Planarian EST Database


Dr_sW_014_M14

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_014_M14
         (450 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q6F2U9|SYK_ORYSA  Lysyl-tRNA synthetase (Lysine--tRNA lig...   138   5e-33
sp|Q9ZPI1|SYK_ARATH  Lysyl-tRNA synthetase (Lysine--tRNA lig...   138   6e-33
sp|P37879|SYK_CRIGR  Lysyl-tRNA synthetase (Lysine--tRNA lig...   132   3e-31
sp|Q99MN1|SYK_MOUSE  Lysyl-tRNA synthetase (Lysine--tRNA lig...   132   4e-31
sp|Q15046|SYK_HUMAN  Lysyl-tRNA synthetase (Lysine--tRNA lig...   130   1e-30
sp|P15180|SYKC_YEAST  Lysyl-tRNA synthetase, cytoplasmic (Ly...   129   4e-30
sp|Q22099|SYK_CAEEL  Lysyl-tRNA synthetase (Lysine--tRNA lig...   124   7e-29
sp|Q43776|SYK_LYCES  Lysyl-tRNA synthetase (Lysine--tRNA lig...   121   6e-28
sp|O67258|SYK_AQUAE  Lysyl-tRNA synthetase (Lysine--tRNA lig...   110   1e-24
sp|Q4JCI5|SYK_SULAC  Lysyl-tRNA synthetase (Lysine--tRNA lig...   110   1e-24
>sp|Q6F2U9|SYK_ORYSA Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
          Length = 602

 Score =  138 bits (348), Expect = 5e-33
 Identities = 65/100 (65%), Positives = 82/100 (82%), Gaps = 3/100 (3%)
 Frame = +1

Query: 1   MYLRIAPELFLKELIIGGMNRVFEIGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITV 180
           +Y+RIAPEL+LKEL++GG++RV+EIGKQFRNE +DLTHNPEFTTCEFYMA+ADY DLI +
Sbjct: 321 LYMRIAPELYLKELVVGGLDRVYEIGKQFRNEGIDLTHNPEFTTCEFYMAYADYNDLIEL 380

Query: 181 TEDLLSNLVKELFGDYIVKYNRDG---KTYEIDFDPHTRE 291
           TE +LS +VKEL G Y +KY+ +G      EIDF P  R+
Sbjct: 381 TETMLSGMVKELTGGYKIKYHANGVEKPPIEIDFTPPFRK 420
>sp|Q9ZPI1|SYK_ARATH Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
          Length = 626

 Score =  138 bits (347), Expect = 6e-33
 Identities = 66/99 (66%), Positives = 80/99 (80%), Gaps = 3/99 (3%)
 Frame = +1

Query: 1   MYLRIAPELFLKELIIGGMNRVFEIGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITV 180
           +Y+RIAPEL+LK+LI+GG+ RV+EIGKQFRNE +DLTHNPEFTTCEFYMAFADY DL+ +
Sbjct: 330 LYMRIAPELYLKQLIVGGLERVYEIGKQFRNEGIDLTHNPEFTTCEFYMAFADYNDLMEM 389

Query: 181 TEDLLSNLVKELFGDYIVKYNRDG---KTYEIDFDPHTR 288
           TE +LS +VKEL G Y +KYN +G      EIDF P  R
Sbjct: 390 TEVMLSGMVKELTGGYKIKYNANGYDKDPIEIDFTPPFR 428
>sp|P37879|SYK_CRIGR Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
          Length = 597

 Score =  132 bits (333), Expect = 3e-31
 Identities = 60/99 (60%), Positives = 79/99 (79%), Gaps = 3/99 (3%)
 Frame = +1

Query: 1   MYLRIAPELFLKELIIGGMNRVFEIGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITV 180
           +Y+RIAPEL+ K L++GG++RV+EIG+QFRNE +DLTHNPEFTTCEFYMA+ADY DL+ +
Sbjct: 294 LYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEI 353

Query: 181 TEDLLSNLVKELFGDYIVKYNRD---GKTYEIDFDPHTR 288
           TE +LS +VK + G Y + Y+ D   G+ YEIDF P  R
Sbjct: 354 TEKMLSGMVKSITGSYKITYHPDGPEGEAYEIDFTPPFR 392
>sp|Q99MN1|SYK_MOUSE Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
          Length = 595

 Score =  132 bits (332), Expect = 4e-31
 Identities = 59/99 (59%), Positives = 79/99 (79%), Gaps = 3/99 (3%)
 Frame = +1

Query: 1   MYLRIAPELFLKELIIGGMNRVFEIGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITV 180
           +Y+RIAPEL+ K L++GG++RV+EIG+QFRNE +DLTHNPEFTTCEFYMA+ADY DL+ +
Sbjct: 292 LYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEI 351

Query: 181 TEDLLSNLVKELFGDYIVKYNRD---GKTYEIDFDPHTR 288
           TE +LS +VK + G Y + Y+ D   G+ YE+DF P  R
Sbjct: 352 TEKMLSGMVKSITGSYKITYHPDGPEGQAYEVDFTPPFR 390
>sp|Q15046|SYK_HUMAN Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
          Length = 597

 Score =  130 bits (327), Expect = 1e-30
 Identities = 58/99 (58%), Positives = 79/99 (79%), Gaps = 3/99 (3%)
 Frame = +1

Query: 1   MYLRIAPELFLKELIIGGMNRVFEIGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITV 180
           +Y+RIAPEL+ K L++GG++RV+EIG+QFRNE +DLTHNPEFTTCEFYMA+ADY DL+ +
Sbjct: 294 LYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEI 353

