Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_I15
(579 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatas... 91 3e-18
sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatas... 88 2e-17
sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromo... 56 7e-08
sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-V... 51 2e-06
sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-... 43 5e-04
sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatas... 38 0.021
sp|Q9HET9|TPP1_SACMI Polynucleotide 3'-phosphatase (2'(3')-... 35 0.13
sp|P54650|HTPG_VIBFI Chaperone protein htpG (Heat shock pro... 32 0.87
sp|Q8Y0Q3|HTPG_RALSO Chaperone protein htpG (Heat shock pro... 32 1.5
sp|P54649|HTPG_ACTAC Chaperone protein htpG (Heat shock pro... 32 1.5
>sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 521
Score = 90.5 bits (223), Expect = 3e-18
Identities = 47/81 (58%), Positives = 63/81 (77%), Gaps = 1/81 (1%)
Frame = +1
Query: 43 AIIEQLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVAD 222
A++E+L V QVLVAT ++ +YRKP TGM+D L ++ N G I+ +S++VGDAAGR A+
Sbjct: 238 AVVEKLGVPFQVLVAT-HAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPAN 296
Query: 223 WAP-KMKKDFSNSDRLFALNL 282
WAP + KKDFS +DRLFALNL
Sbjct: 297 WAPGRKKKDFSCADRLFALNL 317
Score = 47.0 bits (110), Expect = 3e-05
Identities = 30/81 (37%), Positives = 41/81 (50%)
Frame = +2
Query: 287 LKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCPTLMIKYDKFQKSASDIKDILPKDSSEV 466
L F TPE FFL+ F++P F P TV L + + SAS
Sbjct: 319 LPFATPEEFFLKWPAAGFELPAFDP-RTVSRSGPLCLPESRALLSASP------------ 365
Query: 467 VLILMVGYPGSGKSTFCKKYL 529
+++ VG+PG+GKSTF KK+L
Sbjct: 366 EVVVAVGFPGAGKSTFLKKHL 386
>sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 522
Score = 87.8 bits (216), Expect = 2e-17
Identities = 45/81 (55%), Positives = 65/81 (80%), Gaps = 1/81 (1%)
Frame = +1
Query: 43 AIIEQLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVAD 222
A++E+L V QVLVAT ++ + RKP +GM+D L ++ N GI I+ ++S++VGDAAGR+A+
Sbjct: 237 AVLEKLGVPFQVLVAT-HAGLNRKPVSGMWDHLQEQANEGIPISVEDSVFVGDAAGRLAN 295
Query: 223 WAP-KMKKDFSNSDRLFALNL 282
WAP + KKDFS +DRLFALN+
Sbjct: 296 WAPGRKKKDFSCADRLFALNV 316
Score = 42.7 bits (99), Expect = 6e-04
Identities = 30/81 (37%), Positives = 45/81 (55%)
Frame = +2
Query: 287 LKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCPTLMIKYDKFQKSASDIKDILPKDSSEV 466
L F TPE FFL+ F++P F P T+ + L + +S+S L + EV
Sbjct: 318 LPFATPEEFFLKWPAARFELPAFDP-RTISSAGPLYLP-----ESSS-----LLSPNPEV 366
Query: 467 VLILMVGYPGSGKSTFCKKYL 529
V+ VG+PG+GKSTF +++L
Sbjct: 367 VVA--VGFPGAGKSTFIQEHL 385
>sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromosome IV
Length = 407
Score = 55.8 bits (133), Expect = 7e-08
Identities = 34/80 (42%), Positives = 50/80 (62%)
Frame = +1
Query: 43 AIIEQLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVAD 222
AI+ +L + +Q V+ + YRKP GM++ L K N ++IN ES++VGDAAGR+
Sbjct: 124 AIVGKLGIPVQAFVSVAGGH-YRKPCVGMWNEL-KLRNDEVEINEKESIFVGDAAGRIKT 181
Query: 223 WAPKMKKDFSNSDRLFALNL 282
+ KKD S +DR FA N+
Sbjct: 182 -TSRPKKDHSYADRFFAANV 200
Score = 44.3 bits (103), Expect = 2e-04
Identities = 32/82 (39%), Positives = 42/82 (51%), Gaps = 4/82 (4%)
Frame = +2
Query: 287 LKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCPTLMIKYDKFQKSASDIKDILPKD---- 454
+KF TPE FF G+ K+ E P +D + +I ++ P D
Sbjct: 202 VKFQTPEEFF-----GKSKVDE----------PWGPPNFDPKNLFSEEITELEPHDAQLK 246
Query: 455 SSEVVLILMVGYPGSGKSTFCK 520
SSE +ILMVG+PGSGKSTF K
Sbjct: 247 SSEKEIILMVGFPGSGKSTFAK 268
>sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-VUBI intergenic region (ORF 1)
Length = 182
Score = 50.8 bits (120), Expect = 2e-06
Identities = 33/74 (44%), Positives = 47/74 (63%)
Frame = +1
Query: 64 VKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVADWAPKMKK 243
V I V+ N + +RKP+TGM+ + K+ I+ ++S YVGDAAGR+ +K
