Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_N10
(524 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP... 194 1e-49
sp|Q05893|PPCK_ASCSU Phosphoenolpyruvate carboxykinase [GTP... 183 2e-46
sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mi... 177 1e-44
sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mi... 176 4e-44
sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP... 167 1e-41
sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cyto... 164 2e-40
sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cy... 160 2e-39
sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cy... 154 1e-37
sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cy... 154 1e-37
sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cy... 149 3e-36
>sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 619
Score = 194 bits (493), Expect = 1e-49
Identities = 88/145 (60%), Positives = 112/145 (77%), Gaps = 2/145 (1%)
Frame = +3
Query: 3 MHDPMAMRPFMGYNFGKYLQHWLDLEKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRV 182
MHDPMAMRPFMGYNFG YLQHW+DL K GRK+P I+HVNWFR + KF+WPG+G+NIRV
Sbjct: 472 MHDPMAMRPFMGYNFGHYLQHWIDLNKDGRKVPKIYHVNWFRRDANNKFLWPGYGQNIRV 531
Query: 183 IDWMCRRVNGE-DIAEPSPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKF 356
IDW+ RR++GE DI +PIG++PK G IN +GL D +WDE+ S+PK Y + D +E KF
Sbjct: 532 IDWIVRRLDGEPDIGVDTPIGIVPKKGAINASGLPDIQWDELMSVPKEYWTNDAKEIRKF 591
Query: 357 LREQTGEDLPAGIIQELNEQDKRIH 431
L EQ G DLP I E++ Q++RI+
Sbjct: 592 LEEQVGPDLPKEIRAEMDAQEERIN 616
>sp|Q05893|PPCK_ASCSU Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 643
Score = 183 bits (465), Expect = 2e-46
Identities = 84/144 (58%), Positives = 107/144 (74%), Gaps = 2/144 (1%)
Frame = +3
Query: 3 MHDPMAMRPFMGYNFGKYLQHWLDLEKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRV 182
MHDPMAMRPFMGYNFG+Y++HW+ L +P K+P IFHVNWFR + KF+WPG+G+NIRV
Sbjct: 495 MHDPMAMRPFMGYNFGRYMRHWMKLGQPPHKVPKIFHVNWFRQSADHKFLWPGYGDNIRV 554
Query: 183 IDWMCRRVNGE-DIAEPSPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKF 356
IDW+ RR +G+ IAE +PIG +PK GTIN+ GL + WDE+ S+PK+Y ED+ ET F
Sbjct: 555 IDWILRRCSGDATIAEETPIGFIPKKGTINLEGLPNVNWDELMSIPKSYWLEDMVETKTF 614
Query: 357 LREQTGEDLPAGIIQELNEQDKRI 428
Q G DLP I +EL Q +RI
Sbjct: 615 FENQVGSDLPPEIAKELEAQTERI 638
>sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 177 bits (450), Expect = 1e-44
Identities = 77/147 (52%), Positives = 108/147 (73%), Gaps = 2/147 (1%)
Frame = +3
Query: 3 MHDPMAMRPFMGYNFGKYLQHWLDLE-KPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIR 179
MHDP AMRPF GYNFG YL+HWL +E + G ++P IFHVNWFR ++ G F+WPGFGEN R
Sbjct: 494 MHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENAR 553
Query: 180 VIDWMCRRVNGEDIAEPSPIGLLPKHGTINVTGLDA-KWDEMFSLPKAYLSEDVQETMKF 356
V+DW+CRR+ GED A +PIGL+PK G ++++GL A ++FSLPK + ++V++ +
