Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_J07
(813 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P57795|ISCS_METTE Probable cysteine desulfurase (NifS pr... 125 2e-28
sp|Q43884|NIFS_ANAAZ Cysteine desulfurase (Nitrogenase meta... 122 9e-28
sp|P25374|NFS1_YEAST Cysteine desulfurase, mitochondrial pr... 122 9e-28
sp|P12623|NIFS_ANASP Cysteine desulfurase (Nitrogenase meta... 122 9e-28
sp|P87185|NFS1_CANAL Cysteine desulfurase, mitochondrial pr... 119 1e-26
sp|O60028|NFS1_ASHGO Cysteine desulfurase, mitochondrial pr... 119 1e-26
sp|O25008|ISCS_HELPY Cysteine desulfurase 117 5e-26
sp|Q9ZML2|ISCS_HELPJ Cysteine desulfurase 116 6e-26
sp|O30052|ISC1_ARCFU Probable cysteine desulfurase 1 116 6e-26
sp|Q9ZD60|ISCS_RICPR Cysteine desulfurase (NifS protein hom... 116 8e-26
>sp|P57795|ISCS_METTE Probable cysteine desulfurase (NifS protein homolog)
Length = 404
Score = 125 bits (313), Expect = 2e-28
Identities = 93/248 (37%), Positives = 127/248 (51%), Gaps = 3/248 (1%)
Frame = +3
Query: 78 GAINIEELINCLTEDTALVTVMLANNETGIIQPIRSISMALREYSDNHQFQNRILLHSDA 257
G ++ +EL N + +DT L+++M ANNE G IQPI+ I +E N+I H+DA
Sbjct: 140 GMVSPDELRNAIRDDTILISIMFANNEIGTIQPIKEIGEIAKE--------NQIYFHTDA 191
Query: 258 GQAIGKIDISVDDLGVDYLTIVGHKFCGPR-IGALYVRDLQFNGQSRIYNLKIPIKSSFY 434
QAIG + I V L +D L++ GH+F GP+ GALY+R + I +
Sbjct: 192 VQAIGHVPIDVKKLNIDLLSLSGHEFEGPKGCGALYIRK------------GVKIDPLLH 239
Query: 435 GGGQEGNLRSGTENTPMIVGLGKAAELVTNNINKYFYHMKKIRDYLETCLLIKFSSYGCK 614
GG QE R+GTEN P IVGLGKA EL T I + + K+RD L LL ++
Sbjct: 240 GGAQERKRRAGTENVPGIVGLGKATELATAEIEESNRTLLKLRDRLIEGLLKIPKTH--- 296
Query: 615 ILSRFPDSLDRLPNTCNVALVTNVSITGPE--ILSKCRRVQASVGAACHSIEADRLKSFE 788
L+ P RL N N VT I G +L + + AS G+AC+S S E
Sbjct: 297 -LNGHP--TQRLANNVN---VTFEYIEGESLLLLLNAKGIYASTGSACNS------SSLE 344
Query: 789 GSEILKQC 812
S +L C
Sbjct: 345 PSHVLTAC 352
>sp|Q43884|NIFS_ANAAZ Cysteine desulfurase (Nitrogenase metalloclusters biosynthesis
protein nifS)
Length = 400
Score = 122 bits (307), Expect = 9e-28
Identities = 94/246 (38%), Positives = 124/246 (50%), Gaps = 3/246 (1%)
Frame = +3
Query: 78 GAINIEELINCLTEDTALVTVMLANNETGIIQPIRSISMALREYSDNHQFQNRILLHSDA 257
G ++++EL LT +TALVT+M ANNETG + PI I ++E + H DA
