Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_M03
(577 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P28812|Y3568_PSEAE Hypothetical protein PA3568 140 2e-33
sp|O93730|ACSA_PYRAE Acetyl-coenzyme A synthetase (Acetate-... 98 1e-20
sp|Q99NB1|ACS2L_MOUSE Acetyl-coenzyme A synthetase 2-like, ... 95 1e-19
sp|P55912|PRPE_SALTY Propionate--CoA ligase (Propionyl-CoA ... 95 1e-19
sp|P77495|PRPE_ECOLI Propionate--CoA ligase (Propionyl-CoA ... 94 2e-19
sp|P36333|ACSA_PENCH Acetyl-coenzyme A synthetase (Acetate-... 92 9e-19
sp|P27095|ACSA_METSO Acetyl-coenzyme A synthetase (Acetate-... 92 1e-18
sp|Q55404|ACSA_SYNY3 Acetyl-coenzyme A synthetase (Acetate-... 91 2e-18
sp|Q01574|ACS1_YEAST Acetyl-coenzyme A synthetase 1 (Acetat... 91 3e-18
sp|Q9NUB1|ACS2L_HUMAN Acetyl-coenzyme A synthetase 2-like, ... 90 4e-18
>sp|P28812|Y3568_PSEAE Hypothetical protein PA3568
Length = 628
Score = 140 bits (353), Expect = 2e-33
Identities = 68/146 (46%), Positives = 99/146 (67%)
Frame = +3
Query: 3 YYDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQL 182
YY T D G + E G + +M R DDV+N++GHR+S+G +EE ++ V ECAVIG+ D+L
Sbjct: 473 YYHTGDGGYLDEDGFVYIMGRTDDVINVSGHRLSTGEMEERVAQHQAVAECAVIGVRDEL 532
Query: 183 KGQVPLALLVPDTDTIKAPEEIVSDTIKIVRDRIGAVAGMRNAVVVHRLPKTRSGKISRN 362
KG VPL L+V D A E++ + + +VR++IGA+A + VVV RLPKTRSGKI R
Sbjct: 533 KGHVPLGLVVLKDDAGIAAEQLQRELVALVREQIGALACFQRVVVVKRLPKTRSGKILRA 592
Query: 363 AIAAMANGKPFKAPVTLEDPTVFDPI 440
+ +A+G+ + AP T++DPT+ I
Sbjct: 593 VLRKIADGEDYAAPSTIDDPTILGEI 618
>sp|O93730|ACSA_PYRAE Acetyl-coenzyme A synthetase (Acetate--CoA ligase) (Acyl-activating
enzyme)
Length = 670
Score = 98.2 bits (243), Expect = 1e-20
Identities = 54/140 (38%), Positives = 81/140 (57%)
Frame = +3
Query: 42 GHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLKGQVPLALLVPDT 221
G+I V+ R D+V+ +AGHR+ + LE A + +P V E AV+G+ D +KG+VP+A +V
Sbjct: 525 GYIWVLGRADEVIKVAGHRLGTYELESALISHPAVAESAVVGVPDAIKGEVPIAFVVL-K 583
Query: 222 DTIKAPEEIVSDTIKIVRDRIGAVAGMRNAVVVHRLPKTRSGKISRNAIAAMANGKPFKA 401
+ +E+ + + VR IG +A V +LPKTRSGKI R + A+A G P
Sbjct: 584 QGVAPSDELRKELREHVRRTIGPIAEPAQIFFVTKLPKTRSGKIMRRLLKAVATGAPLGD 643
Query: 402 PVTLEDPTVFDPIYNAFIQI 461
TLED T + A+ +I
Sbjct: 644 VTTLEDETSVEEAKRAYEEI 663
>sp|Q99NB1|ACS2L_MOUSE Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor
(Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2)
(AceCS2) (Acyl-CoA synthetase short-chain family member
1)
Length = 682
Score = 95.1 bits (235), Expect = 1e-19
Identities = 57/152 (37%), Positives = 82/152 (53%), Gaps = 2/152 (1%)
Frame = +3
Query: 3 YYDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQL 182
YY T D TE G+ + R DDV+NI+GHR+ + +E+A ++P V E AVIG +
Sbjct: 521 YYFTGDGAHRTEGGYYQITGRMDDVINISGHRLGTAEIEDAMADHPAVPETAVIGYPHDI 580
Query: 183 KGQVPLALLVPDTDTIKAPEEIVSDTIKIVRDRIGAVAGMRNAVVVHRLPKTRSGKISRN 362
KG+ A +V D I +V++ V +I A +VV RLPKTRSGK+ R
