Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_H05
(910 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8U3Y2|GYAR_PYRFU Glyoxylate reductase (Glycolate reduct... 67 9e-11
sp|Q5JEZ2|GYAR_PYRKO Glyoxylate reductase (Glycolate reduct... 62 3e-09
sp|O58320|GYAR_PYRHO Glyoxylate reductase (Glycolate reduct... 61 4e-09
sp|Q9UYR1|GYAR_PYRAB Glyoxylate reductase (Glycolate reduct... 59 2e-08
sp|P58000|TKRA_ENTAG 2-ketogluconate reductase (2KR) (2-ket... 56 2e-07
sp|Q9C4M5|GYAR_THELI Glyoxylate reductase (Glycolate reduct... 55 2e-07
sp|Q9YAW4|GYAR_AERPE Glyoxylate reductase (Glycolate reduct... 55 2e-07
sp|O32264|TKRA_BACSU Probable 2-ketogluconate reductase (2KR) 52 2e-06
sp|Q9UBQ7|GRHPR_HUMAN Glyoxylate reductase/hydroxypyruvate ... 46 1e-04
sp|P45250|YF56_HAEIN Putative 2-hydroxyacid dehydrogenase H... 44 6e-04
>sp|Q8U3Y2|GYAR_PYRFU Glyoxylate reductase (Glycolate reductase)
Length = 336
Score = 66.6 bits (161), Expect = 9e-11
Identities = 34/77 (44%), Positives = 51/77 (66%), Gaps = 3/77 (3%)
Frame = +1
Query: 688 LGQCLAHKASVGLGMKIMYHQRNKKSNS---LFPYYETLNEMLPEVDFLVISCPLTNQTK 858
+GQ +A +A G M+I+Y+ R +KS + L Y L E+L E DF++++ PLT +T
Sbjct: 161 IGQAIARRAK-GFNMRILYYSRTRKSQAEKELGAEYRPLEEVLKESDFVILAVPLTKETM 219
Query: 859 HFINLDRMKLMKPTAFL 909
+ IN +R+KLMKPTA L
Sbjct: 220 YMINEERLKLMKPTAIL 236
>sp|Q5JEZ2|GYAR_PYRKO Glyoxylate reductase (Glycolate reductase)
Length = 333
Score = 61.6 bits (148), Expect = 3e-09
Identities = 31/77 (40%), Positives = 53/77 (68%), Gaps = 3/77 (3%)
Frame = +1
Query: 688 LGQCLAHKASVGLGMKIMYHQRNKKSNS---LFPYYETLNEMLPEVDFLVISCPLTNQTK 858
+GQ +A +A G GM+I+Y+ R++K + L + +L ++L E DF+V++ PLT +T+
Sbjct: 161 IGQAVARRAR-GFGMRILYYSRSRKPEAEKELGAEFRSLEDLLRESDFVVLAVPLTKETQ 219
Query: 859 HFINLDRMKLMKPTAFL 909
+ IN +R++LMK TA L
Sbjct: 220 YMINEERLRLMKKTAIL 236
Score = 37.7 bits (86), Expect = 0.045
Identities = 15/54 (27%), Positives = 33/54 (61%)
Frame = +2
Query: 257 PNIRVISCMSSGTDYLDMQKLYSKNILIKNCPDETADSTSEVALLLILNLSRKI 418
P +R+++ + G D +D+++ + I + N PD D+T++ A L+L +R++
Sbjct: 66 PRLRIVANYAVGYDNIDVEEATRRGIYVTNTPDVLTDATADFAWTLLLATARRL 119
>sp|O58320|GYAR_PYRHO Glyoxylate reductase (Glycolate reductase)
Length = 334
Score = 61.2 bits (147), Expect = 4e-09
Identities = 32/77 (41%), Positives = 49/77 (63%), Gaps = 3/77 (3%)
Frame = +1
Query: 688 LGQCLAHKASVGLGMKIMYHQRNKKSN---SLFPYYETLNEMLPEVDFLVISCPLTNQTK 858
+GQ +A +A G M+I+Y+ R +K L ++ L ++L E DF+V++ PLT +T
Sbjct: 161 IGQAIAKRAK-GFNMRILYYSRTRKEEVERELNAEFKPLEDLLRESDFVVLAVPLTRETY 219
Query: 859 HFINLDRMKLMKPTAFL 909
H IN +R+KLMK TA L
Sbjct: 220 HLINEERLKLMKKTAIL 236
