Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_D08
(655 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P05165|PCCA_HUMAN Propionyl-CoA carboxylase alpha chain,... 140 2e-33
sp|Q91ZA3|PCCA_MOUSE Propionyl-CoA carboxylase alpha chain,... 130 2e-30
sp|P14882|PCCA_RAT Propionyl-CoA carboxylase alpha chain, m... 127 3e-29
sp|P29337|BCCP_STRMU Biotin carboxyl carrier protein (BCCP) 69 1e-11
sp|Q05920|PYC_MOUSE Pyruvate carboxylase, mitochondrial pre... 68 2e-11
sp|P52873|PYC_RAT Pyruvate carboxylase, mitochondrial precu... 67 5e-11
sp|P11498|PYC_HUMAN Pyruvate carboxylase, mitochondrial pre... 67 5e-11
sp|Q9ZAA7|GCDC_ACIFE Glutaconyl-CoA decarboxylase gamma sub... 64 4e-10
sp|Q99MR8|MCCA_MOUSE Methylcrotonoyl-CoA carboxylase alpha ... 56 7e-08
sp|Q42777|MCCA_SOYBN Methylcrotonoyl-CoA carboxylase alpha ... 56 7e-08
>sp|P05165|PCCA_HUMAN Propionyl-CoA carboxylase alpha chain, mitochondrial precursor
(PCCase alpha subunit) (Propanoyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 703
Score = 140 bits (354), Expect = 2e-33
Identities = 70/158 (44%), Positives = 107/158 (67%)
Frame = +1
Query: 37 VTVSDDRSVFHVAFENGHSFEIKNNFHLADKIIVQNIDNEEIILQLMNRNYSGYVTFQYM 216
V S++ SVF V + G + + ++LA ++ ++D + +Q ++R G ++ Q++
Sbjct: 547 VVASNNGSVFSVEVD-GSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFL 605
Query: 217 GTKYRLKVESILGHSLTKFMLPKKVIDTSSVIIAPMPGLVKSVAVKVGQRVSEGQELCVL 396
GT Y++ + + L L KFML K DTSSV+ +PMPG+V +V+VK G V+EGQE+CV+
Sbjct: 606 GTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVI 665
Query: 397 EAMKMQNSMVAAKTGVIKSIHFQAGQTVSDDDVIIELE 510
EAMKMQNSM A KTG +KS+H QAG TV + D+++ELE
Sbjct: 666 EAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE 703
>sp|Q91ZA3|PCCA_MOUSE Propionyl-CoA carboxylase alpha chain, mitochondrial precursor
(PCCase alpha subunit) (Propanoyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 724
Score = 130 bits (328), Expect = 2e-30
Identities = 63/159 (39%), Positives = 100/159 (62%)
Frame = +1
Query: 34 HVTVSDDRSVFHVAFENGHSFEIKNNFHLADKIIVQNIDNEEIILQLMNRNYSGYVTFQY 213
H V+ + +G + + ++LA ++ N+D + +Q ++R G ++ Q+
Sbjct: 566 HTVVASNNGPAFTVEVDGSKLNVTSTWNLASPLLSVNVDGTQRTVQCLSREAGGNMSIQF 625
Query: 214 MGTKYRLKVESILGHSLTKFMLPKKVIDTSSVIIAPMPGLVKSVAVKVGQRVSEGQELCV 393
+GT Y++ + + L L KFML K DTSS + +PMPG+V +V+VK G V+EGQE+CV
Sbjct: 626 LGTVYKVHILTKLAAELNKFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICV 685
Query: 394 LEAMKMQNSMVAAKTGVIKSIHFQAGQTVSDDDVIIELE 510
+EAMKMQNSM A K G +K +H +AG TV + D+++ELE
Sbjct: 686 IEAMKMQNSMTAGKMGKVKLVHCKAGDTVGEGDLLVELE 724
