPO Putative ribokinase 88 2e-17
sp|P0A9J6|RBSK_ECOLI Ribokinase >gi|71152935|sp|P0A9J7|RBSK... 87 6e-17
sp|Q9CF42|RBSK_LACLA Ribokinase 72 2e-12
sp|Q9K6K1|RBSK_BACHD Ribokinase 67 5e-11
sp|P25332|RBSK_YEAST Probable ribokinase 51 3e-06
sp|P36945|RBSK_BACSU Ribokinase 50 4e-06
sp|Q9ZKZ0|RFAE_HELPJ ADP-heptose synthase 50 6e-06
sp|Q9LZG0|ADK2_ARATH Adenosine kinase 2 (AK 2) (Adenosine 5... 48 2e-05
>sp|Q9H477|RBSK_HUMAN Ribokinase
Length = 322
Score = 136 bits (343), Expect = 5e-32
Identities = 83/215 (38%), Positives = 124/215 (57%), Gaps = 1/215 (0%)
Frame = +2
Query: 26 AKSSVAFVTVDKSGSNHIVLASGADQLLSVSDIVECTELIATSKVLISNFEIPMDACLEA 205
A + A + V+ G N IV+ +GA+ LL+ D+ +I+ +KV++ EI LEA
Sbjct: 104 AATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEA 163
Query: 206 LSIAKRFHVKTILVPAPVPKDFTANMIPDVDIICPNEIEAGQMLGMKIDSIEAAKEAIEK 385
L++A+R VKT+ PAP D D+ C NE EA + G+ + S A EA
Sbjct: 164 LTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALV 223
Query: 386 FYETRNVKWPIITMGAKGAVV-GIKPGLSRHVEAPKVESVDTTGAGDSFVGSLAYQLFKN 562
+ R + IIT+GA+G VV +H+ KV++VDTTGAGDSFVG+LA+ L
Sbjct: 224 LLK-RGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYY 282
Query: 563 PSQSIDSMVSRSIFVASMSTLKEGTRTSYVSAKDL 667
P+ S++ M++RS F+A++S GT++SY KDL
Sbjct: 283 PNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDL 317
>sp|P44331|RBSK_HAEIN Ribokinase
Length = 306
Score = 107 bits (267), Expect = 3e-23
Identities = 72/214 (33%), Positives = 117/214 (54%)
Frame = +2
Query: 5 HVKISHKAKSSVAFVTVDKSGSNHIVLASGADQLLSVSDIVECTELIATSKVLISNFEIP 184
H+ + + +AF+ V KS N IVLASGA+ LS + + IA S L+ E P
Sbjct: 84 HINTVSQEMTGMAFIQVAKSSENSIVLASGANSHLSEMVVRQSEAQIAQSDCLLMQLETP 143
Query: 185 MDACLEALSIAKRFHVKTILVPAPVPKDFTANMIPDVDIICPNEIEAGQMLGMKIDSIEA 364
+ A IAK+ VK +L PAP + + ++ +DII PNE EA + G+++ ++
Sbjct: 144 LSGVELAAQIAKKNGVKVVLNPAPA-QILSDELLSLIDIITPNETEAEILTGVEVADEQS 202
Query: 365 AKEAIEKFYETRNVKWPIITMGAKGAVVGIKPGLSRHVEAPKVESVDTTGAGDSFVGSLA 544
A +A F++ + ++ +IT+GAKG V K G SR ++ V+++DTT AGD+F G
Sbjct: 203 AVKAASVFHD-KGIETVMITLGAKGVFVSRK-GKSRIIKGFCVQAIDTTAAGDTFNGGFV 260
Query: 545 YQLFKNPSQSIDSMVSRSIFVASMSTLKEGTRTS 646
L + +S D + A++S K+G ++S
Sbjct: 261 TALLE--EKSFDEAIRFGQAAAAISVTKKGAQSS 292
>sp|O60116|RBSK_SCHPO Putative ribokinase
Length = 318