Sbjct: 581 KGEAAFAFIVL-KDNISDENMVVNELKLSVATKIAKYAVPDQILVVKRLPKTRSGKVMRR 639
Query: 363 AIAAM--ANGKPFKAPVTLEDPTVFDPIYNAF 452
+ + + G+ TLEDP+V I +AF
Sbjct: 640 LLRKIITSRGQDLGDTTTLEDPSVITEILSAF 671
>sp|P55912|PRPE_SALTY Propionate--CoA ligase (Propionyl-CoA synthetase)
Length = 628
Score = 94.7 bits (234), Expect = 1e-19
Identities = 55/152 (36%), Positives = 80/152 (52%), Gaps = 4/152 (2%)
Frame = +3
Query: 6 YDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLK 185
Y T D G+ G+ ++ R DDV+NIAGHR+ + +EE+ P V E AV+G+ D LK
Sbjct: 474 YATFDWGIRDAEGYYFILGRTDDVINIAGHRLGTREIEESISSYPNVAEVAVVGIKDALK 533
Query: 186 GQVPLALLVPDTDTIKAPEEIVSD----TIKIVRDRIGAVAGMRNAVVVHRLPKTRSGKI 353
GQV +A ++P A E D + +V ++IG + V +LPKTRSGK+
Sbjct: 534 GQVAVAFVIPKQSDTLADREAARDEENAIMALVDNQIGHFGRPAHVWFVSQLPKTRSGKM 593
Query: 354 SRNAIAAMANGKPFKAPVTLEDPTVFDPIYNA 449
R I A+ G+ T++DP I A
Sbjct: 594 LRRTIQAICEGRDPGDLTTIDDPASLQQIRQA 625
>sp|P77495|PRPE_ECOLI Propionate--CoA ligase (Propionyl-CoA synthetase)
Length = 628
Score = 94.4 bits (233), Expect = 2e-19
Identities = 55/155 (35%), Positives = 84/155 (54%), Gaps = 7/155 (4%)
Frame = +3
Query: 6 YDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLK 185
Y T D G+ G+ ++ R DDV+N+AGHR+ + +EE+ +P V E AV+G+ D LK
Sbjct: 474 YATFDWGIRDADGYHFILGRTDDVINVAGHRLGTREIEESISSHPGVAEVAVVGVKDALK 533
Query: 186 GQVPLALLVP-------DTDTIKAPEEIVSDTIKIVRDRIGAVAGMRNAVVVHRLPKTRS 344
GQV +A ++P D D + E+ + + +V +IG + V +LPKTRS
Sbjct: 534 GQVAVAFVIPKESDSLEDRDVAHSQEKAI---MALVDSQIGNFGRPAHVWFVSQLPKTRS 590
Query: 345 GKISRNAIAAMANGKPFKAPVTLEDPTVFDPIYNA 449
GK+ R I A+ G+ T++DP D I A
Sbjct: 591 GKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQA 625
>sp|P36333|ACSA_PENCH Acetyl-coenzyme A synthetase (Acetate--CoA ligase) (Acyl-activating
enzyme)
Length = 669
Score = 92.0 bits (227), Expect = 9e-19
Identities = 58/148 (39%), Positives = 78/148 (52%), Gaps = 2/148 (1%)
Frame = +3
Query: 3 YYDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQL 182
YY T D G+ + R DDV+N++GHR+S+ +E A LE+P V E AV+G+ D+L
Sbjct: 514 YYFTGDGAGRDHDGYYWIRGRVDDVVNVSGHRLSTAEIEAALLEHPSVAEAAVVGIADEL 573
Query: 183 KGQVPLALLVPDTDTIKAPEEIVSDTIKIVRDRIGAVAGMRNAVVVHRLPKTRSGKISRN 362
GQ A + K E+I D VR IG A + VV LPKTRSGKI R
Sbjct: 574 TGQAVNAFV--SLKEGKPTEQISKDLAMQVRKSIGPFAAPKAVFVVDDLPKTRSGKIMRR 631
Query: 363 AIAAMANGK--PFKAPVTLEDPTVFDPI 440
+ + +G+ TL DP+V D I
Sbjct: 632 ILRKILSGEEDSLGDTSTLSDPSVVDKI 659
>sp|P27095|ACSA_METSO Acetyl-coenzyme A synthetase (Acetate--CoA ligase) (Acyl-activating
enzyme)
Length = 672
Score = 91.7 bits (226), Expect = 1e-18
Identities = 51/133 (38%), Positives = 74/133 (55%)
Frame = +3
Query: 42 GHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLKGQVPLALLVPDT 221
G+ + R DDVL++AGHRI++ +E A + +P + E AV+G D++KG+ +A ++
Sbjct: 537 GYFFIQGRIDDVLSVAGHRIANAEVESALVAHPKIAEAAVVGKPDEVKGESIVAFVILRV 596