>sp|P14882|PCCA_RAT Propionyl-CoA carboxylase alpha chain, mitochondrial precursor
(PCCase alpha subunit) (Propanoyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 704
Score = 127 bits (319), Expect = 3e-29
Identities = 64/158 (40%), Positives = 103/158 (65%)
Frame = +1
Query: 37 VTVSDDRSVFHVAFENGHSFEIKNNFHLADKIIVQNIDNEEIILQLMNRNYSGYVTFQYM 216
V S++ F+V + G + + ++LA ++ N+D + +Q ++ + G ++ Q++
Sbjct: 548 VVASNNGPTFNVEVD-GSKLNVTSTWNLASPLLSVNVDGTQRTVQCLSPDAGGNMSIQFL 606
Query: 217 GTKYRLKVESILGHSLTKFMLPKKVIDTSSVIIAPMPGLVKSVAVKVGQRVSEGQELCVL 396
GT Y++ + + L L KFML K DTSSV+ +P PG+V +V+VK G V+EGQE+CV+
Sbjct: 607 GTVYKVHILTKLAAELNKFMLEKVPKDTSSVLRSPKPGVVVAVSVKPGDMVAEGQEICVI 666
Query: 397 EAMKMQNSMVAAKTGVIKSIHFQAGQTVSDDDVIIELE 510
EAMKMQNSM A K G +K +H +AG TV + D+++ELE
Sbjct: 667 EAMKMQNSMTAGKMGKVKLVHCKAGDTVGEGDLLVELE 704
>sp|P29337|BCCP_STRMU Biotin carboxyl carrier protein (BCCP)
Length = 130
Score = 68.6 bits (166), Expect = 1e-11
Identities = 33/76 (43%), Positives = 46/76 (60%)
Frame = +1
Query: 280 PKKVIDTSSVIIAPMPGLVKSVAVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIH 459
P ++ + APMPG + V V VG VSE Q L +LEAMKM+N +VA G + +IH
Sbjct: 54 PAATASAANTMPAPMPGTILKVLVNVGDTVSENQPLMILEAMKMENEIVAGMAGTVSAIH 113
Query: 460 FQAGQTVSDDDVIIEL 507
+GQTV+ D +I +
Sbjct: 114 VSSGQTVNAGDNLITI 129
>sp|Q05920|PYC_MOUSE Pyruvate carboxylase, mitochondrial precursor (Pyruvic carboxylase)
(PCB)
Length = 1178
Score = 67.8 bits (164), Expect = 2e-11
Identities = 32/77 (41%), Positives = 46/77 (59%)
Frame = +1
Query: 280 PKKVIDTSSVIIAPMPGLVKSVAVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIH 459
PK + D I APMPG V + V G +V++GQ LCVL AMKM+ + + G I+ +H
Sbjct: 1102 PKALKDVKGQIGAPMPGKVIDIKVAAGDKVAKGQPLCVLSAMKMETVVTSPMEGTIRKVH 1161
Query: 460 FQAGQTVSDDDVIIELE 510
T+ DD+I+E+E
Sbjct: 1162 VTKDMTLEGDDLILEIE 1178
>sp|P52873|PYC_RAT Pyruvate carboxylase, mitochondrial precursor (Pyruvic carboxylase)
(PCB)
Length = 1178
Score = 66.6 bits (161), Expect = 5e-11
Identities = 33/77 (42%), Positives = 45/77 (58%)
Frame = +1
Query: 280 PKKVIDTSSVIIAPMPGLVKSVAVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIH 459
PK + D I APMPG V V V G +V +GQ LCVL AMKM+ + + G I+ +H
Sbjct: 1102 PKALKDVKGQIGAPMPGKVIDVKVAAGAKVVKGQPLCVLSAMKMETVVTSPMEGTIRKVH 1161
Query: 460 FQAGQTVSDDDVIIELE 510
T+ DD+I+E+E
Sbjct: 1162 VTKDMTLEGDDLILEIE 1178
>sp|P11498|PYC_HUMAN Pyruvate carboxylase, mitochondrial precursor (Pyruvic carboxylase)
(PCB)
Length = 1178
Score = 66.6 bits (161), Expect = 5e-11
Identities = 31/77 (40%), Positives = 46/77 (59%)
