Planarian EST Database


Dr_sW_014_C04

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_014_C04
         (484 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9NQC7|CYLD_HUMAN  Probable ubiquitin carboxyl-terminal h...    69   4e-12
sp|Q80TQ2|CYLD_MOUSE  Probable ubiquitin carboxyl-terminal h...    67   2e-11
sp|P55241|GLGL1_MAIZE  Glucose-1-phosphate adenylyltransfera...    32   0.77 
sp|Q58554|YB54_METJA  Hypothetical protein MJ1154                  31   1.3  
sp|Q9V8W3|RABEP_DROME  Rab proteins geranylgeranyltransferas...    30   2.2  
sp|P57504|MUTS_BUCAI  DNA mismatch repair protein mutS             29   5.0  
sp|P30284|CG21_EMENI  G2/mitotic-specific cyclin-B                 29   6.5  
sp|P32767|PDR6_YEAST  Pleiotropic drug resistance regulatory...    28   8.5  
sp|P31231|CASQ1_RANES  Calsequestrin-1 precursor (Calsequest...    28   8.5  
sp|Q9Y227|ENP4_HUMAN  Ectonucleoside triphosphate diphosphoh...    28   8.5  
>sp|Q9NQC7|CYLD_HUMAN Probable ubiquitin carboxyl-terminal hydrolase CYLD (Ubiquitin
           thiolesterase CYLD) (Ubiquitin specific-processing
           protease CYLD) (Deubiquitinating enzyme CYLD)
          Length = 956

 Score = 69.3 bits (168), Expect = 4e-12
 Identities = 41/116 (35%), Positives = 61/116 (52%), Gaps = 2/116 (1%)
 Frame = +2

Query: 44  EIVNILRSKIIFPLRKYEYVSFKSVSEFRQILGTLYPDKKFLHSEKDPEELLETLFHSIT 223
           E   +LR++I+ PLR Y YV    + + R+IL  +     F   EKDPEE L  LFH I 
Sbjct: 635 ETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHHIL 694

Query: 224 PNDPFLRTYDIKSA--NFQNCFMTQLFYTNKFNSSVPTIKEMLNRTFLEGGILIDE 385
             +P L+   I+SA    Q+C+  Q+F        VPTI+++L  +F+   +   E
Sbjct: 695 RVEPLLK---IRSAGQKVQDCYFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAE 747
>sp|Q80TQ2|CYLD_MOUSE Probable ubiquitin carboxyl-terminal hydrolase CYLD (Ubiquitin
           thiolesterase CYLD) (Ubiquitin specific-processing
           protease CYLD) (Deubiquitinating enzyme CYLD)
          Length = 952

 Score = 67.4 bits (163), Expect = 2e-11
 Identities = 41/116 (35%), Positives = 60/116 (51%), Gaps = 2/116 (1%)
 Frame = +2

Query: 44  EIVNILRSKIIFPLRKYEYVSFKSVSEFRQILGTLYPDKKFLHSEKDPEELLETLFHSIT 223
           E   +LR++I+ PLR Y YV    + + R+IL  +     F   EKDPEE L  LFH I 
Sbjct: 631 ETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHDIL 690

Query: 224 PNDPFLRTYDIKSA--NFQNCFMTQLFYTNKFNSSVPTIKEMLNRTFLEGGILIDE 385
             +P L+   I+SA    Q+C   Q+F        VPTI+++L  +F+   +   E
Sbjct: 691 RVEPLLK---IRSAGQKVQDCNFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAE 743
>sp|P55241|GLGL1_MAIZE Glucose-1-phosphate adenylyltransferase large subunit 1,
           chloroplast precursor (ADP-glucose synthase)
           (ADP-glucose pyrophosphorylase) (AGPASE S)
           (Alpha-D-glucose-1-phosphate adenyl transferase)
           (Shrunken-2)
          Length = 516

 Score = 32.0 bits (71), Expect = 0.77
 Identities = 16/44 (36%), Positives = 28/44 (63%)
 Frame = +2

Query: 242 RTYDIKSANFQNCFMTQLFYTNKFNSSVPTIKEMLNRTFLEGGI 373
           R  DI  +N  N  + ++F  ++FNS+  ++   ++RT+LEGGI
Sbjct: 116 RLIDIPMSNCFNSGINKIFVMSQFNST--SLNRHIHRTYLEGGI 157
>sp|Q58554|YB54_METJA Hypothetical protein MJ1154
          Length = 451

 Score = 31.2 bits (69), Expect = 1.3
 Identities = 27/93 (29%), Positives = 41/93 (44%), Gaps = 2/93 (2%)
 Frame = +2

Query: 119 SEFRQILGTLYPDKKFLHSEKDPEEL--LETLFHSITPNDPFLRTYDIKSANFQNCFMTQ 292
           + F   LGT++   K         EL  +  L H I  + PF  T +I   + +     +
Sbjct: 49  TRFEHSLGTMFIASKIAEKINADVELTRVSALLHDIG-HPPFSHTLEICGYSHEVFGRKK 107

Query: 293 LFYTNKFNSSVPTIKEMLNRTFLEGGILIDEVD 391
           + + N  N S   I + LNR  LEG I+  +VD
Sbjct: 108 IKHMNLDNFSKSEIIKTLNRKNLEGKIISGDVD 140
>sp|Q9V8W3|RABEP_DROME Rab proteins geranylgeranyltransferase component A (Rab escort
           protein homolog) (REP)
          Length = 511

 Score = 30.4 bits (67), Expect = 2.2
 Identities = 18/64 (28%), Positives = 29/64 (45%), Gaps = 6/64 (9%)
 Frame = +2

