Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_I10
(359 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P23785|GRN_RAT Granulins precursor [Contains: Acrogranin... 39 0.004
sp|P28799|GRN_HUMAN Granulins precursor (Proepithelin) (PEP... 37 0.011
sp|P28798|GRN_MOUSE Granulins precursor (Acrogranin) (Proep... 37 0.018
sp|P28797|GRN_CAVPO Granulins precursor (Proepithelin) (PEP... 36 0.031
sp|P80930|ENA1_HORSE Antimicrobial peptide eNAP-1 35 0.040
sp|P34504|YMV2_CAEEL Hypothetical protein K04H4.2 precursor 33 0.20
sp|P15265|MCSP_MOUSE Sperm mitochondrial associated cystein... 30 1.3
sp|P07987|GUX2_TRIRE Exoglucanase II precursor (Exocellobio... 30 1.3
sp|Q13615|MTMR3_HUMAN Myotubularin-related protein 3 (FYVE ... 30 1.7
sp|Q8FDN6|MASZ_ECOL6 Malate synthase G 29 2.9
>sp|P23785|GRN_RAT Granulins precursor [Contains: Acrogranin; Granulin-1 (Granulin G);
Granulin-2 (Granulin F); Granulin-3 (Granulin B)
(Epithelin-2); Granulin-4 (Granulin A) (Epithelin-1);
Granulin-5 (Granulin C); Granulin-6 (Granulin D);
Granulin-7 (Granulin E)]
Length = 588
Score = 38.9 bits (89), Expect = 0.004
Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 4/45 (8%)
Frame = +2
Query: 119 SFKSSLWNRWCPGNYYCHSSQTCCAPGR----CCPYPYVNCCPGG 241
+F S + N C ++CH +Q+CC + CCPY CC G
Sbjct: 506 TFGSKVGNVECGAGHFCHDNQSCCKDSQGGWACCPYVKGVCCRDG 550
Score = 33.5 bits (75), Expect = 0.15
Identities = 14/33 (42%), Positives = 17/33 (51%), Gaps = 5/33 (15%)
Frame = +2
Query: 149 CPGNYY-CHSSQTCC----APGRCCPYPYVNCC 232
CPG+ + C S TCC CCP P +CC
Sbjct: 125 CPGSQFECPDSATCCIMIDGSWGCCPMPQASCC 157
Score = 30.0 bits (66), Expect = 1.7
Identities = 19/58 (32%), Positives = 24/58 (41%), Gaps = 4/58 (6%)
Frame = +2
Query: 71 PQVVKVKASKSIVTKSSFKSSLWNRWCPGNYYCHSSQTCCAPGR----CCPYPYVNCC 232
P + KV AS S+ K+ + C C S+ TCC CCP P CC
Sbjct: 340 PWMKKVTASLSLPDPQILKNDVP---CDDFSSCPSNNTCCRLSSGDWGCCPMPEAVCC 394
>sp|P28799|GRN_HUMAN Granulins precursor (Proepithelin) (PEPI) [Contains: Acrogranin;
Paragranulin; Granulin-1 (Granulin G); Granulin-2
(Granulin F); Granulin-3 (Granulin B); Granulin-4
(Granulin A); Granulin-5 (Granulin C); Granulin-6
(Granulin D); Granulin-7 (Granulin E)]
Length = 593
Score = 37.4 bits (85), Expect = 0.011
Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 4/32 (12%)
Frame = +2
Query: 149 CPGNYYCHSSQTCCAPGR----CCPYPYVNCC 232
C ++CH +QTCC R CCPY CC
Sbjct: 521 CGEGHFCHDNQTCCRDNRQGWACCPYRQGVCC 552
Score = 29.6 bits (65), Expect = 2.2
Identities = 16/45 (35%), Positives = 19/45 (42%), Gaps = 12/45 (26%)
Frame = +2
Query: 149 CPGNYY-CHSSQTCCA----PGRCCPYPY-------VNCCPGGLF 247
CP + + C TCC CCP P V+CCP G F
Sbjct: 126 CPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAF 170
Score = 28.9 bits (63), Expect = 3.8
Identities = 13/32 (40%), Positives = 13/32 (40%), Gaps = 4/32 (12%)
Frame = +2
Query: 149 CPGNYYCHSSQTCC----APGRCCPYPYVNCC 232
C C SS TCC CCP P CC
Sbjct: 366 CDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCC 397
>sp|P28798|GRN_MOUSE Granulins precursor (Acrogranin) (Proepithelin) (PEPI) (PC
cell-derived growth factor) (PCDGF) [Contains: Granulin
1; Granulin 2; Granulin 3; Granulin 4; Granulin 5;
Granulin 6; Granulin 7]
Length = 589
Score = 36.6 bits (83), Expect = 0.018
Identities = 16/42 (38%), Positives = 20/42 (47%), Gaps = 11/42 (26%)
Frame = +2
Query: 149 CPGNYYCHSSQTCCAPG----RCCPY-------PYVNCCPGG 241
C ++CH +QTCC CCPY +CCPGG
Sbjct: 517 CGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGG 558
Score = 33.5 bits (75), Expect = 0.15
Identities = 14/33 (42%), Positives = 17/33 (51%), Gaps = 5/33 (15%)
Frame = +2
Query: 149 CPGNYY-CHSSQTCC----APGRCCPYPYVNCC 232