Frame = +1
Query: 280 PKKVIDTSSVIIAPMPGLVKSVAVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIH 459
PK + D I APMPG V + V G +V++GQ LCVL AMKM+ + + G ++ +H
Sbjct: 1102 PKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVH 1161
Query: 460 FQAGQTVSDDDVIIELE 510
T+ DD+I+E+E
Sbjct: 1162 VTKDMTLEGDDLILEIE 1178
>sp|Q9ZAA7|GCDC_ACIFE Glutaconyl-CoA decarboxylase gamma subunit (Biotin carrier)
Length = 145
Score = 63.5 bits (153), Expect = 4e-10
Identities = 31/69 (44%), Positives = 43/69 (62%)
Frame = +1
Query: 301 SSVIIAPMPGLVKSVAVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIKSIHFQAGQTV 480
S + APMPG + SV VK G +V G L +LEAMKMQN ++A + G + + AG TV
Sbjct: 77 SVTVSAPMPGKILSVNVKPGDKVEAGDVLLILEAMKMQNEIMAPEDGTVSEVRVNAGDTV 136
Query: 481 SDDDVIIEL 507
+ DV++ L
Sbjct: 137 ATGDVMVIL 145
>sp|Q99MR8|MCCA_MOUSE Methylcrotonoyl-CoA carboxylase alpha chain, mitochondrial
precursor (3-Methylcrotonyl-CoA carboxylase 1) (MCCase
alpha subunit) (3-methylcrotonyl-CoA:carbon dioxide
ligase alpha subunit) (3-Methylcrotonyl-CoA carboxylase
biotin-containing subunit)
Length = 717
Score = 56.2 bits (134), Expect = 7e-08
Identities = 30/87 (34%), Positives = 45/87 (51%)
Frame = +1
Query: 250 LGHSLTKFMLPKKVIDTSSVIIAPMPGLVKSVAVKVGQRVSEGQELCVLEAMKMQNSMVA 429
+G + K++ P IAPM G ++ V VK G RV G L V+ AMKM++++ A
Sbjct: 625 VGIPVPKYLSPVSAEGAQGGTIAPMTGTIEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKA 684
Query: 430 AKTGVIKSIHFQAGQTVSDDDVIIELE 510
K G IK + F G + ++E E
Sbjct: 685 PKDGRIKKVFFSEGAQANRHAPLVEFE 711
>sp|Q42777|MCCA_SOYBN Methylcrotonoyl-CoA carboxylase alpha chain, mitochondrial
precursor (3-Methylcrotonyl-CoA carboxylase 1) (MCCase
alpha subunit) (3-methylcrotonyl-CoA:carbon dioxide
ligase alpha subunit)
Length = 731
Score = 56.2 bits (134), Expect = 7e-08
Identities = 30/80 (37%), Positives = 47/80 (58%), Gaps = 4/80 (5%)
Frame = +1
Query: 283 KKVIDTSS----VIIAPMPGLVKSVAVKVGQRVSEGQELCVLEAMKMQNSMVAAKTGVIK 450
K ++TS+ ++APM GLV V V+ RV EGQ + VLEAMKM++ + A +G +
Sbjct: 650 KPKVETSANPQGTVVAPMAGLVVKVLVENKTRVEEGQPVLVLEAMKMEHVVKAPSSGYVH 709
Query: 451 SIHFQAGQTVSDDDVIIELE 510
+ G+ VSD V+ ++
Sbjct: 710 GLQLMVGEQVSDGSVLFSVK 729
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,671,435
Number of Sequences: 369166
Number of extensions: 1282072
Number of successful extensions: 2942
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2876
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2941
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5413810770
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)