Score = 42.4 bits (98), Expect = 0.002
Identities = 17/57 (29%), Positives = 35/57 (61%)
Frame = +2
Query: 257 PNIRVISCMSSGTDYLDMQKLYSKNILIKNCPDETADSTSEVALLLILNLSRKIPKG 427
P +R+++ + G D +D+++ + I + N PD D+T+++A L+L +R + KG
Sbjct: 66 PKLRIVANYAVGYDNIDIEEATKRGIYVTNTPDVLTDATADLAFALLLATARHVVKG 122
>sp|Q9UYR1|GYAR_PYRAB Glyoxylate reductase (Glycolate reductase)
Length = 335
Score = 58.9 bits (141), Expect = 2e-08
Identities = 31/77 (40%), Positives = 49/77 (63%), Gaps = 3/77 (3%)
Frame = +1
Query: 688 LGQCLAHKASVGLGMKIMYHQRNKK---SNSLFPYYETLNEMLPEVDFLVISCPLTNQTK 858
+GQ +A +A G M+I+Y+ R +K L ++ L+E+L E DF+V++ PL +T
Sbjct: 162 IGQAIAKRAR-GFDMRILYYSRTRKPEVEKELNAEFKPLDELLRESDFVVLAVPLNKETY 220
Query: 859 HFINLDRMKLMKPTAFL 909
H IN +R+K+MK TA L
Sbjct: 221 HMINEERLKMMKRTAIL 237
Score = 39.7 bits (91), Expect = 0.012
Identities = 17/59 (28%), Positives = 35/59 (59%)
Frame = +2
Query: 257 PNIRVISCMSSGTDYLDMQKLYSKNILIKNCPDETADSTSEVALLLILNLSRKIPKGLK 433
P +R+++ + G D +D+++ + I + N P D+T+++A L+L +R + KG K
Sbjct: 67 PRLRIVANYAVGYDNIDVEEATKRGIYVTNTPGVLTDATADLAFALLLATARHLVKGDK 125
>sp|P58000|TKRA_ENTAG 2-ketogluconate reductase (2KR) (2-ketoaldonate reductase)
Length = 323
Score = 55.8 bits (133), Expect = 2e-07
Identities = 33/77 (42%), Positives = 47/77 (61%), Gaps = 3/77 (3%)
Frame = +1
Query: 688 LGQCLAHKASVGLGMKIMYHQR--NKKSNSLF-PYYETLNEMLPEVDFLVISCPLTNQTK 858
+G LA +A G GM I+Y+ R ++++ S F Y L+ +L E DFL IS PLT QT
Sbjct: 155 IGLALAQRAHHGFGMPILYNARKHHEEAESRFNAQYCDLDTLLRESDFLCISLPLTEQTH 214
Query: 859 HFINLDRMKLMKPTAFL 909
H I +++ MKP+A L
Sbjct: 215 HMIGREQLAKMKPSAIL 231
>sp|Q9C4M5|GYAR_THELI Glyoxylate reductase (Glycolate reductase)
Length = 331
Score = 55.5 bits (132), Expect = 2e-07
Identities = 31/77 (40%), Positives = 43/77 (55%), Gaps = 3/77 (3%)
Frame = +1
Query: 688 LGQCLAHKASVGLGMKIMYHQRNKKSNS---LFPYYETLNEMLPEVDFLVISCPLTNQTK 858
+GQ LA +A G GMKI+Y+ R +K + + Y +L E DF+ + PLT +T
Sbjct: 161 IGQALAKRAK-GFGMKIIYYSRTRKPEAEEEIGAEYVDFETLLKESDFISLHVPLTKETY 219
Query: 859 HFINLDRMKLMKPTAFL 909
H I +KLMKP A L
Sbjct: 220 HMIGEKELKLMKPNAIL 236
Score = 37.7 bits (86), Expect = 0.045
Identities = 15/54 (27%), Positives = 34/54 (62%)
Frame = +2
Query: 257 PNIRVISCMSSGTDYLDMQKLYSKNILIKNCPDETADSTSEVALLLILNLSRKI 418
P +++I+ + G D +D+++ + I + N P D+T+++A L+L ++R+I
Sbjct: 66 PKLKIIAQYAVGYDNIDIEEATKRGIYVTNTPGVLTDATADLAFALLLAVARRI 119
>sp|Q9YAW4|GYAR_AERPE Glyoxylate reductase (Glycolate reductase)
Length = 335
Score = 55.5 bits (132), Expect = 2e-07
Identities = 31/79 (39%), Positives = 46/79 (58%), Gaps = 5/79 (6%)
Frame = +1