Query: 181 TEDLLSNLVKELFGDYIVKYNRD---GKTYEIDFDPHTR 288
           TE ++S +VK + G Y V Y+ D   G+ Y++DF P  R
Sbjct: 354 TEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFR 392
>sp|P15180|SYKC_YEAST Lysyl-tRNA synthetase, cytoplasmic (Lysine--tRNA ligase) (LysRS)
          Length = 591

 Score =  129 bits (323), Expect = 4e-30
 Identities = 57/95 (60%), Positives = 78/95 (82%), Gaps = 4/95 (4%)
 Frame = +1

Query: 1   MYLRIAPELFLKELIIGGMNRVFEIGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITV 180
           MY+RIAPELFLK+L++GG++RV+EIG+QFRNE +D+THNPEFTTCEFY A+AD +DL+ +
Sbjct: 297 MYMRIAPELFLKQLVVGGLDRVYEIGRQFRNEGIDMTHNPEFTTCEFYQAYADVYDLMDM 356

Query: 181 TEDLLSNLVKELFGDYIVKYNRD----GKTYEIDF 273
           TE + S +VKE+ G YI+KY+ D     K  E++F
Sbjct: 357 TELMFSEMVKEITGSYIIKYHPDPADPAKELELNF 391
>sp|Q22099|SYK_CAEEL Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
          Length = 572

 Score =  124 bits (312), Expect = 7e-29
 Identities = 55/97 (56%), Positives = 78/97 (80%), Gaps = 4/97 (4%)
 Frame = +1

Query: 1   MYLRIAPELFLKELIIGGMNRVFEIGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITV 180
           ++LR+APEL+ K L++GG++RV+E+G+ FRNE +DLTHNPEFTTCEFYMA+ADY D+I +
Sbjct: 273 LFLRVAPELYHKMLVVGGIDRVYEVGRLFRNEGIDLTHNPEFTTCEFYMAYADYEDVIQL 332

Query: 181 TEDLLSNLVKELFGDYIVKYNRDG----KTYEIDFDP 279
           TEDLLS++V  + G Y ++Y+ +G      YE+DF P
Sbjct: 333 TEDLLSSMVMSIKGTYKIEYHPNGPNTEPVYEVDFTP 369

 Score = 32.0 bits (71), Expect = 0.65
 Identities = 22/58 (37%), Positives = 29/58 (50%), Gaps = 4/58 (6%)
 Frame = +3

Query: 270 F*PPYQRIDMMTGL-EKLKMNCPSY*LVLKIS---FI**ICADNNVEWSAPRTTGSFI 431
           F PP++R+ M  GL EKL    P    +           +C DNNV+ SAPRTT   +
Sbjct: 367 FTPPFKRVHMYDGLAEKLGATLPDPSTLHTEEAREVFDKLCRDNNVDCSAPRTTARLL 424
>sp|Q43776|SYK_LYCES Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
          Length = 588

 Score =  121 bits (304), Expect = 6e-28
 Identities = 58/118 (49%), Positives = 85/118 (72%), Gaps = 3/118 (2%)
 Frame = +1

Query: 1   MYLRIAPELFLKELIIGGMNRVFEIGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITV 180
           + +R++PEL+LK+L++GG +RV+E+GK FRNE +DLTH+PEFT CE YMA+ADY DL+ +
Sbjct: 308 LLIRVSPELYLKKLVVGGFDRVYELGKHFRNEGMDLTHSPEFTMCELYMAYADYNDLMDL 367

Query: 181 TEDLLSNLVKELFGDYIVKYNRDG---KTYEIDFDPHTRESI**PVLRSLK*IALPTD 345
           TE LLS +VK+L G Y ++Y+ +G   +  EIDF P  R+      L  +  I++P D
Sbjct: 368 TEQLLSGMVKDLTGSYKIRYHANGLDNEPIEIDFTPPFRKIDMLSELEKVANISIPRD 425
>sp|O67258|SYK_AQUAE Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
          Length = 597

 Score =  110 bits (276), Expect = 1e-24
 Identities = 55/93 (59%), Positives = 66/93 (70%)
 Frame = +1

Query: 1   MYLRIAPELFLKELIIGGMNRVFEIGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITV 180
           +YLRIAPEL+LK LI+GG  RV+EIGK FRNESVD THNPEFT  EFY A+ DY DLI  
Sbjct: 320 LYLRIAPELYLKRLIVGGFPRVYEIGKNFRNESVDRTHNPEFTMVEFYAAYWDYHDLIKF 379

Query: 181 TEDLLSNLVKELFGDYIVKYNRDGKTYEIDFDP 279
           TED+   L+++  G   VKY      +E+DF P
Sbjct: 380 TEDMFVYLLEKTLGTLKVKYGE----WELDFSP 408
>sp|Q4JCI5|SYK_SULAC Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
          Length = 500

 Score =  110 bits (275), Expect = 1e-24
 Identities = 50/90 (55%), Positives = 68/90 (75%)
 Frame = +1

Query: 4   YLRIAPELFLKELIIGGMNRVFEIGKQFRNESVDLTHNPEFTTCEFYMAFADYFDLITVT 183
           YLRI+ EL+LK  I+GG N+VFEIGK FRNE +D+THNPEFT  E Y A+ADY D++ +T
Sbjct: 224 YLRISLELYLKRYIVGGFNKVFEIGKVFRNEDIDVTHNPEFTLLELYWAYADYNDIMRLT 283

Query: 184 EDLLSNLVKELFGDYIVKYNRDGKTYEIDF 273
           E++L ++VK +  D  +KY+  GK Y I+F
Sbjct: 284 EEMLQDVVKNINNDSKIKYSIGGKEYTIEF 313
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,169,134
Number of Sequences: 369166
Number of extensions: 1018385
Number of successful extensions: 3195
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3181
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2444192520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)