Sbjct: 554 VLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSY 613
Query: 357 LREQTGEDLPAGIIQELNEQDKRIHNM 437
L EQ +DLP ++ EL ++R+H M
Sbjct: 614 LTEQVNQDLPKEVLAELEALERRVHKM 640
>sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 176 bits (445), Expect = 4e-44
Identities = 75/147 (51%), Positives = 109/147 (74%), Gaps = 2/147 (1%)
Frame = +3
Query: 3 MHDPMAMRPFMGYNFGKYLQHWLDLE-KPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIR 179
MHDP AMRPF GYNFG+YL+HWL +E + G ++P IFHVNWFR ++ G+F+WPGFGEN R
Sbjct: 494 MHDPFAMRPFFGYNFGRYLEHWLSMEGQKGARLPRIFHVNWFRRDEAGRFLWPGFGENAR 553
Query: 180 VIDWMCRRVNGEDIAEPSPIGLLPKHGTINVTGLDA-KWDEMFSLPKAYLSEDVQETMKF 356
V+DW+CRR+ GED A+ +PIGL+PK G ++++GL A ++FS+PK + ++V++ +
Sbjct: 554 VLDWICRRLEGEDSAQETPIGLVPKEGALDLSGLSAVDTSQLFSIPKDFWEQEVRDIRGY 613
Query: 357 LREQTGEDLPAGIIQELNEQDKRIHNM 437
L EQ +DLP ++ EL + R+ M
Sbjct: 614 LTEQVNQDLPKEVLAELEALEGRVQKM 640
>sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 647
Score = 167 bits (424), Expect = 1e-41
Identities = 77/147 (52%), Positives = 103/147 (70%), Gaps = 2/147 (1%)
Frame = +3
Query: 3 MHDPMAMRPFMGYNFGKYLQHWLDLEKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIRV 182
MHDP AMRPF GYNFG Y+ HWL +EK G+ +P IFHVNWFR + +GKF+WPG+GEN RV
Sbjct: 502 MHDPFAMRPFFGYNFGDYVAHWLSMEKRGQ-VPKIFHVNWFRKSAEGKFMWPGYGENSRV 560
Query: 183 IDWMCRRVNGEDIAEPSPIGLLPKHGTINVTGLDAKWD--EMFSLPKAYLSEDVQETMKF 356
++W+ RRVNGE S IG +P G +N+ G+ K D E+FSLPK + S++V++ +
Sbjct: 561 LEWILRRVNGESCYVDSAIGHIPAEGALNLDGMKDKVDVKEIFSLPKEFWSQEVKDIRTY 620
Query: 357 LREQTGEDLPAGIIQELNEQDKRIHNM 437
Q G DLPA I Q+L+E R+ N+
Sbjct: 621 FESQVGADLPASIYQQLDELSSRVDNL 647
>sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 164 bits (414), Expect = 2e-40
Identities = 76/147 (51%), Positives = 100/147 (68%), Gaps = 2/147 (1%)
Frame = +3
Query: 3 MHDPMAMRPFMGYNFGKYLQHWLDL-EKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIR 179
MHDP AMRPF GYNFGKYL HWL + +P K+P IFHVNWFR + GKF+WPGFGEN R
Sbjct: 476 MHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSR 535
Query: 180 VIDWMCRRVNGEDIAEPSPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKF 356
V++WM R+ GED A+ +PIG +PK +N+ GL D +E+F + K + ++V+E K+
Sbjct: 536 VLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKY 595
Query: 357 LREQTGEDLPAGIIQELNEQDKRIHNM 437
L +Q DLP I +EL +RI M
Sbjct: 596 LEDQVNADLPYEIERELRALKQRISQM 622
>sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 160 bits (405), Expect = 2e-39
Identities = 74/147 (50%), Positives = 99/147 (67%), Gaps = 2/147 (1%)
Frame = +3
Query: 3 MHDPMAMRPFMGYNFGKYLQHWLDL-EKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIR 179
MHDP AMRPF GYNFGKYL HWL + +P K+P IFHVNWFR + GKF+WPGFGEN R
Sbjct: 476 MHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKDGKFLWPGFGENSR 535
Query: 180 VIDWMCRRVNGEDIAEPSPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKF 356