Score = 88.2 bits (217), Expect = 2e-17
Identities = 61/187 (32%), Positives = 102/187 (54%), Gaps = 6/187 (3%)
Frame = +2
Query: 2 DHVKISHKAKSSVAFVTVDKSGSNHIVLASGADQLLSVSDIVECTELIATSKVLISNFEI 181
D+VK + VA + V+++G N I+L+ GA+ + + + + I+T +LI EI
Sbjct: 88 DNVKKIENKSTGVAMIIVEETGENRILLSEGANGNVDTAFVKAMEQRISTCNLLIMQLEI 147
Query: 182 PMDACLEALSIAKRFHVKTILVPAP-VPKDFTANMIPDVDIICPNEIEAGQMLGM----- 343
P++A AL IA + V ++ PAP +P + +MI + PNE EA +L
Sbjct: 148 PLEAVEIALQIAHKHGVDVLMNPAPAIP--LSHDMISYCAYLVPNEHEAAILLNQADSPA 205
Query: 344 KIDSIEAAKEAIEKFYETRNVKWPIITMGAKGAVVGIKPGLSRHVEAPKVESVDTTGAGD 523
+++++A + F + V IIT+G++GA G S V A KV++VDTT AGD
Sbjct: 206 TLENVDAYASKLLSFGVRKAV---IITLGSQGAYYKSANGESALVSACKVKAVDTTAAGD 262
Query: 524 SFVGSLA 544
+F+G+ +
Sbjct: 263 TFIGAFS 269
>sp|P0A9J6|RBSK_ECOLI Ribokinase
sp|P0A9J7|RBSK_ECO57 Ribokinase
Length = 309
Score = 86.7 bits (213), Expect = 6e-17
Identities = 58/213 (27%), Positives = 113/213 (53%)
Frame = +2
Query: 8 VKISHKAKSSVAFVTVDKSGSNHIVLASGADQLLSVSDIVECTELIATSKVLISNFEIPM 187
V + + VA + V+ G N I + +GA+ LS + + E IA + L+ E P+
Sbjct: 87 VSVIKGESTGVALIFVNGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPL 146
Query: 188 DACLEALSIAKRFHVKTILVPAPVPKDFTANMIPDVDIICPNEIEAGQMLGMKIDSIEAA 367
++ + A IA + L PAP ++ ++ VDII PNE EA ++ G+++++ E A
Sbjct: 147 ESVMAAAKIAHQNKTIVALNPAPA-RELPDELLALVDIITPNETEAEKLTGIRVENDEDA 205
Query: 368 KEAIEKFYETRNVKWPIITMGAKGAVVGIKPGLSRHVEAPKVESVDTTGAGDSFVGSLAY 547
+A + +E + ++ +IT+G++G + G + V +V++VDT AGD+F G+L
Sbjct: 206 AKAAQVLHE-KGIRTVLITLGSRGVWASVN-GEGQRVPGFRVQAVDTIAAGDTFNGALIT 263
Query: 548 QLFKNPSQSIDSMVSRSIFVASMSTLKEGTRTS 646
L + + + + + A+++ ++G + S
Sbjct: 264 ALLE--EKPLPEAIRFAHAAAAIAVTRKGAQPS 294
>sp|Q9CF42|RBSK_LACLA Ribokinase
Length = 300
Score = 71.6 bits (174), Expect = 2e-12
Identities = 59/190 (31%), Positives = 91/190 (47%)
Frame = +2
Query: 71 NHIVLASGADQLLSVSDIVECTELIATSKVLISNFEIPMDACLEALSIAKRFHVKTILVP 250
N I++ GA+ + S + + E I S+++I EIP + L K +K + P
Sbjct: 104 NRIIIIPGANNEVLPSYLADLWEKIKESQLVILQNEIPHETNLAIAKFCKENAIKVLYNP 163
Query: 251 APVPKDFTANMIPDVDIICPNEIEAGQMLGMKIDSIEAAKEAIEKFYETRNVKWPIITMG 430
AP K MI VD I PNE E ++ A E I K Y R I+T+G
Sbjct: 164 APARKT-DLEMIDFVDYITPNEHECKELFP------NLALEEILKKYSNRL----IVTLG 212