Sbjct: 125 GQLDLDELEASLTGNTALVTIMYANNETGTVFPIEEIGKRVKERG--------AIFHVDA 176
Query: 258 GQAIGKIDISVDDLGVDYLTIVGHKFCGPR-IGALYVRDLQFNGQSRIYNLKIPIKSSFY 434
QA+GKI +++ +D LTI GHK P+ IGALYVR + +
Sbjct: 177 VQAVGKIPLNMKTSTIDMLTISGHKIHAPKGIGALYVRR------------GVRFRPLLI 224
Query: 435 GGGQEGNLRSGTENTPMIVGLGKAAELVTNNINKYFYHMKKIRDYLETCLLIKFSSYGCK 614
GG QE R+GTEN P IVGLGKAAEL +I ++RD LE LL K C+
Sbjct: 225 GGHQERGRRAGTENVPGIVGLGKAAELELIHIETAIKKETRLRDRLEQTLLAKIPD--CE 282
Query: 615 ILSRFPDSLDRLPNTCNVALVTNVSITGPEILSKCRR--VQASVGAACHSIEADRLKSFE 788
+ D RLPNT N+ I G IL + + AS G+AC S S E
Sbjct: 283 VNG---DVTQRLPNTTNIGF---KYIEGEAILLSLNKYGICASSGSACTS------GSLE 330
Query: 789 GSEILK 806
S +L+
Sbjct: 331 PSHVLR 336
>sp|P25374|NFS1_YEAST Cysteine desulfurase, mitochondrial precursor (tRNA splicing
protein SPL1)
Length = 497
Score = 122 bits (307), Expect = 9e-28
Identities = 83/228 (36%), Positives = 119/228 (52%), Gaps = 1/228 (0%)
Frame = +3
Query: 78 GAINIEELINCLTEDTALVTVMLANNETGIIQPIRSISMALREYSDNHQFQNRILLHSDA 257
G I+++EL + + DT LV+VM NNE G+IQPI+ I R+ N+I H+DA
Sbjct: 223 GLIDLKELEDAIRPDTCLVSVMAVNNEIGVIQPIKEIGAICRK--------NKIYFHTDA 274
Query: 258 GQAIGKIDISVDDLGVDYLTIVGHKFCGPR-IGALYVRDLQFNGQSRIYNLKIPIKSSFY 434
QA GKI I V+++ +D L+I HK GP+ IGA+YVR ++ ++
Sbjct: 275 AQAYGKIHIDVNEMNIDLLSISSHKIYGPKGIGAIYVR----------RRPRVRLEPLLS 324
Query: 435 GGGQEGNLRSGTENTPMIVGLGKAAELVTNNINKYFYHMKKIRDYLETCLLIKFSSYGCK 614
GGGQE LRSGT P++ G G+AA L+ + H+K++ D L LL S
Sbjct: 325 GGGQERGLRSGTLAPPLVAGFGEAARLMKKEFDNDQAHIKRLSDKLVKGLL----SAEHT 380
Query: 615 ILSRFPDSLDRLPNTCNVALVTNVSITGPEILSKCRRVQASVGAACHS 758
L+ PD R P NV+ + G +L R + S G+AC S
Sbjct: 381 TLNGSPD--HRYPGCVNVSF---AYVEGESLLMALRDIALSSGSACTS 423
>sp|P12623|NIFS_ANASP Cysteine desulfurase (Nitrogenase metalloclusters biosynthesis
protein nifS)
Length = 400
Score = 122 bits (307), Expect = 9e-28
Identities = 94/246 (38%), Positives = 124/246 (50%), Gaps = 3/246 (1%)
Frame = +3
Query: 78 GAINIEELINCLTEDTALVTVMLANNETGIIQPIRSISMALREYSDNHQFQNRILLHSDA 257
G ++++EL LT +TALVT+M ANNETG + PI I ++E + H DA
Sbjct: 125 GQLDLDELEASLTGNTALVTIMYANNETGTVFPIEEIGKRVKERG--------AIFHVDA 176
Query: 258 GQAIGKIDISVDDLGVDYLTIVGHKFCGPR-IGALYVRDLQFNGQSRIYNLKIPIKSSFY 434
QA+GKI +++ +D LTI GHK P+ IGALYVR + +
