Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_H24
(431 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (I... 52 4e-07
sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (I... 50 2e-06
sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A) 50 2e-06
sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B) 50 3e-06
sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (I... 49 6e-06
sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (I... 49 6e-06
sp|P31732|OV71_ONCVO Muscle cell intermediate filament prot... 48 1e-05
sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (I... 47 2e-05
sp|P21910|LAML2_XENLA Lamin L(II) 46 3e-05
sp|P14732|LAM2_CHICK Lamin B2 46 4e-05
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
A4) (IF-A4) (Cel IF A4)
Length = 575
Score = 52.4 bits (124), Expect = 4e-07
Identities = 28/83 (33%), Positives = 49/83 (59%)
Frame = +2
Query: 122 ETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILENIGTKVESLEGMTIKRAIEGNSNQI 301
ETT + S + R+ KGNI+I E + +G +I+LENI + E++ ++R I G +I
Sbjct: 453 ETTSHSS----YSRSAKGNIAIQEAEPSGKYIVLENISRRDENIGDWKLRRKIAG-KREI 507
Query: 302 SYTISSKVTLNAGEKISLVSRGK 370
+T + TL A + + + +RG+
Sbjct: 508 VFTFpreFTLRAQKSVKIFARGQ 530
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
A2) (IF-A2) (Cel IF A2)
Length = 581
Score = 50.1 bits (118), Expect = 2e-06
Identities = 30/87 (34%), Positives = 46/87 (52%), Gaps = 1/87 (1%)
Frame = +2
Query: 113 TERETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGN 289
T R S R ++R+ KGN++I ET G F+ILEN K E L +KR I+G
Sbjct: 447 TMRVVKGEHSSRTSYQRSAKGNVAIKETSPEGKFVILENTHRAKEEPLGDWKLKRKIDG- 505
Query: 290 SNQISYTISSKVTLNAGEKISLVSRGK 370
+I +T S L+ + + + +RG+
Sbjct: 506 KREIVFTFPSDYILHPFQSVKIFARGQ 532
>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
Length = 497
Score = 50.1 bits (118), Expect = 2e-06
Identities = 27/78 (34%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
Frame = +2
Query: 140 SKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGNSNQISYTIS 316
+ R F+R+ KGN+SI ET G +++LEN +K E++ +KR I+G +I YT+
Sbjct: 374 ASRTSFQRSAKGNVSIQETAPDGRYVVLENTHRSKEEAIGEWKLKRKIDG-KREIVYTLP 432
Query: 317 SKVTLNAGEKISLVSRGK 370
L G+ + + +RG+
Sbjct: 433 RDFILRPGKSVKIWARGQ 450
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
Length = 589
Score = 49.7 bits (117), Expect = 3e-06
Identities = 29/76 (38%), Positives = 44/76 (57%), Gaps = 1/76 (1%)
Frame = +2
Query: 140 SKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGNSNQISYTIS 316
S + F+R+ KGN++I+E D G FI LEN +K E+L +KR ++ N +I YTI
Sbjct: 465 STKTTFQRSAKGNVTISECDPNGKFITLENTHRSKDENLGEHRLKRKLD-NRREIVYTIP 523
Query: 317 SKVTLNAGEKISLVSR 364
L AG + + +R
Sbjct: 524 PNTVLKAGRTMKIYAR 539
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
A3) (IF-A3) (Cel IF A3)
Length = 581
Score = 48.5 bits (114), Expect = 6e-06
Identities = 31/95 (32%), Positives = 46/95 (48%), Gaps = 1/95 (1%)
Frame = +2
Query: 113 TERETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGN 289
T R S R ++R+ KGN+SI E G F+ILEN K E L +KR I+G
