Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_L16
(424 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O14427|CLA4_CANAL Serine/threonine-protein kinase CLA4 45 6e-05
sp|P22216|RAD53_YEAST Serine/threonine-protein kinase RAD53... 43 3e-04
sp|Q39030|KPK19_ARATH Serine/threonine-protein kinase AtPK1... 42 5e-04
sp|P42818|KPK1_ARATH Serine/threonine-protein kinase AtPK1/... 42 7e-04
sp|P48562|CLA4_YEAST Serine/threonine-protein kinase CLA4 41 0.001
sp|P49025|CTRO_MOUSE Citron Rho-interacting kinase (CRIK) (... 41 0.001
sp|Q91ZR4|NEK8_MOUSE Serine/threonine-protein kinase Nek8 (... 41 0.001
sp|Q86SG6|NEK8_HUMAN Serine/threonine-protein kinase Nek8 (... 41 0.001
sp|Q9HFW2|CLA4_ASHGO Serine/threonine-protein kinase CLA4 41 0.001
sp|Q9BXA7|TSSK1_HUMAN Testis-specific serine/threonine-prot... 40 0.002
>sp|O14427|CLA4_CANAL Serine/threonine-protein kinase CLA4
Length = 971
Score = 45.1 bits (105), Expect = 6e-05
Identities = 31/75 (41%), Positives = 40/75 (53%)
Frame = +1
Query: 175 LDTINELHKLGIIHRDIKSDNIVIDDTIMQN**FAITIYTLIDK*RSNR*TGSI*PGTIC 354
L + LHK IIHRDIKSDN+++D N IT + K R + GT
Sbjct: 788 LKGLQHLHKKHIIHRDIKSDNVLLD--AYGN--VKITDFGFCAKLTDQRNKRATMVGTPY 843
Query: 355 YMAPELIKGKLYSEK 399
+MAPE++K K Y EK
Sbjct: 844 WMAPEVVKQKEYDEK 858
>sp|P22216|RAD53_YEAST Serine/threonine-protein kinase RAD53 (Serine-protein kinase 1)
Length = 821
Score = 42.7 bits (99), Expect = 3e-04
Identities = 29/72 (40%), Positives = 41/72 (56%), Gaps = 2/72 (2%)
Frame = +1
Query: 175 LDTINELHKLGIIHRDIKSDNIVI--DDTIMQN**FAITIYTLIDK*RSNR*TGSI*PGT 348
L I +H +GI HRD+K DNI+I DD ++ IT + L K + N GT
Sbjct: 304 LTAIKYIHSMGISHRDLKPDNILIEQDDPVL----VKITDFGLA-KVQGNGSFMKTFCGT 358
Query: 349 ICYMAPELIKGK 384
+ Y+APE+I+GK
Sbjct: 359 LAYVAPEVIRGK 370
>sp|Q39030|KPK19_ARATH Serine/threonine-protein kinase AtPK19 (Ribosomal-protein S6 kinase
homolog)
Length = 471
Score = 42.0 bits (97), Expect = 5e-04
Identities = 25/72 (34%), Positives = 43/72 (59%), Gaps = 2/72 (2%)
Frame = +1
Query: 175 LDTINELHKLGIIHRDIKSDNIV--IDDTIMQN**FAITIYTLIDK*RSNR*TGSI*PGT 348
+ ++ LH+ GI+HRD+K +NI+ +D +M +T + L + N + S+ GT
Sbjct: 248 VSAVSHLHEKGIMHRDLKPENILMDVDGHVM------LTDFGLAKEFEENTRSNSM-CGT 300
Query: 349 ICYMAPELIKGK 384
YMAPE+++GK
Sbjct: 301 TEYMAPEIVRGK 312
>sp|P42818|KPK1_ARATH Serine/threonine-protein kinase AtPK1/AtPK6
Length = 465
Score = 41.6 bits (96), Expect = 7e-04
Identities = 25/72 (34%), Positives = 43/72 (59%), Gaps = 2/72 (2%)
Frame = +1
Query: 175 LDTINELHKLGIIHRDIKSDNIVIDDT--IMQN**FAITIYTLIDK*RSNR*TGSI*PGT 348
+ ++ LH+ GI+HRD+K +NI++D +M +T + L + N + S+ GT
Sbjct: 242 VSAVSHLHEKGIMHRDLKPENILMDTDGHVM------LTDFGLAKEFEENTRSNSM-CGT 294
Query: 349 ICYMAPELIKGK 384
YMAPE+++GK
Sbjct: 295 TEYMAPEIVRGK 306
>sp|P48562|CLA4_YEAST Serine/threonine-protein kinase CLA4
Length = 842
Score = 41.2 bits (95), Expect = 0.001
Identities = 26/69 (37%), Positives = 36/69 (52%)
Frame = +1
Query: 193 LHKLGIIHRDIKSDNIVIDDTIMQN**FAITIYTLIDK*RSNR*TGSI*PGTICYMAPEL 372
LH IIHRDIKSDN+++D IT + + R + GT +MAPE+
Sbjct: 684 LHDKHIIHRDIKSDNVLLDTRAR----VKITDFGFCARLTDKRSKRATMVGTPYWMAPEV 739
