Planarian EST Database


Dr_sW_014_L23

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_014_L23
         (622 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q01241|NF70_LOLPE  70 kDa neurofilament protein (NF70)          96   1e-19
sp|Q01240|NF60_LOLPE  60 kDa neurofilament protein (NF60)          96   1e-19
sp|P90901|IFA1_CAEEL  Intermediate filament protein ifa-1 (I...    80   3e-15
sp|P31732|OV71_ONCVO  Muscle cell intermediate filament prot...    80   4e-15
sp|Q17107|AV71_ACAVI  Muscle cell intermediate filament prot...    79   9e-15
sp|P23731|IFEB_ASCSU  Intermediate filament protein B (IF-B)       71   2e-12
sp|O02365|IFA2_CAEEL  Intermediate filament protein ifa-2 (I...    70   4e-12
sp|Q19289|IFB1_CAEEL  Intermediate filament protein ifb-1 (I...    69   1e-11
sp|P90900|IFA4_CAEEL  Intermediate filament protein ifa-4 (I...    67   5e-11
sp|Q21065|IFA3_CAEEL  Intermediate filament protein ifa-3 (I...    66   6e-11
>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
          Length = 615

 Score = 95.5 bits (236), Expect = 1e-19
 Identities = 53/118 (44%), Positives = 71/118 (60%)
 Frame = +3

Query: 3   QMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEE 182
           ++QE RT  T+ N+E T  KEEN+++KS +  +R+R+ADLE +NA+ E     +  + EE
Sbjct: 332 KVQEFRTGATKQNMEVTRNKEENTKLKSNMTEIRNRLADLEARNAQLERTNQDLLRDLEE 391

Query: 183 TQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLEFEESRV 356
             R  EL+                + L ELQ L+D KL LELEIAAY KLLE EESRV
Sbjct: 392 KDRQNELESCQYKEEITKLRGEMESILKELQDLMDIKLSLELEIAAYRKLLEGEESRV 449
>sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60)
          Length = 511

 Score = 95.5 bits (236), Expect = 1e-19
 Identities = 53/118 (44%), Positives = 71/118 (60%)
 Frame = +3

Query: 3   QMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEE 182
           ++QE RT  T+ N+E T  KEEN+++KS +  +R+R+ADLE +NA+ E     +  + EE
Sbjct: 332 KVQEFRTGATKQNMEVTRNKEENTKLKSNMTEIRNRLADLEARNAQLERTNQDLLRDLEE 391

Query: 183 TQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLEFEESRV 356
             R  EL+                + L ELQ L+D KL LELEIAAY KLLE EESRV
Sbjct: 392 KDRQNELESCQYKEEITKLRGEMESILKELQDLMDIKLSLELEIAAYRKLLEGEESRV 449
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
           A1) (IF-A1) (Cel IF A1)
          Length = 575

 Score = 80.5 bits (197), Expect = 3e-15
 Identities = 44/117 (37%), Positives = 65/117 (55%)
 Frame = +3

Query: 3   QMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEE 182
           ++QEI+T   R N+E  + KEE  R+++Q+  +R ++ADLE +N+  E  +  +    E+
Sbjct: 305 KVQEIQTQSARQNMEQGYAKEEVKRLRTQLSDLRGKLADLESRNSLLEKQIQELNYQLED 364

Query: 183 TQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLEFEESR 353
            QRS E  L               A + ELQ LLD K  L+ EIA Y K+LE EE+R
Sbjct: 365 DQRSYEAALNDRDSQIRKMREECQALMVELQMLLDTKQTLDAEIAIYRKMLEGEENR 421
>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
          Length = 432

 Score = 80.1 bits (196), Expect = 4e-15
 Identities = 45/117 (38%), Positives = 65/117 (55%)
 Frame = +3

Query: 3   QMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEE 182
           ++QEI+T  TR N+E  + KEE  R++ Q+  +R ++ADLE +N+  E  +  +    E+
Sbjct: 160 KVQEIQTQSTRQNLEQGYAKEEVKRLRVQLSDLRGKLADLEGRNSLLEKQMQELNYQLED 219

Query: 183 TQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLEFEESR 353
            QRS E  L               A + ELQ LLD K  L+ EIA Y K+LE EE+R
Sbjct: 220 DQRSYEAALNDRDAQIRKMREECQALMMELQMLLDTKQTLDAEIAIYRKMLEGEENR 276
>sp|Q17107|AV71_ACAVI Muscle cell intermediate filament protein AV71
          Length = 394

 Score = 79.0 bits (193), Expect = 9e-15
 Identities = 45/117 (38%), Positives = 64/117 (54%)
 Frame = +3

Query: 3   QMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEE 182
           ++QEI+T  TR N+E  + KEE  R++ Q+  +R ++ADLE +N+  E     +    E+
Sbjct: 122 KVQEIQTQSTRQNLEQGYAKEEVKRLRVQLSELRGKLADLEGRNSLLEKQTQELNYQLED 181

Query: 183 TQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLEFEESR 353
            QRS E  L               A + ELQ LLD K  L+ EIA Y K+LE EE+R
Sbjct: 182 DQRSYEAALNDRDAQIRKMREECQALMMELQMLLDTKQTLDAEIAIYRKMLEGEENR 238
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
          Length = 589