Sbjct: 177 VQAVGKIPLNMKTSTIDMLTISGHKIHAPKGIGALYVRR------------GVRFRPLLI 224
Query: 435 GGGQEGNLRSGTENTPMIVGLGKAAELVTNNINKYFYHMKKIRDYLETCLLIKFSSYGCK 614
GG QE R+GTEN P IVGLGKAAEL +I ++RD LE LL K C+
Sbjct: 225 GGHQERGRRAGTENVPGIVGLGKAAELELIHIETAIKKETRLRDRLEQTLLAKIPD--CE 282
Query: 615 ILSRFPDSLDRLPNTCNVALVTNVSITGPEILSKCRR--VQASVGAACHSIEADRLKSFE 788
+ D RLPNT N+ I G IL + + AS G+AC S S E
Sbjct: 283 VNG---DITQRLPNTTNIGF---KYIEGEAILLSLNKYGICASSGSACTS------GSLE 330
Query: 789 GSEILK 806
S +L+
Sbjct: 331 PSHVLR 336
>sp|P87185|NFS1_CANAL Cysteine desulfurase, mitochondrial precursor (tRNA splicing
protein SPL1)
Length = 488
Score = 119 bits (298), Expect = 1e-26
Identities = 79/230 (34%), Positives = 118/230 (51%), Gaps = 3/230 (1%)
Frame = +3
Query: 78 GAINIEELINCLTEDTALVTVMLANNETGIIQPIRSISMALREYSDNHQFQNRILLHSDA 257
G IN+++L + +DT LV++M NNE G+IQP++ I RE N++ H+DA
Sbjct: 212 GLINLDDLKKAIRKDTVLVSIMAVNNEIGVIQPLKEIGKICRE--------NKVFFHTDA 263
Query: 258 GQAIGKIDISVDDLGVDYLTIVGHKFCGPR-IGALYVRDLQFNGQSRIYNLKIPIKSSFY 434
QA GKI I V+++ +D L+I HK GP+ IGA YVR ++ +
Sbjct: 264 AQAYGKIPIDVNEMNIDLLSISSHKIYGPKGIGACYVR----------RRPRVRLDPIIT 313
Query: 435 GGGQEGNLRSGTENTPMIVGLGKAAELVTNNINKYFYHMKKIRDYLETCLLIKFSSY--G 608
GGGQE LRSGT P++ G G+AA L+ + H++K+ L+ LL S+ G
Sbjct: 314 GGGQERGLRSGTLAPPLVAGFGEAARLMKQESSFDKRHIEKLSSKLKNGLLSIPSTQFNG 373
Query: 609 CKILSRFPDSLDRLPNTCNVALVTNVSITGPEILSKCRRVQASVGAACHS 758
C D+ + P NV+ I G +L + + S G+AC S
Sbjct: 374 CN------DAKSQYPGCVNVSF---AYIEGESLLMALKDIALSSGSACTS 414
>sp|O60028|NFS1_ASHGO Cysteine desulfurase, mitochondrial precursor (tRNA splicing
protein SPL1)
Length = 490
Score = 119 bits (298), Expect = 1e-26
Identities = 81/228 (35%), Positives = 117/228 (51%), Gaps = 1/228 (0%)
Frame = +3
Query: 78 GAINIEELINCLTEDTALVTVMLANNETGIIQPIRSISMALREYSDNHQFQNRILLHSDA 257
G +++EEL + +T+LV+VM NNE G++QPI+ I R + ++ HSDA
Sbjct: 216 GLVSLEELEQAIRPETSLVSVMSVNNEIGVVQPIKEIGAICRRH--------KVFFHSDA 267
Query: 258 GQAIGKIDISVDDLGVDYLTIVGHKFCGPR-IGALYVRDLQFNGQSRIYNLKIPIKSSFY 434
QA GKI I VD++ +D L+I HK GP+ IGALYVR ++ ++
Sbjct: 268 AQAYGKIPIDVDEMNIDLLSISSHKIYGPKGIGALYVR----------RRPRVRMEPLLS 317
Query: 435 GGGQEGNLRSGTENTPMIVGLGKAAELVTNNINKYFYHMKKIRDYLETCLLIKFSSYGCK 614
GGGQE RSGT P++VGLG AA+L+ H++++ D L LL S