Sbjct: 447 TMRVVKGEHSSRTSYQRSAKGNVSIKEVSPEGKFVILENTHRDKEEPLGDWKLKRKIDG- 505
Query: 290 SNQISYTISSKVTLNAGEKISLVSRGKKTGKGLEI 394
+I +T S L+ + + + +RG E+
Sbjct: 506 KREIVFTFPSDYILHPVQTVKIFARGNGVANPPEV 540
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
A1) (IF-A1) (Cel IF A1)
Length = 575
Score = 48.5 bits (114), Expect = 6e-06
Identities = 28/87 (32%), Positives = 45/87 (51%), Gaps = 1/87 (1%)
Frame = +2
Query: 113 TERETTDYQSKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGN 289
T R + R F+R+ KGN+SI E G FI+L+N K E++ +KR I+G
Sbjct: 445 TMRVVKGETASRQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGK 504
Query: 290 SNQISYTISSKVTLNAGEKISLVSRGK 370
+ YT+ L AG+ + + +R +
Sbjct: 505 RENV-YTLPRDFVLRAGKTLKIFARNQ 530
>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
Length = 432
Score = 47.8 bits (112), Expect = 1e-05
Identities = 27/78 (34%), Positives = 43/78 (55%), Gaps = 1/78 (1%)
Frame = +2
Query: 140 SKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGNSNQISYTIS 316
+ R F+R+ KGN+SI + G FI+LEN +K E + +KR I+G +I YT
Sbjct: 309 ASRTSFQRSAKGNVSIQDASSDGKFILLENTHRSKEEPIGEWRLKRKIDG-KREIVYTFP 367
Query: 317 SKVTLNAGEKISLVSRGK 370
L G+ + + +RG+
Sbjct: 368 RDFILKPGKTVKIWARGQ 385
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
B1) (IF-B1) (Cel IF B1)
Length = 589
Score = 46.6 bits (109), Expect = 2e-05
Identities = 26/76 (34%), Positives = 46/76 (60%), Gaps = 1/76 (1%)
Frame = +2
Query: 140 SKRMKFRRTTKGNISIAETDLTGSFIILENI-GTKVESLEGMTIKRAIEGNSNQISYTIS 316
S + F+R+ KGN++I+E D G FI LEN K E++ I+R ++G +I Y+I
Sbjct: 465 STKTTFQRSAKGNVTISECDPNGKFIKLENSHRNKDENVGEHKIRRKLDGR-REIVYSIP 523
Query: 317 SKVTLNAGEKISLVSR 364
+ V + G+ +++ +R
Sbjct: 524 ANVVIKPGKNLTIYAR 539
>sp|P21910|LAML2_XENLA Lamin L(II)
Length = 623
Score = 46.2 bits (108), Expect = 3e-05
Identities = 29/87 (33%), Positives = 44/87 (50%), Gaps = 4/87 (4%)
Frame = +2
Query: 113 TERETTDYQSKRMKF----RRTTKGNISIAETDLTGSFIILENIGTKVESLEGMTIKRAI 280
+ R T QS +F + + G+ISI E DL G ++ L+N K +SL +KR I
Sbjct: 454 SSRTITSGQSSTTRFHLSQQASATGSISIEEIDLEGKYVHLKNNSDKDQSLGNWRLKRKI 513
Query: 281 EGNSNQISYTISSKVTLNAGEKISLVS 361
G +I Y + K L AG+ + + S
Sbjct: 514 -GEEEEIVYKFTPKYVLKAGQSVKIYS 539
>sp|P14732|LAM2_CHICK Lamin B2
Length = 600
Score = 45.8 bits (107), Expect = 4e-05
Identities = 25/72 (34%), Positives = 42/72 (58%)
Frame = +2
Query: 140 SKRMKFRRTTKGNISIAETDLTGSFIILENIGTKVESLEGMTIKRAIEGNSNQISYTISS 319
S +M + + G+ISI E DL G ++ L+N K +SL +KR I G+ +I+Y +
Sbjct: 444 SFQMSQQASATGSISIEEIDLEGKYVQLKNNSEKDQSLGNWRLKRQI-GDGEEIAYKFTP 502
Query: 320 KVTLNAGEKISL 355
K L AG+ +++
Sbjct: 503 KYVLRAGQTVTI 514
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,725,321
Number of Sequences: 369166
Number of extensions: 525413
Number of successful extensions: 1413
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1398
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1411
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2144903640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)