V++WM R+ GED A+ +PIG +PK +N+ GL +E+F + K + ++V+E ++
Sbjct: 536 VLEWMFGRIEGEDSAKLTPIGYIPKENALNLKGLGGVNVEELFGISKEFWEKEVEEIDRY 595
Query: 357 LREQTGEDLPAGIIQELNEQDKRIHNM 437
L +Q DLP I +EL +RI M
Sbjct: 596 LEDQVNTDLPYEIERELRALKQRISQM 622
>sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 154 bits (390), Expect = 1e-37
Identities = 71/147 (48%), Positives = 99/147 (67%), Gaps = 2/147 (1%)
Frame = +3
Query: 3 MHDPMAMRPFMGYNFGKYLQHWLDL-EKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIR 179
MHDP AMRPF GYNFGKYL HWL + + P K+P IFHVNWFR + +GKF+WPGFGEN R
Sbjct: 476 MHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSR 535
Query: 180 VIDWMCRRVNGEDIAEPSPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKF 356
V++WM R++G+ + +PIG +PK +N+ GL E+FS+ K + ++V++ K+
Sbjct: 536 VLEWMFNRIDGKASTKLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKY 595
Query: 357 LREQTGEDLPAGIIQELNEQDKRIHNM 437
L +Q DLP I +E+ +RI M
Sbjct: 596 LEDQVNADLPCEIEREILALKQRISQM 622
>sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 154 bits (390), Expect = 1e-37
Identities = 72/147 (48%), Positives = 100/147 (68%), Gaps = 2/147 (1%)
Frame = +3
Query: 3 MHDPMAMRPFMGYNFGKYLQHWLDL-EKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIR 179
MHDP AMRPF GYNFGKYL HWL + + P K+P IFHVNWFR + +GKF+WPGFGEN R
Sbjct: 476 MHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSR 535
Query: 180 VIDWMCRRVNGEDIAEPSPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKF 356
V++WM R++G+ A+ +PIG +PK +N+ GL E+FS+ K + ++V++ K+
Sbjct: 536 VLEWMFNRIDGKAGAKLTPIGYIPKEDALNLKGLGHINVMELFSISKEFWEKEVEDIEKY 595
Query: 357 LREQTGEDLPAGIIQELNEQDKRIHNM 437
L +Q DLP I +E+ +RI M
Sbjct: 596 LEDQVNADLPCEIEREILALKQRISQM 622
>sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 149 bits (377), Expect = 3e-36
Identities = 71/147 (48%), Positives = 95/147 (64%), Gaps = 2/147 (1%)
Frame = +3
Query: 3 MHDPMAMRPFMGYNFGKYLQHWLDL-EKPGRKMPLIFHVNWFRLNDKGKFVWPGFGENIR 179
MHDP AM PF GYNFG+YL HWL + ++P K+P IFHVNWFR + G+F+WPGFGEN R
Sbjct: 476 MHDPFAMGPFFGYNFGQYLAHWLSMAQRPAAKLPKIFHVNWFRKDKAGRFLWPGFGENSR 535
Query: 180 VIDWMCRRVNGEDIAEPSPIGLLPKHGTINVTGL-DAKWDEMFSLPKAYLSEDVQETMKF 356
V++WM RV GE A +PIG +P +++ GL D E+F + K + E+V+E K+
Sbjct: 536 VLEWMFNRVVGEGGASVTPIGYIPDEDALDLRGLGDVDVKELFHISKEFWEEEVEEIQKY 595
Query: 357 LREQTGEDLPAGIIQELNEQDKRIHNM 437
L EQ DLP I ++ +RI M
Sbjct: 596 LEEQVNVDLPPEIKNQVLALKQRISQM 622
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,224,268
Number of Sequences: 369166
Number of extensions: 1487408
Number of successful extensions: 3927
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3767
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3861
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3502236525
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)