Sbjct: 318 GGGQERGFRSGTLPPPLVVGLGHAAKLMVEEYEYDSAHVRRLSDRLLKGLL----SIEQT 373
Query: 615 ILSRFPDSLDRLPNTCNVALVTNVSITGPEILSKCRRVQASVGAACHS 758
L+ D R P NV+ + G +L R + S G+AC S
Sbjct: 374 TLNGSAD--HRYPGCVNVSF---AFVEGESLLMALRDIALSSGSACTS 416
>sp|O25008|ISCS_HELPY Cysteine desulfurase
Length = 387
Score = 117 bits (292), Expect = 5e-26
Identities = 84/233 (36%), Positives = 116/233 (49%), Gaps = 6/233 (2%)
Frame = +3
Query: 78 GAINIEELINCLTEDTALVTVMLANNETGIIQPIRSISMALREYSDNHQFQNRILLHSDA 257
G+I E++ +TE TALV+VM ANNETG+I PI I +E +L H+DA
Sbjct: 130 GSITAEQVKEAITEKTALVSVMWANNETGLIFPIEEIGAICKE--------KGVLFHTDA 181
Query: 258 GQAIGKIDISVDDLGVDYLTIVGHKFCGPR-IGALYVRDLQFNGQSRIYNLKIPIKSSFY 434
QAIGKI + V D+L+ HKF GP+ IG LY+R + + F+
Sbjct: 182 VQAIGKIPVDVLKANADFLSFSAHKFHGPKGIGGLYIRS------------GVGLTPLFH 229
Query: 435 GGGQEGNLRSGTENTPMIVGLGKAAELVTNNINKYFYHMKKIRDYLETCLLIKFSSYGCK 614
GG RSGT N P IVG+G+A +L +++ + K+RD LE LL
Sbjct: 230 GGEHMNGRRSGTLNVPYIVGMGEAMKLAVEHLDYEKEVVGKLRDKLEEALL--------- 280
Query: 615 ILSRFPDSL---DRLPNTCNVALVTNVSITGPEILSKCRR--VQASVGAACHS 758
+ PD + DR+ N LV+ I G +L R + AS G+AC S
Sbjct: 281 ---KIPDVMVVGDRIHRVPNTTLVSVRGIEGEAMLWDLNRSNIAASTGSACAS 330
>sp|Q9ZML2|ISCS_HELPJ Cysteine desulfurase
Length = 387
Score = 116 bits (291), Expect = 6e-26
Identities = 83/233 (35%), Positives = 117/233 (50%), Gaps = 6/233 (2%)
Frame = +3
Query: 78 GAINIEELINCLTEDTALVTVMLANNETGIIQPIRSISMALREYSDNHQFQNRILLHSDA 257
G+I +++ +TE TALV+VM ANNETG+I PI I +E +L H+DA
Sbjct: 130 GSITADQVKEAITEKTALVSVMWANNETGLIFPIEEIGAICKE--------KGVLFHTDA 181
Query: 258 GQAIGKIDISVDDLGVDYLTIVGHKFCGPR-IGALYVRDLQFNGQSRIYNLKIPIKSSFY 434
QAIGKI + V VD+L+ HKF GP+ IG LY+R + + F+
Sbjct: 182 VQAIGKIPVDVLKANVDFLSFSAHKFHGPKGIGGLYIRS------------GVGLTPLFH 229
Query: 435 GGGQEGNLRSGTENTPMIVGLGKAAELVTNNINKYFYHMKKIRDYLETCLLIKFSSYGCK 614
GG RSGT N P IVG+G+A +L +++ + K+RD LE LL
Sbjct: 230 GGEHMNGRRSGTLNVPYIVGMGEAMKLAVEHLDYEKEVVGKLRDKLEEALL--------- 280
Query: 615 ILSRFPDSL---DRLPNTCNVALVTNVSITGPEILSKCRR--VQASVGAACHS 758
+ PD + DR+ N L++ I G +L R + AS G+AC S
Sbjct: 281 ---KIPDVMVVGDRVHRVPNTTLISVRGIEGEAMLWDLNRSNIAASTGSACAS 330
>sp|O30052|ISC1_ARCFU Probable cysteine desulfurase 1
Length = 382
Score = 116 bits (291), Expect = 6e-26