Query: 222 DTIKAPEEIVSDTIKIVRDRIGAVAGMRNAVVVHRLPKTRSGKISRNAIAAMANGKPFKA 401
+P E+ D I VR +G VA V+ LPKTRSGKI R + A A G P
Sbjct: 597 GNEPSP-ELAKDAIAFVRKTLGPVAAPTEVHFVNDLPKTRSGKIMRRVVKARALGNPVGD 655
Query: 402 PVTLEDPTVFDPI 440
TL +P D I
Sbjct: 656 ISTLMNPEAVDGI 668
>sp|Q55404|ACSA_SYNY3 Acetyl-coenzyme A synthetase (Acetate--CoA ligase) (Acyl-activating
enzyme)
Length = 653
Score = 91.3 bits (225), Expect = 2e-18
Identities = 51/134 (38%), Positives = 77/134 (57%), Gaps = 1/134 (0%)
Frame = +3
Query: 42 GHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQLKGQVPLALLVPDT 221
G+ VM R DDV+N++GHR+ + +E A + +P V E AV+G D+L G+ A + +
Sbjct: 518 GYFWVMGRVDDVINVSGHRLGTMEIESALVSHPLVAEAAVVGRPDELTGEAIFAFVSLEG 577
Query: 222 DTIKAPEEIVSDTIKIVRDRIGAVAGMRNAVVVHRLPKTRSGKISRNAIAAMANGKPFKA 401
+ + EE+ D +K V + IGA+A LPKTRSGKI R + ++A+G+
Sbjct: 578 NA-EPSEELKKDLVKHVTEEIGAIARPAEIRFTDVLPKTRSGKIMRRLLRSLASGQEISG 636
Query: 402 PV-TLEDPTVFDPI 440
TLED TV D +
Sbjct: 637 DTSTLEDRTVLDKL 650
>sp|Q01574|ACS1_YEAST Acetyl-coenzyme A synthetase 1 (Acetate--CoA ligase 1)
(Acyl-activating enzyme 1)
Length = 713
Score = 90.5 bits (223), Expect = 3e-18
Identities = 51/135 (37%), Positives = 75/135 (55%), Gaps = 6/135 (4%)
Frame = +3
Query: 3 YYDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQL 182
YY T D + G+I ++ R DDV+N++GHR+S+ +E A +E+P V ECAV+G +D L
Sbjct: 554 YYFTGDGAAKDKDGYIWILGRVDDVVNVSGHRLSTAEIEAAIIEDPIVAECAVVGFNDDL 613
Query: 183 KGQVPLALLVP------DTDTIKAPEEIVSDTIKIVRDRIGAVAGMRNAVVVHRLPKTRS 344
GQ A +V T T ++I + VR IG A + ++V LPKTRS
Sbjct: 614 TGQAVAAFVVLKNKSSWSTATDDELQDIKKHLVFTVRKDIGPFAAPKLIILVDDLPKTRS 673
Query: 345 GKISRNAIAAMANGK 389
GKI R + + G+
Sbjct: 674 GKIMRRILRKILAGE 688
>sp|Q9NUB1|ACS2L_HUMAN Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor
(Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2)
(Acyl-CoA synthetase short-chain family member 1)
Length = 689
Score = 90.1 bits (222), Expect = 4e-18
Identities = 52/152 (34%), Positives = 81/152 (53%), Gaps = 2/152 (1%)
Frame = +3
Query: 3 YYDTMDAGMVTEFGHIMVMARDDDVLNIAGHRISSGALEEACLENPCVVECAVIGLDDQL 182
YY T D TE G+ + R DDV+NI+GHR+ + +E+A ++P V E AVIG +
Sbjct: 528 YYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDI 587
Query: 183 KGQVPLALLVPDTDTIKAPEEIVSDTIKIVRDRIGAVAGMRNAVVVHRLPKTRSGKISRN 362
KG+ A +V D+ + +V + +V +I A +VV RLPKTRSGK+ R
Sbjct: 588 KGEAAFAFIVV-KDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRR 646
Query: 363 AIAAM--ANGKPFKAPVTLEDPTVFDPIYNAF 452
+ + + + TLEDP++ I + +
Sbjct: 647 LLRKIITSEAQELGDTTTLEDPSIIAEILSVY 678
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,119,308
Number of Sequences: 369166
Number of extensions: 1230318
Number of successful extensions: 3423
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3330
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4275400980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)