Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_I21
(397 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P36168|YK76_YEAST Hypothetical 137.5 kDa protein in MPL1... 33 0.27
sp|P43043|YRR4_MYCCA Hypothetical 31.5 kDa protein in rnpA-... 31 1.0
sp|P27694|RFA1_HUMAN Replication protein A 70 kDa DNA-bindi... 30 2.3
sp|Q89AN8|DPO3A_BUCBP DNA polymerase III alpha subunit 30 2.3
sp|P57655|ILVC_BUCAI Ketol-acid reductoisomerase (Acetohydr... 29 3.0
sp|Q92G24|YD01_RICCN Hypothetical UPF0235 protein RC1301 29 3.9
sp|Q7VMC9|SYM_HAEDU Methionyl-tRNA synthetase (Methionine--... 29 3.9
sp|Q89B37|GYRB_BUCBP DNA gyrase subunit B 29 3.9
sp|P41480|VP33_NPVAC 33 kDa early protein homolog (p33) 28 6.6
sp|P18963|IRA1_YEAST Inhibitory regulator protein IRA1 28 6.6
>sp|P36168|YK76_YEAST Hypothetical 137.5 kDa protein in MPL1-PPC1 intergenic region
Length = 1195
Score = 32.7 bits (73), Expect = 0.27
Identities = 21/86 (24%), Positives = 41/86 (47%)
Frame = +3
Query: 60 ESFVFHQNYLQLIIRNIFQRTKINPENASYLNYLWMILKCYQKDIMKFVKKNRMYLVLIF 239
E+F Y+QL+I NI+QR + N + N L +++++K + L+ F
Sbjct: 477 ETFTPSPQYMQLVIDNIYQRAFVERNNGNLRNSL----------LIEYLKHSEAMLLPSF 526
Query: 240 RPKNNLESYRIRKRIQRTVLDGNTLN 317
+L++ + I++ +D N N
Sbjct: 527 LESPDLQNVVLSYFIEKFGIDANGCN 552
>sp|P43043|YRR4_MYCCA Hypothetical 31.5 kDa protein in rnpA-licA intergenic region (ORF
R4)
Length = 265
Score = 30.8 bits (68), Expect = 1.0
Identities = 21/71 (29%), Positives = 38/71 (53%), Gaps = 6/71 (8%)
Frame = +3
Query: 60 ESFVFHQNYLQLIIRNIFQRTKINPENASYL---NYLWMILKCYQK---DIMKFVKKNRM 221
ESF+FH+N+ + + + F K+N + YL + L + ++C Q+ I F+ KN +
Sbjct: 118 ESFLFHKNWSKDVEKFFFGLEKLN-KTLDYLYDKDLLCLKIECSQETWNKIEDFINKNNL 176
Query: 222 YLVLIFRPKNN 254
+ + F NN
Sbjct: 177 EVNITFNSINN 187
>sp|P27694|RFA1_HUMAN Replication protein A 70 kDa DNA-binding subunit (RP-A) (RF-A)
(Replication factor-A protein 1) (Single-stranded
DNA-binding protein)
Length = 616
Score = 29.6 bits (65), Expect = 2.3
Identities = 28/101 (27%), Positives = 46/101 (45%), Gaps = 2/101 (1%)
Frame = +3
Query: 6 C*SSFFNFKTKIGFKQHLESFVFHQNYLQLIIRNIFQRTK--INPENASYLNYLWMILKC 179
C + F NFK ++ ++ F +Q FQ + I +NA+YL L K
Sbjct: 503 CDTEFPNFKYRMILSVNIADFQENQWV------TCFQESAEAILGQNAAYLGEL----KD 552
Query: 180 YQKDIMKFVKKNRMYLVLIFRPKNNLESYRIRKRIQRTVLD 302
+ + V +N + IFR + +E+Y RI+ TV+D
Sbjct: 553 KNEQAFEEVFQNANFRSFIFRVRVKVETYNDESRIKATVMD 593
>sp|Q89AN8|DPO3A_BUCBP DNA polymerase III alpha subunit
Length = 1077
Score = 29.6 bits (65), Expect = 2.3
Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 7/57 (12%)
Frame = +2
Query: 59 GIFCFPPELSSTNHQ-KYFSENQNQS------RERILFELSMDDFKMLSERYYEICK 208
G CF S H +Y ++ NQ ++R LF L +DDF ++ ++YY I K
Sbjct: 891 GCDCFGLNRSILMHSCEYIIKSANQYLKSIILKKRDLFGLLLDDFNVIKKKYYSIKK 947