Query: 92  YEYVSFKSVSEFRQILGTLYPDKKFLHSEKDPEELL------ETLFHSITPNDPFLRTYD 253
           YE     +++  R I G ++PD  FL    DPEE++        L     P D   + +D
Sbjct: 440 YELDYDAAIANARDIFGKMFPDADFLPRAPDPEEIVVDGEDPSALNEHTLPEDLRAQLHD 499

Query: 254 IKSA 265
           ++ A
Sbjct: 500 MQQA 503
>sp|P57504|MUTS_BUCAI DNA mismatch repair protein mutS
          Length = 802

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 26/110 (23%), Positives = 53/110 (48%), Gaps = 3/110 (2%)
 Frame = +2

Query: 86  RKYEYVSFKSVSEFRQILGT-LYPDKKFLHSEKDPEELLETLFHSITPNDPFLRTYDIKS 262
           +K    SFK   +F +I+G  +   K+  H       +++TL ++   + P L+ Y+ K 
Sbjct: 459 KKLMIESFKI--KFNKIIGYYIQISKRHTHLIPKYYVIIQTLKNTERYSVPLLKEYEEKV 516

Query: 263 AN--FQNCFMTQLFYTNKFNSSVPTIKEMLNRTFLEGGILIDEVDLLVNM 406
            N   ++  + +  Y   FN   P ++++ N       + + E+D+LVN+
Sbjct: 517 LNSEMRSLLLEKKLYAEIFNIIEPFLEKLQN-----SALALSELDVLVNL 561
>sp|P30284|CG21_EMENI G2/mitotic-specific cyclin-B
          Length = 478

 Score = 28.9 bits (63), Expect = 6.5
 Identities = 34/156 (21%), Positives = 65/156 (41%), Gaps = 28/156 (17%)
 Frame = +2

Query: 35  INQEIVNILRSKII-----FPLRKYEYVSFKSVSEFRQILGTLYPDKKFLHSEKDPEELL 199
           ++ E+V + R +++     F   KYE V    V+ F  +    + DK+ L +E+    +L
Sbjct: 273 LSAEVVALDRLQLVGVAAMFIASKYEEVLSPHVANFSHVADETFSDKEILDAER---HIL 329

Query: 200 ETLFHSITPNDP--FLR------TYDIKSANFQNCFMTQLFYTNKF----NSSVPTIKEM 343
            TL ++++  +P  FLR       YDI++       M      ++F     S +      
Sbjct: 330 ATLEYNMSYPNPMNFLRRISKADNYDIQTRTLGKYLMEISLLDHRFLGYPQSQIGAAAMY 389

Query: 344 LNRTFLE-----------GGILIDEVDLLVNMLIHY 418
           L R  L+            G   +E+D +  +++ Y
Sbjct: 390 LARLILDRGPWDATLAHYAGYTEEEIDEVFRLMVDY 425
>sp|P32767|PDR6_YEAST Pleiotropic drug resistance regulatory protein 6
          Length = 1081

 Score = 28.5 bits (62), Expect = 8.5
 Identities = 12/45 (26%), Positives = 27/45 (60%)
 Frame = +2

Query: 125 FRQILGTLYPDKKFLHSEKDPEELLETLFHSITPNDPFLRTYDIK 259
           F+ ++  +Y   +     ++ E++L T+F ++  NDP L +YD++
Sbjct: 276 FQNLIDLMYQSTEGSDGYENAEKIL-TIFGNVFANDPLLMSYDLR 319
>sp|P31231|CASQ1_RANES Calsequestrin-1 precursor (Calsequestrin, skeletal muscle isoform)
          Length = 420

 Score = 28.5 bits (62), Expect = 8.5
 Identities = 20/109 (18%), Positives = 45/109 (41%)
 Frame = +2

Query: 23  STQNINQEIVNILRSKIIFPLRKYEYVSFKSVSEFRQILGTLYPDKKFLHSEKDPEELLE 202
           S   + + ++++L   + F    +E  +F+++ +  +++G       F + + +  +  E
Sbjct: 133 SADTLVEFLLDVLEDPVEFIDGSHELAAFENLDDEPKLIGY------FKNEDSEHYKAYE 186

Query: 203 TLFHSITPNDPFLRTYDIKSANFQNCFMTQLFYTNKFNSSVPTIKEMLN 349
                  P  PF  T+D K A      + ++ Y   F+    TI    N
Sbjct: 187 DAAEEFHPYIPFFATFDAKVAKTLTLKLNEIDYYEPFHDEPITIPSKPN 235
>sp|Q9Y227|ENP4_HUMAN Ectonucleoside triphosphate diphosphohydrolase 4 (NTPDase4)
           (Uridine-diphosphatase) (UDPase) (Lysosomal apyrase-like
           protein of 70 kDa)
          Length = 616

 Score = 28.5 bits (62), Expect = 8.5
 Identities = 25/96 (26%), Positives = 41/96 (42%), Gaps = 7/96 (7%)
 Frame = +2

Query: 218 ITPNDPFLRT---YDIKSANFQNCFMTQLFYTNKFNSSVPTIKEMLNRTFLEGGILIDEV 388
           +TP+ P+L      DIK    QN     L  T  F+    TI+  +N+T        +E 
Sbjct: 358 LTPDMPYLDPCLPLDIKDEIQQNGQTIYLRGTGDFDLCRETIQPFMNKT--------NET 409

Query: 389 DLLVNML----IHYQSKMISNLVSLFVCKIEILRLG 484
              +N +    IH+Q+         + C  ++LR+G
Sbjct: 410 QTSLNGVYQPPIHFQNSEFYGFSEFYYCTEDVLRMG 445
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,739,959
Number of Sequences: 369166
Number of extensions: 934497
Number of successful extensions: 2349
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2304
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2347
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2871696580
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)