CPG+ + C S TCC CCP P +CC
Sbjct: 125 CPGSQFECPDSATCCIMVDGSWGCCPMPQASCC 157
>sp|P28797|GRN_CAVPO Granulins precursor (Proepithelin) (PEPI) [Contains: Acrogranin;
Granulin-1; Granulin-2; Granulin-3; Granulin-4;
Granulin-5; Granulin-6; Granulin-7]
Length = 591
Score = 35.8 bits (81), Expect = 0.031
Identities = 13/32 (40%), Positives = 16/32 (50%), Gaps = 4/32 (12%)
Frame = +2
Query: 149 CPGNYYCHSSQTCCAPGR----CCPYPYVNCC 232
C ++CH QTCC R CCP+ CC
Sbjct: 518 CGDRHFCHDEQTCCRDSRGGWACCPFHQGVCC 549
Score = 34.3 bits (77), Expect = 0.090
Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 11/58 (18%)
Frame = +2
Query: 95 SKSIVTKSSFKSSLWNRWCPGNYYCHSSQTCCA--PGR--CCPYP-------YVNCCP 235
+K+++TK ++W+ C C QTCC G+ CCP+P +V+CCP
Sbjct: 266 AKTLLTKLP-SWTVWDVECDQEVSCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCP 322
Score = 33.1 bits (74), Expect = 0.20
Identities = 14/33 (42%), Positives = 16/33 (48%), Gaps = 5/33 (15%)
Frame = +2
Query: 149 CPGNYY-CHSSQTCC----APGRCCPYPYVNCC 232
CPG + C S TCC CCP P +CC
Sbjct: 112 CPGGEFECPDSSTCCHMLDGSWGCCPMPQASCC 144
>sp|P80930|ENA1_HORSE Antimicrobial peptide eNAP-1
Length = 46
Score = 35.4 bits (80), Expect = 0.040
Identities = 13/32 (40%), Positives = 16/32 (50%), Gaps = 4/32 (12%)
Frame = +2
Query: 149 CPGNYYCHSSQTCCAPGR----CCPYPYVNCC 232
C ++CH QTCC + CCPY CC
Sbjct: 4 CGEGHFCHDXQTCCRASQGGXACCPYSQGVCC 35
>sp|P34504|YMV2_CAEEL Hypothetical protein K04H4.2 precursor
Length = 967
Score = 33.1 bits (74), Expect = 0.20
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = +2
Query: 158 NYYCHSSQTCCAPGRCCPYPYVNCCPGG 241
+YYC S TC CCP +N CP G
Sbjct: 550 SYYCGSGYTCTTGNICCP---INSCPNG 574
>sp|P15265|MCSP_MOUSE Sperm mitochondrial associated cysteine-rich protein
Length = 143
Score = 30.4 bits (67), Expect = 1.3
Identities = 12/29 (41%), Positives = 12/29 (41%)
Frame = +2
Query: 149 CPGNYYCHSSQTCCAPGRCCPYPYVNCCP 235
CP C CC CCP P CCP
Sbjct: 21 CPPKPCCPQKPPCCPKSPCCP-PKSPCCP 48
>sp|P07987|GUX2_TRIRE Exoglucanase II precursor (Exocellobiohydrolase II) (CBHII)
(1,4-beta-cellobiohydrolase)
Length = 471
Score = 30.4 bits (67), Expect = 1.3
Identities = 17/40 (42%), Positives = 20/40 (50%), Gaps = 3/40 (7%)
Frame = +2
Query: 128 SSLWNRWCPGNYYCHSSQTCCAPGRCCPYP---YVNCCPG 238
SS+W + C G + S TCCA G C Y Y C PG
Sbjct: 28 SSVWGQ-CGGQNW--SGPTCCASGSTCVYSNDYYSQCLPG 64
>sp|Q13615|MTMR3_HUMAN Myotubularin-related protein 3 (FYVE domain-containing dual
specificity protein phosphatase 1) (FYVE-DSP1) (Zinc
finger FYVE domain containing protein 10)
Length = 1198
Score = 30.0 bits (66), Expect = 1.7
Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = -1
Query: 320 IKRSLSIIICNSVHIHNNNIHFDSKIIHQDSNLRKGMDNICQVHNKFVKSDN-SNFLDTI 144
+ R LS + C+S H+H+ N+H K +H S + Q + D S + DTI
Sbjct: 970 LSRQLSAMSCSSAHLHSRNLH--HKWLHSHSGRPSATSSPDQPSRSHLDDDGMSVYTDTI 1027
>sp|Q8FDN6|MASZ_ECOL6 Malate synthase G
Length = 723
Score = 29.3 bits (64), Expect = 2.9
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = -1
Query: 278 IHNNNIHFDSKIIHQDSNLRKGMDNICQVHNKFVKSDNSNFLDTIDSITMI 126
+ NN +H + +I D+N R G D+ +++ V++ S LD DS+ +
Sbjct: 231 LKNNGLHIELQI---DANGRIGKDDSAHINDVIVEAAISTILDCEDSVAAV 278
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,933,557
Number of Sequences: 369166
Number of extensions: 531981
Number of successful extensions: 2080
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1908
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2058
length of database: 68,354,980
effective HSP length: 87
effective length of database: 52,283,035
effective search space used: 1673057120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)