 Score = 71.2 bits (173), Expect = 2e-12
 Identities = 40/118 (33%), Positives = 62/118 (52%)
 Frame = +3

Query: 3   QMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEE 182
           ++ E++ S  R N+E+++ +EE  R++  I  +R ++ DLE +NA  E  +  +     +
Sbjct: 316 KVSEVQGSANRANMESSYQREEVKRMRDNIGDLRGKLGDLEAKNALLEKEVQNLNYQLND 375

Query: 183 TQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLEFEESRV 356
            QR  E  L                 + ELQ+LLD K  L+ EIA Y K+LE EESRV
Sbjct: 376 DQRQYEAALNDRDATLRRMREECQTLVAELQALLDTKQMLDAEIAIYRKMLEGEESRV 433
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
           A2) (IF-A2) (Cel IF A2)
          Length = 581

 Score = 70.1 bits (170), Expect = 4e-12
 Identities = 41/125 (32%), Positives = 61/125 (48%)
 Frame = +3

Query: 3   QMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEE 182
           ++QEI T   R N E  + KEE  R+++Q   +R +++DLE +N   E  +  +    E+
Sbjct: 307 RVQEINTQSNRQNAENNYQKEEVKRLRNQTSELRQKLSDLESRNLLLEKQIEDLNYQLED 366

Query: 183 TQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLEFEESRVNS 362
            QRS E  L               A + ELQ LLD K  L+ E+  Y ++LE        
Sbjct: 367 DQRSYEAALNDKDAQIRKLREECQALMVELQMLLDTKQTLDGELKVYRQMLE-------G 419

Query: 363 NTEGN 377
           N+EGN
Sbjct: 420 NSEGN 424
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
           B1) (IF-B1) (Cel IF B1)
          Length = 589

 Score = 68.6 bits (166), Expect = 1e-11
 Identities = 38/118 (32%), Positives = 62/118 (52%)
 Frame = +3

Query: 3   QMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEE 182
           ++ E++ S  R N+E+T+ ++E  R++  I  +R ++ DLE +N+  E  +  +     +
Sbjct: 316 KVSEVQGSANRANMESTYQRDEVKRMRDNIGDLRGKLGDLENKNSLLEKEVQNLNYQLTD 375

Query: 183 TQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLEFEESRV 356
            QR  E  L                 + ELQ+LLD K  L+ EIA Y K+LE EE+RV
Sbjct: 376 DQRQYEAALNDRDATLRRMREECQTLVAELQALLDTKQMLDAEIAIYRKMLEGEETRV 433
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
           A4) (IF-A4) (Cel IF A4)
          Length = 575

 Score = 66.6 bits (161), Expect = 5e-11
 Identities = 38/117 (32%), Positives = 60/117 (51%)
 Frame = +3

Query: 3   QMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEE 182
           ++QEI T  +R+ +E  + +EE  R+++ +  MR ++ADLE +N   E  +  +    EE
Sbjct: 306 KVQEIHTQNSRNCLEQNYAREEVKRLRTTLGDMRGKMADLEGRNLLLEKQIEDLNYQMEE 365

Query: 183 TQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLEFEESR 353
             RS E  L                 + ELQ L+D K  L+ EI  Y K+L+ EE+R
Sbjct: 366 DMRSYEQSLNDKDTSINKLRDESKILMVELQMLIDTKQTLDAEIVIYRKMLDGEENR 422

 Score = 35.4 bits (80), Expect = 0.12
 Identities = 22/63 (34%), Positives = 36/63 (57%)
 Frame = +3

Query: 15  IRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEETQRS 194
           IR S+TR+  E +   E N R+ S I+ +R     LE QN + E +LN++RG +     S
Sbjct: 63  IRESRTREKKEMS---ELNDRLASYIEKVRF----LEAQNRKMEKDLNLLRGKWGHDSTS 115

Query: 195 LEL 203
           +++
Sbjct: 116 VKV 118
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
           A3) (IF-A3) (Cel IF A3)
          Length = 581

 Score = 66.2 bits (160), Expect = 6e-11
 Identities = 38/112 (33%), Positives = 55/112 (49%)
 Frame = +3

Query: 3   QMQEIRTSKTRDNVETTHYKEENSRIKSQIQSMRSRIADLEMQNAEYEHNLNMIRGNYEE 182
           ++QEI T   R N E  H ++E  R+ SQ+  ++S+ A+L  +N   E  L  +    E+
Sbjct: 307 RVQEINTQTNRQNAEINHKRDEVKRLHSQVSELKSKHAELAARNGLLEKQLEDLNYQLED 366

Query: 183 TQRSLELKLMSXXXXXXXXXXXXSAALTELQSLLDAKLGLELEIAAYAKLLE 338
            QRS E  L               A L ELQ LLD K  L+ E+  Y ++LE
Sbjct: 367 DQRSYEAALNDKDAQVRKLREECQALLVELQMLLDTKQTLDGELKVYRRMLE 418
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,395,356
Number of Sequences: 369166
Number of extensions: 440715
Number of successful extensions: 1782
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1580
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1738
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4877307000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)