Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_K03
(513 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9U4L6|TOM40_DROME Probable mitochondrial import recepto... 88 1e-17
sp|Q9QYA2|TOM40_MOUSE Probable mitochondrial import recepto... 81 2e-15
sp|O96008|TOM40_HUMAN Probable mitochondrial import recepto... 77 2e-14
sp|Q18090|TOM40_CAEEL Probable mitochondrial import recepto... 72 6e-13
sp|O13656|TOM40_SCHPO Probable mitochondrial import recepto... 42 7e-04
sp|P19812|UBR1_YEAST Ubiquitin-protein ligase E3 component ... 31 1.5
sp|P27571|XIST_MOUSE X inactive-specific transcript protein 30 4.4
sp|P61872|LIP_RHIOR Lipase precursor (Triacylglycerol lipas... 28 9.7
>sp|Q9U4L6|TOM40_DROME Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
(Male sterile protein 15)
Length = 344
Score = 88.2 bits (217), Expect = 1e-17
Identities = 48/147 (32%), Positives = 79/147 (53%), Gaps = 2/147 (1%)
Frame = +3
Query: 24 MRRITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNK--WTAAVGVRPFQSSLHSTF 197
++ +T L+LGS +Q + +V G+Y W+ +G QS LH +
Sbjct: 202 LQSVTPALALGSELAYQFGPNVPGRQIAIMSVVGRYTAGSSVWSGTLG----QSGLHVCY 257
Query: 198 HMKLNEAFQIGTEIETSLGHRQCVGTAGYQFDFPKSGITIKSQVDSNWKIATSYERKFIA 377
+ K ++ QIG E+ETSL ++ V T YQ D PK+ + + +DSNW+I E++ +A
Sbjct: 258 YQKASDQLQIGAEVETSLRMQESVATLAYQIDLPKANLVFRGGIDSNWQIFGVLEKR-LA 316
Query: 378 PIPFSINLCINGDIWKSNYGLGVGFSI 458
P+PF++ L + K+N+ LG G I
Sbjct: 317 PLPFTLALSGRMNHVKNNFRLGCGLMI 343
>sp|Q9QYA2|TOM40_MOUSE Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
Length = 359
Score = 80.9 bits (198), Expect = 2e-15
Identities = 45/145 (31%), Positives = 78/145 (53%)
Frame = +3
Query: 24 MRRITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNKWTAAVGVRPFQSSLHSTFHM 203
++ IT L+LG V+ R + T V ++ G+Y N W A V + Q+ +H+T++
Sbjct: 220 LQSITPCLALGGELVYHRRPGEEGT---VMSLAGKYTLNNWLATVTLG--QAGMHATYYH 274
Query: 204 KLNEAFQIGTEIETSLGHRQCVGTAGYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPI 383
K ++ Q+G E E S + + GYQ D PK+ K V+SNW + + E+K + P+
Sbjct: 275 KASDQLQVGVEFEASTRMQDTSASFGYQLDLPKANFLFKGSVNSNWIVGATLEKK-LPPL 333
Query: 384 PFSINLCINGDIWKSNYGLGVGFSI 458
P +++LC + K+ + G G +I
Sbjct: 334 PLTLSLCAFLNHRKNKFLCGFGLTI 358
>sp|O96008|TOM40_HUMAN Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
(Haymaker protein) (p38.5)
Length = 361
Score = 77.4 bits (189), Expect = 2e-14
Identities = 45/145 (31%), Positives = 77/145 (53%)
Frame = +3
Query: 24 MRRITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNKWTAAVGVRPFQSSLHSTFHM 203
++ IT L+LG V+ R + T V ++ G+Y N W A V + Q+ +H+T++
Sbjct: 222 LQSITPCLALGGELVYHRRPGEEGT---VMSLAGKYTLNNWLATVTLG--QAGMHATYYH 276
Query: 204 KLNEAFQIGTEIETSLGHRQCVGTAGYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPI 383
K ++ Q+G E E S + + GYQ D PK+ + K VDSNW + + E+K + P+
Sbjct: 277 KASDQLQVGVEFEASTRMQDTSVSFGYQLDLPKANLLFKGSVDSNWIVGATLEKK-LPPL 335
Query: 384 PFSINLCINGDIWKSNYGLGVGFSI 458
P ++ L + K+ + G G +I
Sbjct: 336 PLTLALGAFLNHRKNKFQCGFGLTI 360
>sp|Q18090|TOM40_CAEEL Probable mitochondrial import receptor subunit TOM40 homolog
(Translocase of outer membrane 40 kDa subunit homolog)
Length = 301
