Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_014_C10 (340 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|O77459|KEN_DROME Probable transcription factor Ken (Ken ... 29 3.0 sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinas... 29 3.0 sp|Q08047|GLGB_MAIZE 1,4-alpha-glucan branching enzyme IIB,... 28 8.6
>sp|O77459|KEN_DROME Probable transcription factor Ken (Ken and Barbie protein) Length = 601 Score = 29.3 bits (64), Expect = 3.0 Identities = 17/58 (29%), Positives = 26/58 (44%) Frame = +3 Query: 153 DSFGESSKIAKYNPATDQNDNPQFSAIAGDLNNWKQTASDSDDFLSKNNQAIKSKTKI 326 DS ES A ++ A + NDN + +A D DD K+N++ K +I Sbjct: 164 DSVDESRNAADFSSAFNSNDNCESAAECERSGGHNNKEEDEDDCTHKDNKSDKDTDEI 221
>sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase kinase 4) (MEK kinase 4) (MEKK 4) (MAP three kinase 1) Length = 1607 Score = 29.3 bits (64), Expect = 3.0 Identities = 16/46 (34%), Positives = 21/46 (45%) Frame = -1 Query: 274 ESDAVCFQLFKSPAIAEN*GLSF*SVAGLYLAIFEDSPKESALPLC 137 E D + Q + I G V GLYLAI +SP+ +P C Sbjct: 1112 EDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPMKVPRC 1157
>sp|Q08047|GLGB_MAIZE 1,4-alpha-glucan branching enzyme IIB, chloroplast precursor (Starch branching enzyme IIB) (Q-enzyme) Length = 799 Score = 27.7 bits (60), Expect = 8.6 Identities = 17/60 (28%), Positives = 29/60 (48%), Gaps = 6/60 (10%) Frame = +3 Query: 135 EHSGSADSFGESSKIAKYNPATDQNDNPQFS------AIAGDLNNWKQTASDSDDFLSKN 296 EH G ++F S + +N + + +++ A+ GD+NNW A D +SKN Sbjct: 151 EHEGGLEAFSRSYEKFGFNASAEGITYREWAPGAFSAALVGDVNNWDPNA----DRMSKN 206
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 33,906,974 Number of Sequences: 369166 Number of extensions: 525751 Number of successful extensions: 1297 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 1282 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1296 length of database: 68,354,980 effective HSP length: 80 effective length of database: 53,576,180 effective search space used: 1714437760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)