Query: 431 AKGAVVGIKPGLSRHVEAPKVESVDTTGAGDSFVGSLAYQLFKNPSQSIDSMVSRSIFVA 610
++G + L + + A K + VDTTGAGD+F G+ A+ L +N SI + ++ +
Sbjct: 213 SEGVIFHDGETLQK-IPAIKAKVVDTTGAGDTFNGAFAFGLTEN--LSISDSIRLAVVAS 269
Query: 611 SMSTLKEGTR 640
+S K G +
Sbjct: 270 HLSIQKFGAQ 279
>sp|Q9K6K1|RBSK_BACHD Ribokinase
Length = 294
Score = 67.0 bits (162), Expect = 5e-11
Identities = 58/184 (31%), Positives = 84/184 (45%)
Frame = +2
Query: 2 DHVKISHKAKSSVAFVTVDKSGSNHIVLASGADQLLSVSDIVECTELIATSKVLISNFEI 181
D VK S VA + + N I++ GA++ ++ + + +A S V++ EI
Sbjct: 84 DSVKPVTDCTSGVATILLSDR-DNRIIVTKGANEHVTPDYVAAFEQELAASDVVLLQLEI 142
Query: 182 PMDACLEALSIAKRFHVKTILVPAPVPKDFTANMIPDVDIICPNEIEAGQMLGMKIDSIE 361
P++ L + HV T+L PAP K A D I PNE E Q+ G + D+
Sbjct: 143 PLETVAYVLEFCAKHHVTTVLNPAPAQKLPDAAW-TDATYISPNENECLQLFGDEPDANL 201
Query: 362 AAKEAIEKFYETRNVKWPIITMGAKGAVVGIKPGLSRHVEAPKVESVDTTGAGDSFVGSL 541
K I+T GA G V + VE+ +VE VDTTGAGD+F G+
Sbjct: 202 RQK--------------LIMTKGADG-VQFYENDEQVQVESFRVEPVDTTGAGDTFNGAF 246
Query: 542 AYQL 553
A L
Sbjct: 247 AVAL 250
>sp|P25332|RBSK_YEAST Probable ribokinase
Length = 333
Score = 50.8 bits (120), Expect = 3e-06
Identities = 58/251 (23%), Positives = 108/251 (43%), Gaps = 29/251 (11%)
Frame = +2
Query: 5 HVKISHKAKSSVAFVTVDKS--GSNHIVLASGADQLLSVSDIVECTELIATSK----VLI 166
HV + A + +++ G N I++ GA+ ++ D + E+ K ++
Sbjct: 87 HVGTYEGINTGTATILIEEKAGGQNRILIVEGANSK-TIYDPKQLCEIFPEGKEEEEYVV 145
Query: 167 SNFEIPMDACLEALSIAKRFHVKTILVPAPVPKDFTANMIPD---VDIICPNEIEAGQML 337
EIP + A R + + + P+P F A D VD++ NEIE Q++
Sbjct: 146 FQHEIPDPLSIIKWIHANRPNFQIVYNPSP----FKAMPKKDWELVDLLVVNEIEGLQIV 201
Query: 338 GMKIDS--IEAAKEAI----------------EKFYETRNVKWPIITMGAKGAVVGI--K 457
D+ +E +E I EK + ++T+G++G +
Sbjct: 202 ESVFDNELVEEIREKIKDDFLGEYRKICELLYEKLMNRKKRGIVVMTLGSRGVLFCSHES 261
Query: 458 PGLSRHVEAPKVESVDTTGAGDSFVGSLAYQLFKNPSQSIDSMVSRSIFVASMSTLKEGT 637
P + V VDTTGAGD+F+G L QL++ +++ + + S +S++ ++G
Sbjct: 262 PEVQFLPAIQNVSVVDTTGAGDTFLGGLVTQLYQ--GETLSTAIKFSTLASSLTIQRKGA 319
Query: 638 RTSYVSAKDLE 670
S KD++
Sbjct: 320 AESMPLYKDVQ 330
>sp|P36945|RBSK_BACSU Ribokinase
Length = 293
Score = 50.4 bits (119), Expect = 4e-06
Identities = 57/217 (26%), Positives = 92/217 (42%), Gaps = 9/217 (4%)
Frame = +2
Query: 47 VTVDKSGSNHIVLASGADQLLSVS----DIVECTELIATSKV-----LISNFEIPMDACL 199