Query: 688 LGQCLAHKASVG--LGMKIMYHQRNKK---SNSLFPYYETLNEMLPEVDFLVISCPLTNQ 852
+G+ + A +G GM+I+YH R++K L Y +L ++L E D L I PLT++
Sbjct: 159 MGRIGSRVAEIGKAFGMRIIYHSRSRKREIEKELGAEYRSLEDLLRESDILSIHLPLTDE 218
Query: 853 TKHFINLDRMKLMKPTAFL 909
T+H I +KLMK TA L
Sbjct: 219 TRHLIGESELKLMKKTAIL 237
Score = 33.5 bits (75), Expect = 0.84
Identities = 15/54 (27%), Positives = 30/54 (55%)
Frame = +2
Query: 257 PNIRVISCMSSGTDYLDMQKLYSKNILIKNCPDETADSTSEVALLLILNLSRKI 418
P +R+++ M+ G D +D++ I + N P ++T+E LIL +R++
Sbjct: 67 PRLRIVAQMAVGFDNIDVECATRLGIYVTNTPGVLTEATAEFTWALILAAARRV 120
>sp|O32264|TKRA_BACSU Probable 2-ketogluconate reductase (2KR)
Length = 325
Score = 52.4 bits (124), Expect = 2e-06
Identities = 26/75 (34%), Positives = 45/75 (60%), Gaps = 3/75 (4%)
Frame = +1
Query: 688 LGQCLAHKASVGLGMKIMYHQRNKKS---NSLFPYYETLNEMLPEVDFLVISCPLTNQTK 858
+G+ A +A G M+++YH R++K +S+ Y L+ +L + DF+++ PLT++T
Sbjct: 159 IGEQAARRAKFGFDMEVLYHNRHRKQETEDSIGVKYAELDTLLEQSDFILLITPLTDETY 218
Query: 859 HFINLDRMKLMKPTA 903
H I KLMK +A
Sbjct: 219 HMIGEREFKLMKNSA 233
>sp|Q9UBQ7|GRHPR_HUMAN Glyoxylate reductase/hydroxypyruvate reductase
Length = 328
Score = 46.2 bits (108), Expect = 1e-04
Identities = 20/58 (34%), Positives = 38/58 (65%)
Frame = +2
Query: 260 NIRVISCMSSGTDYLDMQKLYSKNILIKNCPDETADSTSEVALLLILNLSRKIPKGLK 433
N++VIS MS G D+L + ++ + I + PD D+T+E+A+ L+L R++P+ ++
Sbjct: 74 NLKVISTMSVGIDHLALDEIKKRGIRVGYTPDVLTDTTAELAVSLLLTTCRRLPEAIE 131
Score = 32.3 bits (72), Expect = 1.9
Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 3/75 (4%)
Frame = +1
Query: 688 LGQCLAHKASVGLGMKIMYHQRNKKSNSLFPY---YETLNEMLPEVDFLVISCPLTNQTK 858
+GQ +A + + +Y R + + + + E+ + DF+V++C LT T+
Sbjct: 164 IGQAIARRLKPFGVQRFLYTGRQPRPEEAAEFQAEFVSTPELAAQSDFIVVACSLTPATE 223
Query: 859 HFINLDRMKLMKPTA 903
N D + MK TA
Sbjct: 224 GLCNKDFFQKMKETA 238
>sp|P45250|YF56_HAEIN Putative 2-hydroxyacid dehydrogenase HI1556
Length = 315
Score = 43.9 bits (102), Expect = 6e-04
Identities = 22/62 (35%), Positives = 35/62 (56%)
Frame = +1
Query: 724 LGMKIMYHQRNKKSNSLFPYYETLNEMLPEVDFLVISCPLTNQTKHFINLDRMKLMKPTA 903
+GMK++Y + +K + Y +E+L + D + + CPLT TK IN + + MK A
Sbjct: 168 VGMKVLYAE-HKDATVCREGYTPFDEVLKQADIVTLHCPLTETTKDLINAETLSKMKKGA 226
Query: 904 FL 909
FL
Sbjct: 227 FL 228
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,951,045
Number of Sequences: 369166
Number of extensions: 1733721
Number of successful extensions: 4016
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3853
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4010
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9222552960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)