>sp|P57655|ILVC_BUCAI Ketol-acid reductoisomerase (Acetohydroxy-acid isomeroreductase)
(Alpha-keto-beta-hydroxylacil reductoisomerase)
Length = 490
Score = 29.3 bits (64), Expect = 3.0
Identities = 17/64 (26%), Positives = 34/64 (53%), Gaps = 2/64 (3%)
Frame = +3
Query: 102 RNIFQRTKINPENASYLNYLWMILKCYQ--KDIMKFVKKNRMYLVLIFRPKNNLESYRIR 275
+ +++ K+ + A Y +YL+ Y K+ + + K+ + L + NN+E YRI
Sbjct: 403 KKLYEMNKVISDTAEYGSYLFSE-SAYPILKEFISTLNKSDLGCALSHQSVNNIELYRIN 461
Query: 276 KRIQ 287
++IQ
Sbjct: 462 QKIQ 465
>sp|Q92G24|YD01_RICCN Hypothetical UPF0235 protein RC1301
Length = 105
Score = 28.9 bits (63), Expect = 3.9
Identities = 21/55 (38%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = +3
Query: 15 SFFNFKTKIGFKQHLES-FVFHQN--YLQLIIRNIFQRTKINPENASYLNYLWMI 170
+ +FK K KQ+L S FV N YL+L I+ I ++ K N E +YL W +
Sbjct: 14 ALLSFKVKPNSKQNLISNFVIINNIPYLKLSIKAIPEQGKANEEIINYLAKEWKL 68
>sp|Q7VMC9|SYM_HAEDU Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS)
Length = 684
Score = 28.9 bits (63), Expect = 3.9
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = +2
Query: 77 PELSSTNHQKYFSENQNQSRERILFELSMDDFKMLSERYYEI--CKEKPD 220
P+++ST ++NQ Q+ E I E+S+DDF L R ++ C+ P+
Sbjct: 557 PQVAST-----MNDNQQQNIEPIAAEISIDDFAKLDLRVAKVIQCEAVPE 601
>sp|Q89B37|GYRB_BUCBP DNA gyrase subunit B
Length = 804
Score = 28.9 bits (63), Expect = 3.9
Identities = 12/43 (27%), Positives = 24/43 (55%)
Frame = +3
Query: 183 QKDIMKFVKKNRMYLVLIFRPKNNLESYRIRKRIQRTVLDGNT 311
Q + K K N+ + + KN +E+Y++ ++ T+L+ NT
Sbjct: 531 QPPLYKVTKNNKTRYI---KDKNTMENYKLSTALENTILESNT 570
>sp|P41480|VP33_NPVAC 33 kDa early protein homolog (p33)
Length = 259
Score = 28.1 bits (61), Expect = 6.6
Identities = 24/104 (23%), Positives = 47/104 (45%), Gaps = 2/104 (1%)
Frame = +3
Query: 45 FKQHLESFVFHQNYLQLIIR--NIFQRTKINPENASYLNYLWMILKCYQKDIMKFVKKNR 218
F ++ +S++ + L ++R TK+ A+YL + ++K KDI K+ +
Sbjct: 8 FSRYKDSYLLYSFRLIDLLRASKSTHLTKLLSSQATYLYHFACLMK--YKDIQKYEVQQL 65
Query: 219 MYLVLIFRPKNNLESYRIRKRIQRTVLDGNTLNPAIHPQRFRTI 350
+ + P +L+ +RI + T L+ + P F TI
Sbjct: 66 IEWAINASPDMDLQQFRIEFMDKTTELNLRSCQPKSFTYTFTTI 109
>sp|P18963|IRA1_YEAST Inhibitory regulator protein IRA1
Length = 3092
Score = 28.1 bits (61), Expect = 6.6
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = +3
Query: 87 LQLIIRNIFQRTKINPENASYLNYLWMI 170
LQ + +N + K NP+++ YL YL+++
Sbjct: 2877 LQALFKNTYYERKSNPKSSDYLCYLFLL 2904
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,126,147
Number of Sequences: 369166
Number of extensions: 769284
Number of successful extensions: 2220
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2219
length of database: 68,354,980
effective HSP length: 97
effective length of database: 50,435,685
effective search space used: 1714813290
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)