Sbjct: 99 VPISFYVSPN-KDEHRKPDTGMWREMAKQFTH---IDKEQSFYVGDAAGRIN--LTTGQK 152
Query: 244 DFSNSDRLFALNLS 285
DFS+SDR+FA NLS
Sbjct: 153 DFSDSDRVFAKNLS 166
>sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-polynucleotidase) (DNA 3'
phosphatase) (Three prime phosphatase)
Length = 238
Score = 43.1 bits (100), Expect = 5e-04
Identities = 30/76 (39%), Positives = 37/76 (48%), Gaps = 7/76 (9%)
Frame = +1
Query: 109 RKPNTGMFDLLDKEHNSGIKINCDES-------LYVGDAAGRVADWAPKMKKDFSNSDRL 267
RKP TGM + ++ S +++ S Y GDAAGR KKDFS+SD
Sbjct: 169 RKPMTGMVEFFKRDLESAYRVSEQISPIKLNWIYYCGDAAGR--------KKDFSDSDIK 220
Query: 268 FALNLSSEILYSGSIF 315
FA NL E Y IF
Sbjct: 221 FAENLHVEFKYPEEIF 236
>sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxy-kinase ]
Length = 421
Score = 37.7 bits (86), Expect = 0.021
Identities = 26/60 (43%), Positives = 35/60 (58%)
Frame = +1
Query: 43 AIIEQLAVKIQVLVATNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVAD 222
AI E L + I VL A + +RKP TGM++ K+ N I ++ + YVGDAAGR D
Sbjct: 141 AIFESLDLPI-VLYAAILKDKFRKPLTGMWNSFLKDVNRSIDLSFIK--YVGDAAGRPGD 197
Score = 33.5 bits (75), Expect = 0.39
Identities = 23/82 (28%), Positives = 34/82 (41%), Gaps = 1/82 (1%)
Frame = +2
Query: 287 LKFYTPEAFFLQQEEGEFKMPEFHPAE-TVKNCPTLMIKYDKFQKSASDIKDILPKDSSE 463
+KF TPE FFL FHP V+N + + K S
Sbjct: 211 IKFETPEQFFLGHSFVPPNFESFHPKNYLVRNSSSHPYHFKK---------------SEH 255
Query: 464 VVLILMVGYPGSGKSTFCKKYL 529
++++VG+P SGKST + +
Sbjct: 256 QEIVVLVGFPSSGKSTLAESQI 277
>sp|Q9HET9|TPP1_SACMI Polynucleotide 3'-phosphatase (2'(3')-polynucleotidase) (DNA 3'
phosphatase) (Three prime phosphatase)
Length = 245
Score = 35.0 bits (79), Expect = 0.13
Identities = 27/76 (35%), Positives = 32/76 (42%), Gaps = 7/76 (9%)
Frame = +1
Query: 109 RKPNTGMFDLLDKEHNSGIK-------INCDESLYVGDAAGRVADWAPKMKKDFSNSDRL 267
RKP TGM D + + I + Y GDAAGR K DFS+SD
Sbjct: 176 RKPMTGMADFFRIDITDACRVLKTVPPIKLNWVYYCGDAAGR--------KNDFSDSDIK 227
Query: 268 FALNLSSEILYSGSIF 315
FA L E Y +F
Sbjct: 228 FAEKLHVEFKYPEEVF 243
>sp|P54650|HTPG_VIBFI Chaperone protein htpG (Heat shock protein htpG) (High temperature
protein G)
Length = 391
Score = 32.3 bits (72), Expect = 0.87
Identities = 19/77 (24%), Positives = 38/77 (49%)
Frame = +1
Query: 91 NYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVADWAPKMKKDFSNSDRLF 270
+Y+++ P FD+++++H SG+K+ + DA + + +K ++D
Sbjct: 279 DYTSLLYIPAKAPFDMMNRDHKSGLKLYVQRVFIMDDAEQFMPTYLRFVKGLIDSND--L 336
Query: 271 ALNLSSEILYSGSIFPS 321
LN+S EIL + S
Sbjct: 337 PLNVSREILQDNKVTQS 353
>sp|Q8Y0Q3|HTPG_RALSO Chaperone protein htpG (Heat shock protein htpG) (High temperature
protein G)
Length = 640
Score = 31.6 bits (70), Expect = 1.5
Identities = 20/70 (28%), Positives = 33/70 (47%)
Frame = +1
Query: 88 TNYSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVADWAPKMKKDFSNSDRL 267
+ Y+ + P FDL D+E SG+K+ + DA + + +K ++D
Sbjct: 277 SEYTQLLYLPTNAPFDLWDRERRSGLKLYVKRVFIMDDAEQLLPAYLRFVKGVIDSAD-- 334
Query: 268 FALNLSSEIL 297
LN+S EIL
Sbjct: 335 LPLNVSREIL 344
>sp|P54649|HTPG_ACTAC Chaperone protein htpG (Heat shock protein htpG) (High temperature
protein G)
Length = 626
Score = 31.6 bits (70), Expect = 1.5
Identities = 18/73 (24%), Positives = 36/73 (49%)
Frame = +1
Query: 94 YSNVYRKPNTGMFDLLDKEHNSGIKINCDESLYVGDAAGRVADWAPKMKKDFSNSDRLFA 273
Y+++ P+ +DL ++EH G+K+ + DA + ++ M+ ++D
Sbjct: 277 YTSLLYVPSKAPWDLFNREHKHGLKLYVQRVFIMDDAEQFMPNYLRFMRGLIDSND--LP 334
Query: 274 LNLSSEILYSGSI 312
LN+S EIL +
Sbjct: 335 LNVSREILQDNKV 347
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,282,449
Number of Sequences: 369166
Number of extensions: 1231037
Number of successful extensions: 4598
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4472
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4587
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4259329035
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)