Query: 373 IKGKLYSEK 399
+K + Y EK
Sbjct: 740 VKQREYDEK 748
>sp|P49025|CTRO_MOUSE Citron Rho-interacting kinase (CRIK) (Rho-interacting,
serine/threonine-protein kinase 21)
Length = 2055
Score = 41.2 bits (95), Expect = 0.001
Identities = 24/76 (31%), Positives = 40/76 (52%)
Frame = +1
Query: 184 INELHKLGIIHRDIKSDNIVIDDTIMQN**FAITIYTLIDK*RSNR*TGSI*PGTICYMA 363
++ +H++G +HRDIK +NI+ID T + + K SN+ + GT YMA
Sbjct: 209 VHSVHQMGYVHRDIKPENILIDRTGE----IKLVDFGSAAKMNSNKVDAKLPIGTPDYMA 264
Query: 364 PELIKGKLYSEKSTFG 411
PE++ + T+G
Sbjct: 265 PEVLTVMNEDRRGTYG 280
>sp|Q91ZR4|NEK8_MOUSE Serine/threonine-protein kinase Nek8 (NimA-related protein kinase
8)
Length = 698
Score = 40.8 bits (94), Expect = 0.001
Identities = 26/74 (35%), Positives = 42/74 (56%), Gaps = 1/74 (1%)
Frame = +1
Query: 184 INELHKLGIIHRDIKSDNIVIDDTIMQN**FAITIYTL-IDK*RSNR*TGSI*PGTICYM 360
++ +H I+HRD+K+ NI++D M + I I K S++ GT CY+
Sbjct: 116 LHHVHTHLILHRDLKTQNILLDKHRM-----VVKIGDFGISKILSSKSKAYTVVGTPCYI 170
Query: 361 APELIKGKLYSEKS 402
+PEL +GK Y++KS
Sbjct: 171 SPELCEGKPYNQKS 184
>sp|Q86SG6|NEK8_HUMAN Serine/threonine-protein kinase Nek8 (NimA-related protein kinase
8) (NIMA-related kinase 12a)
Length = 692
Score = 40.8 bits (94), Expect = 0.001
Identities = 26/74 (35%), Positives = 42/74 (56%), Gaps = 1/74 (1%)
Frame = +1
Query: 184 INELHKLGIIHRDIKSDNIVIDDTIMQN**FAITIYTL-IDK*RSNR*TGSI*PGTICYM 360
++ +H I+HRD+K+ NI++D M + I I K S++ GT CY+
Sbjct: 116 LHHVHTHLILHRDLKTQNILLDKHRM-----VVKIGDFGISKILSSKSKAYTVVGTPCYI 170
Query: 361 APELIKGKLYSEKS 402
+PEL +GK Y++KS
Sbjct: 171 SPELCEGKPYNQKS 184
>sp|Q9HFW2|CLA4_ASHGO Serine/threonine-protein kinase CLA4
Length = 793
Score = 40.8 bits (94), Expect = 0.001
Identities = 27/69 (39%), Positives = 37/69 (53%)
Frame = +1
Query: 193 LHKLGIIHRDIKSDNIVIDDTIMQN**FAITIYTLIDK*RSNR*TGSI*PGTICYMAPEL 372
LH IIHRDIKSDN+++D + IT + K R + GT +MAPE+
Sbjct: 635 LHDKHIIHRDIKSDNVLLD----THGRVKITDFGFCAKLTDKRSKRATMVGTPYWMAPEV 690
Query: 373 IKGKLYSEK 399
+K + Y EK
Sbjct: 691 VKQREYDEK 699
>sp|Q9BXA7|TSSK1_HUMAN Testis-specific serine/threonine-protein kinase 1 (TSSK-1)
(Testis-specific kinase 1) (TSK-1)
(Serine/threonine-protein kinase 22A)
Length = 367
Score = 40.4 bits (93), Expect = 0.002
Identities = 31/100 (31%), Positives = 49/100 (49%), Gaps = 1/100 (1%)
Frame = +1
Query: 103 DLSDLIWTEENIYRNEK**KFHMDLDTINELHKLGIIHRDIKSDNIVID-DTIMQN**FA 279
DL +LI T ++ +E KFH I H L ++HRD+K DN+++D D ++ F+
Sbjct: 97 DLLELIKTRGALHEDEARKKFHQLSLAIKYCHDLDVVHRDLKCDNLLLDKDFNIKLSDFS 156
Query: 280 ITIYTLIDK*RSNR*TGSI*PGTICYMAPELIKGKLYSEK 399
+ L D G+ Y APE+++G Y K
Sbjct: 157 FSKRCLRDD-SGRMALSKTFCGSPAYAAPEVLQGIPYQPK 195
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,792,654
Number of Sequences: 369166
Number of extensions: 805217
Number of successful extensions: 3739
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3224
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3549
length of database: 68,354,980
effective HSP length: 99
effective length of database: 50,066,215
effective search space used: 2052714815
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)