Score = 72.4 bits (176), Expect = 6e-13
Identities = 39/145 (26%), Positives = 71/145 (48%)
Frame = +3
Query: 24 MRRITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNKWTAAVGVRPFQSSLHSTFHM 203
+RR+T +L +G+ V+Q V + +Y N + AA + S +H T++
Sbjct: 158 LRRLTPRLDVGTEMVYQYGKNIPGGQISVLSYAARYTANHFIAAATLGA--SGVHLTYYH 215
Query: 204 KLNEAFQIGTEIETSLGHRQCVGTAGYQFDFPKSGITIKSQVDSNWKIATSYERKFIAPI 383
K NE G E E + + V T YQ + P+ G+T+++ D+NW + +E++ +
Sbjct: 216 KQNENLAFGVEFECNANVGEAVTTLAYQTELPEEGVTMRASFDTNWTVGGVFEKRLSQQL 275
Query: 384 PFSINLCINGDIWKSNYGLGVGFSI 458
PF++ L + K+ G+G I
Sbjct: 276 PFTLALSGTLNHVKAAGKFGIGLII 300
>sp|O13656|TOM40_SCHPO Probable mitochondrial import receptor subunit tom40 (Translocase
of outer membrane 40 kDa subunit)
Length = 344
Score = 42.4 bits (98), Expect = 7e-04
Identities = 35/161 (21%), Positives = 68/161 (42%), Gaps = 12/161 (7%)
Frame = +3
Query: 12 IFDVMRRITDKLSLGSMFVFQRSNQAQFTNFGVWNVGGQYVGNKWTAAVGVRPFQSSLHS 191
I +++ +T KLSLG ++Q+ + + + +Y W A + Q + +
Sbjct: 167 IISLLQSVTPKLSLGVEALWQKPSSSIGPEEATLSYMTRYNAADWIATAHLNGSQGDVTA 226
Query: 192 TFHMKLNEAFQIGTEIETS---LGHRQCVGT---------AGYQFDFPKSGITIKSQVDS 335
TF KL+ + G E + S L H + T G +++F +S + QVDS
Sbjct: 227 TFWRKLSPKVEAGVECQLSPVGLNHSAALMTGPKPEGLTSVGVKYEFAQS--IYRGQVDS 284
Query: 336 NWKIATSYERKFIAPIPFSINLCINGDIWKSNYGLGVGFSI 458
++ ER+ I + + ++ + GLG+ +
Sbjct: 285 KGRVGVYLERRLAPAITLAFSSELDHPNRNAKVGLGLSLEL 325
>sp|P19812|UBR1_YEAST Ubiquitin-protein ligase E3 component N-recognin-1 (N-end-recognizing
protein)
Length = 1950
Score = 31.2 bits (69), Expect = 1.5
Identities = 17/71 (23%), Positives = 35/71 (49%)
Frame = -2
Query: 308 TRFREIKLITSCTHALTMTQTSLNFCTYLKCFIQLHVKS*M*RTLERPNSHCRSPFITHI 129
T+ +EIKL + + L+F L C +++H+++ + N +C PF +
Sbjct: 1676 TQSKEIKLREERSQHMKNADNRLDFKICLTCGVKVHLRADRHEMTKHLNKNCFKPFGAFL 1735
Query: 128 LPTNIPYTEIC 96
+P + +E+C
Sbjct: 1736 MPNS---SEVC 1743
>sp|P27571|XIST_MOUSE X inactive-specific transcript protein
Length = 298
Score = 29.6 bits (65), Expect = 4.4
Identities = 16/58 (27%), Positives = 26/58 (44%), Gaps = 1/58 (1%)
Frame = -2
Query: 386 WYRSYKLSLVRCSDFPITINLRFDCNTRFREIKLITSCTH-ALTMTQTSLNFCTYLKC 216
W L++ C + + I+L F FR+ +T C H +L + T L C+ C
Sbjct: 129 WSALSSLAMALCFIYLVAISLSFSFTPAFRDSLWVTCCMHPSLFLVLTPLVLCSSSLC 186
>sp|P61872|LIP_RHIOR Lipase precursor (Triacylglycerol lipase) (ROL) (RDL)
sp|P61871|LIP_RHINI Lipase precursor (Triacylglycerol lipase) (Lipase II) (RNL)
Length = 392
Score = 28.5 bits (62), Expect = 9.7
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 4/50 (8%)
Frame = +3
Query: 51 LGSMFVFQRSNQAQFTNFGVWNVGGQYVGNK----WTAAVGVRPFQSSLH 188
L M ++QR + N ++ VGG VGN + + G+ PFQ ++H
Sbjct: 276 LAGMDLYQREPRLSPKNLSIFTVGGPRVGNPTFAYYVESTGI-PFQRTVH 324
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,637,957
Number of Sequences: 369166
Number of extensions: 1252581
Number of successful extensions: 2483
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 2432
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2478
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3304927425
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)