VT +SG+ HIVLA G + ++ V DI L A ++ ++ EIP +
Sbjct: 87 VTHTESGTAHIVLAEGDNSIVVVKGANDDITPAYALNALEQIEKVDMVLIQQEIPEETVD 146
Query: 200 EALSIAKRFHVKTILVPAPVPKDFTANMIPDVDIICPNEIEAGQMLGMKIDSIEAAKEAI 379
E + IL PAP + I + PNE EA +L ++ EA
Sbjct: 147 EVCKYCHSHDIPIILNPAPA-RPLKQETIDHATYLTPNEHEAS-ILFPELTISEALALYP 204
Query: 380 EKFYETRNVKWPIITMGAKGAVVGIKPGLSRHVEAPKVESVDTTGAGDSFVGSLAYQLFK 559
K + T + + G+K ++ P VE VDTTGAGD+F + A L +
Sbjct: 205 AKLFITEGKQGVRYSAGSKEVLIPSFP----------VEPVDTTGAGDTFNAAFAVALAE 254
Query: 560 NPSQSIDSMVSRSIFVASMSTLKEGTRTSYVSAKDLE 670
+ I++ + + AS+S G + + ++E
Sbjct: 255 --GKDIEAALRFANRAASLSVCSFGAQGGMPTRNEVE 289
>sp|Q9ZKZ0|RFAE_HELPJ ADP-heptose synthase
Length = 463
Score = 50.1 bits (118), Expect = 6e-06
Identities = 35/123 (28%), Positives = 61/123 (49%), Gaps = 2/123 (1%)
Frame = +2
Query: 206 LSIAKRFHVKTILVPAPVPKDFTANMIPDVDIICPNEIEAGQMLGMKIDSIEAAKEAIEK 385
+++A + H +++ P KD++ +I PN E Q L +K+DS +A++
Sbjct: 160 ITLANKHH--KLILCDPKGKDYSK--YSHASLITPNRAELEQALHLKLDSHANLSKALQI 215
Query: 386 FYETRNVKWPIITMGAKGAVVGIKPGLSRHVEAPKV--ESVDTTGAGDSFVGSLAYQLFK 559
ET ++ P++T+ +G K L V P + E D TGAGD+ + SL L +
Sbjct: 216 LQETYHIAMPLVTLSEQGIAFLEKGEL---VNCPTIAKEVYDVTGAGDTVIASLTLSLLE 272
Query: 560 NPS 568
+ S
Sbjct: 273 SKS 275
>sp|Q9LZG0|ADK2_ARATH Adenosine kinase 2 (AK 2) (Adenosine 5'-phosphotransferase 2)
Length = 345
Score = 48.1 bits (113), Expect = 2e-05
Identities = 36/131 (27%), Positives = 61/131 (46%), Gaps = 7/131 (5%)
Frame = +2
Query: 263 KDFTANMIPDVDIICPNEIEA---GQMLGMKIDSIEAAKEAIEKFYETRNV--KWPIITM 427
KD +P +D + NE EA ++ G + + +E I + + + +IT
Sbjct: 207 KDVQEKFLPYMDFVFGNETEARTFSRVHGWETEDVEQIAIKISQLPKATGTYKRTTVITQ 266
Query: 428 GAKGAVVGIKPGLSRH--VEAPKVESVDTTGAGDSFVGSLAYQLFKNPSQSIDSMVSRSI 601
GA VV + ++ + PK + VDT GAGD+FVG QL K +SI+ V
Sbjct: 267 GADPVVVAEDGKVKKYPVIPLPKEKLVDTNGAGDAFVGGFMSQLVK--EKSIEECVKAGC 324
Query: 602 FVASMSTLKEG 634
+ +++ + G
Sbjct: 325 YASNVVIQRSG 335
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,929,894
Number of Sequences: 369166
Number of extensions: 1593499
Number of successful extensions: 4270
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4252
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6170718545
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)