Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_I13
(152 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P52434|RPB8_HUMAN DNA-directed RNA polymerases I, II, an... 36 0.032
sp|Q19826|RPB8_CAEEL Probable DNA-directed RNA polymerases ... 33 0.21
>sp|P52434|RPB8_HUMAN DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide
(RPB17) (RPB8) (RPABC3)
sp|Q923G2|RPB8_MOUSE DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide
(RPB17) (RPB8)
Length = 150
Score = 35.8 bits (81), Expect = 0.032
Identities = 16/25 (64%), Positives = 19/25 (76%)
Frame = +3
Query: 57 KGAATNLSGFKVGQHIYLLMKKLAF 131
+G A NL GF+V +YLLMKKLAF
Sbjct: 126 QGDANNLHGFEVDSRVYLLMKKLAF 150
>sp|Q19826|RPB8_CAEEL Probable DNA-directed RNA polymerases I, II, and III 17.1 kDa
polypeptide (RPB17) (RPB8)
Length = 148
Score = 33.1 bits (74), Expect = 0.21
Identities = 15/25 (60%), Positives = 18/25 (72%)
Frame = +3
Query: 57 KGAATNLSGFKVGQHIYLLMKKLAF 131
KG A NL GF+V ++YLLMKK F
Sbjct: 124 KGEAINLHGFEVDMNLYLLMKKTDF 148
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,546,402
Number of Sequences: 369166
Number of extensions: 180128
Number of successful extensions: 254
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 254
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 254
length of database: 68,354,980
effective HSP length: 23
effective length of database: 64,106,075
effective search space used: 1730864025
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)