Identities = 85/254 (33%), Positives = 128/254 (50%), Gaps = 9/254 (3%)
Frame = +3
Query: 78 GAINIEELINCLTEDTALVTVMLANNETGIIQPIRSISMALREYSDNHQFQNRILLHSDA 257
G +++E + + EDT LV+V ANNE G IQPI+ IS + R LH DA
Sbjct: 124 GTVDLEFIEEKIREDTILVSVQHANNEIGTIQPIKEISEIIN---------GRAALHVDA 174
Query: 258 GQAIGKIDISVDDLGVDYLTIVGHKFCGPR-IGALYVRDLQFNGQSRIYNLKIPIKSSFY 434
++G+I++ V+ +G D LTI + GP+ +GAL+VR + ++
Sbjct: 175 TASLGQIEVDVEKIGADMLTISSNDIYGPKGVGALWVRQ------------GVRLQPIIL 222
Query: 435 GGGQEGNLRSGTENTPMIVGLGKAAELVTNNINKYFYHMKKIRDYLETCLLIKFSSYGCK 614
GGGQE LRSG+EN P IVG GKAAE+ + ++++RD + +L SY
Sbjct: 223 GGGQEKGLRSGSENVPAIVGFGKAAEITAKEWGEEAGRLRRLRDRIIDNVLKIEESY--- 279
Query: 615 ILSRFPDSLDRLPNTCNV--------ALVTNVSITGPEILSKCRRVQASVGAACHSIEAD 770
L+ P+ RLPN NV ++V ++ + G +QAS G+AC S
Sbjct: 280 -LNGHPEK--RLPNNVNVRFSYIEGESIVLSLDMAG---------IQASTGSACSS---- 323
Query: 771 RLKSFEGSEILKQC 812
K+ + S +L C
Sbjct: 324 --KTLQPSHVLMAC 335
>sp|Q9ZD60|ISCS_RICPR Cysteine desulfurase (NifS protein homolog)
Length = 410
Score = 116 bits (290), Expect = 8e-26
Identities = 77/228 (33%), Positives = 111/228 (48%), Gaps = 1/228 (0%)
Frame = +3
Query: 78 GAINIEELINCLTEDTALVTVMLANNETGIIQPIRSISMALREYSDNHQFQNRILLHSDA 257
G I++E L N +T+ T LV+VM NNE G+IQP++ I RE + HSD
Sbjct: 135 GIIDLETLKNAITDQTLLVSVMAVNNEIGVIQPLKEIGKICRE--------RNVFFHSDI 186
Query: 258 GQAIGKIDISVDDLGVDYLTIVGHKFCGPR-IGALYVRDLQFNGQSRIYNLKIPIKSSFY 434
Q GKI I+V++ +D +I GHK GP+ IGALY+R ++ +
Sbjct: 187 AQGFGKIPINVNECNIDLASISGHKIYGPKGIGALYIRK----------KPRVRVTPLIN 236
Query: 435 GGGQEGNLRSGTENTPMIVGLGKAAELVTNNINKYFYHMKKIRDYLETCLLIKFSSYGCK 614
GGGQE +RSGT TP+IVGLG A+E+ N + K H+ + D + K S
Sbjct: 237 GGGQERGMRSGTLPTPLIVGLGIASEIAYNEMEKDTQHVNYLFDRFLNNIHSKISEVYLN 296
Query: 615 ILSRFPDSLDRLPNTCNVALVTNVSITGPEILSKCRRVQASVGAACHS 758
D R N++ + G I+ + + S G+AC S
Sbjct: 297 -----GDKDQRYKGNLNLSF---AGVEGESIILAIKDLAVSSGSACTS 336
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,086,618
Number of Sequences: 369166
Number of extensions: 1786690
Number of successful extensions: 4585
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4373
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4467
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7763237265
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)